This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 101.67 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 54,920 (1.7%) Reads that were too short: 45,175 (1.4%) Reads written (passing filters): 9,745 (0.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 1,672,698 bp (0.2%) === Adapter 1 === Sequence: CCGCTG; Type: regular 5'; Length: 6; Trimmed: 54920 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 18 777.6 0 18 7 12 777.6 0 12 8 50 777.6 0 50 9 109 777.6 0 109 10 37 777.6 0 37 11 18 777.6 0 18 12 12 777.6 0 12 13 30 777.6 0 30 14 13 777.6 0 13 15 13 777.6 0 13 16 10 777.6 0 10 17 10 777.6 0 10 18 18 777.6 0 18 19 26 777.6 0 26 20 17 777.6 0 17 21 10 777.6 0 10 22 21 777.6 0 21 23 6 777.6 0 6 24 11 777.6 0 11 25 14 777.6 0 14 26 27 777.6 0 27 27 13 777.6 0 13 28 6 777.6 0 6 29 17 777.6 0 17 30 18 777.6 0 18 31 13 777.6 0 13 32 17 777.6 0 17 33 13 777.6 0 13 34 15 777.6 0 15 35 9 777.6 0 9 36 11 777.6 0 11 37 19 777.6 0 19 38 12 777.6 0 12 39 8 777.6 0 8 40 19 777.6 0 19 41 11 777.6 0 11 42 53 777.6 0 53 43 32 777.6 0 32 44 9 777.6 0 9 45 9 777.6 0 9 46 14 777.6 0 14 47 13 777.6 0 13 48 12 777.6 0 12 49 27 777.6 0 27 50 15 777.6 0 15 51 9 777.6 0 9 52 8 777.6 0 8 53 8 777.6 0 8 54 9 777.6 0 9 55 7 777.6 0 7 56 9 777.6 0 9 57 16 777.6 0 16 58 16 777.6 0 16 59 13 777.6 0 13 60 13 777.6 0 13 61 13 777.6 0 13 62 9 777.6 0 9 63 8 777.6 0 8 64 8 777.6 0 8 65 9 777.6 0 9 66 6 777.6 0 6 67 12 777.6 0 12 68 5 777.6 0 5 69 14 777.6 0 14 70 8 777.6 0 8 71 5 777.6 0 5 72 18 777.6 0 18 73 16 777.6 0 16 74 27 777.6 0 27 75 21 777.6 0 21 76 7 777.6 0 7 77 13 777.6 0 13 78 8 777.6 0 8 79 19 777.6 0 19 80 14 777.6 0 14 81 15 777.6 0 15 82 52 777.6 0 52 83 29 777.6 0 29 84 14 777.6 0 14 85 17 777.6 0 17 86 12 777.6 0 12 87 14 777.6 0 14 88 13 777.6 0 13 89 11 777.6 0 11 90 11 777.6 0 11 91 35 777.6 0 35 92 42 777.6 0 42 93 48 777.6 0 48 94 894 777.6 0 894 95 457 777.6 0 457 96 549 777.6 0 549 97 45 777.6 0 45 98 20 777.6 0 20 99 58 777.6 0 58 100 12 777.6 0 12 101 14 777.6 0 14 102 5 777.6 0 5 103 5 777.6 0 5 104 13 777.6 0 13 105 20 777.6 0 20 106 127 777.6 0 127 107 200 777.6 0 200 108 13 777.6 0 13 109 8 777.6 0 8 110 14 777.6 0 14 111 16 777.6 0 16 112 22 777.6 0 22 113 22 777.6 0 22 114 23 777.6 0 23 115 14 777.6 0 14 116 10 777.6 0 10 117 7 777.6 0 7 118 14 777.6 0 14 119 8 777.6 0 8 120 8 777.6 0 8 121 21 777.6 0 21 122 13 777.6 0 13 123 16 777.6 0 16 124 12 777.6 0 12 125 17 777.6 0 17 126 23 777.6 0 23 127 21 777.6 0 21 128 330 777.6 0 330 129 308 777.6 0 308 130 183 777.6 0 183 131 8 777.6 0 8 132 22 777.6 0 22 133 17 777.6 0 17 134 15 777.6 0 15 135 11 777.6 0 11 136 11 777.6 0 11 137 11 777.6 0 11 138 7 777.6 0 7 139 13 777.6 0 13 140 20 777.6 0 20 141 134 777.6 0 134 142 13 777.6 0 13 143 31 777.6 0 31 144 12 777.6 0 12 145 327 777.6 0 327 146 342 777.6 0 342 147 277 777.6 0 277 148 16 777.6 0 16 149 6 777.6 0 6 150 8 777.6 0 8 151 7 777.6 0 7 152 24 777.6 0 24 153 21 777.6 0 21 154 13 777.6 0 13 155 10 777.6 0 10 156 15 777.6 0 15 157 18 777.6 0 18 158 13 777.6 0 13 159 15 777.6 0 15 160 14 777.6 0 14 161 13 777.6 0 13 162 20 777.6 0 20 163 18 777.6 0 18 164 10 777.6 0 10 165 13 777.6 0 13 