This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGTGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 106.53 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 257,880 (8.1%) Reads that were too short: 5,213 (0.2%) Reads written (passing filters): 252,667 (7.9%) Total basepairs processed: 958,672,960 bp Total written (filtered): 70,608,212 bp (7.4%) === Adapter 1 === Sequence: AGTGTT; Type: regular 5'; Length: 6; Trimmed: 257880 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1607 777.6 0 1607 7 3861 777.6 0 3861 8 211290 777.6 0 211290 9 182 777.6 0 182 10 24 777.6 0 24 11 11 777.6 0 11 12 14 777.6 0 14 13 13 777.6 0 13 14 26 777.6 0 26 15 12 777.6 0 12 16 15 777.6 0 15 17 8 777.6 0 8 18 8 777.6 0 8 19 19 777.6 0 19 20 21 777.6 0 21 21 23 777.6 0 23 22 21 777.6 0 21 23 10 777.6 0 10 24 14 777.6 0 14 25 17 777.6 0 17 26 13 777.6 0 13 27 19 777.6 0 19 28 41 777.6 0 41 29 42 777.6 0 42 30 189 777.6 0 189 31 26 777.6 0 26 32 1447 777.6 0 1447 33 31 777.6 0 31 34 17 777.6 0 17 35 22 777.6 0 22 36 18 777.6 0 18 37 14 777.6 0 14 38 9 777.6 0 9 39 15 777.6 0 15 40 16 777.6 0 16 41 54 777.6 0 54 42 11 777.6 0 11 43 9 777.6 0 9 44 17 777.6 0 17 45 7 777.6 0 7 46 7 777.6 0 7 47 8 777.6 0 8 48 17 777.6 0 17 49 18 777.6 0 18 50 28 777.6 0 28 51 48 777.6 0 48 52 16 777.6 0 16 53 26 777.6 0 26 54 17 777.6 0 17 55 25 777.6 0 25 56 44 777.6 0 44 57 26 777.6 0 26 58 36 777.6 0 36 59 20 777.6 0 20 60 20 777.6 0 20 61 32 777.6 0 32 62 301 777.6 0 301 63 160 777.6 0 160 64 146 777.6 0 146 65 78 777.6 0 78 66 156 777.6 0 156 67 155 777.6 0 155 68 4262 777.6 0 4262 69 1192 777.6 0 1192 70 6805 777.6 0 6805 71 46 777.6 0 46 72 51 777.6 0 51 73 323 777.6 0 323 74 22 777.6 0 22 75 12 777.6 0 12 76 25 777.6 0 25 77 63 777.6 0 63 78 68 777.6 0 68 79 96 777.6 0 96 80 52 777.6 0 52 81 71 777.6 0 71 82 19 777.6 0 19 83 27 777.6 0 27 84 44 777.6 0 44 85 15 777.6 0 15 86 39 777.6 0 39 87 55 777.6 0 55 88 69 777.6 0 69 89 36 777.6 0 36 90 45 777.6 0 45 91 21 777.6 0 21 92 22 777.6 0 22 93 68 777.6 0 68 94 143 777.6 0 143 95 137 777.6 0 137 96 108 777.6 0 108 97 37 777.6 0 37 98 46 777.6 0 46 99 1148 777.6 0 1148 100 1406 777.6 0 1406 101 2998 777.6 0 2998 102 20 777.6 0 20 103 12 777.6 0 12 104 8 777.6 0 8 105 10 777.6 0 10 106 9 777.6 0 9 107 14 777.6 0 14 108 15 777.6 0 15 109 10 777.6 0 10 110 21 777.6 0 21 111 21 777.6 0 21 112 13 777.6 0 13 113 15 777.6 0 15 114 15 777.6 0 15 115 21 777.6 0 21 116 17 777.6 0 17 117 20 777.6 0 20 118 45 777.6 0 45 119 32 777.6 0 32 120 21 777.6 0 21 121 29 777.6 0 29 122 19 777.6 0 19 123 17 777.6 0 17 124 38 777.6 0 38 125 39 777.6 0 39 126 38 777.6 0 38 127 48 777.6 0 48 128 51 777.6 0 51 129 80 777.6 0 80 130 86 777.6 0 86 131 276 777.6 0 276 132 1611 777.6 0 1611 133 265 777.6 0 265 134 35 777.6 0 35 135 61 777.6 0 61 136 125 777.6 0 125 137 30 777.6 0 30 138 35 777.6 0 35 139 36 777.6 0 36 140 33 777.6 0 33 141 48 777.6 0 48 142 47 777.6 0 47 143 66 777.6 0 66 144 77 777.6 0 77 145 3062 777.6 0 3062 146 318 777.6 0 318 147 298 777.6 0 298 148 8 777.6 0 8 149 25 777.6 0 25 150 13 777.6 0 13 151 7 777.6 0 7 152 5 777.6 0 5 153 22 777.6 0 22 154 18 777.6 0 18 155 22 777.6 0 22 156 23 777.6 0 23 157 16 777.6 0 16 158 21 777.6 0 21 159 25 777.6 0 25 160 25 777.6 0 25 161 18 777.6 0 18 162 30 777.6 0 30 163 21 777.6 0 21 164 30 777.6 0 30 165 25 777.6 0 25 166 27 777.6 0 27 167 112 777.6 0 112 168 67 777.6 0 67 169 60 777.6 0 60 170 18 777.6 0 18 171 12 777.6 0 12 172 15 777.6 0 15 173 13 777.6 0 13 174 36 777.6 0 36 175 28 777.6 0 28 176 22 777.6 0 22 177 28 777.6 0 28 178 20 777.6 0 20 179 21 777.6 0 21 180 30 777.6 0 30 181 32 777.6 0 32 182 30 777.6 0 30 183 34 777.6 0 34 184 59 777.6 0 59 185 54 