This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.92 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 30,509 (1.0%) Reads that were too short: 10,701 (0.3%) Reads written (passing filters): 19,808 (0.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 4,509,196 bp (0.5%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 30509 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1091 777.6 0 1091 7 49 777.6 0 49 8 36 777.6 0 36 9 11 777.6 0 11 10 10 777.6 0 10 11 6 777.6 0 6 12 5 777.6 0 5 13 12 777.6 0 12 14 4 777.6 0 4 15 8 777.6 0 8 16 14 777.6 0 14 17 10 777.6 0 10 18 10 777.6 0 10 19 17 777.6 0 17 20 6 777.6 0 6 21 9 777.6 0 9 22 6 777.6 0 6 23 8 777.6 0 8 24 8 777.6 0 8 25 12 777.6 0 12 26 6 777.6 0 6 27 12 777.6 0 12 28 9 777.6 0 9 29 6 777.6 0 6 30 11 777.6 0 11 31 9 777.6 0 9 32 11 777.6 0 11 33 15 777.6 0 15 34 7 777.6 0 7 35 6 777.6 0 6 36 7 777.6 0 7 37 9 777.6 0 9 38 14 777.6 0 14 39 16 777.6 0 16 40 7 777.6 0 7 41 7 777.6 0 7 42 13 777.6 0 13 43 12 777.6 0 12 44 8 777.6 0 8 45 12 777.6 0 12 46 11 777.6 0 11 47 16 777.6 0 16 48 58 777.6 0 58 49 16 777.6 0 16 50 17 777.6 0 17 51 5 777.6 0 5 52 13 777.6 0 13 53 23 777.6 0 23 54 14 777.6 0 14 55 13 777.6 0 13 56 27 777.6 0 27 57 30 777.6 0 30 58 26 777.6 0 26 59 53 777.6 0 53 60 38 777.6 0 38 61 18 777.6 0 18 62 3165 777.6 0 3165 63 34 777.6 0 34 64 8907 777.6 0 8907 65 132 777.6 0 132 66 28 777.6 0 28 67 31 777.6 0 31 68 19 777.6 0 19 69 15 777.6 0 15 70 36 777.6 0 36 71 26 777.6 0 26 72 40 777.6 0 40 73 656 777.6 0 656 74 393 777.6 0 393 75 445 777.6 0 445 76 25 777.6 0 25 77 177 777.6 0 177 78 303 777.6 0 303 79 271 777.6 0 271 80 9 777.6 0 9 81 1 777.6 0 1 82 9 777.6 0 9 83 6 777.6 0 6 84 8 777.6 0 8 85 4 777.6 0 4 86 6 777.6 0 6 87 7 777.6 0 7 88 7 777.6 0 7 89 9 777.6 0 9 90 4 777.6 0 4 91 8 777.6 0 8 92 7 777.6 0 7 93 12 777.6 0 12 94 5 777.6 0 5 95 10 777.6 0 10 96 5 777.6 0 5 97 15 777.6 0 15 98 13 777.6 0 13 99 5 777.6 0 5 100 9 777.6 0 9 101 11 777.6 0 11 102 4 777.6 0 4 103 9 777.6 0 9 104 9 777.6 0 9 105 3 777.6 0 3 106 12 777.6 0 12 107 65 777.6 0 65 108 62 777.6 0 62 109 12 777.6 0 12 110 6 777.6 0 6 111 5 777.6 0 5 112 9 777.6 0 9 113 75 777.6 0 75 114 22 777.6 0 22 115 9 777.6 0 9 116 47 777.6 0 47 117 4 777.6 0 4 118 9 777.6 0 9 119 10 777.6 0 10 120 17 777.6 0 17 121 19 777.6 0 19 122 11 777.6 0 11 123 17 777.6 0 17 124 18 777.6 0 18 125 22 777.6 0 22 126 12 777.6 0 12 127 156 777.6 0 156 128 131 777.6 0 131 129 77 777.6 0 77 130 139 777.6 0 139 131 12 777.6 0 12 132 6 777.6 0 6 133 18 777.6 0 18 134 39 777.6 0 39 135 38 777.6 0 38 136 196 777.6 0 196 137 206 777.6 0 206 138 14 777.6 0 14 139 15 777.6 0 15 140 14 777.6 0 14 141 5 777.6 0 5 142 8 777.6 0 8 143 9 777.6 0 9 144 8 777.6 0 8 145 33 777.6 0 33 146 322 777.6 0 322 147 88 777.6 0 88 148 16 777.6 0 16 149 29 777.6 0 29 150 43 777.6 0 43 151 9 777.6 0 9 152 10 777.6 0 10 153 5 777.6 0 5 154 7 777.6 0 7 155 16 777.6 0 16 156 14 777.6 0 14 157 107 777.6 0 107 158 9 777.6 0 9 159 15 777.6 0 15 160 18 777.6 0 18 161 19 777.6 0 19 162 12 777.6 0 12 163 6 777.6 0 6 164 25 777.6 0 25 165 52 777.6 0 52 