This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 103.21 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 151,601 (4.8%) Reads that were too short: 86,120 (2.7%) Reads written (passing filters): 65,481 (2.1%) Total basepairs processed: 958,672,960 bp Total written (filtered): 17,964,190 bp (1.9%) === Adapter 1 === Sequence: AACGCG; Type: regular 5'; Length: 6; Trimmed: 151601 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 53740 777.6 0 53740 7 150 777.6 0 150 8 1196 777.6 0 1196 9 44 777.6 0 44 10 21 777.6 0 21 11 27 777.6 0 27 12 18 777.6 0 18 13 11 777.6 0 11 14 13 777.6 0 13 15 9 777.6 0 9 16 24 777.6 0 24 17 12 777.6 0 12 18 18 777.6 0 18 19 14 777.6 0 14 20 14 777.6 0 14 21 10 777.6 0 10 22 14 777.6 0 14 23 14 777.6 0 14 24 8 777.6 0 8 25 12 777.6 0 12 26 6 777.6 0 6 27 3 777.6 0 3 28 19 777.6 0 19 29 10 777.6 0 10 30 21 777.6 0 21 31 15 777.6 0 15 32 4 777.6 0 4 33 106 777.6 0 106 34 16 777.6 0 16 35 10 777.6 0 10 36 9 777.6 0 9 37 8 777.6 0 8 38 5 777.6 0 5 39 23 777.6 0 23 40 14 777.6 0 14 41 17 777.6 0 17 42 14 777.6 0 14 43 16 777.6 0 16 44 12 777.6 0 12 45 17 777.6 0 17 46 56 777.6 0 56 47 27 777.6 0 27 48 15 777.6 0 15 49 7 777.6 0 7 50 13 777.6 0 13 51 13 777.6 0 13 52 15 777.6 0 15 53 15 777.6 0 15 54 9 777.6 0 9 55 20 777.6 0 20 56 19 777.6 0 19 57 8 777.6 0 8 58 22 777.6 0 22 59 12 777.6 0 12 60 7 777.6 0 7 61 15 777.6 0 15 62 15 777.6 0 15 63 5 777.6 0 5 64 8 777.6 0 8 65 7 777.6 0 7 66 5 777.6 0 5 67 13 777.6 0 13 68 17 777.6 0 17 69 35 777.6 0 35 70 19 777.6 0 19 71 236 777.6 0 236 72 279 777.6 0 279 73 236 777.6 0 236 74 20 777.6 0 20 75 24 777.6 0 24 76 16 777.6 0 16 77 55 777.6 0 55 78 32 777.6 0 32 79 32 777.6 0 32 80 27 777.6 0 27 81 80 777.6 0 80 82 61 777.6 0 61 83 81 777.6 0 81 84 141 777.6 0 141 85 37 777.6 0 37 86 27 777.6 0 27 87 19 777.6 0 19 88 31 777.6 0 31 89 21 777.6 0 21 90 45 777.6 0 45 91 38 777.6 0 38 92 57 777.6 0 57 93 166 777.6 0 166 94 75 777.6 0 75 95 19 777.6 0 19 96 14 777.6 0 14 97 20 777.6 0 20 98 7 777.6 0 7 99 37 777.6 0 37 100 50 777.6 0 50 101 25 777.6 0 25 102 15 777.6 0 15 103 15 777.6 0 15 104 17 777.6 0 17 105 19 777.6 0 19 106 25 777.6 0 25 107 13 777.6 0 13 108 11 777.6 0 11 109 20 777.6 0 20 110 32 777.6 0 32 111 29 777.6 0 29 112 38 777.6 0 38 113 30 777.6 0 30 114 37 777.6 0 37 115 31 777.6 0 31 116 26 777.6 0 26 117 88 777.6 0 88 118 55 777.6 0 55 119 59 777.6 0 59 120 78 777.6 0 78 121 36 777.6 0 36 122 75 777.6 0 75 123 109 777.6 0 109 124 112 777.6 0 112 125 146 777.6 0 146 126 41 777.6 0 41 127 240 777.6 0 240 128 106 777.6 0 106 129 117 777.6 0 117 130 38 777.6 0 38 131 56 777.6 0 56 132 37 777.6 0 37 133 39 777.6 0 39 134 76 777.6 0 76 135 64 777.6 0 64 136 49 777.6 0 49 137 55 777.6 0 55 138 53 777.6 0 53 139 44 777.6 0 44 140 17 777.6 0 17 141 31 777.6 0 31 142 18 777.6 0 18 143 33 777.6 0 33 144 52 777.6 0 52 145 64 777.6 0 64 146 36 777.6 0 36 147 24 777.6 0 24 148 9 777.6 0 9 149 35 777.6 0 35 150 28 777.6 0 28 151 34 777.6 0 34 152 26 777.6 0 26 153 37 777.6 0 37 154 67 777.6 0 67 155 38 777.6 0 38 156 32 777.6 0 32 157 54 777.6 0 54 158 61 777.6 0 61 159 38 777.6 0 38 160 32 777.6 0 32 161 35 777.6 0 35 162 45 777.6 0 45 163 48 777.6 0 48 164 76 777.6 0 76 165 56 777.6 0 56 166 44 777.6 0 44 167 38 777.6 0 38 168 49 777.6 0 49 169 28 777.6 0 28 170 43 777.6 0 43 171 43 777.6 0 43 172 67 777.6 0 67 173 63 777.6 0 63 174 34 777.6 0 34 175 15 777.6 0 15 176 28 777.6 0 28 177 53 777.6 0 53 178 345 777.6 0 345 179 156 777.6 0 156 180 105 777.6 0 105 181 68 777.6 0 68 182 283 777.6 0 283 183 161 777.6 0 161 184 265 777.6 0 265 185 42 777.6 0 42 186 38 777.6 0 38 187 20 777.6 