This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 106.50 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 385,025 (12.1%) Reads that were too short: 21,275 (0.7%) Reads written (passing filters): 363,750 (11.4%) Total basepairs processed: 961,105,341 bp Total written (filtered): 54,924,000 bp (5.7%) === Adapter 1 === Sequence: CACTCT; Type: regular 5'; Length: 6; Trimmed: 385025 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 327 779.6 0 327 7 3869 779.6 0 3869 8 208 779.6 0 208 9 13363 779.6 0 13363 10 35 779.6 0 35 11 14 779.6 0 14 12 17 779.6 0 17 13 12 779.6 0 12 14 10 779.6 0 10 15 14 779.6 0 14 16 10 779.6 0 10 17 21 779.6 0 21 18 32 779.6 0 32 19 11 779.6 0 11 20 6 779.6 0 6 21 7 779.6 0 7 22 9 779.6 0 9 23 18 779.6 0 18 24 6 779.6 0 6 25 7 779.6 0 7 26 11 779.6 0 11 27 6 779.6 0 6 28 15 779.6 0 15 29 6 779.6 0 6 30 10 779.6 0 10 31 26 779.6 0 26 32 38 779.6 0 38 33 20 779.6 0 20 34 34 779.6 0 34 35 330 779.6 0 330 36 12 779.6 0 12 37 27 779.6 0 27 38 9 779.6 0 9 39 13 779.6 0 13 40 13 779.6 0 13 41 10 779.6 0 10 42 116 779.6 0 116 43 13 779.6 0 13 44 17 779.6 0 17 45 29 779.6 0 29 46 136 779.6 0 136 47 247 779.6 0 247 48 165 779.6 0 165 49 42 779.6 0 42 50 135 779.6 0 135 51 17 779.6 0 17 52 17 779.6 0 17 53 16 779.6 0 16 54 114 779.6 0 114 55 36 779.6 0 36 56 44 779.6 0 44 57 14 779.6 0 14 58 15 779.6 0 15 59 23 779.6 0 23 60 12 779.6 0 12 61 15 779.6 0 15 62 33 779.6 0 33 63 15 779.6 0 15 64 9 779.6 0 9 65 11 779.6 0 11 66 12 779.6 0 12 67 25 779.6 0 25 68 7 779.6 0 7 69 14 779.6 0 14 70 27 779.6 0 27 71 19 779.6 0 19 72 3 779.6 0 3 73 8 779.6 0 8 74 12 779.6 0 12 75 3 779.6 0 3 76 7 779.6 0 7 77 13 779.6 0 13 78 14 779.6 0 14 79 22 779.6 0 22 80 22 779.6 0 22 81 16 779.6 0 16 82 38 779.6 0 38 83 39 779.6 0 39 84 31 779.6 0 31 85 11 779.6 0 11 86 10 779.6 0 10 87 18 779.6 0 18 88 18 779.6 0 18 89 33 779.6 0 33 90 62 779.6 0 62 91 9 779.6 0 9 92 143 779.6 0 143 93 59 779.6 0 59 94 165 779.6 0 165 95 58 779.6 0 58 96 45 779.6 0 45 97 43 779.6 0 43 98 41 779.6 0 41 99 34 779.6 0 34 100 48 779.6 0 48 101 21 779.6 0 21 102 67 779.6 0 67 103 52 779.6 0 52 104 176 779.6 0 176 105 528 779.6 0 528 106 655 779.6 0 655 107 42 779.6 0 42 108 99 779.6 0 99 109 93 779.6 0 93 110 139 779.6 0 139 111 55 779.6 0 55 112 204 779.6 0 204 113 99 779.6 0 99 114 114 779.6 0 114 115 32 779.6 0 32 116 37 779.6 0 37 117 40 779.6 0 40 118 56 779.6 0 56 119 138 779.6 0 138 120 41 779.6 0 41 121 46 779.6 0 46 122 59 779.6 0 59 123 31 779.6 0 31 124 122 779.6 0 122 125 127 779.6 0 127 126 89 779.6 0 89 127 235 779.6 0 235 128 258 779.6 0 258 129 419 779.6 0 419 130 136 779.6 0 136 131 247 779.6 0 247 132 78 779.6 0 78 133 157 779.6 0 157 134 878 779.6 0 878 135 219 779.6 0 219 136 217 779.6 0 217 137 92 779.6 0 92 138 104 779.6 0 104 139 184 779.6 0 184 140 247 779.6 0 247 141 296 779.6 0 296 142 354 779.6 0 354 143 643 779.6 0 643 144 1721 779.6 0 1721 145 1623 779.6 0 1623 146 22266 779.6 0 22266 147 10971 779.6 0 10971 148 26982 779.6 0 26982 149 514 779.6 0 514 150 291 779.6 0 291 151 912 779.6 0 912 152 1052 779.6 0 1052 153 2235 779.6 0 2235 154 430 779.6 0 430 155 499 779.6 0 499 156 826 779.6 0 826 157 1220 779.6 0 1220 158 1546 779.6 0 1546 159 3914 779.6 0 3914 160 6416 779.6 0 6416 161 104459 779.6 0 104459 162 62973 779.6 0 62973 163 79950 779.6 0 79950 164 552 779.6 0 552 165 230 779.6 0 230 166 156 779.6 0 156 167 145 779.6 0 145 168 57 779.6 0 57 169 40 779.6 0 40 170 33 779.6 0 33 171 46 779.6 0 46 172 68 779.6 0 68 173 107 779.6 0 107 174 79 779.6 0 79 175 46 779.6 0 46 176 54 779.6 0 54 177 45 779.6 0 45 178 38 779.6 0 38 179 92 779.6 0 92 180 82 779.6 0 82 181 145 779.6 0 145 182 299 779.6 0 299 