This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 105.73 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 372,969 (11.7%) Reads that were too short: 125,889 (4.0%) Reads written (passing filters): 247,080 (7.8%) Total basepairs processed: 958,672,960 bp Total written (filtered): 68,978,830 bp (7.2%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 372969 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2555 777.6 0 2555 7 4785 777.6 0 4785 8 208899 777.6 0 208899 9 225 777.6 0 225 10 26 777.6 0 26 11 20 777.6 0 20 12 20 777.6 0 20 13 27 777.6 0 27 14 20 777.6 0 20 15 25 777.6 0 25 16 21 777.6 0 21 17 21 777.6 0 21 18 287 777.6 0 287 19 29 777.6 0 29 20 26 777.6 0 26 21 35 777.6 0 35 22 14 777.6 0 14 23 13 777.6 0 13 24 29 777.6 0 29 25 10 777.6 0 10 26 12 777.6 0 12 27 16 777.6 0 16 28 15 777.6 0 15 29 18 777.6 0 18 30 23 777.6 0 23 31 31 777.6 0 31 32 17 777.6 0 17 33 20 777.6 0 20 34 27 777.6 0 27 35 5409 777.6 0 5409 36 47 777.6 0 47 37 36 777.6 0 36 38 31 777.6 0 31 39 24 777.6 0 24 40 24 777.6 0 24 41 23 777.6 0 23 42 27 777.6 0 27 43 19 777.6 0 19 44 17 777.6 0 17 45 93 777.6 0 93 46 597 777.6 0 597 47 68 777.6 0 68 48 1432 777.6 0 1432 49 33 777.6 0 33 50 23 777.6 0 23 51 28 777.6 0 28 52 95 777.6 0 95 53 19 777.6 0 19 54 23 777.6 0 23 55 56 777.6 0 56 56 24 777.6 0 24 57 32 777.6 0 32 58 31 777.6 0 31 59 15 777.6 0 15 60 14 777.6 0 14 61 20 777.6 0 20 62 20 777.6 0 20 63 14 777.6 0 14 64 25 777.6 0 25 65 18 777.6 0 18 66 20 777.6 0 20 67 18 777.6 0 18 68 11 777.6 0 11 69 27 777.6 0 27 70 22 777.6 0 22 71 20 777.6 0 20 72 28 777.6 0 28 73 38 777.6 0 38 74 32 777.6 0 32 75 42 777.6 0 42 76 28 777.6 0 28 77 26 777.6 0 26 78 38 777.6 0 38 79 29 777.6 0 29 80 16 777.6 0 16 81 27 777.6 0 27 82 36 777.6 0 36 83 25 777.6 0 25 84 51 777.6 0 51 85 43 777.6 0 43 86 52 777.6 0 52 87 25 777.6 0 25 88 57 777.6 0 57 89 81 777.6 0 81 90 50 777.6 0 50 91 98 777.6 0 98 92 50 777.6 0 50 93 34 777.6 0 34 94 125 777.6 0 125 95 122 777.6 0 122 96 184 777.6 0 184 97 196 777.6 0 196 98 107 777.6 0 107 99 338 777.6 0 338 100 58 777.6 0 58 101 42 777.6 0 42 102 59 777.6 0 59 103 25 777.6 0 25 104 37 777.6 0 37 105 100 777.6 0 100 106 150 777.6 0 150 107 34 777.6 0 34 108 44 777.6 0 44 109 217 777.6 0 217 110 42 777.6 0 42 111 29 777.6 0 29 112 33 777.6 0 33 113 40 777.6 0 40 114 43 777.6 0 43 115 43 777.6 0 43 116 94 777.6 0 94 117 106 777.6 0 106 118 101 777.6 0 101 119 117 777.6 0 117 120 146 777.6 0 146 121 96 777.6 0 96 122 69 777.6 0 69 123 54 777.6 0 54 124 82 777.6 0 82 125 68 777.6 0 68 126 79 777.6 0 79 127 70 777.6 0 70 128 63 777.6 0 63 129 77 777.6 0 77 130 129 777.6 0 129 131 97 777.6 0 97 132 43 777.6 0 43 133 97 777.6 0 97 134 131 777.6 0 131 135 226 777.6 0 226 136 231 777.6 0 231 137 135 777.6 0 135 138 180 777.6 0 180 139 104 777.6 0 104 140 149 777.6 0 149 141 64 777.6 0 64 142 38 777.6 0 38 143 52 777.6 0 52 144 153 777.6 0 153 145 656 777.6 0 656 146 460 777.6 0 460 147 1241 777.6 0 1241 148 81 777.6 0 81 149 62 777.6 0 62 150 47 777.6 0 47 151 67 777.6 0 67 152 102 777.6 0 102 153 116 777.6 0 116 154 71 777.6 0 71 155 128 777.6 0 128 156 129 777.6 0 129 157 432 777.6 0 432 158 124 777.6 0 124 159 153 777.6 0 153 160 100 777.6 0 100 161 87 777.6 0 87 162 93 777.6 0 93 163 99 777.6 0 99 164 154 777.6 0 154 165 357 777.6 0 357 166 192 777.6 0 192 167 175 777.6 0 175 168 110 777.6 0 110 169 130 777.6 0 130 170 57 777.6 0 57 171 74 777.6 0 74 172 163 777.6 0 163 173 3029 777.6 0 3029 174 515 777.6 0 515 175 433 777.6 0 433 176 239 777.6 0 239 177 50 777.6 0 50 178 75 777.6 0 75 179 131 777.6 0 131 180 180 777.6 0 180 181 361 777.6 0 361 182 685 777.6 0 685 183 540 777.6 0 540 184 443 777.6 0 443 185 161 777.6 0 161 186 317 777.6 0 317 187 188 777.6 0 188 