This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.41 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 70,788 (2.2%) Reads that were too short: 25,396 (0.8%) Reads written (passing filters): 45,392 (1.4%) Total basepairs processed: 958,672,960 bp Total written (filtered): 7,897,453 bp (0.8%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 70788 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 49 777.6 0 49 7 51 777.6 0 51 8 190 777.6 0 190 9 6836 777.6 0 6836 10 11 777.6 0 11 11 8 777.6 0 8 12 10 777.6 0 10 13 12 777.6 0 12 14 12 777.6 0 12 15 7 777.6 0 7 16 5 777.6 0 5 17 5 777.6 0 5 18 5 777.6 0 5 19 9 777.6 0 9 20 9 777.6 0 9 21 10 777.6 0 10 22 14 777.6 0 14 23 8 777.6 0 8 24 9 777.6 0 9 25 10 777.6 0 10 26 12 777.6 0 12 27 12 777.6 0 12 28 22 777.6 0 22 29 9 777.6 0 9 30 12 777.6 0 12 31 5 777.6 0 5 32 7 777.6 0 7 33 8 777.6 0 8 34 283 777.6 0 283 35 5 777.6 0 5 36 15 777.6 0 15 37 10 777.6 0 10 38 88 777.6 0 88 39 193 777.6 0 193 40 28 777.6 0 28 41 7 777.6 0 7 42 5 777.6 0 5 43 11 777.6 0 11 44 8 777.6 0 8 45 117 777.6 0 117 46 4 777.6 0 4 47 5 777.6 0 5 48 7 777.6 0 7 49 6 777.6 0 6 50 12 777.6 0 12 51 14 777.6 0 14 52 10 777.6 0 10 53 70 777.6 0 70 54 45 777.6 0 45 55 32 777.6 0 32 56 19 777.6 0 19 57 14 777.6 0 14 58 18 777.6 0 18 59 8 777.6 0 8 60 5 777.6 0 5 61 14 777.6 0 14 62 5 777.6 0 5 63 11 777.6 0 11 64 10 777.6 0 10 65 6 777.6 0 6 66 78 777.6 0 78 67 18 777.6 0 18 68 7 777.6 0 7 69 11 777.6 0 11 70 14 777.6 0 14 71 12 777.6 0 12 72 12 777.6 0 12 73 12 777.6 0 12 74 6 777.6 0 6 75 6 777.6 0 6 76 9 777.6 0 9 77 6 777.6 0 6 78 9 777.6 0 9 79 9 777.6 0 9 80 15 777.6 0 15 81 17 777.6 0 17 82 14 777.6 0 14 83 23 777.6 0 23 84 22 777.6 0 22 85 16 777.6 0 16 86 10 777.6 0 10 87 11 777.6 0 11 88 23 777.6 0 23 89 23 777.6 0 23 90 6 777.6 0 6 91 74 777.6 0 74 92 59 777.6 0 59 93 86 777.6 0 86 94 31 777.6 0 31 95 29 777.6 0 29 96 15 777.6 0 15 97 25 777.6 0 25 98 59 777.6 0 59 99 60 777.6 0 60 100 14 777.6 0 14 101 55 777.6 0 55 102 72 777.6 0 72 103 199 777.6 0 199 104 245 777.6 0 245 105 348 777.6 0 348 106 35 777.6 0 35 107 58 777.6 0 58 108 139 777.6 0 139 109 140 777.6 0 140 110 21 777.6 0 21 111 166 777.6 0 166 112 102 777.6 0 102 113 305 777.6 0 305 114 97 777.6 0 97 115 115 777.6 0 115 116 40 777.6 0 40 117 53 777.6 0 53 118 31 777.6 0 31 119 20 777.6 0 20 120 106 777.6 0 106 121 82 777.6 0 82 122 68 777.6 0 68 123 111 777.6 0 111 124 183 777.6 0 183 125 1228 777.6 0 1228 126 975 777.6 0 975 127 892 777.6 0 892 128 70 777.6 0 70 129 76 777.6 0 76 130 311 777.6 0 311 131 48 777.6 0 48 132 106 777.6 0 106 133 107 777.6 0 107 134 155 777.6 0 155 135 130 777.6 0 130 136 63 777.6 0 63 137 116 777.6 0 116 138 147 777.6 0 147 139 276 777.6 0 276 140 130 777.6 0 130 141 61 777.6 0 61 142 52 777.6 0 52 143 167 777.6 0 167 144 496 777.6 0 496 145 151 777.6 0 151 146 181 777.6 0 181 147 146 777.6 0 146 148 101 777.6 0 101 149 113 777.6 0 113 150 2233 777.6 0 2233 151 415 777.6 0 415 152 932 777.6 0 932 153 104 777.6 0 104 154 233 777.6 0 233 155 193 777.6 0 193 156 465 777.6 0 465 157 400 777.6 0 400 158 348 777.6 0 348 159 780 777.6 0 780 160 6755 777.6 0 6755 161 3090 777.6 0 3090 162 3917 777.6 0 3917 163 275 777.6 0 275 164 432 777.6 0 432 165 182 777.6 0 182 166 328 777.6 0 328 167 135 777.6 0 135 168 178 777.6 0 178 169 118 777.6 0 118 170 101 777.6 0 101 171 141 777.6 0 141 172 195 777.6 0 195 173 73 777.6 0 73 174 42 777.6 0 42 175 86 777.6 0 86 176 86 777.6 0 86 177 132 777.6 0 132 