This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.85 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 55,830 (1.8%) Reads that were too short: 3,902 (0.1%) Reads written (passing filters): 51,928 (1.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 12,232,633 bp (1.3%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 55830 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 27 777.6 0 27 7 75 777.6 0 75 8 1423 777.6 0 1423 9 62 777.6 0 62 10 10 777.6 0 10 11 5 777.6 0 5 12 10 777.6 0 10 13 11 777.6 0 11 14 9 777.6 0 9 15 6 777.6 0 6 16 11 777.6 0 11 17 6 777.6 0 6 18 12 777.6 0 12 19 10 777.6 0 10 20 12 777.6 0 12 21 9 777.6 0 9 22 11 777.6 0 11 23 8 777.6 0 8 24 10 777.6 0 10 25 8 777.6 0 8 26 10 777.6 0 10 27 10 777.6 0 10 28 5 777.6 0 5 29 5 777.6 0 5 30 36 777.6 0 36 31 72 777.6 0 72 32 7 777.6 0 7 33 8 777.6 0 8 34 7 777.6 0 7 35 8 777.6 0 8 36 9 777.6 0 9 37 10 777.6 0 10 38 13 777.6 0 13 39 99 777.6 0 99 40 9 777.6 0 9 41 8 777.6 0 8 42 15 777.6 0 15 43 11 777.6 0 11 44 11 777.6 0 11 45 4 777.6 0 4 46 7 777.6 0 7 47 5 777.6 0 5 48 3 777.6 0 3 49 9 777.6 0 9 50 9 777.6 0 9 51 14 777.6 0 14 52 7 777.6 0 7 53 29 777.6 0 29 54 62 777.6 0 62 55 122 777.6 0 122 56 41960 777.6 0 41960 57 30 777.6 0 30 58 15 777.6 0 15 59 11 777.6 0 11 60 8 777.6 0 8 61 15 777.6 0 15 62 11 777.6 0 11 63 9 777.6 0 9 64 13 777.6 0 13 65 10 777.6 0 10 66 9 777.6 0 9 67 32 777.6 0 32 68 24 777.6 0 24 69 38 777.6 0 38 70 20 777.6 0 20 71 58 777.6 0 58 72 316 777.6 0 316 73 640 777.6 0 640 74 553 777.6 0 553 75 718 777.6 0 718 76 14 777.6 0 14 77 4 777.6 0 4 78 11 777.6 0 11 79 11 777.6 0 11 80 5 777.6 0 5 81 6 777.6 0 6 82 8 777.6 0 8 83 7 777.6 0 7 84 10 777.6 0 10 85 5 777.6 0 5 86 11 777.6 0 11 87 12 777.6 0 12 88 9 777.6 0 9 89 6 777.6 0 6 90 8 777.6 0 8 91 4 777.6 0 4 92 8 777.6 0 8 93 5 777.6 0 5 94 4 777.6 0 4 95 9 777.6 0 9 96 2 777.6 0 2 97 3 777.6 0 3 98 6 777.6 0 6 99 6 777.6 0 6 100 4 777.6 0 4 101 8 777.6 0 8 102 11 777.6 0 11 103 9 777.6 0 9 104 9 777.6 0 9 105 6 777.6 0 6 106 10 777.6 0 10 107 3 777.6 0 3 108 12 777.6 0 12 109 6 777.6 0 6 110 12 777.6 0 12 111 4 777.6 0 4 112 5 777.6 0 5 113 7 777.6 0 7 114 4 777.6 0 4 115 11 777.6 0 11 116 16 777.6 0 16 117 23 777.6 0 23 118 11 777.6 0 11 119 8 777.6 0 8 120 10 777.6 0 10 121 11 777.6 0 11 122 12 777.6 0 12 123 18 777.6 0 18 124 10 777.6 0 10 125 5 777.6 0 5 126 5 777.6 0 5 127 10 777.6 0 10 128 11 777.6 0 11 129 10 777.6 0 10 130 16 777.6 0 16 131 21 777.6 0 21 132 15 777.6 0 15 133 24 777.6 0 24 134 17 777.6 0 17 135 30 777.6 0 30 136 132 777.6 0 132 137 47 777.6 0 47 138 30 777.6 0 30 139 16 777.6 0 16 140 16 777.6 0 16 141 14 777.6 0 14 142 19 777.6 0 19 143 10 777.6 0 10 144 7 777.6 0 7 145 28 777.6 0 28 146 489 777.6 0 489 147 59 777.6 0 59 148 113 777.6 0 113 149 79 777.6 0 79 150 10 777.6 0 10 151 7 777.6 0 7 152 8 777.6 0 8 153 10 777.6 0 10 154 10 777.6 0 10 155 19 777.6 0 19 156 29 777.6 0 29 157 56 777.6 0 56 158 232 777.6 0 232 159 123 777.6 0 123 160 162 777.6 0 162 161 18 777.6 0 18 162 43 777.6 0 43 163 19 777.6 0 19 164 15 777.6 0 15 165 14 777.6 0 14 166 31 777.6 0 31 167 48 777.6 0 48 168 43 777.6 0 43 169 27 777.6 0 27 170 35 777.6 0 35 171 41 777.6 0 41 172 16 777.6 0 16 173 32 777.6 0 32 174 347 777.6 0 347 175 133 