This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.14 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 75,685 (2.4%) Reads that were too short: 2,770 (0.1%) Reads written (passing filters): 72,915 (2.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 18,890,292 bp (2.0%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 75685 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 422 777.6 0 422 7 14144 777.6 0 14144 8 13782 777.6 0 13782 9 29 777.6 0 29 10 17 777.6 0 17 11 70 777.6 0 70 12 48 777.6 0 48 13 6 777.6 0 6 14 14 777.6 0 14 15 7 777.6 0 7 16 3 777.6 0 3 17 5 777.6 0 5 18 15 777.6 0 15 19 9 777.6 0 9 20 7 777.6 0 7 21 14 777.6 0 14 22 14 777.6 0 14 23 8 777.6 0 8 24 4 777.6 0 4 25 9 777.6 0 9 26 10 777.6 0 10 27 17 777.6 0 17 28 60 777.6 0 60 29 12 777.6 0 12 30 6 777.6 0 6 31 12 777.6 0 12 32 5 777.6 0 5 33 10 777.6 0 10 34 9 777.6 0 9 35 12 777.6 0 12 36 14 777.6 0 14 37 33 777.6 0 33 38 73 777.6 0 73 39 27 777.6 0 27 40 163 777.6 0 163 41 27 777.6 0 27 42 32 777.6 0 32 43 246 777.6 0 246 44 50 777.6 0 50 45 41 777.6 0 41 46 16 777.6 0 16 47 77 777.6 0 77 48 47 777.6 0 47 49 124 777.6 0 124 50 111 777.6 0 111 51 135 777.6 0 135 52 224 777.6 0 224 53 439 777.6 0 439 54 582 777.6 0 582 55 17287 777.6 0 17287 56 5734 777.6 0 5734 57 11032 777.6 0 11032 58 51 777.6 0 51 59 12 777.6 0 12 60 2 777.6 0 2 61 9 777.6 0 9 62 4 777.6 0 4 63 4 777.6 0 4 64 2 777.6 0 2 65 6 777.6 0 6 66 3 777.6 0 3 67 7 777.6 0 7 68 18 777.6 0 18 69 9 777.6 0 9 70 9 777.6 0 9 71 10 777.6 0 10 72 12 777.6 0 12 73 9 777.6 0 9 74 10 777.6 0 10 75 13 777.6 0 13 76 4 777.6 0 4 77 9 777.6 0 9 78 35 777.6 0 35 79 13 777.6 0 13 80 23 777.6 0 23 81 37 777.6 0 37 82 49 777.6 0 49 83 68 777.6 0 68 84 87 777.6 0 87 85 2408 777.6 0 2408 86 1088 777.6 0 1088 87 1037 777.6 0 1037 88 16 777.6 0 16 89 15 777.6 0 15 90 3 777.6 0 3 91 5 777.6 0 5 92 8 777.6 0 8 93 23 777.6 0 23 94 6 777.6 0 6 95 3 777.6 0 3 96 6 777.6 0 6 97 6 777.6 0 6 98 9 777.6 0 9 99 8 777.6 0 8 100 12 777.6 0 12 101 4 777.6 0 4 102 75 777.6 0 75 103 14 777.6 0 14 104 20 777.6 0 20 105 6 777.6 0 6 106 7 777.6 0 7 107 5 777.6 0 5 108 4 777.6 0 4 109 7 777.6 0 7 110 3 777.6 0 3 111 3 777.6 0 3 112 12 777.6 0 12 113 3 777.6 0 3 114 6 777.6 0 6 116 6 777.6 0 6 117 10 777.6 0 10 118 63 777.6 0 63 119 28 777.6 0 28 120 73 777.6 0 73 121 8 777.6 0 8 122 11 777.6 0 11 123 12 777.6 0 12 124 12 777.6 0 12 125 9 777.6 0 9 126 10 777.6 0 10 127 11 777.6 0 11 128 8 777.6 0 8 129 34 777.6 0 34 130 14 777.6 0 14 131 15 777.6 0 15 132 23 777.6 0 23 133 41 777.6 0 41 134 21 777.6 0 21 135 13 777.6 0 13 136 20 777.6 0 20 137 41 777.6 0 41 138 172 777.6 0 172 139 97 777.6 0 97 140 47 777.6 0 47 141 15 777.6 0 15 142 11 777.6 0 11 143 4 777.6 0 4 144 24 777.6 0 24 145 7 777.6 0 7 146 14 777.6 0 14 147 12 777.6 0 12 148 49 777.6 0 49 149 42 777.6 0 42 150 16 777.6 0 16 151 49 777.6 0 49 152 11 777.6 0 11 153 7 777.6 0 7 154 15 777.6 0 15 155 10 777.6 0 10 156 11 777.6 0 11 157 54 777.6 0 54 158 74 777.6 0 74 159 72 777.6 0 72 160 16 777.6 0 16 161 9 777.6 0 9 162 20 777.6 0 20 163 25 777.6 0 25 164 55 777.6 0 55 165 22 777.6 0 22 166 27 777.6 0 27 167 41 777.6 0 41 168 48 777.6 0 48 169 22 777.6 0 22 170 18 777.6 0 18 171 12 777.6 0 12 172 10 777.6 0 10 173 8 777.6 0 8 174 11 777.6 0 11 175 2 777.6 0 2 176 7 777.6 0 7 177 8 777.6 0 8 178 8 777.6 0 8 179 6 777.6 0 6 180 11 777.6 0 11 