This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 100.69 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 112,972 (3.5%) Reads that were too short: 10,307 (0.3%) Reads written (passing filters): 102,665 (3.2%) Total basepairs processed: 958,672,960 bp Total written (filtered): 25,432,269 bp (2.7%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 112972 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 419 777.6 0 419 7 719 777.6 0 719 8 45743 777.6 0 45743 9 10093 777.6 0 10093 10 31 777.6 0 31 11 19 777.6 0 19 12 13 777.6 0 13 13 23 777.6 0 23 14 4 777.6 0 4 15 9 777.6 0 9 16 8 777.6 0 8 17 83 777.6 0 83 18 13 777.6 0 13 19 6 777.6 0 6 20 5 777.6 0 5 21 10 777.6 0 10 22 12 777.6 0 12 23 6 777.6 0 6 24 8 777.6 0 8 25 49 777.6 0 49 26 27 777.6 0 27 27 8 777.6 0 8 28 12 777.6 0 12 29 8 777.6 0 8 30 14 777.6 0 14 31 175 777.6 0 175 32 132 777.6 0 132 33 18 777.6 0 18 34 14 777.6 0 14 35 11 777.6 0 11 36 4 777.6 0 4 37 5 777.6 0 5 38 20 777.6 0 20 39 35 777.6 0 35 40 23 777.6 0 23 41 20 777.6 0 20 42 14 777.6 0 14 43 11 777.6 0 11 44 18 777.6 0 18 45 13 777.6 0 13 46 6 777.6 0 6 47 33 777.6 0 33 48 148 777.6 0 148 49 56 777.6 0 56 50 25 777.6 0 25 51 42 777.6 0 42 52 13 777.6 0 13 53 15 777.6 0 15 54 8 777.6 0 8 55 83 777.6 0 83 56 304 777.6 0 304 57 49 777.6 0 49 58 9 777.6 0 9 59 7 777.6 0 7 60 25 777.6 0 25 61 69 777.6 0 69 62 18 777.6 0 18 63 9 777.6 0 9 64 14 777.6 0 14 65 30 777.6 0 30 66 268 777.6 0 268 67 60 777.6 0 60 68 9 777.6 0 9 69 16 777.6 0 16 70 20 777.6 0 20 71 63 777.6 0 63 72 98 777.6 0 98 73 13 777.6 0 13 74 9 777.6 0 9 75 23 777.6 0 23 76 65 777.6 0 65 77 57 777.6 0 57 78 86 777.6 0 86 79 77 777.6 0 77 80 67 777.6 0 67 81 91 777.6 0 91 82 156 777.6 0 156 83 96 777.6 0 96 84 167 777.6 0 167 85 200 777.6 0 200 86 1275 777.6 0 1275 87 484 777.6 0 484 88 528 777.6 0 528 89 216 777.6 0 216 90 503 777.6 0 503 91 50 777.6 0 50 92 97 777.6 0 97 93 141 777.6 0 141 94 167 777.6 0 167 95 57 777.6 0 57 96 60 777.6 0 60 97 69 777.6 0 69 98 117 777.6 0 117 99 129 777.6 0 129 100 184 777.6 0 184 101 413 777.6 0 413 102 563 777.6 0 563 103 8871 777.6 0 8871 104 12616 777.6 0 12616 105 5030 777.6 0 5030 106 313 777.6 0 313 107 175 777.6 0 175 108 221 777.6 0 221 109 29 777.6 0 29 110 33 777.6 0 33 111 71 777.6 0 71 112 110 777.6 0 110 113 30 777.6 0 30 114 34 777.6 0 34 115 33 777.6 0 33 116 28 777.6 0 28 117 16 777.6 0 16 118 42 777.6 0 42 119 204 777.6 0 204 120 121 777.6 0 121 121 153 777.6 0 153 122 59 777.6 0 59 123 30 777.6 0 30 124 64 777.6 0 64 125 267 777.6 0 267 126 210 777.6 0 210 127 43 777.6 0 43 128 71 777.6 0 71 129 55 777.6 0 55 130 48 777.6 0 48 131 31 777.6 0 31 132 97 777.6 0 97 133 280 777.6 0 280 134 328 777.6 0 328 135 92 777.6 0 92 136 92 777.6 0 92 137 103 777.6 0 103 138 208 777.6 0 208 139 691 777.6 0 691 140 246 777.6 0 246 141 190 777.6 0 190 142 63 777.6 0 63 143 138 777.6 0 138 144 155 777.6 0 155 145 237 777.6 0 237 146 66 777.6 0 66 147 51 777.6 0 51 148 49 777.6 0 49 149 150 777.6 0 150 150 378 777.6 0 378 151 91 777.6 0 91 152 108 777.6 0 108 153 136 777.6 0 136 154 111 777.6 0 111 155 105 777.6 0 105 156 78 777.6 0 78 157 86 777.6 0 86 158 119 777.6 0 119 159 265 777.6 0 265 160 177 777.6 0 177 161 82 777.6 0 82 162 54 777.6 0 54 163 57 777.6 0 57 164 117 777.6 0 117 165 134 777.6 0 134 166 98 777.6 0 98 167 51 777.6 0 51 168 60 777.6 0 60 169 50 777.6 0 50 170 42 777.6 0 42 171 110 777.6 0 110 172 234 777.6 0 234 173 159 777.6 0 159 174 32 777.6 0 32 175 20 777.6 0 20 176 14 777.6 0 14 177 18 777.6 0 18 178 83 777.6 0 83 179 87 777.6 0 87 180 152 777.6 0 152 181 106 777.6 0 106 182 70 