This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.40 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 220,360 (6.9%) Reads that were too short: 132,878 (4.2%) Reads written (passing filters): 87,482 (2.7%) Total basepairs processed: 958,672,960 bp Total written (filtered): 13,861,426 bp (1.4%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 220360 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2234 777.6 0 2234 7 63 777.6 0 63 8 3 777.6 0 3 9 67 777.6 0 67 10 4 777.6 0 4 11 4 777.6 0 4 12 17 777.6 0 17 13 6 777.6 0 6 14 3 777.6 0 3 15 2 777.6 0 2 16 2 777.6 0 2 17 7 777.6 0 7 18 4 777.6 0 4 19 4 777.6 0 4 20 3 777.6 0 3 21 5 777.6 0 5 22 3 777.6 0 3 23 2 777.6 0 2 24 5 777.6 0 5 25 4 777.6 0 4 26 1 777.6 0 1 27 4 777.6 0 4 28 4 777.6 0 4 29 5 777.6 0 5 30 4 777.6 0 4 31 5 777.6 0 5 32 5 777.6 0 5 33 3 777.6 0 3 34 7 777.6 0 7 36 3 777.6 0 3 37 3 777.6 0 3 38 10 777.6 0 10 39 9 777.6 0 9 40 3 777.6 0 3 41 80 777.6 0 80 42 34 777.6 0 34 43 3 777.6 0 3 44 1 777.6 0 1 45 5 777.6 0 5 46 5 777.6 0 5 47 1 777.6 0 1 48 5 777.6 0 5 49 1 777.6 0 1 50 3 777.6 0 3 52 2 777.6 0 2 53 2 777.6 0 2 54 4 777.6 0 4 55 9 777.6 0 9 56 3 777.6 0 3 57 5 777.6 0 5 58 17 777.6 0 17 59 24 777.6 0 24 60 6 777.6 0 6 61 2 777.6 0 2 62 3 777.6 0 3 63 6 777.6 0 6 64 3 777.6 0 3 65 2 777.6 0 2 66 4 777.6 0 4 67 8 777.6 0 8 68 1 777.6 0 1 69 15 777.6 0 15 70 21 777.6 0 21 71 11 777.6 0 11 72 38 777.6 0 38 73 5 777.6 0 5 74 1 777.6 0 1 75 6 777.6 0 6 76 5 777.6 0 5 77 3 777.6 0 3 78 1 777.6 0 1 79 5 777.6 0 5 80 26 777.6 0 26 81 332 777.6 0 332 82 440 777.6 0 440 83 510 777.6 0 510 84 162 777.6 0 162 85 467 777.6 0 467 86 677 777.6 0 677 87 767 777.6 0 767 88 118 777.6 0 118 89 403 777.6 0 403 90 64 777.6 0 64 91 97 777.6 0 97 92 585 777.6 0 585 93 536 777.6 0 536 94 177 777.6 0 177 95 99 777.6 0 99 96 164 777.6 0 164 97 585 777.6 0 585 98 824 777.6 0 824 99 702 777.6 0 702 100 195 777.6 0 195 101 170 777.6 0 170 102 396 777.6 0 396 103 1487 777.6 0 1487 104 517 777.6 0 517 105 241 777.6 0 241 106 405 777.6 0 405 107 164 777.6 0 164 108 1131 777.6 0 1131 109 1212 777.6 0 1212 110 704 777.6 0 704 111 372 777.6 0 372 112 433 777.6 0 433 113 709 777.6 0 709 114 294 777.6 0 294 115 1066 777.6 0 1066 116 313 777.6 0 313 117 304 777.6 0 304 118 1370 777.6 0 1370 119 395 777.6 0 395 120 506 777.6 0 506 121 497 777.6 0 497 122 431 777.6 0 431 123 460 777.6 0 460 124 524 777.6 0 524 125 826 777.6 0 826 126 470 777.6 0 470 127 777 777.6 0 777 128 629 777.6 0 629 129 583 777.6 0 583 130 603 777.6 0 603 131 292 777.6 0 292 132 661 777.6 0 661 133 1082 777.6 0 1082 134 1004 777.6 0 1004 135 1076 777.6 0 1076 136 735 777.6 0 735 137 813 777.6 0 813 138 1363 777.6 0 1363 139 1273 777.6 0 1273 140 718 777.6 0 718 141 763 777.6 0 763 142 412 777.6 0 412 143 705 777.6 0 705 144 1764 777.6 0 1764 145 582 777.6 0 582 146 915 777.6 0 915 147 881 777.6 0 881 148 721 777.6 0 721 149 961 777.6 0 961 150 695 777.6 0 695 151 2040 777.6 0 2040 152 772 777.6 0 772 153 1062 777.6 0 1062 154 1097 777.6 0 1097 155 575 777.6 0 575 156 2226 777.6 0 2226 157 1123 777.6 0 1123 158 735 777.6 0 735 159 1173 777.6 0 1173 160 584 777.6 0 584 161 903 777.6 0 903 162 536 777.6 0 536 163 639 777.6 0 639 164 1111 777.6 0 1111 165 609 777.6 0 609 166 823 777.6 0 823 167 449 777.6 0 449 168 549 777.6 0 549 169 311 777.6 0 311 170 577 777.6 0 577 171 671 777.6 0 671 