188 368 777.6 0 368 189 116 777.6 0 116 190 107 777.6 0 107 191 203 777.6 0 203 192 238 777.6 0 238 193 419 777.6 0 419 194 154 777.6 0 154 195 47 777.6 0 47 196 57 777.6 0 57 197 112 777.6 0 112 198 188 777.6 0 188 199 124 777.6 0 124 200 85 777.6 0 85 201 109 777.6 0 109 202 129 777.6 0 129 203 284 777.6 0 284 204 195 777.6 0 195 205 206 777.6 0 206 206 226 777.6 0 226 207 118 777.6 0 118 208 145 777.6 0 145 209 181 777.6 0 181 210 164 777.6 0 164 211 246 777.6 0 246 212 468 777.6 0 468 213 262 777.6 0 262 214 287 777.6 0 287 215 349 777.6 0 349 216 266 777.6 0 266 217 267 777.6 0 267 218 341 777.6 0 341 219 378 777.6 0 378 220 290 777.6 0 290 221 240 777.6 0 240 222 318 777.6 0 318 223 476 777.6 0 476 224 529 777.6 0 529 225 8309 777.6 0 8309 226 3703 777.6 0 3703 227 7966 777.6 0 7966 228 609 777.6 0 609 229 1303 777.6 0 1303 230 903 777.6 0 903 231 805 777.6 0 805 232 780 777.6 0 780 233 487 777.6 0 487 234 662 777.6 0 662 235 433 777.6 0 433 236 160 777.6 0 160 237 195 777.6 0 195 238 148 777.6 0 148 239 115 777.6 0 115 240 112 777.6 0 112 241 147 777.6 0 147 242 204 777.6 0 204 243 178 777.6 0 178 244 196 777.6 0 196 245 227 777.6 0 227 246 630 777.6 0 630 247 423 777.6 0 423 248 567 777.6 0 567 249 113 777.6 0 113 250 2042 777.6 0 2042 251 2448 777.6 0 2448 252 3392 777.6 0 3392 253 364 777.6 0 364 254 299 777.6 0 299 255 308 777.6 0 308 256 374 777.6 0 374 257 174 777.6 0 174 258 145 777.6 0 145 259 78 777.6 0 78 260 125 777.6 0 125 261 129 777.6 0 129 262 147 777.6 0 147 263 151 777.6 0 151 264 178 777.6 0 178 265 513 777.6 0 513 266 407 777.6 0 407 267 308 777.6 0 308 268 169 777.6 0 169 269 128 777.6 0 128 270 153 777.6 0 153 271 914 777.6 0 914 272 560 777.6 0 560 273 628 777.6 0 628 274 407 777.6 0 407 275 1253 777.6 0 1253 276 289 777.6 0 289 277 217 777.6 0 217 278 319 777.6 0 319 279 663 777.6 0 663 280 735 777.6 0 735 281 608 777.6 0 608 282 600 777.6 0 600 283 470 777.6 0 470 284 776 777.6 0 776 285 1473 777.6 0 1473 286 750 777.6 0 750 287 659 777.6 0 659 288 404 777.6 0 404 289 491 777.6 0 491 290 1116 777.6 0 1116 291 923 777.6 0 923 292 998 777.6 0 998 293 362 777.6 0 362 294 396 777.6 0 396 295 560 777.6 0 560 296 827 777.6 0 827 297 1728 777.6 0 1728 298 2436 777.6 0 2436 299 4308 777.6 0 4308 300 7492 777.6 0 7492 301 44755 777.6 0 44755 Finished in 11.51 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 247,080 Reads with adapters: 215,878 (87.4%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 215,862 (87.4%) Total basepairs processed: 68,978,830 bp Total written (filtered): 59,579,439 bp (86.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 215878 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 53 0.0 3 0 0 46 7 16 1432 0.0 3 0 1396 32 4 17 213083 0.0 3 209743 3211 107 22 18 427 0.0 3 32 377 13 5 19 688 0.0 3 0 0 645 43 20 122 0.0 3 1 0 0 121 24 1 0.0 3 1 25 2 0.0 3 1 0 1 26 2 0.0 3 1 0 0 1 30 1 0.0 3 1 33 1 0.0 3 1 34 1 0.0 3 1 36 1 0.0 3 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 1 45 1 0.0 3 0 0 0 1 46 6 0.0 3 0 0 0 6 49 11 0.0 3 0 0 0 11 53 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 94 4 0.0 3 0 0 0 4 95 1 0.0 3 0 0 0 1 97 4 0.0 3 0 0 0 4 109 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 135 7 0.0 3 0 0 2 5 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.73 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 215,862 Reads with adapters: 2,655 (1.2%) Reads written (passing filters): 215,862 (100.0%) Total basepairs processed: 59,579,439 bp Total written (filtered): 59,571,441 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2655 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.8% C: 42.9% G: 40.5% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2622 3372.8 0 2622 4 33 843.2 0 33