This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.27 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 154,269 (4.8%) Reads that were too short: 96,854 (3.0%) Reads written (passing filters): 57,415 (1.8%) Total basepairs processed: 958,672,960 bp Total written (filtered): 12,623,571 bp (1.3%) === Adapter 1 === Sequence: AACCGA; Type: regular 5'; Length: 6; Trimmed: 154269 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 279 777.6 0 279 7 308 777.6 0 308 8 1086 777.6 0 1086 9 24257 777.6 0 24257 10 47 777.6 0 47 11 58 777.6 0 58 12 11 777.6 0 11 13 23 777.6 0 23 14 2 777.6 0 2 15 4 777.6 0 4 16 2 777.6 0 2 17 5 777.6 0 5 18 2 777.6 0 2 19 7 777.6 0 7 20 7 777.6 0 7 21 3 777.6 0 3 22 3 777.6 0 3 23 4 777.6 0 4 24 7 777.6 0 7 25 7 777.6 0 7 26 6 777.6 0 6 27 4 777.6 0 4 28 5 777.6 0 5 29 5 777.6 0 5 30 5 777.6 0 5 31 6 777.6 0 6 32 3 777.6 0 3 33 2 777.6 0 2 34 5 777.6 0 5 35 5 777.6 0 5 36 4 777.6 0 4 37 4 777.6 0 4 38 2 777.6 0 2 39 10 777.6 0 10 40 3 777.6 0 3 41 3 777.6 0 3 42 2 777.6 0 2 43 3 777.6 0 3 44 5 777.6 0 5 45 4 777.6 0 4 46 1 777.6 0 1 47 4 777.6 0 4 48 6 777.6 0 6 49 5 777.6 0 5 50 3 777.6 0 3 51 2 777.6 0 2 52 2 777.6 0 2 53 4 777.6 0 4 54 4 777.6 0 4 55 5 777.6 0 5 56 6 777.6 0 6 57 4 777.6 0 4 58 4 777.6 0 4 59 8 777.6 0 8 60 10 777.6 0 10 61 11 777.6 0 11 62 28 777.6 0 28 63 6 777.6 0 6 64 8 777.6 0 8 65 6 777.6 0 6 66 4 777.6 0 4 67 4 777.6 0 4 68 6 777.6 0 6 69 5 777.6 0 5 70 39 777.6 0 39 71 185 777.6 0 185 72 1107 777.6 0 1107 73 239 777.6 0 239 74 36 777.6 0 36 75 37 777.6 0 37 76 257 777.6 0 257 77 178 777.6 0 178 78 171 777.6 0 171 79 128 777.6 0 128 80 249 777.6 0 249 81 190 777.6 0 190 82 271 777.6 0 271 83 489 777.6 0 489 84 232 777.6 0 232 85 435 777.6 0 435 86 128 777.6 0 128 87 20 777.6 0 20 88 57 777.6 0 57 89 114 777.6 0 114 90 145 777.6 0 145 91 35 777.6 0 35 92 268 777.6 0 268 93 535 777.6 0 535 94 373 777.6 0 373 95 310 777.6 0 310 96 79 777.6 0 79 97 36 777.6 0 36 98 83 777.6 0 83 99 107 777.6 0 107 100 221 777.6 0 221 101 166 777.6 0 166 102 95 777.6 0 95 103 63 777.6 0 63 104 57 777.6 0 57 105 252 777.6 0 252 106 68 777.6 0 68 107 41 777.6 0 41 108 50 777.6 0 50 109 75 777.6 0 75 110 113 777.6 0 113 111 150 777.6 0 150 112 105 777.6 0 105 113 147 777.6 0 147 114 146 777.6 0 146 115 264 777.6 0 264 116 200 777.6 0 200 117 187 777.6 0 187 118 241 777.6 0 241 119 374 777.6 0 374 120 216 777.6 0 216 121 230 777.6 0 230 122 239 777.6 0 239 123 197 777.6 0 197 124 238 777.6 0 238 125 351 777.6 0 351 126 851 777.6 0 851 127 379 777.6 0 379 128 306 777.6 0 306 129 135 777.6 0 135 130 155 777.6 0 155 131 190 777.6 0 190 132 155 777.6 0 155 133 153 777.6 0 153 134 191 777.6 0 191 135 211 777.6 0 211 136 243 777.6 0 243 137 188 777.6 0 188 138 345 777.6 0 345 139 206 777.6 0 206 140 256 777.6 0 256 141 158 777.6 0 158 142 92 777.6 0 92 143 115 777.6 0 115 144 226 777.6 0 226 145 426 777.6 0 426 146 173 777.6 0 173 147 146 777.6 0 146 148 94 777.6 0 94 149 108 777.6 0 108 150 104 777.6 0 104 151 109 777.6 0 109 152 230 777.6 0 230 153 310 777.6 0 310 154 263 777.6 0 263 155 221 777.6 0 221 156 205 777.6 0 205 157 243 777.6 0 243 158 304 777.6 0 304 159 186 777.6 0 186 160 182 777.6 0 182 161 129 777.6 0 129 162 150 777.6 0 150 163 149 777.6 0 149 164 210 777.6 0 210 165 309 777.6 0 309 166 225 777.6 0 225 167 161 777.6 0 161 168 136 777.6 0 136 169 294 777.6 0 294 170 125 777.6 0 125 171 169 777.6 0 169 172 241 777.6 0 241 173 651 777.6 0 651 174 493 777.6 0 493 175 336 777.6 0 336 176 95 777.6 0 95 177 202 777.6 0 202 178 309 777.6 0 309 179 310 777.6 0 310 180 445 777.6 0 445 181 280 777.6 0 280 182 184 777.6 0 184 183 175 777.6 0 175 184 169 777.6 0 169 185 267 777.6 0 267 186 153 777.6 0 153 187 94 777.6 0 94 188 66 777.6 0 66 189 245 777.6 0 245 190 476 777.6 0 476 191 505 777.6 0 505 192 288 777.6 0 288 193 227 