This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.06 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 90,093 (2.8%) Reads that were too short: 16,405 (0.5%) Reads written (passing filters): 73,688 (2.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 20,248,677 bp (2.1%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 90093 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1305 777.6 0 1305 7 4043 777.6 0 4043 8 1254 777.6 0 1254 9 56130 777.6 0 56130 10 81 777.6 0 81 11 390 777.6 0 390 12 19 777.6 0 19 13 1 777.6 0 1 14 4 777.6 0 4 15 3 777.6 0 3 16 4 777.6 0 4 17 5 777.6 0 5 18 3 777.6 0 3 19 3 777.6 0 3 20 1 777.6 0 1 21 1 777.6 0 1 22 4 777.6 0 4 23 3 777.6 0 3 24 9 777.6 0 9 25 2 777.6 0 2 26 6 777.6 0 6 27 5 777.6 0 5 28 2 777.6 0 2 29 7 777.6 0 7 30 5 777.6 0 5 31 5 777.6 0 5 32 5 777.6 0 5 33 2 777.6 0 2 34 4 777.6 0 4 35 2 777.6 0 2 36 2 777.6 0 2 37 10 777.6 0 10 38 4 777.6 0 4 39 198 777.6 0 198 40 10 777.6 0 10 41 3 777.6 0 3 42 7 777.6 0 7 43 8 777.6 0 8 44 6 777.6 0 6 45 11 777.6 0 11 46 3 777.6 0 3 47 4 777.6 0 4 48 4 777.6 0 4 49 16 777.6 0 16 50 4 777.6 0 4 51 9 777.6 0 9 52 2 777.6 0 2 53 10 777.6 0 10 54 11 777.6 0 11 55 4 777.6 0 4 56 14 777.6 0 14 57 2 777.6 0 2 58 6 777.6 0 6 59 18 777.6 0 18 60 11 777.6 0 11 61 44 777.6 0 44 62 71 777.6 0 71 63 70 777.6 0 70 64 87 777.6 0 87 65 3 777.6 0 3 66 13 777.6 0 13 67 12 777.6 0 12 68 9 777.6 0 9 69 9 777.6 0 9 70 13 777.6 0 13 71 11 777.6 0 11 72 9 777.6 0 9 73 10 777.6 0 10 74 11 777.6 0 11 75 17 777.6 0 17 76 33 777.6 0 33 77 35 777.6 0 35 78 595 777.6 0 595 79 517 777.6 0 517 80 496 777.6 0 496 81 588 777.6 0 588 82 125 777.6 0 125 83 92 777.6 0 92 84 7 777.6 0 7 85 5 777.6 0 5 86 7 777.6 0 7 87 6 777.6 0 6 88 7 777.6 0 7 89 5 777.6 0 5 90 6 777.6 0 6 91 5 777.6 0 5 92 15 777.6 0 15 93 10 777.6 0 10 94 15 777.6 0 15 95 12 777.6 0 12 96 11 777.6 0 11 97 12 777.6 0 12 98 13 777.6 0 13 99 15 777.6 0 15 100 33 777.6 0 33 101 16 777.6 0 16 102 21 777.6 0 21 103 13 777.6 0 13 104 23 777.6 0 23 105 4 777.6 0 4 106 4 777.6 0 4 107 10 777.6 0 10 108 13 777.6 0 13 109 39 777.6 0 39 110 25 777.6 0 25 111 11 777.6 0 11 112 16 777.6 0 16 113 14 777.6 0 14 114 18 777.6 0 18 115 14 777.6 0 14 116 699 777.6 0 699 117 147 777.6 0 147 118 158 777.6 0 158 119 28 777.6 0 28 120 20 777.6 0 20 121 24 777.6 0 24 122 51 777.6 0 51 123 37 777.6 0 37 124 27 777.6 0 27 125 28 777.6 0 28 126 15 777.6 0 15 127 31 777.6 0 31 128 47 777.6 0 47 129 20 777.6 0 20 130 34 777.6 0 34 131 41 777.6 0 41 132 27 777.6 0 27 133 27 777.6 0 27 134 18 777.6 0 18 135 41 777.6 0 41 136 60 777.6 0 60 137 66 777.6 0 66 138 55 777.6 0 55 139 38 777.6 0 38 140 34 777.6 0 34 141 84 777.6 0 84 142 59 777.6 0 59 143 35 777.6 0 35 144 33 777.6 0 33 145 30 777.6 0 30 146 25 777.6 0 25 147 40 777.6 0 40 148 13 777.6 0 13 149 19 777.6 0 19 150 25 777.6 0 25 151 34 777.6 0 34 152 23 777.6 0 23 153 33 777.6 0 33 154 27 777.6 0 27 155 52 777.6 0 52 156 41 777.6 0 41 157 57 777.6 0 57 158 50 777.6 0 50 159 46 777.6 0 46 160 46 777.6 0 46 161 45 777.6 0 45 162 79 777.6 0 79 163 52 777.6 0 52 164 62 777.6 0 62 165 43 777.6 0 43 166 60 777.6 0 60 167 74 777.6 0 74 168 107 777.6 0 107 169 38 777.6 0 38 170 112 777.6 0 112 171 63 777.6 0 63 172 87 777.6 0 87 173 54 777.6 0 54 174 17 777.6 0 17 175 31 777.6 0 31 176 31 777.6 0 31 177 75 777.6 0 75 178 74 777.6 0 74 179 73 777.6 0 73 180 95 777.6 0 95 181 902 777.6 0 902 182 119 