183 369 779.6 0 369 184 169 779.6 0 169 185 72 779.6 0 72 186 50 779.6 0 50 187 170 779.6 0 170 188 40 779.6 0 40 189 78 779.6 0 78 190 85 779.6 0 85 191 85 779.6 0 85 192 127 779.6 0 127 193 62 779.6 0 62 194 55 779.6 0 55 195 31 779.6 0 31 196 62 779.6 0 62 197 58 779.6 0 58 198 67 779.6 0 67 199 50 779.6 0 50 200 28 779.6 0 28 201 56 779.6 0 56 202 132 779.6 0 132 203 107 779.6 0 107 204 111 779.6 0 111 205 58 779.6 0 58 206 79 779.6 0 79 207 49 779.6 0 49 208 50 779.6 0 50 209 82 779.6 0 82 210 52 779.6 0 52 211 62 779.6 0 62 212 58 779.6 0 58 213 100 779.6 0 100 214 136 779.6 0 136 215 173 779.6 0 173 216 167 779.6 0 167 217 160 779.6 0 160 218 244 779.6 0 244 219 163 779.6 0 163 220 90 779.6 0 90 221 70 779.6 0 70 222 120 779.6 0 120 223 182 779.6 0 182 224 69 779.6 0 69 225 114 779.6 0 114 226 170 779.6 0 170 227 106 779.6 0 106 228 153 779.6 0 153 229 90 779.6 0 90 230 68 779.6 0 68 231 56 779.6 0 56 232 63 779.6 0 63 233 91 779.6 0 91 234 106 779.6 0 106 235 75 779.6 0 75 236 76 779.6 0 76 237 94 779.6 0 94 238 293 779.6 0 293 239 262 779.6 0 262 240 1582 779.6 0 1582 241 1769 779.6 0 1769 242 3852 779.6 0 3852 243 346 779.6 0 346 244 278 779.6 0 278 245 149 779.6 0 149 246 100 779.6 0 100 247 35 779.6 0 35 248 35 779.6 0 35 249 55 779.6 0 55 250 120 779.6 0 120 251 164 779.6 0 164 252 212 779.6 0 212 253 153 779.6 0 153 254 255 779.6 0 255 255 169 779.6 0 169 256 152 779.6 0 152 257 91 779.6 0 91 258 53 779.6 0 53 259 37 779.6 0 37 260 51 779.6 0 51 261 103 779.6 0 103 262 148 779.6 0 148 263 133 779.6 0 133 264 122 779.6 0 122 265 160 779.6 0 160 266 202 779.6 0 202 267 415 779.6 0 415 268 315 779.6 0 315 269 336 779.6 0 336 270 338 779.6 0 338 271 241 779.6 0 241 272 220 779.6 0 220 273 119 779.6 0 119 274 99 779.6 0 99 275 184 779.6 0 184 276 191 779.6 0 191 277 745 779.6 0 745 278 905 779.6 0 905 279 477 779.6 0 477 280 231 779.6 0 231 281 133 779.6 0 133 282 123 779.6 0 123 283 243 779.6 0 243 284 90 779.6 0 90 285 116 779.6 0 116 286 80 779.6 0 80 287 61 779.6 0 61 288 76 779.6 0 76 289 44 779.6 0 44 290 97 779.6 0 97 291 103 779.6 0 103 292 78 779.6 0 78 293 46 779.6 0 46 294 33 779.6 0 33 295 14 779.6 0 14 296 19 779.6 0 19 297 32 779.6 0 32 298 29 779.6 0 29 299 63 779.6 0 63 300 67 779.6 0 67 301 55 779.6 0 55 Finished in 13.64 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 363,750 Reads with adapters: 13,745 (3.8%) Reads that were too short: 33 (0.0%) Reads written (passing filters): 13,712 (3.8%) Total basepairs processed: 54,924,000 bp Total written (filtered): 3,765,596 bp (6.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13745 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 10 0.0 3 0 0 9 1 16 142 0.0 3 0 139 3 17 13463 0.0 3 13162 296 4 1 18 35 0.0 3 2 30 3 19 15 0.0 3 1 0 11 3 20 11 0.0 3 0 0 0 11 29 1 0.0 3 0 0 0 1 30 1 0.0 3 1 45 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 4 0.0 3 0 0 0 4 53 4 0.0 3 0 0 0 4 55 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 73 3 0.0 3 0 0 0 3 87 4 0.0 3 0 0 0 4 90 4 0.0 3 0 0 0 4 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 4 0.0 3 0 0 0 4 128 9 0.0 3 0 0 0 9 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 12 0.0 3 0 0 0 12 138 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.62 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 13,712 Reads with adapters: 666 (4.9%) Reads written (passing filters): 13,712 (100.0%) Total basepairs processed: 3,765,596 bp Total written (filtered): 3,763,595 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 666 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.2% C: 3.6% G: 24.9% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 663 214.2 0 663 4 3 53.6 0 3