This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 106.73 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 864,721 (27.2%) Reads that were too short: 106,395 (3.3%) Reads written (passing filters): 758,326 (23.8%) Total basepairs processed: 958,672,960 bp Total written (filtered): 207,690,237 bp (21.7%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 864721 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 172 777.6 0 172 7 9933 777.6 0 9933 8 2143 777.6 0 2143 9 169 777.6 0 169 10 23 777.6 0 23 11 45 777.6 0 45 12 24 777.6 0 24 13 29 777.6 0 29 14 22 777.6 0 22 15 24 777.6 0 24 16 60 777.6 0 60 17 23 777.6 0 23 18 44 777.6 0 44 19 145 777.6 0 145 20 620373 777.6 0 620373 21 47735 777.6 0 47735 22 208 777.6 0 208 23 61 777.6 0 61 24 49 777.6 0 49 25 73 777.6 0 73 26 1032 777.6 0 1032 27 19 777.6 0 19 28 34 777.6 0 34 29 37 777.6 0 37 30 41 777.6 0 41 31 71 777.6 0 71 32 156 777.6 0 156 33 13026 777.6 0 13026 34 708 777.6 0 708 35 91 777.6 0 91 36 50 777.6 0 50 37 41 777.6 0 41 38 76 777.6 0 76 39 71 777.6 0 71 40 70 777.6 0 70 41 96 777.6 0 96 42 66 777.6 0 66 43 59 777.6 0 59 44 103 777.6 0 103 45 45 777.6 0 45 46 85 777.6 0 85 47 313 777.6 0 313 48 65 777.6 0 65 49 76 777.6 0 76 50 78 777.6 0 78 51 86 777.6 0 86 52 77 777.6 0 77 53 157 777.6 0 157 54 94 777.6 0 94 55 135 777.6 0 135 56 185 777.6 0 185 57 170 777.6 0 170 58 200 777.6 0 200 59 171 777.6 0 171 60 246 777.6 0 246 61 258 777.6 0 258 62 264 777.6 0 264 63 276 777.6 0 276 64 239 777.6 0 239 65 215 777.6 0 215 66 271 777.6 0 271 67 353 777.6 0 353 68 308 777.6 0 308 69 412 777.6 0 412 70 400 777.6 0 400 71 534 777.6 0 534 72 400 777.6 0 400 73 485 777.6 0 485 74 438 777.6 0 438 75 1922 777.6 0 1922 76 1251 777.6 0 1251 77 1049 777.6 0 1049 78 994 777.6 0 994 79 6160 777.6 0 6160 80 1558 777.6 0 1558 81 6151 777.6 0 6151 82 474 777.6 0 474 83 423 777.6 0 423 84 458 777.6 0 458 85 1612 777.6 0 1612 86 479 777.6 0 479 87 339 777.6 0 339 88 758 777.6 0 758 89 293 777.6 0 293 90 814 777.6 0 814 91 257 777.6 0 257 92 309 777.6 0 309 93 261 777.6 0 261 94 280 777.6 0 280 95 209 777.6 0 209 96 199 777.6 0 199 97 231 777.6 0 231 98 215 777.6 0 215 99 194 777.6 0 194 100 196 777.6 0 196 101 214 777.6 0 214 102 202 777.6 0 202 103 221 777.6 0 221 104 206 777.6 0 206 105 185 777.6 0 185 106 172 777.6 0 172 107 195 777.6 0 195 108 196 777.6 0 196 109 340 777.6 0 340 110 164 777.6 0 164 111 181 777.6 0 181 112 194 777.6 0 194 113 184 777.6 0 184 114 191 777.6 0 191 115 208 777.6 0 208 116 229 777.6 0 229 117 247 777.6 0 247 118 200 777.6 0 200 119 200 777.6 0 200 120 206 777.6 0 206 121 216 777.6 0 216 122 519 777.6 0 519 123 449 777.6 0 449 124 1075 777.6 0 1075 125 197 777.6 0 197 126 199 777.6 0 199 127 186 777.6 0 186 128 201 777.6 0 201 129 213 777.6 0 213 130 1280 777.6 0 1280 