This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 105.33 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 120,156 (3.8%) Reads that were too short: 38,165 (1.2%) Reads written (passing filters): 81,991 (2.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 21,696,762 bp (2.3%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 120156 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1423 777.6 0 1423 7 4328 777.6 0 4328 8 720 777.6 0 720 9 56446 777.6 0 56446 10 63 777.6 0 63 11 1323 777.6 0 1323 12 35 777.6 0 35 13 3 777.6 0 3 14 2 777.6 0 2 15 3 777.6 0 3 16 4 777.6 0 4 17 2 777.6 0 2 18 4 777.6 0 4 19 5 777.6 0 5 20 2 777.6 0 2 21 4 777.6 0 4 22 4 777.6 0 4 23 5 777.6 0 5 24 11 777.6 0 11 25 1 777.6 0 1 26 3 777.6 0 3 27 11 777.6 0 11 28 11 777.6 0 11 29 29 777.6 0 29 30 8 777.6 0 8 31 4 777.6 0 4 32 4 777.6 0 4 33 6 777.6 0 6 34 6 777.6 0 6 35 1 777.6 0 1 36 7 777.6 0 7 37 14 777.6 0 14 38 9 777.6 0 9 39 9 777.6 0 9 40 5 777.6 0 5 41 5 777.6 0 5 42 2 777.6 0 2 43 10 777.6 0 10 44 7 777.6 0 7 45 9 777.6 0 9 46 6 777.6 0 6 47 8 777.6 0 8 48 9 777.6 0 9 49 6 777.6 0 6 50 4 777.6 0 4 51 4 777.6 0 4 52 15 777.6 0 15 53 32 777.6 0 32 54 8 777.6 0 8 55 14 777.6 0 14 56 11 777.6 0 11 57 7 777.6 0 7 58 6 777.6 0 6 59 15 777.6 0 15 60 12 777.6 0 12 61 53 777.6 0 53 62 182 777.6 0 182 63 99 777.6 0 99 64 140 777.6 0 140 65 14 777.6 0 14 66 10 777.6 0 10 67 21 777.6 0 21 68 6 777.6 0 6 69 21 777.6 0 21 70 11 777.6 0 11 71 12 777.6 0 12 72 18 777.6 0 18 73 21 777.6 0 21 74 44 777.6 0 44 75 28 777.6 0 28 76 54 777.6 0 54 77 81 777.6 0 81 78 798 777.6 0 798 79 539 777.6 0 539 80 674 777.6 0 674 81 704 777.6 0 704 82 190 777.6 0 190 83 182 777.6 0 182 84 74 777.6 0 74 85 90 777.6 0 90 86 25 777.6 0 25 87 96 777.6 0 96 88 48 777.6 0 48 89 42 777.6 0 42 90 44 777.6 0 44 91 34 777.6 0 34 92 109 777.6 0 109 93 40 777.6 0 40 94 71 777.6 0 71 95 80 777.6 0 80 96 37 777.6 0 37 97 24 777.6 0 24 98 49 777.6 0 49 99 18 777.6 0 18 100 35 777.6 0 35 101 17 777.6 0 17 102 20 777.6 0 20 103 13 777.6 0 13 104 38 777.6 0 38 105 12 777.6 0 12 106 10 777.6 0 10 107 15 777.6 0 15 108 26 777.6 0 26 109 81 777.6 0 81 110 55 777.6 0 55 111 38 777.6 0 38 112 16 777.6 0 16 113 40 777.6 0 40 114 32 777.6 0 32 115 49 777.6 0 49 116 559 777.6 0 559 117 210 777.6 0 210 118 203 777.6 0 203 119 62 777.6 0 62 120 55 777.6 0 55 121 89 777.6 0 89 122 95 777.6 0 95 123 95 777.6 0 95 124 119 777.6 0 119 125 89 777.6 0 89 126 91 777.6 0 91 127 62 777.6 0 62 128 88 777.6 0 88 129 64 777.6 0 64 130 104 777.6 0 104 131 93 777.6 0 93 132 43 777.6 0 43 133 64 777.6 0 64 134 59 777.6 0 59 135 100 777.6 0 100 136 80 777.6 0 80 137 93 777.6 0 93 138 91 777.6 0 91 139 104 777.6 0 104 140 63 777.6 0 63 141 133 777.6 0 133 142 137 777.6 0 137 143 73 777.6 0 73 144 98 777.6 0 98 145 85 777.6 0 85 146 80 777.6 0 80 147 80 777.6 0 80 148 52 777.6 0 52 149 61 777.6 0 61 150 56 777.6 0 56 151 79 777.6 0 79 152 101 777.6 0 101 153 80 777.6 0 80 154 65 777.6 0 65 155 123 777.6 0 123 156 122 777.6 0 122 157 138 777.6 0 138 158 134 777.6 0 134 159 107 777.6 0 107 160 128 777.6 0 128 161 98 777.6 0 98 162 172 777.6 0 172 163 119 777.6 0 119 164 127 777.6 0 127 165 111 777.6 0 111 166 126 777.6 0 126 167 180 777.6 0 180 168 201 777.6 0 201 169 102 777.6 0 102 170 118 777.6 0 118 171 177 777.6 0 177 172 122 777.6 0 122 173 89 777.6 0 89 174 54 777.6 0 54 175 85 777.6 0 85 176 94 777.6 0 94 177 183 777.6 0 183 178 170 777.6 0 170 179 205 777.6 0 205 180 154 777.6 0 154 181 1031 777.6 0 1031 182 195 777.6 0 195 183 455 777.6 0 455 184 131 777.6 0 131 185 154 777.6 0 154 186 160 777.6 0 160 187 264 777.6 0 264 188 144 777.6 0 144 189 166 777.6 0 166 190 423 777.6 