This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 105.16 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 373,781 (11.7%) Reads that were too short: 7,622 (0.2%) Reads written (passing filters): 366,159 (11.5%) Total basepairs processed: 958,672,960 bp Total written (filtered): 69,042,129 bp (7.2%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 373781 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 101 777.6 0 101 7 134 777.6 0 134 8 4018 777.6 0 4018 9 7112 777.6 0 7112 10 21 777.6 0 21 11 22 777.6 0 22 12 18 777.6 0 18 13 24 777.6 0 24 14 25 777.6 0 25 15 43 777.6 0 43 16 17 777.6 0 17 17 43 777.6 0 43 18 33 777.6 0 33 19 58 777.6 0 58 20 16 777.6 0 16 21 18 777.6 0 18 22 16 777.6 0 16 23 24 777.6 0 24 24 15 777.6 0 15 25 122 777.6 0 122 26 299 777.6 0 299 27 110 777.6 0 110 28 13 777.6 0 13 29 17 777.6 0 17 30 17 777.6 0 17 31 18 777.6 0 18 32 38 777.6 0 38 33 15 777.6 0 15 34 24 777.6 0 24 35 49 777.6 0 49 36 64 777.6 0 64 37 3352 777.6 0 3352 38 174 777.6 0 174 39 20 777.6 0 20 40 21 777.6 0 21 41 9 777.6 0 9 42 19 777.6 0 19 43 12 777.6 0 12 44 23 777.6 0 23 45 14 777.6 0 14 46 15 777.6 0 15 47 22 777.6 0 22 48 11 777.6 0 11 49 6 777.6 0 6 50 13 777.6 0 13 51 16 777.6 0 16 52 16 777.6 0 16 53 12 777.6 0 12 54 17 777.6 0 17 55 31 777.6 0 31 56 63 777.6 0 63 57 21 777.6 0 21 58 60 777.6 0 60 59 14 777.6 0 14 60 22 777.6 0 22 61 28 777.6 0 28 62 13 777.6 0 13 63 14 777.6 0 14 64 11 777.6 0 11 65 25 777.6 0 25 66 24 777.6 0 24 67 39 777.6 0 39 68 35 777.6 0 35 69 45 777.6 0 45 70 461 777.6 0 461 71 307 777.6 0 307 72 870 777.6 0 870 73 24 777.6 0 24 74 13 777.6 0 13 75 17 777.6 0 17 76 63 777.6 0 63 77 50 777.6 0 50 78 50 777.6 0 50 79 66 777.6 0 66 80 73 777.6 0 73 81 56 777.6 0 56 82 432 777.6 0 432 83 469 777.6 0 469 84 330 777.6 0 330 85 73 777.6 0 73 86 501 777.6 0 501 87 42 777.6 0 42 88 30 777.6 0 30 89 52 777.6 0 52 90 31 777.6 0 31 91 45 777.6 0 45 92 130 777.6 0 130 93 169 777.6 0 169 94 79 777.6 0 79 95 225 777.6 0 225 96 513 777.6 0 513 97 363 777.6 0 363 98 470 777.6 0 470 99 435 777.6 0 435 100 221 777.6 0 221 101 371 777.6 0 371 102 223 777.6 0 223 103 328 777.6 0 328 104 103 777.6 0 103 105 144 777.6 0 144 106 281 777.6 0 281 107 376 777.6 0 376 108 740 777.6 0 740 109 1055 777.6 0 1055 110 1623 777.6 0 1623 111 3234 777.6 0 3234 112 4230 777.6 0 4230 113 127707 777.6 0 127707 114 76361 777.6 0 76361 115 70822 777.6 0 70822 116 427 777.6 0 427 117 117 777.6 0 117 118 179 777.6 0 179 119 86 777.6 0 86 120 110 777.6 0 110 121 265 777.6 0 265 122 425 777.6 0 425 123 127 777.6 0 127 124 177 777.6 0 177 125 271 777.6 0 271 126 141 777.6 0 141 127 213 777.6 0 213 128 190 777.6 0 190 129 245 777.6 0 245 130 365 777.6 0 365 131 247 777.6 0 247 132 318 777.6 0 318 133 655 777.6 0 655 134 7864 777.6 0 7864 135 23496 777.6 0 23496 136 4258 777.6 0 4258 137 8493 777.6 0 8493 138 118 777.6 0 118 139 191 777.6 0 191 140 263 777.6 0 263 141 70 777.6 0 70 142 31 777.6 0 31 143 252 777.6 0 252 144 123 777.6 0 123 145 150 777.6 0 150 146 41 777.6 0 41 147 41 777.6 0 41 148 75 777.6 0 75 149 42 777.6 0 42 150 57 777.6 0 57 151 38 777.6 0 38 152 39 777.6 0 39 153 47 777.6 0 47 154 54 777.6 0 54 155 53 777.6 0 53 156 44 777.6 0 44 157 50 777.6 0 50 158 225 777.6 0 225 159 207 777.6 0 207 160 175 777.6 0 175 161 55 777.6 0 55 162 42 777.6 0 42 163 39 777.6 0 39 164 78 777.6 0 78 165 64 777.6 0 64 166 64 777.6 0 64 167 58 777.6 0 58 168 83 777.6 0 83 169 32 777.6 0 32 170 41 777.6 0 41 171 30 777.6 0 30 172 34 777.6 0 34 173 55 777.6 0 55 174 15 777.6 0 15 175 16 777.6 0 16 176 27 777.6 0 27 177 144 777.6 0 144 178 99 777.6 0 99 179 1136 