This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCTAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 103.54 s (33 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 260,521 (8.2%) Reads that were too short: 91,660 (2.9%) Reads written (passing filters): 168,861 (5.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 29,672,294 bp (3.1%) === Adapter 1 === Sequence: GGCTAC; Type: regular 5'; Length: 6; Trimmed: 260521 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 16 777.6 0 16 7 27 777.6 0 27 8 22 777.6 0 22 9 30 777.6 0 30 10 18 777.6 0 18 11 26 777.6 0 26 12 26 777.6 0 26 13 140 777.6 0 140 14 329 777.6 0 329 15 228 777.6 0 228 16 103 777.6 0 103 17 25 777.6 0 25 18 21 777.6 0 21 19 22 777.6 0 22 20 13 777.6 0 13 21 11 777.6 0 11 22 25 777.6 0 25 23 30 777.6 0 30 24 44 777.6 0 44 25 11 777.6 0 11 26 14 777.6 0 14 27 14 777.6 0 14 28 19 777.6 0 19 29 158 777.6 0 158 30 51 777.6 0 51 31 911 777.6 0 911 32 7 777.6 0 7 33 10 777.6 0 10 34 5 777.6 0 5 35 16 777.6 0 16 36 11 777.6 0 11 37 11 777.6 0 11 38 15 777.6 0 15 39 32 777.6 0 32 40 30 777.6 0 30 41 26 777.6 0 26 42 15 777.6 0 15 43 9 777.6 0 9 44 16 777.6 0 16 45 7 777.6 0 7 46 15 777.6 0 15 47 10 777.6 0 10 48 9 777.6 0 9 49 14 777.6 0 14 50 59 777.6 0 59 51 22 777.6 0 22 52 36 777.6 0 36 53 11 777.6 0 11 54 10 777.6 0 10 55 11 777.6 0 11 56 5 777.6 0 5 57 12 777.6 0 12 58 10 777.6 0 10 59 7 777.6 0 7 60 8 777.6 0 8 61 4 777.6 0 4 62 10 777.6 0 10 63 5 777.6 0 5 64 6 777.6 0 6 65 6 777.6 0 6 66 6 777.6 0 6 67 9 777.6 0 9 68 15 777.6 0 15 69 8 777.6 0 8 70 4 777.6 0 4 71 7 777.6 0 7 72 11 777.6 0 11 73 6 777.6 0 6 74 17 777.6 0 17 75 7 777.6 0 7 76 9 777.6 0 9 77 8 777.6 0 8 78 10 777.6 0 10 79 9 777.6 0 9 80 12 777.6 0 12 81 21 777.6 0 21 82 44 777.6 0 44 83 76 777.6 0 76 84 132 777.6 0 132 85 43 777.6 0 43 86 36 777.6 0 36 87 59 777.6 0 59 88 14 777.6 0 14 89 62 777.6 0 62 90 24 777.6 0 24 91 24 777.6 0 24 92 25 777.6 0 25 93 11 777.6 0 11 94 10 777.6 0 10 95 18 777.6 0 18 96 23 777.6 0 23 97 46 777.6 0 46 98 63 777.6 0 63 99 30 777.6 0 30 100 138 777.6 0 138 101 41 777.6 0 41 102 50 777.6 0 50 103 25 777.6 0 25 104 131 777.6 0 131 105 56 777.6 0 56 106 139 777.6 0 139 107 190 777.6 0 190 108 308 777.6 0 308 109 454 777.6 0 454 110 602 777.6 0 602 111 1521 777.6 0 1521 112 212 777.6 0 212 113 281 777.6 0 281 114 532 777.6 0 532 115 288 777.6 0 288 116 292 777.6 0 292 117 512 777.6 0 512 118 386 777.6 0 386 119 630 777.6 0 630 120 816 777.6 0 816 121 1114 777.6 0 1114 122 998 777.6 0 998 123 2718 777.6 0 2718 124 16972 777.6 0 16972 125 115402 777.6 0 115402 126 8660 777.6 0 8660 127 100 777.6 0 100 128 371 777.6 0 371 129 44 777.6 0 44 130 52 777.6 0 52 131 28 777.6 0 28 132 8 777.6 0 8 133 40 777.6 0 40 134 40 777.6 0 40 135 78 777.6 0 78 136 14 777.6 0 14 137 14 777.6 0 14 138 18 777.6 0 18 139 25 777.6 0 25 140 17 777.6 0 17 141 37 777.6 0 37 142 38 777.6 0 38 143 12 777.6 0 12 144 41 777.6 0 41 145 20 777.6 0 20 146 38 777.6 0 38 147 209 777.6 0 209 148 100 777.6 0 100 149 77 777.6 0 77 150 16 777.6 0 16 151 106 777.6 0 106 152 336 777.6 0 336 153 279 777.6 0 279 154 425 777.6 0 425 155 116 777.6 0 116 156 163 777.6 0 163 157 417 777.6 0 417 158 523 777.6 0 523 159 880 777.6 0 880 160 196 777.6 0 196 161 227 777.6 0 227 162 252 777.6 0 252 163 165 777.6 0 165 164 113 777.6 0 113 165 46 777.6 0 46 166 44 777.6 0 44 167 69 777.6 0 69 168 46 777.6 0 46 169 40 777.6 0 40 170 131 777.6 0 131 171 104 777.6 0 104 172 187 777.6 0 187 173 32 777.6 0 32 174 66 777.6 0 66 175 21 777.6 0 21 