131 982 777.6 0 982 132 780 777.6 0 780 133 187 777.6 0 187 134 225 777.6 0 225 135 221 777.6 0 221 136 1825 777.6 0 1825 137 1291 777.6 0 1291 138 2588 777.6 0 2588 139 450 777.6 0 450 140 274 777.6 0 274 141 216 777.6 0 216 142 147 777.6 0 147 143 111 777.6 0 111 144 132 777.6 0 132 145 166 777.6 0 166 146 157 777.6 0 157 147 175 777.6 0 175 148 167 777.6 0 167 149 134 777.6 0 134 150 138 777.6 0 138 151 139 777.6 0 139 152 185 777.6 0 185 153 191 777.6 0 191 154 133 777.6 0 133 155 142 777.6 0 142 156 141 777.6 0 141 157 193 777.6 0 193 158 211 777.6 0 211 159 184 777.6 0 184 160 153 777.6 0 153 161 161 777.6 0 161 162 119 777.6 0 119 163 122 777.6 0 122 164 115 777.6 0 115 165 180 777.6 0 180 166 168 777.6 0 168 167 188 777.6 0 188 168 143 777.6 0 143 169 109 777.6 0 109 170 123 777.6 0 123 171 135 777.6 0 135 172 123 777.6 0 123 173 147 777.6 0 147 174 105 777.6 0 105 175 131 777.6 0 131 176 128 777.6 0 128 177 105 777.6 0 105 178 116 777.6 0 116 179 109 777.6 0 109 180 119 777.6 0 119 181 115 777.6 0 115 182 148 777.6 0 148 183 112 777.6 0 112 184 111 777.6 0 111 185 141 777.6 0 141 186 161 777.6 0 161 187 189 777.6 0 189 188 153 777.6 0 153 189 148 777.6 0 148 190 229 777.6 0 229 191 122 777.6 0 122 192 132 777.6 0 132 193 127 777.6 0 127 194 177 777.6 0 177 195 125 777.6 0 125 196 155 777.6 0 155 197 184 777.6 0 184 198 131 777.6 0 131 199 364 777.6 0 364 200 328 777.6 0 328 201 296 777.6 0 296 202 214 777.6 0 214 203 195 777.6 0 195 204 155 777.6 0 155 205 150 777.6 0 150 206 118 777.6 0 118 207 120 777.6 0 120 208 125 777.6 0 125 209 145 777.6 0 145 210 172 777.6 0 172 211 155 777.6 0 155 212 269 777.6 0 269 213 176 777.6 0 176 214 257 777.6 0 257 215 175 777.6 0 175 216 179 777.6 0 179 217 158 777.6 0 158 218 151 777.6 0 151 219 178 777.6 0 178 220 195 777.6 0 195 221 241 777.6 0 241 222 238 777.6 0 238 223 340 777.6 0 340 224 462 777.6 0 462 225 448 777.6 0 448 226 855 777.6 0 855 227 1231 777.6 0 1231 228 968 777.6 0 968 229 904 777.6 0 904 230 740 777.6 0 740 231 919 777.6 0 919 232 641 777.6 0 641 233 550 777.6 0 550 234 544 777.6 0 544 235 435 777.6 0 435 236 682 777.6 0 682 237 810 777.6 0 810 238 1884 777.6 0 1884 239 764 777.6 0 764 240 1439 777.6 0 1439 241 1355 777.6 0 1355 242 560 777.6 0 560 243 410 777.6 0 410 244 937 777.6 0 937 245 747 777.6 0 747 246 1643 777.6 0 1643 247 750 777.6 0 750 248 1384 777.6 0 1384 249 1079 777.6 0 1079 250 1058 777.6 0 1058 251 11929 777.6 0 11929 252 7821 777.6 0 7821 253 52137 777.6 0 52137 254 415 777.6 0 415 255 107 777.6 0 107 256 99 777.6 0 99 257 82 777.6 0 82 258 73 777.6 0 73 259 93 777.6 0 93 260 94 777.6 0 94 261 64 777.6 0 64 262 93 777.6 0 93 263 99 777.6 0 99 264 110 777.6 0 110 265 90 777.6 0 90 266 100 777.6 0 100 267 103 777.6 0 103 268 201 777.6 0 201 269 92 777.6 0 92 270 85 