777.6 0 227 194 118 777.6 0 118 195 147 777.6 0 147 196 253 777.6 0 253 197 2202 777.6 0 2202 198 723 777.6 0 723 199 157 777.6 0 157 200 135 777.6 0 135 201 180 777.6 0 180 202 4112 777.6 0 4112 203 437 777.6 0 437 204 281 777.6 0 281 205 313 777.6 0 313 206 208 777.6 0 208 207 166 777.6 0 166 208 84 777.6 0 84 209 207 777.6 0 207 210 282 777.6 0 282 211 163 777.6 0 163 212 138 777.6 0 138 213 163 777.6 0 163 214 130 777.6 0 130 215 173 777.6 0 173 216 502 777.6 0 502 217 1046 777.6 0 1046 218 808 777.6 0 808 219 483 777.6 0 483 220 95 777.6 0 95 221 123 777.6 0 123 222 249 777.6 0 249 223 556 777.6 0 556 224 551 777.6 0 551 225 360 777.6 0 360 226 358 777.6 0 358 227 652 777.6 0 652 228 758 777.6 0 758 229 5765 777.6 0 5765 230 7415 777.6 0 7415 231 2709 777.6 0 2709 232 106 777.6 0 106 233 162 777.6 0 162 234 216 777.6 0 216 235 267 777.6 0 267 236 104 777.6 0 104 237 101 777.6 0 101 238 111 777.6 0 111 239 140 777.6 0 140 240 105 777.6 0 105 241 117 777.6 0 117 242 128 777.6 0 128 243 154 777.6 0 154 244 363 777.6 0 363 245 1234 777.6 0 1234 246 312 777.6 0 312 247 250 777.6 0 250 248 92 777.6 0 92 249 53 777.6 0 53 250 79 777.6 0 79 251 198 777.6 0 198 252 282 777.6 0 282 253 336 777.6 0 336 254 369 777.6 0 369 255 425 777.6 0 425 256 239 777.6 0 239 257 117 777.6 0 117 258 211 777.6 0 211 259 853 777.6 0 853 260 7636 777.6 0 7636 261 1921 777.6 0 1921 262 207 777.6 0 207 263 295 777.6 0 295 264 385 777.6 0 385 265 378 777.6 0 378 266 948 777.6 0 948 267 341 777.6 0 341 268 350 777.6 0 350 269 203 777.6 0 203 270 264 777.6 0 264 271 429 777.6 0 429 272 268 777.6 0 268 273 331 777.6 0 331 274 217 777.6 0 217 275 185 777.6 0 185 276 243 777.6 0 243 277 379 777.6 0 379 278 606 777.6 0 606 279 650 777.6 0 650 280 506 777.6 0 506 281 217 777.6 0 217 282 319 777.6 0 319 283 486 777.6 0 486 284 656 777.6 0 656 285 774 777.6 0 774 286 740 777.6 0 740 287 1247 777.6 0 1247 288 937 777.6 0 937 289 845 777.6 0 845 290 405 777.6 0 405 291 480 777.6 0 480 292 990 777.6 0 990 293 18146 777.6 0 18146 294 8439 777.6 0 8439 295 8086 777.6 0 8086 296 177 777.6 0 177 297 115 777.6 0 115 298 154 777.6 0 154 299 136 777.6 0 136 300 161 777.6 0 161 301 191 777.6 0 191 Finished in 2.74 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 57,415 Reads with adapters: 25,875 (45.1%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 25,852 (45.0%) Total basepairs processed: 12,623,571 bp Total written (filtered): 7,033,060 bp (55.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 25875 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 19 0.0 3 0 0 14 5 19 253 0.0 3 0 229 11 6 7 20 25475 0.0 4 24594 718 109 41 13 21 61 0.0 4 8 48 3 1 1 22 10 0.0 4 0 0 5 0 5 23 24 0.0 4 0 0 0 22 2 24 2 0.0 4 1 0 0 0 1 25 1 0.0 4 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 1 46 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 68 2 0.0 4 0 0 0 0 2 69 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 1 104 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 115 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 215 1 0.0 4 1 222 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.10 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 25,852 Reads with adapters: 1,267 (4.9%) Reads written (passing filters): 25,852 (100.0%) Total basepairs processed: 7,033,060 bp Total written (filtered): 7,000,621 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1267 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.6% C: 2.1% G: 85.3% T: 3.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 403.9 0 1 4 1 101.0 0 1 6 1 6.3 0 1 9 124 0.1 0 11 113 10 570 0.0 1 17 553 11 462 0.0 1 1 461 12 1 0.0 1 0 1 13 6 0.0 1 0 6 26 1 0.0 1 0 1 150 7 0.0 1 4 3 153 1 0.0 1 1 208 86 0.0 1 69 17 209 3 0.0 1 3 210 1 0.0 1 1 223 1 0.0 1 1 261 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.