777.6 0 133 176 108 777.6 0 108 177 11 777.6 0 11 178 20 777.6 0 20 179 14 777.6 0 14 180 103 777.6 0 103 181 368 777.6 0 368 182 259 777.6 0 259 183 36 777.6 0 36 184 31 777.6 0 31 185 45 777.6 0 45 186 462 777.6 0 462 187 167 777.6 0 167 188 134 777.6 0 134 189 50 777.6 0 50 190 21 777.6 0 21 191 11 777.6 0 11 192 8 777.6 0 8 193 8 777.6 0 8 194 4 777.6 0 4 195 9 777.6 0 9 196 6 777.6 0 6 197 9 777.6 0 9 198 9 777.6 0 9 199 5 777.6 0 5 200 9 777.6 0 9 201 7 777.6 0 7 202 6 777.6 0 6 203 12 777.6 0 12 204 49 777.6 0 49 205 32 777.6 0 32 206 24 777.6 0 24 207 8 777.6 0 8 208 9 777.6 0 9 209 12 777.6 0 12 210 12 777.6 0 12 211 91 777.6 0 91 212 9 777.6 0 9 213 6 777.6 0 6 214 23 777.6 0 23 215 4 777.6 0 4 216 19 777.6 0 19 217 23 777.6 0 23 218 8 777.6 0 8 219 12 777.6 0 12 220 7 777.6 0 7 221 9 777.6 0 9 222 9 777.6 0 9 223 12 777.6 0 12 224 11 777.6 0 11 225 24 777.6 0 24 226 43 777.6 0 43 227 174 777.6 0 174 228 35 777.6 0 35 229 65 777.6 0 65 230 17 777.6 0 17 231 9 777.6 0 9 232 8 777.6 0 8 233 15 777.6 0 15 234 3 777.6 0 3 235 8 777.6 0 8 236 23 777.6 0 23 237 25 777.6 0 25 238 16 777.6 0 16 239 12 777.6 0 12 240 11 777.6 0 11 241 11 777.6 0 11 242 20 777.6 0 20 243 4 777.6 0 4 244 12 777.6 0 12 245 13 777.6 0 13 246 26 777.6 0 26 247 18 777.6 0 18 248 14 777.6 0 14 249 30 777.6 0 30 250 36 777.6 0 36 251 206 777.6 0 206 252 250 777.6 0 250 253 1147 777.6 0 1147 254 53 777.6 0 53 255 34 777.6 0 34 256 17 777.6 0 17 257 32 777.6 0 32 258 9 777.6 0 9 259 28 777.6 0 28 260 17 777.6 0 17 261 30 777.6 0 30 262 41 777.6 0 41 263 67 777.6 0 67 264 30 777.6 0 30 265 31 777.6 0 31 266 6 777.6 0 6 267 18 777.6 0 18 268 11 777.6 0 11 269 11 777.6 0 11 270 7 777.6 0 7 271 1 777.6 0 1 272 17 777.6 0 17 273 15 777.6 0 15 274 18 777.6 0 18 275 19 777.6 0 19 276 20 777.6 0 20 277 14 777.6 0 14 278 21 777.6 0 21 279 16 777.6 0 16 280 23 777.6 0 23 281 29 777.6 0 29 282 137 777.6 0 137 283 17 777.6 0 17 284 15 777.6 0 15 285 80 777.6 0 80 286 20 777.6 0 20 287 8 777.6 0 8 288 10 777.6 0 10 289 7 777.6 0 7 290 3 777.6 0 3 291 7 777.6 0 7 292 12 777.6 0 12 293 21 777.6 0 21 294 19 777.6 0 19 295 60 777.6 0 60 296 10 777.6 0 10 297 19 777.6 0 19 298 13 777.6 0 13 299 28 777.6 0 28 300 60 777.6 0 60 301 39 777.6 0 39 Finished in 2.30 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 51,928 Reads with adapters: 1,470 (2.8%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 1,456 (2.8%) Total basepairs processed: 12,232,633 bp Total written (filtered): 398,918 bp (3.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1470 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 15 0.0 3 0 15 17 1413 0.0 3 1389 23 0 1 18 4 0.0 3 0 4 19 3 0.0 3 0 0 2 1 20 1 0.0 3 0 0 0 1 27 1 0.0 3 1 33 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 63 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 106 6 0.0 3 0 0 0 6 107 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 1,456 Reads with adapters: 22 (1.5%) Reads written (passing filters): 1,456 (100.0%) Total basepairs processed: 398,918 bp Total written (filtered): 398,852 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 22 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.9% C: 31.8% G: 27.3% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 22 22.8 0 22