777.6 0 119 183 430 777.6 0 430 184 62 777.6 0 62 185 59 777.6 0 59 186 68 777.6 0 68 187 132 777.6 0 132 188 75 777.6 0 75 189 54 777.6 0 54 190 331 777.6 0 331 191 128 777.6 0 128 192 90 777.6 0 90 193 37 777.6 0 37 194 24 777.6 0 24 195 54 777.6 0 54 196 45 777.6 0 45 197 127 777.6 0 127 198 46 777.6 0 46 199 34 777.6 0 34 200 34 777.6 0 34 201 40 777.6 0 40 202 60 777.6 0 60 203 68 777.6 0 68 204 94 777.6 0 94 205 70 777.6 0 70 206 70 777.6 0 70 207 49 777.6 0 49 208 31 777.6 0 31 209 72 777.6 0 72 210 104 777.6 0 104 211 65 777.6 0 65 212 62 777.6 0 62 213 78 777.6 0 78 214 98 777.6 0 98 215 68 777.6 0 68 216 77 777.6 0 77 217 216 777.6 0 216 218 147 777.6 0 147 219 108 777.6 0 108 220 51 777.6 0 51 221 118 777.6 0 118 222 78 777.6 0 78 223 88 777.6 0 88 224 52 777.6 0 52 225 309 777.6 0 309 226 542 777.6 0 542 227 584 777.6 0 584 228 149 777.6 0 149 229 89 777.6 0 89 230 66 777.6 0 66 231 38 777.6 0 38 232 56 777.6 0 56 233 53 777.6 0 53 234 71 777.6 0 71 235 81 777.6 0 81 236 63 777.6 0 63 237 220 777.6 0 220 238 234 777.6 0 234 239 88 777.6 0 88 240 44 777.6 0 44 241 59 777.6 0 59 242 43 777.6 0 43 243 47 777.6 0 47 244 50 777.6 0 50 245 75 777.6 0 75 246 77 777.6 0 77 247 108 777.6 0 108 248 71 777.6 0 71 249 105 777.6 0 105 250 106 777.6 0 106 251 49 777.6 0 49 252 71 777.6 0 71 253 64 777.6 0 64 254 65 777.6 0 65 255 94 777.6 0 94 256 71 777.6 0 71 257 51 777.6 0 51 258 81 777.6 0 81 259 87 777.6 0 87 260 723 777.6 0 723 261 638 777.6 0 638 262 829 777.6 0 829 263 488 777.6 0 488 264 191 777.6 0 191 265 352 777.6 0 352 266 126 777.6 0 126 267 88 777.6 0 88 268 90 777.6 0 90 269 77 777.6 0 77 270 112 777.6 0 112 271 130 777.6 0 130 272 160 777.6 0 160 273 98 777.6 0 98 274 74 777.6 0 74 275 56 777.6 0 56 276 110 777.6 0 110 277 122 777.6 0 122 278 135 777.6 0 135 279 139 777.6 0 139 280 109 777.6 0 109 281 137 777.6 0 137 282 152 777.6 0 152 283 205 777.6 0 205 284 145 777.6 0 145 285 1744 777.6 0 1744 286 436 777.6 0 436 287 1098 777.6 0 1098 288 160 777.6 0 160 289 131 777.6 0 131 290 191 777.6 0 191 291 142 777.6 0 142 292 75 777.6 0 75 293 30 777.6 0 30 294 100 777.6 0 100 295 99 777.6 0 99 296 209 777.6 0 209 297 182 777.6 0 182 298 154 777.6 0 154 299 85 777.6 0 85 300 58 777.6 0 58 301 40 777.6 0 40 Finished in 3.57 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 73,688 Reads with adapters: 58,287 (79.1%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 58,276 (79.1%) Total basepairs processed: 20,248,677 bp Total written (filtered): 16,027,977 bp (79.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58287 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 6 0.0 3 0 0 6 16 228 0.0 3 0 218 9 1 17 57826 0.0 3 56505 1280 38 3 18 139 0.0 3 6 124 7 2 19 27 0.0 3 1 0 17 9 20 37 0.0 3 0 0 0 37 31 1 0.0 3 1 35 1 0.0 3 0 0 0 1 38 1 0.0 3 1 50 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 220 5 0.0 3 0 0 0 5 244 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.70 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 58,276 Reads with adapters: 6,193 (10.6%) Reads written (passing filters): 58,276 (100.0%) Total basepairs processed: 16,027,977 bp Total written (filtered): 16,009,376 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6193 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.3% C: 5.8% G: 40.4% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 6174 910.6 0 6174 4 17 227.6 0 17 5 1 56.9 0 1 6 1 14.2 0 1