777.6 0 85 271 76 777.6 0 76 272 83 777.6 0 83 273 76 777.6 0 76 274 84 777.6 0 84 275 59 777.6 0 59 276 83 777.6 0 83 277 62 777.6 0 62 278 86 777.6 0 86 279 84 777.6 0 84 280 119 777.6 0 119 281 77 777.6 0 77 282 69 777.6 0 69 283 88 777.6 0 88 284 68 777.6 0 68 285 105 777.6 0 105 286 63 777.6 0 63 287 95 777.6 0 95 288 92 777.6 0 92 289 114 777.6 0 114 290 82 777.6 0 82 291 87 777.6 0 87 292 74 777.6 0 74 293 101 777.6 0 101 294 73 777.6 0 73 295 119 777.6 0 119 296 55 777.6 0 55 297 167 777.6 0 167 298 259 777.6 0 259 299 1485 777.6 0 1485 300 73 777.6 0 73 301 45 777.6 0 45 Finished in 43.52 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 758,326 Reads with adapters: 2,711 (0.4%) Reads that were too short: 295 (0.0%) Reads written (passing filters): 2,416 (0.3%) Total basepairs processed: 207,690,237 bp Total written (filtered): 627,548 bp (0.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2711 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 5 0.0 3 0 4 1 20 2117 0.0 4 2018 85 12 1 1 21 13 0.0 4 0 13 54 3 0.0 4 0 0 0 0 3 64 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 1 1 71 3 0.0 4 0 0 0 0 3 72 2 0.0 4 0 0 0 0 2 73 2 0.0 4 0 0 0 0 2 74 2 0.0 4 0 0 0 0 2 75 2 0.0 4 0 0 0 0 2 76 1 0.0 4 0 0 0 0 1 77 4 0.0 4 0 0 0 0 4 78 2 0.0 4 0 0 0 0 2 79 11 0.0 4 0 0 0 0 11 80 18 0.0 4 0 0 0 2 16 81 15 0.0 4 0 0 0 0 15 82 10 0.0 4 0 0 0 0 10 83 2 0.0 4 0 0 0 0 2 84 2 0.0 4 0 0 0 0 2 87 5 0.0 4 0 0 0 0 5 89 1 0.0 4 0 0 0 0 1 95 4 0.0 4 0 0 0 0 4 96 1 0.0 4 0 0 0 0 1 99 12 0.0 4 0 0 0 0 12 100 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 3 0.0 4 0 0 0 0 3 109 1 0.0 4 0 0 0 0 1 112 3 0.0 4 0 0 0 0 3 113 3 0.0 4 0 0 0 0 3 114 4 0.0 4 0 0 0 0 4 115 7 0.0 4 0 0 0 0 7 116 7 0.0 4 0 0 0 0 7 117 1 0.0 4 0 0 0 0 1 118 3 0.0 4 0 1 0 0 2 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 1 121 5 0.0 4 0 0 0 0 5 122 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 124 3 0.0 4 0 0 0 0 3 126 3 0.0 4 0 0 0 0 3 127 3 0.0 4 0 0 0 0 3 129 4 0.0 4 0 0 0 0 4 130 4 0.0 4 0 0 0 0 4 131 1 0.0 4 0 0 0 0 1 132 2 0.0 4 0 0 0 0 2 134 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 138 6 0.0 4 0 0 0 1 5 139 2 0.0 4 0 0 0 0 2 140 6 0.0 4 0 0 0 0 6 141 18 0.0 4 0 0 0 0 18 142 21 0.0 4 0 0 0 1 20 143 1 0.0 4 0 0 0 1 144 2 0.0 4 0 0 0 0 2 145 2 0.0 4 0 0 0 0 2 146 3 0.0 4 0 0 0 1 2 147 2 0.0 4 0 0 0 0 2 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 4 0.0 4 0 0 0 0 4 151 3 0.0 4 0 0 0 1 2 152 3 0.0 4 0 0 0 0 3 153 3 0.0 4 1 0 0 0 2 154 5 0.0 4 0 0 0 1 4 155 3 0.0 4 0 0 0 0 3 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 158 3 0.0 4 0 0 0 1 2 159 1 0.0 4 0 0 0 0 1 160 3 0.0 4 0 0 0 0 3 161 3 0.0 4 0 0 0 0 3 162 2 0.0 4 0 0 0 0 2 163 2 0.0 4 0 0 0 0 2 164 2 0.0 4 0 0 0 0 2 166 2 0.0 4 0 0 0 0 2 168 1 0.0 4 0 0 0 0 1 169 2 0.0 4 0 0 