This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.45 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 86,723 (2.7%) Reads that were too short: 22,493 (0.7%) Reads written (passing filters): 64,230 (2.0%) Total basepairs processed: 958,672,960 bp Total written (filtered): 11,804,378 bp (1.2%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 86723 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5738 777.6 0 5738 7 567 777.6 0 567 8 15198 777.6 0 15198 9 40 777.6 0 40 10 5 777.6 0 5 11 3 777.6 0 3 12 2 777.6 0 2 13 8 777.6 0 8 14 6 777.6 0 6 15 5 777.6 0 5 16 5 777.6 0 5 17 5 777.6 0 5 18 4 777.6 0 4 19 4 777.6 0 4 20 7 777.6 0 7 21 3 777.6 0 3 22 5 777.6 0 5 23 9 777.6 0 9 24 6 777.6 0 6 25 1 777.6 0 1 26 5 777.6 0 5 27 8 777.6 0 8 28 7 777.6 0 7 29 10 777.6 0 10 30 13 777.6 0 13 31 7 777.6 0 7 32 16 777.6 0 16 33 14 777.6 0 14 34 15 777.6 0 15 35 8 777.6 0 8 36 6 777.6 0 6 37 8 777.6 0 8 38 7 777.6 0 7 39 10 777.6 0 10 40 267 777.6 0 267 41 3 777.6 0 3 42 7 777.6 0 7 43 7 777.6 0 7 44 6 777.6 0 6 45 6 777.6 0 6 46 8 777.6 0 8 47 9 777.6 0 9 48 7 777.6 0 7 49 4 777.6 0 4 50 13 777.6 0 13 51 14 777.6 0 14 52 3 777.6 0 3 53 14 777.6 0 14 54 27 777.6 0 27 55 8 777.6 0 8 56 8 777.6 0 8 57 10 777.6 0 10 58 3 777.6 0 3 59 11 777.6 0 11 60 97 777.6 0 97 61 8 777.6 0 8 62 6 777.6 0 6 63 6 777.6 0 6 64 14 777.6 0 14 65 20 777.6 0 20 66 26 777.6 0 26 67 8 777.6 0 8 68 18 777.6 0 18 69 6 777.6 0 6 70 91 777.6 0 91 71 16 777.6 0 16 72 5 777.6 0 5 73 3 777.6 0 3 74 4 777.6 0 4 75 12 777.6 0 12 76 19 777.6 0 19 77 11 777.6 0 11 78 5 777.6 0 5 79 66 777.6 0 66 80 50 777.6 0 50 81 82 777.6 0 82 82 12 777.6 0 12 83 7 777.6 0 7 84 8 777.6 0 8 85 9 777.6 0 9 86 5 777.6 0 5 87 7 777.6 0 7 88 3 777.6 0 3 89 23 777.6 0 23 90 10 777.6 0 10 91 7 777.6 0 7 92 11 777.6 0 11 93 8 777.6 0 8 94 5 777.6 0 5 95 12 777.6 0 12 96 43 777.6 0 43 97 136 777.6 0 136 98 14 777.6 0 14 99 14 777.6 0 14 100 22 777.6 0 22 101 16 777.6 0 16 102 21 777.6 0 21 103 31 777.6 0 31 104 37 777.6 0 37 105 3 777.6 0 3 106 4 777.6 0 4 107 32 777.6 0 32 108 16 777.6 0 16 109 74 777.6 0 74 110 21 777.6 0 21 111 13 777.6 0 13 112 15 777.6 0 15 113 26 777.6 0 26 114 17 777.6 0 17 115 19 777.6 0 19 116 44 777.6 0 44 117 62 777.6 0 62 118 93 777.6 0 93 119 66 777.6 0 66 120 22 777.6 0 22 121 16 777.6 0 16 122 17 777.6 0 17 123 16 777.6 0 16 124 16 777.6 0 16 125 15 777.6 0 15 126 16 777.6 0 16 127 35 777.6 0 35 128 43 777.6 0 43 129 34 777.6 0 34 130 14 777.6 0 14 131 55 777.6 0 55 132 36 777.6 0 36 133 81 777.6 0 81 134 29 777.6 0 29 135 28 777.6 0 28 136 31 777.6 0 31 137 31 777.6 0 31 138 48 777.6 0 48 139 18 777.6 0 18 140 39 777.6 0 39 141 137 777.6 0 137 142 48 777.6 0 48 143 20 777.6 0 20 144 10 777.6 0 10 145 19 777.6 0 19 146 30 777.6 0 30 147 55 777.6 0 55 148 36 777.6 0 36 149 13 777.6 0 13 150 11 777.6 0 11 151 15 777.6 0 15 152 24 777.6 0 24 153 22 777.6 0 22 154 39 777.6 0 39 155 25 777.6 0 25 156 75 777.6 0 75 157 50 777.6 0 50 158 50 777.6 0 50 159 36 777.6 0 36 160 57 777.6 0 57 161 109 777.6 0 109 162 101 777.6 0 101 163 106 777.6 0 106 164 197 777.6 0 197 165 268 777.6 0 268 166 140 777.6 0 140 167 189 777.6 0 189 168 207 777.6 0 207 169 154 777.6 0 154 170 134 777.6 0 134 171 197 777.6 0 197 172 154 777.6 0 154 173 192 777.6 0 192 174 171 777.6 0 171 175 255 777.6 0 255 176 826 777.6 0 826 177 6224 777.6 0 6224 178 14718 777.6 0 14718 179 2276 777.6 0 