777.6 0 54 186 25 777.6 0 25 187 25 777.6 0 25 188 35 777.6 0 35 189 23 777.6 0 23 190 46 777.6 0 46 191 98 777.6 0 98 192 134 777.6 0 134 193 201 777.6 0 201 194 161 777.6 0 161 195 345 777.6 0 345 196 286 777.6 0 286 197 474 777.6 0 474 198 852 777.6 0 852 199 884 777.6 0 884 200 555 777.6 0 555 201 76 777.6 0 76 202 29 777.6 0 29 203 52 777.6 0 52 204 74 777.6 0 74 205 54 777.6 0 54 206 68 777.6 0 68 207 44 777.6 0 44 208 28 777.6 0 28 209 32 777.6 0 32 210 37 777.6 0 37 211 43 777.6 0 43 212 100 777.6 0 100 213 94 777.6 0 94 214 58 777.6 0 58 215 39 777.6 0 39 216 71 777.6 0 71 217 36 777.6 0 36 218 42 777.6 0 42 219 150 777.6 0 150 220 81 777.6 0 81 221 240 777.6 0 240 222 46 777.6 0 46 223 56 777.6 0 56 224 48 777.6 0 48 225 44 777.6 0 44 226 33 777.6 0 33 227 27 777.6 0 27 228 25 777.6 0 25 229 22 777.6 0 22 230 13 777.6 0 13 231 14 777.6 0 14 232 17 777.6 0 17 233 20 777.6 0 20 234 36 777.6 0 36 235 38 777.6 0 38 236 42 777.6 0 42 237 22 777.6 0 22 238 31 777.6 0 31 239 30 777.6 0 30 240 30 777.6 0 30 241 40 777.6 0 40 242 43 777.6 0 43 243 50 777.6 0 50 244 40 777.6 0 40 245 26 777.6 0 26 246 32 777.6 0 32 247 24 777.6 0 24 248 53 777.6 0 53 249 20 777.6 0 20 250 38 777.6 0 38 251 28 777.6 0 28 252 63 777.6 0 63 253 45 777.6 0 45 254 46 777.6 0 46 255 22 777.6 0 22 256 14 777.6 0 14 257 23 777.6 0 23 258 21 777.6 0 21 259 20 777.6 0 20 260 34 777.6 0 34 261 44 777.6 0 44 262 22 777.6 0 22 263 32 777.6 0 32 264 39 777.6 0 39 265 95 777.6 0 95 266 93 777.6 0 93 267 76 777.6 0 76 268 47 777.6 0 47 269 58 777.6 0 58 270 15 777.6 0 15 271 16 777.6 0 16 272 26 777.6 0 26 273 35 777.6 0 35 274 21 777.6 0 21 275 24 777.6 0 24 276 16 777.6 0 16 277 16 777.6 0 16 278 21 777.6 0 21 279 38 777.6 0 38 280 23 777.6 0 23 281 11 777.6 0 11 282 23 777.6 0 23 283 25 777.6 0 25 284 47 777.6 0 47 285 46 777.6 0 46 286 31 777.6 0 31 287 29 777.6 0 29 288 49 777.6 0 49 289 26 777.6 0 26 290 28 777.6 0 28 291 35 777.6 0 35 292 36 777.6 0 36 293 30 777.6 0 30 294 23 777.6 0 23 295 8 777.6 0 8 296 10 777.6 0 10 297 32 777.6 0 32 298 66 777.6 0 66 299 34 777.6 0 34 300 53 777.6 0 53 301 1166 777.6 0 1166 Finished in 12.70 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 252,667 Reads with adapters: 216,581 (85.7%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 216,571 (85.7%) Total basepairs processed: 70,608,212 bp Total written (filtered): 59,130,335 bp (83.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 216581 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 0 0 0 2 17 7 0.0 3 0 0 0 6 1 18 98 0.0 3 0 0 81 10 7 19 1874 0.0 3 0 1608 134 87 45 20 213582 0.0 4 205592 6935 792 188 75 21 677 0.0 4 89 555 19 6 8 22 68 0.0 4 6 2 53 1 6 23 237 0.0 4 3 1 0 182 51 24 9 0.0 4 1 1 0 5 2 25 2 0.0 4 1 0 0 1 27 2 0.0 4 2 28 2 0.0 4 0 1 0 1 35 1 0.0 4 0 1 36 1 0.0 4 1 39 2 0.0 4 1 1 41 1 0.0 4 0 1 46 2 0.0 4 2 53 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.38 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 216,571 Reads with adapters: 21,584 (10.0%) Reads written (passing filters): 216,571 (100.0%) Total basepairs processed: 59,130,335 bp Total written (filtered): 58,881,778 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 21584 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 2.5% G: 93.8% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 3383.9 0 6 4 12 846.0 0 12 8 2 3.3 0 2 9 15 0.8 0 0 15 10 247 0.2 1 6 241 11 21189 0.1 1 73 21116 12 24 0.0 1 1 23 13 4 0.0 1 0 4 14 16 0.0 1 0 16 15 1 0.0 1 0 1 38 1 0.0 1 1 151 37 0.0 1 8 29 153 2 0.0 1 0 2 170 2 0.0 1 2 190 1 0.0 1 1 209 6 0.0 1 4 2 224 11 0.0 1 11 225 2 0.0 1 2 226 1 0.0 1 1 269 1 0.0 1 1 272 4 0.0 1 3 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.