This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 104.21 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 220,926 (6.9%) Reads that were too short: 25,942 (0.8%) Reads written (passing filters): 194,984 (6.1%) Total basepairs processed: 958,672,960 bp Total written (filtered): 37,274,465 bp (3.9%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 220926 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1010 777.6 0 1010 7 231 777.6 0 231 8 659 777.6 0 659 9 25113 777.6 0 25113 10 67 777.6 0 67 11 22 777.6 0 22 12 28 777.6 0 28 13 19 777.6 0 19 14 15 777.6 0 15 15 18 777.6 0 18 16 26 777.6 0 26 17 27 777.6 0 27 18 23 777.6 0 23 19 19 777.6 0 19 20 27 777.6 0 27 21 38 777.6 0 38 22 31 777.6 0 31 23 37 777.6 0 37 24 60 777.6 0 60 25 107 777.6 0 107 26 335 777.6 0 335 27 24 777.6 0 24 28 34 777.6 0 34 29 33 777.6 0 33 30 39 777.6 0 39 31 35 777.6 0 35 32 28 777.6 0 28 33 20 777.6 0 20 34 16 777.6 0 16 35 23 777.6 0 23 36 24 777.6 0 24 37 21 777.6 0 21 38 18 777.6 0 18 39 20 777.6 0 20 40 23 777.6 0 23 41 16 777.6 0 16 42 20 777.6 0 20 43 18 777.6 0 18 44 20 777.6 0 20 45 20 777.6 0 20 46 22 777.6 0 22 47 30 777.6 0 30 48 30 777.6 0 30 49 27 777.6 0 27 50 21 777.6 0 21 51 25 777.6 0 25 52 19 777.6 0 19 53 42 777.6 0 42 54 50 777.6 0 50 55 50 777.6 0 50 56 28 777.6 0 28 57 27 777.6 0 27 58 61 777.6 0 61 59 62 777.6 0 62 60 35 777.6 0 35 61 126 777.6 0 126 62 107 777.6 0 107 63 252 777.6 0 252 64 20 777.6 0 20 65 61 777.6 0 61 66 25 777.6 0 25 67 19 777.6 0 19 68 28 777.6 0 28 69 37 777.6 0 37 70 77 777.6 0 77 71 56 777.6 0 56 72 21 777.6 0 21 73 24 777.6 0 24 74 25 777.6 0 25 75 14 777.6 0 14 76 16 777.6 0 16 77 25 777.6 0 25 78 209 777.6 0 209 79 72 777.6 0 72 80 189 777.6 0 189 81 80 777.6 0 80 82 498 777.6 0 498 83 383 777.6 0 383 84 247 777.6 0 247 85 213 777.6 0 213 86 98 777.6 0 98 87 73 777.6 0 73 88 184 777.6 0 184 89 68 777.6 0 68 90 91 777.6 0 91 91 48 777.6 0 48 92 87 777.6 0 87 93 149 777.6 0 149 94 196 777.6 0 196 95 190 777.6 0 190 96 294 777.6 0 294 97 333 777.6 0 333 98 617 777.6 0 617 99 1568 777.6 0 1568 100 611 777.6 0 611 101 578 777.6 0 578 102 1259 777.6 0 1259 103 925 777.6 0 925 104 495 777.6 0 495 105 1089 777.6 0 1089 106 863 777.6 0 863 107 1621 777.6 0 1621 108 442 777.6 0 442 109 1028 777.6 0 1028 110 1024 777.6 0 1024 111 1204 777.6 0 1204 112 18075 777.6 0 18075 113 11998 777.6 0 11998 114 72855 777.6 0 72855 115 374 777.6 0 374 116 139 777.6 0 139 117 133 777.6 0 133 118 392 777.6 0 392 119 578 777.6 0 578 120 894 777.6 0 894 121 829 777.6 0 829 122 1430 777.6 0 1430 123 136 777.6 0 136 124 133 777.6 0 133 125 1156 777.6 0 1156 126 588 777.6 0 588 127 303 777.6 0 303 128 124 777.6 0 124 129 109 777.6 0 109 130 135 777.6 0 135 131 157 777.6 0 157 132 160 777.6 0 160 133 131 777.6 0 131 134 165 777.6 0 165 135 145 777.6 0 145 136 190 777.6 0 190 137 198 777.6 0 198 138 187 777.6 0 187 139 247 777.6 0 247 140 119 777.6 0 119 141 135 777.6 0 135 142 233 777.6 0 233 143 149 777.6 0 149 144 237 777.6 0 237 145 188 777.6 0 188 146 187 777.6 0 187 147 196 777.6 0 196 148 319 777.6 0 319 149 299 777.6 0 299 150 217 777.6 0 217 151 85 777.6 0 85 152 183 777.6 0 183 153 175 777.6 0 175 154 284 777.6 0 284 155 161 777.6 0 161 156 141 777.6 0 141 157 228 777.6 0 228 158 184 777.6 0 184 159 207 777.6 0 207 160 182 777.6 0 182 161 190 777.6 0 190 162 166 777.6 0 166 163 257 777.6 0 257 164 214 777.6 0 214 165 392 777.6 0 392 166 414 777.6 0 414 167 446 777.6 0 446 168 453 777.6 0 453 169 254 777.6 0 254 170 554 777.6 0 554 171 1842 777.6 0 1842 172 1236 777.6 0 1236 173 1392 777.6 0 1392 174 832 777.6 0 832 175 160 777.6 0 160 176 232 777.6 0 232 177 183 777.6 0 183 178 282 777.6 0 282 179 333 777.6 0 333 180 416 777.6 0 416 181 329 777.6 0 329 182 314 777.6 0 314 183 2135 777.6 0 2135 184 1076 777.6 0 1076 185 1540 777.6 0 1540 186 221 777.6 0 221 187 162 777.6 0 162 188 200 777.6 0 