181 26 777.6 0 26 182 22 777.6 0 22 183 29 777.6 0 29 184 29 777.6 0 29 185 21 777.6 0 21 186 9 777.6 0 9 187 57 777.6 0 57 188 45 777.6 0 45 189 97 777.6 0 97 190 62 777.6 0 62 191 15 777.6 0 15 192 134 777.6 0 134 193 3 777.6 0 3 194 5 777.6 0 5 195 9 777.6 0 9 196 5 777.6 0 5 197 2 777.6 0 2 198 2 777.6 0 2 199 10 777.6 0 10 200 13 777.6 0 13 201 7 777.6 0 7 202 5 777.6 0 5 203 5 777.6 0 5 204 9 777.6 0 9 205 8 777.6 0 8 206 17 777.6 0 17 207 16 777.6 0 16 208 16 777.6 0 16 209 12 777.6 0 12 210 5 777.6 0 5 211 3 777.6 0 3 212 8 777.6 0 8 213 15 777.6 0 15 214 16 777.6 0 16 215 30 777.6 0 30 216 22 777.6 0 22 217 32 777.6 0 32 218 59 777.6 0 59 219 395 777.6 0 395 220 35 777.6 0 35 221 24 777.6 0 24 222 9 777.6 0 9 223 29 777.6 0 29 224 10 777.6 0 10 225 21 777.6 0 21 226 22 777.6 0 22 227 10 777.6 0 10 228 8 777.6 0 8 229 4 777.6 0 4 230 10 777.6 0 10 231 8 777.6 0 8 232 5 777.6 0 5 233 11 777.6 0 11 234 31 777.6 0 31 235 11 777.6 0 11 236 21 777.6 0 21 237 5 777.6 0 5 238 11 777.6 0 11 239 4 777.6 0 4 240 30 777.6 0 30 241 4 777.6 0 4 242 6 777.6 0 6 243 13 777.6 0 13 244 21 777.6 0 21 245 12 777.6 0 12 246 12 777.6 0 12 247 6 777.6 0 6 248 3 777.6 0 3 249 8 777.6 0 8 250 13 777.6 0 13 251 93 777.6 0 93 252 54 777.6 0 54 253 44 777.6 0 44 254 76 777.6 0 76 255 54 777.6 0 54 256 13 777.6 0 13 257 18 777.6 0 18 258 13 777.6 0 13 259 51 777.6 0 51 260 6 777.6 0 6 261 5 777.6 0 5 262 12 777.6 0 12 263 9 777.6 0 9 264 14 777.6 0 14 265 12 777.6 0 12 266 20 777.6 0 20 267 22 777.6 0 22 268 33 777.6 0 33 269 48 777.6 0 48 270 8 777.6 0 8 271 5 777.6 0 5 272 7 777.6 0 7 273 8 777.6 0 8 274 14 777.6 0 14 275 9 777.6 0 9 276 77 777.6 0 77 277 54 777.6 0 54 278 101 777.6 0 101 279 26 777.6 0 26 280 30 777.6 0 30 281 47 777.6 0 47 282 51 777.6 0 51 283 29 777.6 0 29 284 23 777.6 0 23 285 34 777.6 0 34 286 15 777.6 0 15 287 31 777.6 0 31 288 11 777.6 0 11 289 32 777.6 0 32 290 15 777.6 0 15 291 47 777.6 0 47 292 32 777.6 0 32 293 15 777.6 0 15 294 16 777.6 0 16 295 27 777.6 0 27 296 75 777.6 0 75 297 59 777.6 0 59 298 58 777.6 0 58 299 41 777.6 0 41 300 57 777.6 0 57 301 29 777.6 0 29 Finished in 4.20 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 72,915 Reads with adapters: 14,271 (19.6%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 14,263 (19.6%) Total basepairs processed: 18,890,292 bp Total written (filtered): 3,890,527 bp (20.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14271 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 3 1 19 73 0.0 3 0 60 3 8 2 20 14062 0.0 4 13566 431 48 14 3 21 32 0.0 4 5 27 22 10 0.0 4 3 0 5 1 1 23 48 0.0 4 36 3 1 5 3 24 1 0.0 4 0 0 0 0 1 27 1 0.0 4 1 30 1 0.0 4 1 36 1 0.0 4 1 108 1 0.0 4 0 0 0 0 1 123 11 0.0 4 0 0 0 1 10 124 2 0.0 4 0 0 0 0 2 127 16 0.0 4 0 0 0 0 16 170 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.62 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 14,263 Reads with adapters: 1,670 (11.7%) Reads written (passing filters): 14,263 (100.0%) Total basepairs processed: 3,890,527 bp Total written (filtered): 3,871,535 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1670 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 2.8% G: 93.7% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 55.7 0 1 10 31 0.0 1 1 30 11 1632 0.0 1 7 1625 12 1 0.0 1 0 1 14 2 0.0 1 0 2 152 1 0.0 1 0 1 267 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.