2276 180 6812 777.6 0 6812 181 1451 777.6 0 1451 182 1371 777.6 0 1371 183 174 777.6 0 174 184 37 777.6 0 37 185 28 777.6 0 28 186 28 777.6 0 28 187 68 777.6 0 68 188 66 777.6 0 66 189 33 777.6 0 33 190 16 777.6 0 16 191 46 777.6 0 46 192 20 777.6 0 20 193 27 777.6 0 27 194 124 777.6 0 124 195 310 777.6 0 310 196 425 777.6 0 425 197 355 777.6 0 355 198 519 777.6 0 519 199 67 777.6 0 67 200 357 777.6 0 357 201 137 777.6 0 137 202 109 777.6 0 109 203 53 777.6 0 53 204 16 777.6 0 16 205 13 777.6 0 13 206 22 777.6 0 22 207 28 777.6 0 28 208 20 777.6 0 20 209 19 777.6 0 19 210 22 777.6 0 22 211 17 777.6 0 17 212 18 777.6 0 18 213 34 777.6 0 34 214 36 777.6 0 36 215 155 777.6 0 155 216 57 777.6 0 57 217 91 777.6 0 91 218 23 777.6 0 23 219 27 777.6 0 27 220 59 777.6 0 59 221 32 777.6 0 32 222 36 777.6 0 36 223 47 777.6 0 47 224 80 777.6 0 80 225 97 777.6 0 97 226 52 777.6 0 52 227 36 777.6 0 36 228 44 777.6 0 44 229 66 777.6 0 66 230 36 777.6 0 36 231 42 777.6 0 42 232 40 777.6 0 40 233 39 777.6 0 39 234 168 777.6 0 168 235 122 777.6 0 122 236 342 777.6 0 342 237 183 777.6 0 183 238 88 777.6 0 88 239 665 777.6 0 665 240 434 777.6 0 434 241 814 777.6 0 814 242 288 777.6 0 288 243 151 777.6 0 151 244 27 777.6 0 27 245 37 777.6 0 37 246 75 777.6 0 75 247 53 777.6 0 53 248 43 777.6 0 43 249 90 777.6 0 90 250 203 777.6 0 203 251 45 777.6 0 45 252 69 777.6 0 69 253 69 777.6 0 69 254 98 777.6 0 98 255 161 777.6 0 161 256 83 777.6 0 83 257 118 777.6 0 118 258 121 777.6 0 121 259 588 777.6 0 588 260 965 777.6 0 965 261 1907 777.6 0 1907 262 134 777.6 0 134 263 345 777.6 0 345 264 791 777.6 0 791 265 931 777.6 0 931 266 839 777.6 0 839 267 164 777.6 0 164 268 33 777.6 0 33 269 34 777.6 0 34 270 30 777.6 0 30 271 7 777.6 0 7 272 5 777.6 0 5 273 19 777.6 0 19 274 18 777.6 0 18 275 62 777.6 0 62 276 39 777.6 0 39 277 16 777.6 0 16 278 27 777.6 0 27 279 16 777.6 0 16 280 50 777.6 0 50 281 58 777.6 0 58 282 33 777.6 0 33 283 15 777.6 0 15 284 20 777.6 0 20 285 20 777.6 0 20 286 63 777.6 0 63 287 108 777.6 0 108 288 257 777.6 0 257 289 217 777.6 0 217 290 115 777.6 0 115 291 482 777.6 0 482 292 939 777.6 0 939 293 5983 777.6 0 5983 294 336 777.6 0 336 295 234 777.6 0 234 296 181 777.6 0 181 297 172 777.6 0 172 298 62 777.6 0 62 299 61 777.6 0 61 300 70 777.6 0 70 301 34 777.6 0 34 Finished in 2.63 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 64,230 Reads with adapters: 16,263 (25.3%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 16,254 (25.3%) Total basepairs processed: 11,804,378 bp Total written (filtered): 4,479,536 bp (37.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16263 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 3 0.0 3 0 0 3 16 88 0.0 3 0 86 0 2 17 10438 0.0 3 10253 178 7 18 46 0.0 3 2 31 12 1 19 98 0.0 3 9 61 24 4 20 5565 0.0 3 5424 108 8 25 21 6 0.0 3 0 6 23 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 216 4 0.0 3 0 0 0 4 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.74 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 16,254 Reads with adapters: 381 (2.3%) Reads written (passing filters): 16,254 (100.0%) Total basepairs processed: 4,479,536 bp Total written (filtered): 4,478,381 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 381 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.8% C: 16.8% G: 49.9% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 369 254.0 0 369 4 12 63.5 0 12