This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 99.72 s (31 us/read; 1.92 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 5,720 (0.2%) Reads that were too short: 2,022 (0.1%) Reads written (passing filters): 3,698 (0.1%) Total basepairs processed: 958,672,960 bp Total written (filtered): 764,818 bp (0.1%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 5720 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 93 777.6 0 93 7 239 777.6 0 239 8 39 777.6 0 39 9 71 777.6 0 71 10 6 777.6 0 6 11 7 777.6 0 7 12 8 777.6 0 8 13 7 777.6 0 7 14 4 777.6 0 4 15 7 777.6 0 7 16 9 777.6 0 9 17 7 777.6 0 7 18 4 777.6 0 4 19 8 777.6 0 8 20 7 777.6 0 7 21 3 777.6 0 3 22 6 777.6 0 6 23 6 777.6 0 6 24 6 777.6 0 6 25 2 777.6 0 2 26 5 777.6 0 5 27 8 777.6 0 8 28 15 777.6 0 15 29 7 777.6 0 7 30 12 777.6 0 12 31 11 777.6 0 11 32 11 777.6 0 11 33 9 777.6 0 9 34 8 777.6 0 8 35 14 777.6 0 14 36 9 777.6 0 9 37 6 777.6 0 6 38 12 777.6 0 12 39 7 777.6 0 7 40 15 777.6 0 15 41 10 777.6 0 10 42 12 777.6 0 12 43 69 777.6 0 69 44 8 777.6 0 8 45 12 777.6 0 12 46 18 777.6 0 18 47 22 777.6 0 22 48 352 777.6 0 352 49 81 777.6 0 81 50 271 777.6 0 271 51 6 777.6 0 6 52 3 777.6 0 3 53 4 777.6 0 4 54 4 777.6 0 4 55 14 777.6 0 14 56 3 777.6 0 3 57 6 777.6 0 6 58 2 777.6 0 2 59 8 777.6 0 8 60 3 777.6 0 3 61 6 777.6 0 6 62 5 777.6 0 5 63 7 777.6 0 7 64 8 777.6 0 8 65 39 777.6 0 39 66 12 777.6 0 12 67 4 777.6 0 4 68 10 777.6 0 10 69 5 777.6 0 5 70 5 777.6 0 5 71 8 777.6 0 8 72 4 777.6 0 4 73 4 777.6 0 4 74 5 777.6 0 5 75 1 777.6 0 1 76 3 777.6 0 3 77 9 777.6 0 9 78 5 777.6 0 5 79 1 777.6 0 1 80 7 777.6 0 7 81 7 777.6 0 7 82 3 777.6 0 3 83 22 777.6 0 22 84 62 777.6 0 62 85 68 777.6 0 68 86 9 777.6 0 9 87 2 777.6 0 2 88 4 777.6 0 4 89 11 777.6 0 11 90 6 777.6 0 6 91 2 777.6 0 2 92 3 777.6 0 3 93 4 777.6 0 4 94 1 777.6 0 1 95 25 777.6 0 25 96 356 777.6 0 356 97 6 777.6 0 6 98 9 777.6 0 9 99 2 777.6 0 2 100 3 777.6 0 3 101 2 777.6 0 2 102 5 777.6 0 5 103 4 777.6 0 4 104 6 777.6 0 6 105 3 777.6 0 3 106 4 777.6 0 4 107 3 777.6 0 3 108 2 777.6 0 2 109 2 777.6 0 2 110 1 777.6 0 1 111 4 777.6 0 4 112 2 777.6 0 2 113 3 777.6 0 3 114 4 777.6 0 4 115 5 777.6 0 5 116 2 777.6 0 2 117 5 777.6 0 5 118 18 777.6 0 18 119 14 777.6 0 14 120 31 777.6 0 31 121 6 777.6 0 6 122 2 777.6 0 2 123 1 777.6 0 1 124 1 777.6 0 1 125 3 777.6 0 3 126 1 777.6 0 1 127 3 777.6 0 3 128 5 777.6 0 5 129 4 777.6 0 4 130 4 777.6 0 4 131 4 777.6 0 4 132 19 777.6 0 19 133 5 777.6 0 5 134 4 777.6 0 4 135 6 777.6 0 6 136 9 777.6 0 9 137 8 777.6 0 8 138 8 777.6 0 8 139 7 777.6 0 7 140 7 777.6 0 7 141 9 777.6 0 9 142 9 777.6 0 9 143 5 777.6 0 5 144 8 777.6 0 8 145 14 777.6 0 14 146 5 777.6 0 5 147 23 777.6 0 23 148 74 777.6 0 74 149 35 777.6 0 35 150 6 777.6 0 6 151 3 777.6 0 3 152 4 777.6 0 4 153 7 777.6 0 7 154 119 777.6 0 119 155 5 777.6 0 5 156 3 777.6 0 3 157 2 777.6 0 2 158 3 777.6 0 3 159 3 777.6 0 3 160 1 777.6 0 1 161 4 777.6 0 4 162 8 777.6 0 8 163 3 777.6 0 3 165 2 777.6 0 2 166 5 777.6 0 5 167 2 777.6 0 2 168 5 