178 138 777.6 0 138 179 124 777.6 0 124 180 301 777.6 0 301 181 545 777.6 0 545 182 1510 777.6 0 1510 183 263 777.6 0 263 184 110 777.6 0 110 185 119 777.6 0 119 186 150 777.6 0 150 187 65 777.6 0 65 188 134 777.6 0 134 189 142 777.6 0 142 190 183 777.6 0 183 191 122 777.6 0 122 192 63 777.6 0 63 193 60 777.6 0 60 194 26 777.6 0 26 195 43 777.6 0 43 196 97 777.6 0 97 197 121 777.6 0 121 198 46 777.6 0 46 199 47 777.6 0 47 200 56 777.6 0 56 201 186 777.6 0 186 202 122 777.6 0 122 203 65 777.6 0 65 204 50 777.6 0 50 205 58 777.6 0 58 206 68 777.6 0 68 207 97 777.6 0 97 208 177 777.6 0 177 209 157 777.6 0 157 210 128 777.6 0 128 211 342 777.6 0 342 212 2078 777.6 0 2078 213 1090 777.6 0 1090 214 1377 777.6 0 1377 215 133 777.6 0 133 216 285 777.6 0 285 217 311 777.6 0 311 218 318 777.6 0 318 219 509 777.6 0 509 220 91 777.6 0 91 221 192 777.6 0 192 222 243 777.6 0 243 223 118 777.6 0 118 224 125 777.6 0 125 225 407 777.6 0 407 226 269 777.6 0 269 227 226 777.6 0 226 228 211 777.6 0 211 229 183 777.6 0 183 230 96 777.6 0 96 231 63 777.6 0 63 232 132 777.6 0 132 233 145 777.6 0 145 234 113 777.6 0 113 235 53 777.6 0 53 236 101 777.6 0 101 237 176 777.6 0 176 238 160 777.6 0 160 239 389 777.6 0 389 240 219 777.6 0 219 241 330 777.6 0 330 242 169 777.6 0 169 243 213 777.6 0 213 244 158 777.6 0 158 245 318 777.6 0 318 246 258 777.6 0 258 247 146 777.6 0 146 248 119 777.6 0 119 249 156 777.6 0 156 250 199 777.6 0 199 251 200 777.6 0 200 252 143 777.6 0 143 253 162 777.6 0 162 254 342 777.6 0 342 255 589 777.6 0 589 256 577 777.6 0 577 257 389 777.6 0 389 258 192 777.6 0 192 259 144 777.6 0 144 260 178 777.6 0 178 261 148 777.6 0 148 262 172 777.6 0 172 263 157 777.6 0 157 264 242 777.6 0 242 265 283 777.6 0 283 266 877 777.6 0 877 267 449 777.6 0 449 268 311 777.6 0 311 269 185 777.6 0 185 270 273 777.6 0 273 271 586 777.6 0 586 272 248 777.6 0 248 273 270 777.6 0 270 274 313 777.6 0 313 275 283 777.6 0 283 276 262 777.6 0 262 277 304 777.6 0 304 278 268 777.6 0 268 279 149 777.6 0 149 280 259 777.6 0 259 281 285 777.6 0 285 282 642 777.6 0 642 283 242 777.6 0 242 284 200 777.6 0 200 285 188 777.6 0 188 286 180 777.6 0 180 287 248 777.6 0 248 288 178 777.6 0 178 289 278 777.6 0 278 290 97 777.6 0 97 291 95 777.6 0 95 292 61 777.6 0 61 293 44 777.6 0 44 294 29 777.6 0 29 295 52 777.6 0 52 296 28 777.6 0 28 297 63 777.6 0 63 298 98 777.6 0 98 299 120 777.6 0 120 300 111 777.6 0 111 301 59 777.6 0 59 Finished in 1.85 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 45,392 Reads with adapters: 7,083 (15.6%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 7,073 (15.6%) Total basepairs processed: 7,897,453 bp Total written (filtered): 1,943,523 bp (24.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7083 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 53 0.0 3 0 52 1 17 6968 0.0 3 6778 183 6 1 18 24 0.0 3 0 23 0 1 19 11 0.0 3 0 0 7 4 20 3 0.0 3 0 0 0 3 21 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 134 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 165 3 0.0 3 0 0 0 3 241 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 7,073 Reads with adapters: 164 (2.3%) Reads written (passing filters): 7,073 (100.0%) Total basepairs processed: 1,943,523 bp Total written (filtered): 1,943,030 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 164 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 39.6% C: 15.9% G: 42.7% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 163 110.5 0 163 4 1 27.6 0 1