This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 105.11 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 567,416 (17.8%) Reads that were too short: 146,023 (4.6%) Reads written (passing filters): 421,393 (13.2%) Total basepairs processed: 958,672,960 bp Total written (filtered): 95,849,252 bp (10.0%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 567416 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1155 777.6 0 1155 7 1496 777.6 0 1496 8 4148 777.6 0 4148 9 218881 777.6 0 218881 10 310 777.6 0 310 11 23 777.6 0 23 12 13 777.6 0 13 13 5 777.6 0 5 14 4 777.6 0 4 15 24 777.6 0 24 16 13 777.6 0 13 17 10 777.6 0 10 18 7 777.6 0 7 19 2 777.6 0 2 20 9 777.6 0 9 21 6 777.6 0 6 22 7 777.6 0 7 23 8 777.6 0 8 24 3 777.6 0 3 25 4 777.6 0 4 26 3 777.6 0 3 27 28 777.6 0 28 28 6 777.6 0 6 29 5 777.6 0 5 30 10 777.6 0 10 31 12 777.6 0 12 32 10 777.6 0 10 33 3 777.6 0 3 34 3 777.6 0 3 35 7 777.6 0 7 36 4 777.6 0 4 37 2 777.6 0 2 38 3 777.6 0 3 39 6 777.6 0 6 40 5 777.6 0 5 41 10 777.6 0 10 42 16 777.6 0 16 43 71 777.6 0 71 44 6 777.6 0 6 45 6 777.6 0 6 46 3 777.6 0 3 47 6 777.6 0 6 48 3 777.6 0 3 49 9 777.6 0 9 50 10 777.6 0 10 51 9 777.6 0 9 52 7 777.6 0 7 53 10 777.6 0 10 54 27 777.6 0 27 55 15 777.6 0 15 56 29 777.6 0 29 57 696 777.6 0 696 58 12 777.6 0 12 59 4 777.6 0 4 60 5 777.6 0 5 61 4 777.6 0 4 62 7 777.6 0 7 63 2 777.6 0 2 64 4 777.6 0 4 65 16 777.6 0 16 66 5 777.6 0 5 67 8 777.6 0 8 68 13 777.6 0 13 69 13 777.6 0 13 70 10 777.6 0 10 71 12 777.6 0 12 72 104 777.6 0 104 73 70 777.6 0 70 74 106 777.6 0 106 75 68 777.6 0 68 76 32 777.6 0 32 77 10 777.6 0 10 78 120 777.6 0 120 79 592 777.6 0 592 80 1968 777.6 0 1968 81 854 777.6 0 854 82 1612 777.6 0 1612 83 1388 777.6 0 1388 84 1408 777.6 0 1408 85 2248 777.6 0 2248 86 1337 777.6 0 1337 87 660 777.6 0 660 88 1809 777.6 0 1809 89 528 777.6 0 528 90 1635 777.6 0 1635 91 417 777.6 0 417 92 707 777.6 0 707 93 856 777.6 0 856 94 2099 777.6 0 2099 95 1013 777.6 0 1013 96 740 777.6 0 740 97 327 777.6 0 327 98 346 777.6 0 346 99 466 777.6 0 466 100 2999 777.6 0 2999 101 1555 777.6 0 1555 102 1001 777.6 0 1001 103 907 777.6 0 907 104 975 777.6 0 975 105 535 777.6 0 535 106 1051 777.6 0 1051 107 968 777.6 0 968 108 365 777.6 0 365 109 608 777.6 0 608 110 1714 777.6 0 1714 111 903 777.6 0 903 112 911 777.6 0 911 113 647 777.6 0 647 114 780 777.6 0 780 115 495 777.6 0 495 116 1557 777.6 0 1557 117 955 777.6 0 955 118 2091 777.6 0 2091 119 2448 777.6 0 2448 120 2499 777.6 0 2499 121 1488 777.6 0 1488 122 1592 777.6 0 1592 123 1314 777.6 0 1314 124 1078 777.6 0 1078 125 1440 777.6 0 1440 126 1021 777.6 0 1021 127 927 777.6 0 927 128 1030 777.6 0 1030 129 959 777.6 0 959 130 1001 777.6 0 1001 131 2591 777.6 0 2591 132 1165 777.6 0 1165 133 1206 777.6 0 1206 134 930 777.6 0 930 135 1039 777.6 0 1039 136 1354 777.6 0 1354 137 1364 777.6 0 1364 138 1645 777.6 0 1645 139 1422 777.6 0 1422 140 1501 777.6 0 1501 141 932 777.6 0 932 142 1166 777.6 0 1166 143 919 777.6 0 919 144 986 777.6 0 986 145 1901 777.6 0 1901 146 1473 777.6 0 1473 147 2015 777.6 0 2015 148 1048 777.6 0 1048 149 1231 777.6 0 1231 150 897 777.6 0 897 151 992 777.6 0 992 152 1668 777.6 0 1668 153 2208 777.6 0 2208 154 1412 777.6 0 1412 155 1293 777.6 0 1293 156 1022 777.6 0 1022 157 1695 777.6 0 1695 158 1701 777.6 0 1701 159 1263 777.6 0 1263 160 1552 777.6 0 1552 161 960 777.6 0 960 162 1414 777.6 0 1414 163 1085 777.6 0 1085 164 1292 777.6 0 1292 165 1163 777.6 0 1163 166 4260 777.6 0 4260 167 1915 777.6 0 1915 168 2770 777.6 0 2770 169 1483 777.6 0 1483 170 1565 777.6 0 1565 171 3803 777.6 0 3803 172 1293 777.6 0 1293 173 1603 777.6 0 1603 174 1162 777.6 0 1162 175 1204 777.6 0 1204 176 1014 777.6 0 1014 177 1338 777.6 0 1338 178 1538 777.6 0 1538 179 1863 777.6 0 1863 180 7666 777.6 0 7666 181 15677 