166 29 777.6 0 29 167 19 777.6 0 19 168 28 777.6 0 28 169 16 777.6 0 16 170 15 777.6 0 15 171 27 777.6 0 27 172 42 777.6 0 42 173 72 777.6 0 72 174 151 777.6 0 151 175 42 777.6 0 42 176 51 777.6 0 51 177 129 777.6 0 129 178 191 777.6 0 191 179 238 777.6 0 238 180 154 777.6 0 154 181 86 777.6 0 86 182 93 777.6 0 93 183 53 777.6 0 53 184 63 777.6 0 63 185 24 777.6 0 24 186 14 777.6 0 14 187 14 777.6 0 14 188 8 777.6 0 8 189 14 777.6 0 14 190 10 777.6 0 10 191 84 777.6 0 84 192 103 777.6 0 103 193 154 777.6 0 154 194 71 777.6 0 71 195 35 777.6 0 35 196 42 777.6 0 42 197 383 777.6 0 383 198 196 777.6 0 196 199 427 777.6 0 427 200 63 777.6 0 63 201 9 777.6 0 9 202 20 777.6 0 20 203 43 777.6 0 43 204 61 777.6 0 61 205 16 777.6 0 16 206 4 777.6 0 4 207 5 777.6 0 5 208 11 777.6 0 11 209 13 777.6 0 13 210 11 777.6 0 11 211 19 777.6 0 19 212 18 777.6 0 18 213 37 777.6 0 37 214 211 777.6 0 211 215 1013 777.6 0 1013 216 238 777.6 0 238 217 195 777.6 0 195 218 10 777.6 0 10 219 12 777.6 0 12 220 17 777.6 0 17 221 12 777.6 0 12 222 8 777.6 0 8 223 12 777.6 0 12 224 12 777.6 0 12 225 23 777.6 0 23 226 14 777.6 0 14 227 18 777.6 0 18 228 9 777.6 0 9 229 7 777.6 0 7 230 9 777.6 0 9 231 9 777.6 0 9 232 19 777.6 0 19 233 18 777.6 0 18 234 13 777.6 0 13 235 14 777.6 0 14 236 7 777.6 0 7 237 14 777.6 0 14 238 14 777.6 0 14 239 6 777.6 0 6 240 9 777.6 0 9 241 8 777.6 0 8 242 9 777.6 0 9 243 16 777.6 0 16 244 15 777.6 0 15 245 15 777.6 0 15 246 39 777.6 0 39 247 35 777.6 0 35 248 192 777.6 0 192 249 34 777.6 0 34 250 98 777.6 0 98 251 38 777.6 0 38 252 43 777.6 0 43 253 94 777.6 0 94 254 45 777.6 0 45 255 172 777.6 0 172 256 116 777.6 0 116 257 141 777.6 0 141 258 30 777.6 0 30 259 14 777.6 0 14 260 6 777.6 0 6 261 12 777.6 0 12 262 24 777.6 0 24 263 18 777.6 0 18 264 24 777.6 0 24 265 166 777.6 0 166 266 96 777.6 0 96 267 46 777.6 0 46 268 87 777.6 0 87 269 57 777.6 0 57 270 28 777.6 0 28 271 121 777.6 0 121 272 45 777.6 0 45 273 96 777.6 0 96 274 28 777.6 0 28 275 50 777.6 0 50 276 44 777.6 0 44 277 82 777.6 0 82 278 220 777.6 0 220 279 431 777.6 0 431 280 152 777.6 0 152 281 108 777.6 0 108 282 53 777.6 0 53 283 45 777.6 0 45 284 80 777.6 0 80 285 107 777.6 0 107 286 368 777.6 0 368 287 495 777.6 0 495 288 971 777.6 0 971 289 2990 777.6 0 2990 290 5229 777.6 0 5229 291 4012 777.6 0 4012 292 3827 777.6 0 3827 293 6463 777.6 0 6463 294 5399 777.6 0 5399 295 4403 777.6 0 4403 296 3370 777.6 0 3370 297 1546 777.6 0 1546 298 500 777.6 0 500 299 13 777.6 0 13 300 5 777.6 0 5 301 3 777.6 0 3 Finished in 0.46 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 9,745 Reads with adapters: 9 (0.1%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 7 (0.1%) Total basepairs processed: 1,672,698 bp Total written (filtered): 1,788 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 21 6 0.0 4 2 3 1 30 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (1429 us/read; 0.04 M reads/minute). === Summary === Total reads processed: 7 Reads with adapters: 1 (14.3%) Reads written (passing filters): 7 (100.0%) Total basepairs processed: 1,788 bp Total written (filtered): 1,777 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 11 1 0.0 1 0 1