200 189 196 777.6 0 196 190 369 777.6 0 369 191 7156 777.6 0 7156 192 3241 777.6 0 3241 193 1694 777.6 0 1694 194 450 777.6 0 450 195 213 777.6 0 213 196 228 777.6 0 228 197 225 777.6 0 225 198 345 777.6 0 345 199 1014 777.6 0 1014 200 573 777.6 0 573 201 133 777.6 0 133 202 128 777.6 0 128 203 203 777.6 0 203 204 277 777.6 0 277 205 157 777.6 0 157 206 156 777.6 0 156 207 143 777.6 0 143 208 107 777.6 0 107 209 210 777.6 0 210 210 181 777.6 0 181 211 254 777.6 0 254 212 205 777.6 0 205 213 182 777.6 0 182 214 178 777.6 0 178 215 284 777.6 0 284 216 234 777.6 0 234 217 249 777.6 0 249 218 220 777.6 0 220 219 174 777.6 0 174 220 280 777.6 0 280 221 86 777.6 0 86 222 116 777.6 0 116 223 282 777.6 0 282 224 271 777.6 0 271 225 219 777.6 0 219 226 214 777.6 0 214 227 226 777.6 0 226 228 322 777.6 0 322 229 271 777.6 0 271 230 307 777.6 0 307 231 170 777.6 0 170 232 170 777.6 0 170 233 134 777.6 0 134 234 189 777.6 0 189 235 347 777.6 0 347 236 194 777.6 0 194 237 174 777.6 0 174 238 117 777.6 0 117 239 129 777.6 0 129 240 141 777.6 0 141 241 137 777.6 0 137 242 132 777.6 0 132 243 481 777.6 0 481 244 419 777.6 0 419 245 210 777.6 0 210 246 165 777.6 0 165 247 174 777.6 0 174 248 82 777.6 0 82 249 86 777.6 0 86 250 123 777.6 0 123 251 586 777.6 0 586 252 717 777.6 0 717 253 407 777.6 0 407 254 271 777.6 0 271 255 371 777.6 0 371 256 244 777.6 0 244 257 310 777.6 0 310 258 138 777.6 0 138 259 96 777.6 0 96 260 117 777.6 0 117 261 144 777.6 0 144 262 201 777.6 0 201 263 273 777.6 0 273 264 246 777.6 0 246 265 224 777.6 0 224 266 161 777.6 0 161 267 348 777.6 0 348 268 794 777.6 0 794 269 624 777.6 0 624 270 171 777.6 0 171 271 179 777.6 0 179 272 190 777.6 0 190 273 209 777.6 0 209 274 186 777.6 0 186 275 163 777.6 0 163 276 117 777.6 0 117 277 199 777.6 0 199 278 216 777.6 0 216 279 233 777.6 0 233 280 376 777.6 0 376 281 164 777.6 0 164 282 287 777.6 0 287 283 316 777.6 0 316 284 317 777.6 0 317 285 235 777.6 0 235 286 187 777.6 0 187 287 161 777.6 0 161 288 114 777.6 0 114 289 175 777.6 0 175 290 248 777.6 0 248 291 311 777.6 0 311 292 391 777.6 0 391 293 606 777.6 0 606 294 265 777.6 0 265 295 169 777.6 0 169 296 668 777.6 0 668 297 1897 777.6 0 1897 298 377 777.6 0 377 299 234 777.6 0 234 300 331 777.6 0 331 301 68 777.6 0 68 Finished in 8.02 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 194,984 Reads with adapters: 26,891 (13.8%) Reads that were too short: 94 (0.0%) Reads written (passing filters): 26,797 (13.7%) Total basepairs processed: 37,274,465 bp Total written (filtered): 7,361,945 bp (19.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26891 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 9 0.0 3 0 0 8 1 16 174 0.0 3 0 170 4 17 25563 0.0 3 24951 590 16 6 18 94 0.0 3 7 80 5 2 19 18 0.0 3 3 4 6 5 20 876 0.0 3 821 13 0 42 21 2 0.0 3 1 1 22 2 0.0 3 0 0 0 2 23 3 0.0 3 1 0 0 2 24 6 0.0 3 1 0 0 5 25 1 0.0 3 0 1 26 3 0.0 3 1 0 0 2 30 1 0.0 3 0 0 0 1 39 1 0.0 3 1 51 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 68 2 0.0 3 0 0 0 2 71 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 79 6 0.0 3 0 0 0 6 80 1 0.0 3 0 0 0 1 82 9 0.0 3 0 0 0 9 84 2 0.0 3 0 0 0 2 101 8 0.0 3 0 0 0 8 102 7 0.0 3 0 0 0 7 108 4 0.0 3 0 0 0 4 109 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 8 0.0 3 0 0 0 8 120 1 0.0 3 0 0 0 1 124 1 0.0 3 1 139 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 181 58 0.0 3 0 0 1 57 182 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.20 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 26,797 Reads with adapters: 623 (2.3%) Reads written (passing filters): 26,797 (100.0%) Total basepairs processed: 7,361,945 bp Total written (filtered): 7,360,073 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 623 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 72.1% C: 4.2% G: 22.2% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 620 418.7 0 620 4 3 104.7 0 3