777.6 0 70 183 66 777.6 0 66 184 159 777.6 0 159 185 222 777.6 0 222 186 68 777.6 0 68 187 43 777.6 0 43 188 43 777.6 0 43 189 168 777.6 0 168 190 144 777.6 0 144 191 132 777.6 0 132 192 32 777.6 0 32 193 25 777.6 0 25 194 33 777.6 0 33 195 26 777.6 0 26 196 18 777.6 0 18 197 50 777.6 0 50 198 29 777.6 0 29 199 22 777.6 0 22 200 75 777.6 0 75 201 70 777.6 0 70 202 53 777.6 0 53 203 43 777.6 0 43 204 74 777.6 0 74 205 225 777.6 0 225 206 317 777.6 0 317 207 313 777.6 0 313 208 175 777.6 0 175 209 134 777.6 0 134 210 150 777.6 0 150 211 138 777.6 0 138 212 113 777.6 0 113 213 117 777.6 0 117 214 115 777.6 0 115 215 122 777.6 0 122 216 105 777.6 0 105 217 71 777.6 0 71 218 117 777.6 0 117 219 176 777.6 0 176 220 212 777.6 0 212 221 80 777.6 0 80 222 48 777.6 0 48 223 136 777.6 0 136 224 359 777.6 0 359 225 298 777.6 0 298 226 146 777.6 0 146 227 176 777.6 0 176 228 72 777.6 0 72 229 59 777.6 0 59 230 46 777.6 0 46 231 65 777.6 0 65 232 19 777.6 0 19 233 34 777.6 0 34 234 42 777.6 0 42 235 153 777.6 0 153 236 43 777.6 0 43 237 27 777.6 0 27 238 14 777.6 0 14 239 10 777.6 0 10 240 22 777.6 0 22 241 27 777.6 0 27 242 29 777.6 0 29 243 26 777.6 0 26 244 55 777.6 0 55 245 64 777.6 0 64 246 104 777.6 0 104 247 25 777.6 0 25 248 29 777.6 0 29 249 20 777.6 0 20 250 28 777.6 0 28 251 43 777.6 0 43 252 37 777.6 0 37 253 84 777.6 0 84 254 383 777.6 0 383 255 623 777.6 0 623 256 157 777.6 0 157 257 61 777.6 0 61 258 57 777.6 0 57 259 196 777.6 0 196 260 147 777.6 0 147 261 302 777.6 0 302 262 310 777.6 0 310 263 383 777.6 0 383 264 81 777.6 0 81 265 221 777.6 0 221 266 112 777.6 0 112 267 56 777.6 0 56 268 31 777.6 0 31 269 41 777.6 0 41 270 12 777.6 0 12 271 12 777.6 0 12 272 19 777.6 0 19 273 20 777.6 0 20 274 60 777.6 0 60 275 30 777.6 0 30 276 51 777.6 0 51 277 21 777.6 0 21 278 60 777.6 0 60 279 164 777.6 0 164 280 89 777.6 0 89 281 178 777.6 0 178 282 49 777.6 0 49 283 39 777.6 0 39 284 47 777.6 0 47 285 56 777.6 0 56 286 147 777.6 0 147 287 31 777.6 0 31 288 19 777.6 0 19 289 21 777.6 0 21 290 25 777.6 0 25 291 38 777.6 0 38 292 31 777.6 0 31 293 32 777.6 0 32 294 24 777.6 0 24 295 35 777.6 0 35 296 37 777.6 0 37 297 115 777.6 0 115 298 124 777.6 0 124 299 64 777.6 0 64 300 43 777.6 0 43 301 263 777.6 0 263 Finished in 5.85 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 102,665 Reads with adapters: 10,444 (10.2%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 10,426 (10.2%) Total basepairs processed: 25,432,269 bp Total written (filtered): 2,823,357 bp (11.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10444 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 30 0.0 3 0 22 3 2 3 20 10226 0.0 4 9895 275 42 9 5 21 29 0.0 4 2 23 3 0 1 22 3 0.0 4 1 0 2 23 11 0.0 4 0 0 0 8 3 32 1 0.0 4 0 1 45 2 0.0 4 2 78 1 0.0 4 1 94 2 0.0 4 0 0 0 0 2 127 115 0.0 4 0 0 0 0 115 129 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 191 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 284 2 0.0 4 0 0 0 0 2 289 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.46 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 10,426 Reads with adapters: 687 (6.6%) Reads written (passing filters): 10,426 (100.0%) Total basepairs processed: 2,823,357 bp Total written (filtered): 2,814,568 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 687 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.6% C: 3.1% G: 90.5% T: 2.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 5 1 10.2 0 1 6 1 2.5 0 1 9 109 0.0 0 5 104 10 188 0.0 1 9 179 11 374 0.0 1 0 374 12 2 0.0 1 0 2 13 1 0.0 1 0 1 150 9 0.0 1 7 2 208 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.