0 20 188 222 777.6 0 222 189 254 777.6 0 254 190 164 777.6 0 164 191 119 777.6 0 119 192 68 777.6 0 68 193 23 777.6 0 23 194 21 777.6 0 21 195 18 777.6 0 18 196 106 777.6 0 106 197 333 777.6 0 333 198 783 777.6 0 783 199 54 777.6 0 54 200 11 777.6 0 11 201 41 777.6 0 41 202 59 777.6 0 59 203 179 777.6 0 179 204 127 777.6 0 127 205 35 777.6 0 35 206 37 777.6 0 37 207 32 777.6 0 32 208 37 777.6 0 37 209 59 777.6 0 59 210 107 777.6 0 107 211 80 777.6 0 80 212 63 777.6 0 63 213 58 777.6 0 58 214 63 777.6 0 63 215 101 777.6 0 101 216 71 777.6 0 71 217 188 777.6 0 188 218 208 777.6 0 208 219 439 777.6 0 439 220 720 777.6 0 720 221 517 777.6 0 517 222 960 777.6 0 960 223 1006 777.6 0 1006 224 396 777.6 0 396 225 297 777.6 0 297 226 314 777.6 0 314 227 82 777.6 0 82 228 103 777.6 0 103 229 50 777.6 0 50 230 69 777.6 0 69 231 101 777.6 0 101 232 57 777.6 0 57 233 178 777.6 0 178 234 294 777.6 0 294 235 364 777.6 0 364 236 962 777.6 0 962 237 165 777.6 0 165 238 558 777.6 0 558 239 138 777.6 0 138 240 128 777.6 0 128 241 203 777.6 0 203 242 176 777.6 0 176 243 543 777.6 0 543 244 5982 777.6 0 5982 245 4732 777.6 0 4732 246 8116 777.6 0 8116 247 628 777.6 0 628 248 1039 777.6 0 1039 249 200 777.6 0 200 250 214 777.6 0 214 251 134 777.6 0 134 252 152 777.6 0 152 253 177 777.6 0 177 254 567 777.6 0 567 255 213 777.6 0 213 256 130 777.6 0 130 257 29 777.6 0 29 258 19 777.6 0 19 259 50 777.6 0 50 260 32 777.6 0 32 261 234 777.6 0 234 262 116 777.6 0 116 263 423 777.6 0 423 264 128 777.6 0 128 265 127 777.6 0 127 266 109 777.6 0 109 267 232 777.6 0 232 268 199 777.6 0 199 269 136 777.6 0 136 270 128 777.6 0 128 271 185 777.6 0 185 272 150 777.6 0 150 273 67 777.6 0 67 274 48 777.6 0 48 275 148 777.6 0 148 276 102 777.6 0 102 277 134 777.6 0 134 278 213 777.6 0 213 279 88 777.6 0 88 280 35 777.6 0 35 281 65 777.6 0 65 282 71 777.6 0 71 283 312 777.6 0 312 284 351 777.6 0 351 285 247 777.6 0 247 286 308 777.6 0 308 287 446 777.6 0 446 288 6468 777.6 0 6468 289 17781 777.6 0 17781 290 20032 777.6 0 20032 291 3912 777.6 0 3912 292 65 777.6 0 65 293 75 777.6 0 75 294 45 777.6 0 45 295 41 777.6 0 41 296 61 777.6 0 61 297 35 777.6 0 35 298 15 777.6 0 15 299 18 777.6 0 18 300 19 777.6 0 19 301 13 777.6 0 13 Finished in 3.32 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 65,481 Reads with adapters: 53,791 (82.1%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 53,784 (82.1%) Total basepairs processed: 17,964,190 bp Total written (filtered): 14,736,111 bp (82.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 53791 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 0 1 1 19 10 0.0 3 0 0 7 3 20 368 0.0 4 2 346 12 6 2 21 53188 0.0 4 52044 1014 100 22 8 22 148 0.0 4 33 113 0 1 1 23 17 0.0 4 3 0 12 1 1 24 39 0.0 4 0 0 0 36 3 25 4 0.0 4 0 0 0 0 4 28 1 0.0 4 0 1 30 1 0.0 4 0 0 0 1 35 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 128 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.27 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 53,784 Reads with adapters: 1,675 (3.1%) Reads written (passing filters): 53,784 (100.0%) Total basepairs processed: 14,736,111 bp Total written (filtered): 14,653,697 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1675 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.1% C: 18.5% G: 74.7% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 20 840.4 0 20 4 9 210.1 0 9 5 1 52.5 0 1 9 3 0.2 0 0 3 10 4 0.1 1 0 4 11 1225 0.0 1 6 1219 12 110 0.0 1 3 107 15 3 0.0 1 0 3 16 1 0.0 1 0 1 152 16 0.0 1 8 8 164 1 0.0 1 0 1 210 5 0.0 1 3 2 224 5 0.0 1 5 225 230 0.0 1 218 12 226 2 0.0 1 1 1 234 1 0.0 1 0 1 261 30 0.0 1 20 10 262 9 0.0 1 9