0 423 191 218 777.6 0 218 192 166 777.6 0 166 193 108 777.6 0 108 194 61 777.6 0 61 195 121 777.6 0 121 196 172 777.6 0 172 197 243 777.6 0 243 198 123 777.6 0 123 199 87 777.6 0 87 200 75 777.6 0 75 201 115 777.6 0 115 202 129 777.6 0 129 203 135 777.6 0 135 204 129 777.6 0 129 205 136 777.6 0 136 206 101 777.6 0 101 207 198 777.6 0 198 208 91 777.6 0 91 209 190 777.6 0 190 210 212 777.6 0 212 211 114 777.6 0 114 212 377 777.6 0 377 213 189 777.6 0 189 214 189 777.6 0 189 215 157 777.6 0 157 216 245 777.6 0 245 217 547 777.6 0 547 218 367 777.6 0 367 219 240 777.6 0 240 220 179 777.6 0 179 221 419 777.6 0 419 222 237 777.6 0 237 223 193 777.6 0 193 224 125 777.6 0 125 225 1021 777.6 0 1021 226 3679 777.6 0 3679 227 2889 777.6 0 2889 228 244 777.6 0 244 229 215 777.6 0 215 230 269 777.6 0 269 231 125 777.6 0 125 232 116 777.6 0 116 233 149 777.6 0 149 234 105 777.6 0 105 235 167 777.6 0 167 236 131 777.6 0 131 237 662 777.6 0 662 238 924 777.6 0 924 239 268 777.6 0 268 240 154 777.6 0 154 241 168 777.6 0 168 242 134 777.6 0 134 243 129 777.6 0 129 244 138 777.6 0 138 245 159 777.6 0 159 246 191 777.6 0 191 247 467 777.6 0 467 248 180 777.6 0 180 249 293 777.6 0 293 250 243 777.6 0 243 251 209 777.6 0 209 252 140 777.6 0 140 253 151 777.6 0 151 254 96 777.6 0 96 255 109 777.6 0 109 256 130 777.6 0 130 257 130 777.6 0 130 258 139 777.6 0 139 259 154 777.6 0 154 260 737 777.6 0 737 261 794 777.6 0 794 262 1315 777.6 0 1315 263 605 777.6 0 605 264 229 777.6 0 229 265 448 777.6 0 448 266 161 777.6 0 161 267 144 777.6 0 144 268 180 777.6 0 180 269 171 777.6 0 171 270 279 777.6 0 279 271 303 777.6 0 303 272 306 777.6 0 306 273 226 777.6 0 226 274 127 777.6 0 127 275 120 777.6 0 120 276 297 777.6 0 297 277 310 777.6 0 310 278 324 777.6 0 324 279 330 777.6 0 330 280 211 777.6 0 211 281 252 777.6 0 252 282 309 777.6 0 309 283 403 777.6 0 403 284 279 777.6 0 279 285 1137 777.6 0 1137 286 942 777.6 0 942 287 4092 777.6 0 4092 288 545 777.6 0 545 289 422 777.6 0 422 290 430 777.6 0 430 291 357 777.6 0 357 292 129 777.6 0 129 293 126 777.6 0 126 294 221 777.6 0 221 295 302 777.6 0 302 296 611 777.6 0 611 297 182 777.6 0 182 298 215 777.6 0 215 299 89 777.6 0 89 300 73 777.6 0 73 301 55 777.6 0 55 Finished in 5.01 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 81,991 Reads with adapters: 5,414 (6.6%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 5,391 (6.6%) Total basepairs processed: 21,696,762 bp Total written (filtered): 1,394,646 bp (6.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5414 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 17 0.0 3 0 14 2 0 1 20 3715 0.0 4 3631 76 4 4 21 6 0.0 4 3 2 0 0 1 22 16 0.0 4 1 12 2 1 23 896 0.0 4 872 15 5 3 1 24 1 0.0 4 1 81 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 2 0.0 4 0 0 0 0 2 127 728 0.0 4 0 0 0 1 727 128 2 0.0 4 0 0 0 0 2 149 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 183 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 1 4 230 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 2 0.0 4 0 0 0 0 2 285 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 5,391 Reads with adapters: 172 (3.2%) Reads written (passing filters): 5,391 (100.0%) Total basepairs processed: 1,394,646 bp Total written (filtered): 1,387,940 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 172 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.2% C: 6.4% G: 84.9% T: 3.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 84.2 0 3 5 1 5.3 0 1 6 1 1.3 0 1 9 5 0.0 0 1 4 10 18 0.0 1 0 18 11 115 0.0 1 0 115 12 9 0.0 1 0 9 210 2 0.0 1 2 223 1 0.0 1 1 238 3 0.0 1 3 264 3 0.0 1 3 267 10 0.0 1 10 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.