166 72 777.6 0 72 167 17 777.6 0 17 168 13 777.6 0 13 169 15 777.6 0 15 170 8 777.6 0 8 171 7 777.6 0 7 172 6 777.6 0 6 173 7 777.6 0 7 174 6 777.6 0 6 175 7 777.6 0 7 176 6 777.6 0 6 177 8 777.6 0 8 178 4 777.6 0 4 179 2 777.6 0 2 180 11 777.6 0 11 181 10 777.6 0 10 182 7 777.6 0 7 183 5 777.6 0 5 184 81 777.6 0 81 185 48 777.6 0 48 186 60 777.6 0 60 187 20 777.6 0 20 188 6 777.6 0 6 189 2 777.6 0 2 190 5 777.6 0 5 191 2 777.6 0 2 192 11 777.6 0 11 193 8 777.6 0 8 194 7 777.6 0 7 195 16 777.6 0 16 196 42 777.6 0 42 197 48 777.6 0 48 198 4 777.6 0 4 199 7 777.6 0 7 200 7 777.6 0 7 201 9 777.6 0 9 202 7 777.6 0 7 203 5 777.6 0 5 204 16 777.6 0 16 205 10 777.6 0 10 206 20 777.6 0 20 207 5 777.6 0 5 208 10 777.6 0 10 209 18 777.6 0 18 210 5 777.6 0 5 211 6 777.6 0 6 212 16 777.6 0 16 213 10 777.6 0 10 214 6 777.6 0 6 215 34 777.6 0 34 216 32 777.6 0 32 217 89 777.6 0 89 218 15 777.6 0 15 219 36 777.6 0 36 220 12 777.6 0 12 221 15 777.6 0 15 222 6 777.6 0 6 223 11 777.6 0 11 224 26 777.6 0 26 225 13 777.6 0 13 226 33 777.6 0 33 227 22 777.6 0 22 228 14 777.6 0 14 229 14 777.6 0 14 230 13 777.6 0 13 231 28 777.6 0 28 232 41 777.6 0 41 233 69 777.6 0 69 234 94 777.6 0 94 235 171 777.6 0 171 236 3065 777.6 0 3065 237 3707 777.6 0 3707 238 2070 777.6 0 2070 239 16 777.6 0 16 240 4 777.6 0 4 241 3 777.6 0 3 242 4 777.6 0 4 243 5 777.6 0 5 244 11 777.6 0 11 245 12 777.6 0 12 246 15 777.6 0 15 247 12 777.6 0 12 248 76 777.6 0 76 249 2 777.6 0 2 250 8 777.6 0 8 251 8 777.6 0 8 252 71 777.6 0 71 253 12 777.6 0 12 254 20 777.6 0 20 255 22 777.6 0 22 256 14 777.6 0 14 257 5 777.6 0 5 258 3 777.6 0 3 259 4 777.6 0 4 260 9 777.6 0 9 261 5 777.6 0 5 262 2 777.6 0 2 263 4 777.6 0 4 264 8 777.6 0 8 265 9 777.6 0 9 266 10 777.6 0 10 267 9 777.6 0 9 268 2 777.6 0 2 269 7 777.6 0 7 270 7 777.6 0 7 271 3 777.6 0 3 272 10 777.6 0 10 273 6 777.6 0 6 274 10 777.6 0 10 275 2 777.6 0 2 276 4 777.6 0 4 277 18 777.6 0 18 278 21 777.6 0 21 279 9 777.6 0 9 280 34 777.6 0 34 281 50 777.6 0 50 282 115 777.6 0 115 283 63 777.6 0 63 284 27 777.6 0 27 285 26 777.6 0 26 286 20 777.6 0 20 287 4 777.6 0 4 288 8 777.6 0 8 289 3 777.6 0 3 290 2 777.6 0 2 291 3 777.6 0 3 292 2 777.6 0 2 293 11 777.6 0 11 294 1 777.6 0 1 295 7 777.6 0 7 296 6 777.6 0 6 297 14 777.6 0 14 298 11 777.6 0 11 299 6 777.6 0 6 300 14 777.6 0 14 301 28 777.6 0 28 Finished in 0.89 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 19,808 Reads with adapters: 27 (0.1%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 24 (0.1%) Total basepairs processed: 4,509,196 bp Total written (filtered): 3,210 bp (0.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 27 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 23 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 5 0.0 3 0 0 0 5 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (417 us/read; 0.14 M reads/minute). === Summary === Total reads processed: 24 Reads with adapters: 1 (4.2%) Reads written (passing filters): 24 (100.0%) Total basepairs processed: 3,210 bp Total written (filtered): 3,207 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 0.4 0 1