176 106 777.6 0 106 177 2226 777.6 0 2226 178 1107 777.6 0 1107 179 1363 777.6 0 1363 180 141 777.6 0 141 181 14 777.6 0 14 182 24 777.6 0 24 183 21 777.6 0 21 184 25 777.6 0 25 185 9 777.6 0 9 186 12 777.6 0 12 187 17 777.6 0 17 188 9 777.6 0 9 189 7 777.6 0 7 190 11 777.6 0 11 191 15 777.6 0 15 192 28 777.6 0 28 193 7 777.6 0 7 194 23 777.6 0 23 195 26 777.6 0 26 196 32 777.6 0 32 197 8 777.6 0 8 198 10 777.6 0 10 199 3 777.6 0 3 200 10 777.6 0 10 201 5 777.6 0 5 202 9 777.6 0 9 203 15 777.6 0 15 204 12 777.6 0 12 205 13 777.6 0 13 206 17 777.6 0 17 207 99 777.6 0 99 208 21 777.6 0 21 209 19 777.6 0 19 210 21 777.6 0 21 211 10 777.6 0 10 212 10 777.6 0 10 213 21 777.6 0 21 214 33 777.6 0 33 215 20 777.6 0 20 216 19 777.6 0 19 217 17 777.6 0 17 218 10 777.6 0 10 219 20 777.6 0 20 220 29 777.6 0 29 221 27 777.6 0 27 222 12 777.6 0 12 223 13 777.6 0 13 224 32 777.6 0 32 225 15 777.6 0 15 226 16 777.6 0 16 227 10 777.6 0 10 228 20 777.6 0 20 229 38 777.6 0 38 230 59 777.6 0 59 231 29 777.6 0 29 232 29 777.6 0 29 233 310 777.6 0 310 234 81 777.6 0 81 235 84 777.6 0 84 236 163 777.6 0 163 237 14 777.6 0 14 238 32 777.6 0 32 239 14 777.6 0 14 240 22 777.6 0 22 241 36 777.6 0 36 242 24 777.6 0 24 243 15 777.6 0 15 244 53 777.6 0 53 245 33 777.6 0 33 246 21 777.6 0 21 247 46 777.6 0 46 248 35 777.6 0 35 249 28 777.6 0 28 250 34 777.6 0 34 251 17 777.6 0 17 252 54 777.6 0 54 253 30 777.6 0 30 254 67 777.6 0 67 255 65 777.6 0 65 256 106 777.6 0 106 257 1291 777.6 0 1291 258 360 777.6 0 360 259 1105 777.6 0 1105 260 107 777.6 0 107 261 221 777.6 0 221 262 157 777.6 0 157 263 163 777.6 0 163 264 146 777.6 0 146 265 274 777.6 0 274 266 33 777.6 0 33 267 83 777.6 0 83 268 196 777.6 0 196 269 373 777.6 0 373 270 270 777.6 0 270 271 37 777.6 0 37 272 15 777.6 0 15 273 26 777.6 0 26 274 42 777.6 0 42 275 95 777.6 0 95 276 70 777.6 0 70 277 25 777.6 0 25 278 31 777.6 0 31 279 39 777.6 0 39 280 159 777.6 0 159 281 165 777.6 0 165 282 91 777.6 0 91 283 85 777.6 0 85 284 27 777.6 0 27 285 40 777.6 0 40 286 77 777.6 0 77 287 142 777.6 0 142 288 968 777.6 0 968 289 835 777.6 0 835 290 1069 777.6 0 1069 291 319 777.6 0 319 292 962 777.6 0 962 293 18660 777.6 0 18660 294 35671 777.6 0 35671 295 22048 777.6 0 22048 296 2728 777.6 0 2728 297 65 777.6 0 65 298 24 777.6 0 24 299 138 777.6 0 138 300 90 777.6 0 90 301 39 777.6 0 39 Finished in 7.94 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 168,861 Reads with adapters: 40 (0.0%) Reads that were too short: 37 (0.0%) Reads written (passing filters): 3 (0.0%) Total basepairs processed: 29,672,294 bp Total written (filtered): 806 bp (0.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 40 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 2 0.0 4 2 29 1 0.0 4 1 36 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 63 2 0.0 4 0 0 0 0 2 64 2 0.0 4 0 0 0 0 2 74 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 112 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 117 4 0.0 4 0 0 0 0 4 118 2 0.0 4 0 0 0 0 2 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 166 3 0.0 4 0 0 0 0 3 167 2 0.0 4 0 0 0 0 2 168 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 180 2 0.0 4 0 0 0 0 2 239 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (3333 us/read; 0.02 M reads/minute). === Summary === Total reads processed: 3 Reads with adapters: 0 (0.0%) Reads written (passing filters): 3 (100.0%) Total basepairs processed: 806 bp Total written (filtered): 806 bp (100.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 0 times.