777.6 0 5 169 8 777.6 0 8 170 3 777.6 0 3 171 2 777.6 0 2 172 3 777.6 0 3 173 8 777.6 0 8 174 4 777.6 0 4 175 2 777.6 0 2 176 2 777.6 0 2 177 6 777.6 0 6 178 3 777.6 0 3 179 2 777.6 0 2 180 3 777.6 0 3 181 38 777.6 0 38 182 20 777.6 0 20 183 11 777.6 0 11 184 7 777.6 0 7 185 4 777.6 0 4 186 14 777.6 0 14 187 6 777.6 0 6 188 5 777.6 0 5 189 9 777.6 0 9 190 5 777.6 0 5 191 10 777.6 0 10 192 14 777.6 0 14 193 112 777.6 0 112 194 36 777.6 0 36 195 146 777.6 0 146 196 91 777.6 0 91 197 168 777.6 0 168 198 3 777.6 0 3 199 3 777.6 0 3 200 3 777.6 0 3 201 4 777.6 0 4 202 4 777.6 0 4 203 3 777.6 0 3 204 5 777.6 0 5 205 3 777.6 0 3 206 6 777.6 0 6 207 1 777.6 0 1 208 4 777.6 0 4 209 3 777.6 0 3 210 1 777.6 0 1 211 4 777.6 0 4 212 8 777.6 0 8 213 15 777.6 0 15 214 16 777.6 0 16 215 22 777.6 0 22 216 11 777.6 0 11 217 23 777.6 0 23 218 32 777.6 0 32 219 36 777.6 0 36 220 7 777.6 0 7 221 3 777.6 0 3 222 2 777.6 0 2 223 6 777.6 0 6 224 6 777.6 0 6 225 7 777.6 0 7 226 9 777.6 0 9 227 11 777.6 0 11 228 8 777.6 0 8 229 9 777.6 0 9 230 20 777.6 0 20 231 7 777.6 0 7 232 3 777.6 0 3 233 3 777.6 0 3 234 7 777.6 0 7 235 4 777.6 0 4 236 9 777.6 0 9 237 5 777.6 0 5 238 25 777.6 0 25 239 16 777.6 0 16 240 20 777.6 0 20 241 4 777.6 0 4 243 9 777.6 0 9 244 11 777.6 0 11 245 33 777.6 0 33 246 9 777.6 0 9 247 7 777.6 0 7 248 11 777.6 0 11 249 3 777.6 0 3 250 2 777.6 0 2 251 21 777.6 0 21 252 2 777.6 0 2 253 15 777.6 0 15 254 10 777.6 0 10 255 11 777.6 0 11 256 10 777.6 0 10 257 29 777.6 0 29 258 23 777.6 0 23 259 31 777.6 0 31 260 12 777.6 0 12 261 6 777.6 0 6 262 12 777.6 0 12 263 10 777.6 0 10 264 44 777.6 0 44 265 88 777.6 0 88 266 100 777.6 0 100 267 13 777.6 0 13 268 22 777.6 0 22 269 8 777.6 0 8 270 5 777.6 0 5 271 1 777.6 0 1 272 3 777.6 0 3 273 7 777.6 0 7 274 8 777.6 0 8 275 14 777.6 0 14 276 1 777.6 0 1 277 5 777.6 0 5 278 9 777.6 0 9 279 29 777.6 0 29 280 16 777.6 0 16 281 21 777.6 0 21 282 17 777.6 0 17 283 38 777.6 0 38 284 115 777.6 0 115 285 19 777.6 0 19 286 17 777.6 0 17 287 71 777.6 0 71 288 62 777.6 0 62 289 273 777.6 0 273 290 34 777.6 0 34 291 9 777.6 0 9 292 16 777.6 0 16 293 104 777.6 0 104 294 16 777.6 0 16 295 14 777.6 0 14 296 36 777.6 0 36 297 28 777.6 0 28 298 28 777.6 0 28 299 18 777.6 0 18 300 17 777.6 0 17 301 31 777.6 0 31 Finished in 0.18 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,698 Reads with adapters: 72 (1.9%) Reads that were too short: 4 (0.1%) Reads written (passing filters): 68 (1.8%) Total basepairs processed: 764,818 bp Total written (filtered): 17,590 bp (2.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 72 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 5 19 2 0.0 3 2 20 52 0.0 3 52 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (147 us/read; 0.41 M reads/minute). === Summary === Total reads processed: 68 Reads with adapters: 4 (5.9%) Reads written (passing filters): 68 (100.0%) Total basepairs processed: 17,590 bp Total written (filtered): 17,578 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 1.1 0 4