This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 105.59 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 21,104 (0.7%) Reads that were too short: 7,189 (0.2%) Reads written (passing filters): 13,915 (0.4%) Total basepairs processed: 958,672,960 bp Total written (filtered): 3,072,832 bp (0.3%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 21104 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 93 777.6 0 93 7 2237 777.6 0 2237 8 33 777.6 0 33 9 14 777.6 0 14 10 7 777.6 0 7 11 11 777.6 0 11 12 12 777.6 0 12 13 12 777.6 0 12 14 12 777.6 0 12 15 8 777.6 0 8 16 15 777.6 0 15 17 15 777.6 0 15 18 12 777.6 0 12 19 9 777.6 0 9 20 21 777.6 0 21 21 10 777.6 0 10 22 14 777.6 0 14 23 17 777.6 0 17 24 17 777.6 0 17 25 10 777.6 0 10 26 16 777.6 0 16 27 12 777.6 0 12 28 25 777.6 0 25 29 21 777.6 0 21 30 33 777.6 0 33 31 221 777.6 0 221 32 1046 777.6 0 1046 33 22 777.6 0 22 34 114 777.6 0 114 35 18 777.6 0 18 36 10 777.6 0 10 37 20 777.6 0 20 38 10 777.6 0 10 39 22 777.6 0 22 40 16 777.6 0 16 41 18 777.6 0 18 42 8 777.6 0 8 43 28 777.6 0 28 44 12 777.6 0 12 45 15 777.6 0 15 46 20 777.6 0 20 47 17 777.6 0 17 48 15 777.6 0 15 49 11 777.6 0 11 50 17 777.6 0 17 51 11 777.6 0 11 52 14 777.6 0 14 53 16 777.6 0 16 54 34 777.6 0 34 55 16 777.6 0 16 56 16 777.6 0 16 57 13 777.6 0 13 58 9 777.6 0 9 59 27 777.6 0 27 60 22 777.6 0 22 61 42 777.6 0 42 62 63 777.6 0 63 63 99 777.6 0 99 64 2016 777.6 0 2016 65 1074 777.6 0 1074 66 1029 777.6 0 1029 67 14 777.6 0 14 68 11 777.6 0 11 69 13 777.6 0 13 70 17 777.6 0 17 71 15 777.6 0 15 72 5 777.6 0 5 73 10 777.6 0 10 74 5 777.6 0 5 75 13 777.6 0 13 76 8 777.6 0 8 77 8 777.6 0 8 78 4 777.6 0 4 79 6 777.6 0 6 80 15 777.6 0 15 81 32 777.6 0 32 82 9 777.6 0 9 83 28 777.6 0 28 84 22 777.6 0 22 85 18 777.6 0 18 86 13 777.6 0 13 87 7 777.6 0 7 88 7 777.6 0 7 89 15 777.6 0 15 90 19 777.6 0 19 91 42 777.6 0 42 92 79 777.6 0 79 93 41 777.6 0 41 94 76 777.6 0 76 95 27 777.6 0 27 96 47 777.6 0 47 97 34 777.6 0 34 98 9 777.6 0 9 99 23 777.6 0 23 100 27 777.6 0 27 101 84 777.6 0 84 102 26 777.6 0 26 103 40 777.6 0 40 104 17 777.6 0 17 105 17 777.6 0 17 106 19 777.6 0 19 107 22 777.6 0 22 108 21 777.6 0 21 109 17 777.6 0 17 110 11 777.6 0 11 111 20 777.6 0 20 112 12 777.6 0 12 113 5 777.6 0 5 114 10 777.6 0 10 115 14 777.6 0 14 116 17 777.6 0 17 117 18 777.6 0 18 118 21 777.6 0 21 119 16 777.6 0 16 120 20 777.6 0 20 121 26 777.6 0 26 122 38 777.6 0 38 123 26 777.6 0 26 124 25 777.6 0 25 125 14 777.6 0 14 126 16 777.6 0 16 127 24 777.6 0 24 128 42 777.6 0 42 129 14 777.6 0 14 130 21 777.6 0 21 131 38 777.6 0 38 132 37 777.6 0 37 133 38 777.6 0 38 134 17 777.6 0 17 135 49 777.6 0 49 136 69 777.6 0 69 137 119 777.6 0 119 138 50 777.6 0 50 139 52 777.6 0 52 140 21 777.6 0 21 141 35 777.6 0 35 142 39 777.6 0 39 143 20 777.6 0 20 144 14 777.6 0 14 145 12 777.6 0 12 146 35 777.6 0 35 147 92 777.6 0 92 148 82 777.6 0 82 149 54 777.6 0 54 150 78 777.6 0 78 151 18 777.6 0 18 152 15 777.6 0 15 153 74 777.6 0 74 154 30 777.6 0 30 155 36 777.6 0 36 156 47 777.6 0 47 157 171 777.6 0 171 158 105 777.6 0 105 159 57 777.6 0 57 160 50 777.6 0 50 161 47 777.6 0 47 162 57 777.6 0 57 163 54 777.6 0 54 164 27 777.6 0 27 165 21 777.6 0 21 166 33 777.6 0 33 167 92 777.6 0 92 168 74 777.6 0 74 169 59 777.6 0 59 170 74 777.6 0 74 171 190 777.6 0 190 172 49 777.6 0 49 173 42 777.6 0 42 