0 0 2 170 1 0.0 4 0 0 0 0 1 171 2 0.0 4 0 0 0 0 2 172 3 0.0 4 0 0 0 0 3 174 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 4 0.0 4 0 0 0 0 4 177 2 0.0 4 0 0 0 0 2 178 2 0.0 4 0 0 0 0 2 179 2 0.0 4 0 0 0 0 2 180 2 0.0 4 0 0 0 0 2 181 1 0.0 4 0 0 0 0 1 182 2 0.0 4 0 0 0 0 2 183 2 0.0 4 0 0 0 0 2 185 1 0.0 4 0 0 0 0 1 188 4 0.0 4 0 0 0 0 4 189 1 0.0 4 0 0 0 0 1 190 3 0.0 4 0 0 0 0 3 191 1 0.0 4 0 0 0 0 1 192 3 0.0 4 0 0 0 0 3 193 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 195 4 0.0 4 0 0 0 0 4 196 6 0.0 4 0 0 0 0 6 197 4 0.0 4 0 0 0 0 4 198 3 0.0 4 0 0 0 0 3 199 3 0.0 4 0 0 0 0 3 200 3 0.0 4 0 0 0 0 3 201 2 0.0 4 0 0 0 0 2 202 1 0.0 4 0 0 0 1 203 5 0.0 4 0 0 0 0 5 204 2 0.0 4 0 0 0 0 2 205 3 0.0 4 0 0 0 0 3 206 5 0.0 4 0 0 0 0 5 207 8 0.0 4 0 0 0 0 8 208 5 0.0 4 0 0 0 0 5 209 6 0.0 4 0 0 0 0 6 210 5 0.0 4 0 0 0 2 3 211 5 0.0 4 0 0 0 0 5 212 5 0.0 4 0 0 0 1 4 213 7 0.0 4 0 0 0 0 7 214 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 216 4 0.0 4 0 0 0 0 4 217 3 0.0 4 0 0 0 0 3 218 4 0.0 4 0 0 0 0 4 219 1 0.0 4 0 0 0 0 1 220 4 0.0 4 0 0 0 0 4 221 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 223 3 0.0 4 0 0 0 0 3 224 3 0.0 4 0 0 0 0 3 225 1 0.0 4 0 0 0 0 1 226 6 0.0 4 0 0 0 0 6 227 7 0.0 4 0 0 0 0 7 228 3 0.0 4 0 0 0 0 3 229 2 0.0 4 0 0 0 0 2 230 3 0.0 4 0 0 0 0 3 231 2 0.0 4 0 0 0 0 2 232 3 0.0 4 0 0 0 0 3 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 3 0.0 4 0 0 0 0 3 237 4 0.0 4 0 0 0 0 4 238 5 0.0 4 0 0 0 0 5 239 3 0.0 4 0 0 0 0 3 240 2 0.0 4 0 0 0 0 2 241 2 0.0 4 0 0 0 0 2 242 2 0.0 4 0 0 0 0 2 243 1 0.0 4 0 0 0 0 1 244 4 0.0 4 0 0 0 0 4 245 4 0.0 4 0 0 0 0 4 247 2 0.0 4 0 0 0 0 2 248 1 0.0 4 0 0 0 0 1 249 5 0.0 4 0 0 0 0 5 251 5 0.0 4 0 0 0 0 5 252 2 0.0 4 0 0 0 0 2 253 4 0.0 4 0 0 0 0 4 254 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 256 2 0.0 4 0 0 0 0 2 257 3 0.0 4 0 0 0 0 3 258 3 0.0 4 0 0 0 1 2 259 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 262 5 0.0 4 0 0 0 1 4 264 3 0.0 4 0 0 0 0 3 265 2 0.0 4 0 0 0 0 2 266 2 0.0 4 0 0 0 0 2 267 2 0.0 4 0 0 0 0 2 268 1 0.0 4 0 0 0 0 1 269 2 0.0 4 0 0 0 0 2 270 2 0.0 4 0 0 0 0 2 271 4 0.0 4 0 0 0 0 4 272 4 0.0 4 0 0 0 0 4 273 3 0.0 4 0 0 0 0 3 274 3 0.0 4 0 0 0 0 3 275 3 0.0 4 0 0 0 0 3 276 1 0.0 4 0 0 0 0 1 277 7 0.0 4 0 0 0 0 7 278 3 0.0 4 0 0 0 0 3 279 2 0.0 4 0 0 0 1 1 280 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 2,416 Reads with adapters: 366 (15.1%) Reads written (passing filters): 2,416 (100.0%) Total basepairs processed: 627,548 bp Total written (filtered): 623,539 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 366 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 1.9% G: 94.3% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 10 19 0.0 1 0 19 11 346 0.0 1 1 345 13 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.