172 1305 777.6 0 1305 173 806 777.6 0 806 174 325 777.6 0 325 175 278 777.6 0 278 176 449 777.6 0 449 177 876 777.6 0 876 178 577 777.6 0 577 179 1189 777.6 0 1189 180 1665 777.6 0 1665 181 717 777.6 0 717 182 830 777.6 0 830 183 497 777.6 0 497 184 680 777.6 0 680 185 652 777.6 0 652 186 571 777.6 0 571 187 571 777.6 0 571 188 1068 777.6 0 1068 189 1129 777.6 0 1129 190 1578 777.6 0 1578 191 563 777.6 0 563 192 417 777.6 0 417 193 254 777.6 0 254 194 581 777.6 0 581 195 592 777.6 0 592 196 735 777.6 0 735 197 876 777.6 0 876 198 283 777.6 0 283 199 475 777.6 0 475 200 888 777.6 0 888 201 1297 777.6 0 1297 202 1129 777.6 0 1129 203 605 777.6 0 605 204 394 777.6 0 394 205 559 777.6 0 559 206 1165 777.6 0 1165 207 812 777.6 0 812 208 928 777.6 0 928 209 1171 777.6 0 1171 210 1189 777.6 0 1189 211 8369 777.6 0 8369 212 4789 777.6 0 4789 213 9978 777.6 0 9978 214 3166 777.6 0 3166 215 817 777.6 0 817 216 645 777.6 0 645 217 651 777.6 0 651 218 703 777.6 0 703 219 818 777.6 0 818 220 1749 777.6 0 1749 221 664 777.6 0 664 222 935 777.6 0 935 223 995 777.6 0 995 224 726 777.6 0 726 225 880 777.6 0 880 226 978 777.6 0 978 227 566 777.6 0 566 228 529 777.6 0 529 229 480 777.6 0 480 230 510 777.6 0 510 231 697 777.6 0 697 232 747 777.6 0 747 233 770 777.6 0 770 234 979 777.6 0 979 235 569 777.6 0 569 236 547 777.6 0 547 237 623 777.6 0 623 238 685 777.6 0 685 239 861 777.6 0 861 240 1231 777.6 0 1231 241 1349 777.6 0 1349 242 896 777.6 0 896 243 851 777.6 0 851 244 660 777.6 0 660 245 788 777.6 0 788 246 583 777.6 0 583 247 600 777.6 0 600 248 310 777.6 0 310 249 786 777.6 0 786 250 1075 777.6 0 1075 251 1045 777.6 0 1045 252 604 777.6 0 604 253 431 777.6 0 431 254 766 777.6 0 766 255 832 777.6 0 832 256 507 777.6 0 507 257 464 777.6 0 464 258 443 777.6 0 443 259 795 777.6 0 795 260 1195 777.6 0 1195 261 823 777.6 0 823 262 968 777.6 0 968 263 625 777.6 0 625 264 580 777.6 0 580 265 835 777.6 0 835 266 612 777.6 0 612 267 783 777.6 0 783 268 570 777.6 0 570 269 747 777.6 0 747 270 2218 777.6 0 2218 271 1907 777.6 0 1907 272 5907 777.6 0 5907 273 796 777.6 0 796 274 686 777.6 0 686 275 637 777.6 0 637 276 818 777.6 0 818 277 1089 777.6 0 1089 278 1279 777.6 0 1279 279 925 777.6 0 925 280 745 777.6 0 745 281 446 777.6 0 446 282 873 777.6 0 873 283 1045 777.6 0 1045 284 633 777.6 0 633 285 946 777.6 0 946 286 709 777.6 0 709 287 929 777.6 0 929 288 840 777.6 0 840 289 985 777.6 0 985 290 950 777.6 0 950 291 932 777.6 0 932 292 973 777.6 0 973 293 2840 777.6 0 2840 294 19399 777.6 0 19399 295 1513 777.6 0 1513 296 944 777.6 0 944 297 936 777.6 0 936 298 1034 777.6 0 1034 299 1093 777.6 0 1093 300 1063 777.6 0 1063 301 656 777.6 0 656 Finished in 3.23 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 87,482 Reads with adapters: 22 (0.0%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 15 (0.0%) Total basepairs processed: 13,861,426 bp Total written (filtered): 2,243 bp (0.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 3 22 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 66 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (667 us/read; 0.09 M reads/minute). === Summary === Total reads processed: 15 Reads with adapters: 1 (6.7%) Reads written (passing filters): 15 (100.0%) Total basepairs processed: 2,243 bp Total written (filtered): 2,240 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 0.2 0 1