777.6 0 1136 180 869 777.6 0 869 181 41 777.6 0 41 182 270 777.6 0 270 183 42 777.6 0 42 184 70 777.6 0 70 185 65 777.6 0 65 186 29 777.6 0 29 187 13 777.6 0 13 188 39 777.6 0 39 189 49 777.6 0 49 190 73 777.6 0 73 191 56 777.6 0 56 192 39 777.6 0 39 193 26 777.6 0 26 194 26 777.6 0 26 195 25 777.6 0 25 196 43 777.6 0 43 197 67 777.6 0 67 198 35 777.6 0 35 199 18 777.6 0 18 200 26 777.6 0 26 201 52 777.6 0 52 202 78 777.6 0 78 203 123 777.6 0 123 204 80 777.6 0 80 205 112 777.6 0 112 206 105 777.6 0 105 207 58 777.6 0 58 208 47 777.6 0 47 209 94 777.6 0 94 210 58 777.6 0 58 211 91 777.6 0 91 212 73 777.6 0 73 213 59 777.6 0 59 214 82 777.6 0 82 215 99 777.6 0 99 216 120 777.6 0 120 217 98 777.6 0 98 218 41 777.6 0 41 219 25 777.6 0 25 220 30 777.6 0 30 221 40 777.6 0 40 222 46 777.6 0 46 223 137 777.6 0 137 224 122 777.6 0 122 225 186 777.6 0 186 226 153 777.6 0 153 227 70 777.6 0 70 228 49 777.6 0 49 229 42 777.6 0 42 230 25 777.6 0 25 231 25 777.6 0 25 232 29 777.6 0 29 233 47 777.6 0 47 234 123 777.6 0 123 235 80 777.6 0 80 236 43 777.6 0 43 237 31 777.6 0 31 238 22 777.6 0 22 239 48 777.6 0 48 240 23 777.6 0 23 241 53 777.6 0 53 242 47 777.6 0 47 243 72 777.6 0 72 244 105 777.6 0 105 245 96 777.6 0 96 246 62 777.6 0 62 247 32 777.6 0 32 248 30 777.6 0 30 249 16 777.6 0 16 250 68 777.6 0 68 251 70 777.6 0 70 252 82 777.6 0 82 253 98 777.6 0 98 254 117 777.6 0 117 255 96 777.6 0 96 256 35 777.6 0 35 257 27 777.6 0 27 258 42 777.6 0 42 259 22 777.6 0 22 260 31 777.6 0 31 261 19 777.6 0 19 262 40 777.6 0 40 263 66 777.6 0 66 264 56 777.6 0 56 265 126 777.6 0 126 266 66 777.6 0 66 267 41 777.6 0 41 268 28 777.6 0 28 269 63 777.6 0 63 270 169 777.6 0 169 271 198 777.6 0 198 272 132 777.6 0 132 273 556 777.6 0 556 274 50 777.6 0 50 275 45 777.6 0 45 276 39 777.6 0 39 277 45 777.6 0 45 278 90 777.6 0 90 279 68 777.6 0 68 280 84 777.6 0 84 281 172 777.6 0 172 282 252 777.6 0 252 283 66 777.6 0 66 284 48 777.6 0 48 285 40 777.6 0 40 286 39 777.6 0 39 287 26 777.6 0 26 288 39 777.6 0 39 289 29 777.6 0 29 290 106 777.6 0 106 291 117 777.6 0 117 292 135 777.6 0 135 293 65 777.6 0 65 294 45 777.6 0 45 295 56 777.6 0 56 296 66 777.6 0 66 297 79 777.6 0 79 298 113 777.6 0 113 299 62 777.6 0 62 300 62 777.6 0 62 301 9 777.6 0 9 Finished in 17.55 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 366,159 Reads with adapters: 7,411 (2.0%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 7,388 (2.0%) Total basepairs processed: 69,042,129 bp Total written (filtered): 2,007,958 bp (2.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7411 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 18 0.0 3 0 13 0 0 5 20 7271 0.0 4 7013 208 35 10 5 21 77 0.0 4 34 35 5 2 1 22 3 0.0 4 0 0 3 23 2 0.0 4 0 0 0 2 31 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 1 63 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 97 8 0.0 4 0 0 0 0 8 127 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 168 4 0.0 4 0 0 0 0 4 170 1 0.0 4 0 0 0 0 1 171 3 0.0 4 0 0 0 0 3 178 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 7,388 Reads with adapters: 535 (7.2%) Reads written (passing filters): 7,388 (100.0%) Total basepairs processed: 2,007,958 bp Total written (filtered): 1,987,753 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 535 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 1.7% G: 82.1% T: 12.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 5 1 7.2 0 1 8 1 0.1 0 1 9 253 0.0 0 10 243 10 116 0.0 1 4 112 11 100 0.0 1 0 100 12 2 0.0 1 0 2 13 2 0.0 1 0 2 150 3 0.0 1 0 3 258 1 0.0 1 1 266 55 0.0 1 52 3 267 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.