174 29 777.6 0 29 175 162 777.6 0 162 176 105 777.6 0 105 177 64 777.6 0 64 178 33 777.6 0 33 179 25 777.6 0 25 180 22 777.6 0 22 181 42 777.6 0 42 182 39 777.6 0 39 183 43 777.6 0 43 184 59 777.6 0 59 185 48 777.6 0 48 186 71 777.6 0 71 187 98 777.6 0 98 188 114 777.6 0 114 189 26 777.6 0 26 190 30 777.6 0 30 191 32 777.6 0 32 192 27 777.6 0 27 193 27 777.6 0 27 194 29 777.6 0 29 195 27 777.6 0 27 196 15 777.6 0 15 197 23 777.6 0 23 198 21 777.6 0 21 199 33 777.6 0 33 200 43 777.6 0 43 201 16 777.6 0 16 202 12 777.6 0 12 203 27 777.6 0 27 204 25 777.6 0 25 205 44 777.6 0 44 206 52 777.6 0 52 207 36 777.6 0 36 208 28 777.6 0 28 209 31 777.6 0 31 210 38 777.6 0 38 211 23 777.6 0 23 212 34 777.6 0 34 213 61 777.6 0 61 214 53 777.6 0 53 215 82 777.6 0 82 216 60 777.6 0 60 217 76 777.6 0 76 218 86 777.6 0 86 219 43 777.6 0 43 220 59 777.6 0 59 221 43 777.6 0 43 222 51 777.6 0 51 223 145 777.6 0 145 224 103 777.6 0 103 225 94 777.6 0 94 226 79 777.6 0 79 227 79 777.6 0 79 228 76 777.6 0 76 229 63 777.6 0 63 230 58 777.6 0 58 231 38 777.6 0 38 232 36 777.6 0 36 233 20 777.6 0 20 234 32 777.6 0 32 235 39 777.6 0 39 236 48 777.6 0 48 237 83 777.6 0 83 238 38 777.6 0 38 239 42 777.6 0 42 240 31 777.6 0 31 241 27 777.6 0 27 242 47 777.6 0 47 243 123 777.6 0 123 244 95 777.6 0 95 245 102 777.6 0 102 246 45 777.6 0 45 247 104 777.6 0 104 248 109 777.6 0 109 249 64 777.6 0 64 250 22 777.6 0 22 251 72 777.6 0 72 252 248 777.6 0 248 253 157 777.6 0 157 254 206 777.6 0 206 255 141 777.6 0 141 256 79 777.6 0 79 257 119 777.6 0 119 258 120 777.6 0 120 259 38 777.6 0 38 260 39 777.6 0 39 261 17 777.6 0 17 262 39 777.6 0 39 263 61 777.6 0 61 264 86 777.6 0 86 265 92 777.6 0 92 266 79 777.6 0 79 267 98 777.6 0 98 268 275 777.6 0 275 269 122 777.6 0 122 270 364 777.6 0 364 271 17 777.6 0 17 272 33 777.6 0 33 273 158 777.6 0 158 274 72 777.6 0 72 275 89 777.6 0 89 276 43 777.6 0 43 277 42 777.6 0 42 278 37 777.6 0 37 279 44 777.6 0 44 280 128 777.6 0 128 281 66 777.6 0 66 282 72 777.6 0 72 283 50 777.6 0 50 284 70 777.6 0 70 285 66 777.6 0 66 286 49 777.6 0 49 287 148 777.6 0 148 288 112 777.6 0 112 289 101 777.6 0 101 290 20 777.6 0 20 291 15 777.6 0 15 292 47 777.6 0 47 293 64 777.6 0 64 294 73 777.6 0 73 295 29 777.6 0 29 296 25 777.6 0 25 297 17 777.6 0 17 298 56 777.6 0 56 299 59 777.6 0 59 300 64 777.6 0 64 301 65 777.6 0 65 Finished in 0.66 s (47 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 13,915 Reads with adapters: 2,458 (17.7%) Reads that were too short: 153 (1.1%) Reads written (passing filters): 2,305 (16.6%) Total basepairs processed: 3,072,832 bp Total written (filtered): 636,103 bp (20.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2458 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 17 0.0 3 0 17 17 1770 0.0 3 1734 34 2 18 11 0.0 3 0 10 1 19 491 0.0 3 481 7 0 3 20 8 0.0 3 3 2 0 3 23 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 160 147 0.0 3 0 0 1 146 204 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 2,305 Reads with adapters: 149 (6.5%) Reads written (passing filters): 2,305 (100.0%) Total basepairs processed: 636,103 bp Total written (filtered): 635,656 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 149 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.1% C: 61.7% G: 17.4% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 149 36.0 0 149