777.6 0 15677 182 3760 777.6 0 3760 183 6354 777.6 0 6354 184 2119 777.6 0 2119 185 1927 777.6 0 1927 186 1181 777.6 0 1181 187 1273 777.6 0 1273 188 1042 777.6 0 1042 189 1339 777.6 0 1339 190 1012 777.6 0 1012 191 1322 777.6 0 1322 192 1149 777.6 0 1149 193 1030 777.6 0 1030 194 722 777.6 0 722 195 1019 777.6 0 1019 196 1026 777.6 0 1026 197 1233 777.6 0 1233 198 2132 777.6 0 2132 199 2297 777.6 0 2297 200 1905 777.6 0 1905 201 1275 777.6 0 1275 202 939 777.6 0 939 203 1343 777.6 0 1343 204 1555 777.6 0 1555 205 1595 777.6 0 1595 206 1177 777.6 0 1177 207 1251 777.6 0 1251 208 844 777.6 0 844 209 964 777.6 0 964 210 1255 777.6 0 1255 211 1160 777.6 0 1160 212 1250 777.6 0 1250 213 2134 777.6 0 2134 214 2823 777.6 0 2823 215 2247 777.6 0 2247 216 1171 777.6 0 1171 217 1080 777.6 0 1080 218 1682 777.6 0 1682 219 1526 777.6 0 1526 220 881 777.6 0 881 221 851 777.6 0 851 222 927 777.6 0 927 223 1822 777.6 0 1822 224 1340 777.6 0 1340 225 829 777.6 0 829 226 845 777.6 0 845 227 811 777.6 0 811 228 1027 777.6 0 1027 229 1045 777.6 0 1045 230 964 777.6 0 964 231 948 777.6 0 948 232 827 777.6 0 827 233 1130 777.6 0 1130 234 1180 777.6 0 1180 235 1455 777.6 0 1455 236 959 777.6 0 959 237 1369 777.6 0 1369 238 1109 777.6 0 1109 239 1354 777.6 0 1354 240 1219 777.6 0 1219 241 1062 777.6 0 1062 242 1638 777.6 0 1638 243 1295 777.6 0 1295 244 1561 777.6 0 1561 245 958 777.6 0 958 246 1053 777.6 0 1053 247 909 777.6 0 909 248 1269 777.6 0 1269 249 865 777.6 0 865 250 1248 777.6 0 1248 251 1006 777.6 0 1006 252 1623 777.6 0 1623 253 1500 777.6 0 1500 254 1114 777.6 0 1114 255 817 777.6 0 817 256 900 777.6 0 900 257 943 777.6 0 943 258 962 777.6 0 962 259 726 777.6 0 726 260 851 777.6 0 851 261 1302 777.6 0 1302 262 1508 777.6 0 1508 263 1865 777.6 0 1865 264 1372 777.6 0 1372 265 888 777.6 0 888 266 1298 777.6 0 1298 267 5138 777.6 0 5138 268 5408 777.6 0 5408 269 17136 777.6 0 17136 270 1397 777.6 0 1397 271 1025 777.6 0 1025 272 1400 777.6 0 1400 273 1450 777.6 0 1450 274 1189 777.6 0 1189 275 848 777.6 0 848 276 855 777.6 0 855 277 870 777.6 0 870 278 1172 777.6 0 1172 279 1329 777.6 0 1329 280 1031 777.6 0 1031 281 1448 777.6 0 1448 282 892 777.6 0 892 283 942 777.6 0 942 284 966 777.6 0 966 285 828 777.6 0 828 286 937 777.6 0 937 287 771 777.6 0 771 288 827 777.6 0 827 289 672 777.6 0 672 290 982 777.6 0 982 291 1512 777.6 0 1512 292 1904 777.6 0 1904 293 1484 777.6 0 1484 294 1026 777.6 0 1026 295 612 777.6 0 612 296 961 777.6 0 961 297 1450 777.6 0 1450 298 1722 777.6 0 1722 299 1874 777.6 0 1874 300 2215 777.6 0 2215 301 2329 777.6 0 2329 Finished in 17.84 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 421,393 Reads with adapters: 225,220 (53.4%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 225,189 (53.4%) Total basepairs processed: 95,849,252 bp Total written (filtered): 61,926,556 bp (64.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 225220 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 82 0.0 3 0 0 79 3 16 1400 0.0 3 0 1368 28 4 17 223000 0.0 3 217862 4957 154 27 18 448 0.0 3 18 406 17 7 19 112 0.0 3 1 0 96 15 20 69 0.0 3 0 0 0 69 21 6 0.0 3 1 0 0 5 22 3 0.0 3 2 0 0 1 23 1 0.0 3 0 0 0 1 24 32 0.0 3 1 0 1 30 28 2 0.0 3 2 36 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 1 2 144 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 10.20 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 225,189 Reads with adapters: 8,493 (3.8%) Reads written (passing filters): 225,189 (100.0%) Total basepairs processed: 61,926,556 bp Total written (filtered): 61,901,044 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 8493 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.3% C: 8.0% G: 53.8% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 8461 3518.6 0 8461 4 31 879.6 0 31 5 1 219.9 0 1