This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.61 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 69,288 (2.2%) Reads that were too short: 4,810 (0.2%) Reads written (passing filters): 64,478 (2.0%) Total basepairs processed: 958,672,960 bp Total written (filtered): 18,145,828 bp (1.9%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 69288 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4675 777.6 0 4675 7 1005 777.6 0 1005 8 9831 777.6 0 9831 9 41983 777.6 0 41983 10 51 777.6 0 51 11 11 777.6 0 11 12 10 777.6 0 10 13 3 777.6 0 3 14 3 777.6 0 3 15 12 777.6 0 12 16 2 777.6 0 2 17 3 777.6 0 3 18 3 777.6 0 3 19 10 777.6 0 10 20 10 777.6 0 10 21 9 777.6 0 9 22 7 777.6 0 7 23 6 777.6 0 6 24 5 777.6 0 5 25 10 777.6 0 10 26 3 777.6 0 3 27 7 777.6 0 7 28 2 777.6 0 2 29 10 777.6 0 10 30 4 777.6 0 4 31 4 777.6 0 4 32 3 777.6 0 3 33 50 777.6 0 50 34 10 777.6 0 10 35 11 777.6 0 11 36 98 777.6 0 98 37 12 777.6 0 12 38 27 777.6 0 27 39 12 777.6 0 12 40 18 777.6 0 18 41 5 777.6 0 5 42 12 777.6 0 12 43 20 777.6 0 20 44 93 777.6 0 93 45 115 777.6 0 115 46 3 777.6 0 3 47 4 777.6 0 4 48 11 777.6 0 11 49 8 777.6 0 8 50 8 777.6 0 8 51 9 777.6 0 9 52 13 777.6 0 13 53 7 777.6 0 7 54 8 777.6 0 8 55 5 777.6 0 5 56 9 777.6 0 9 57 17 777.6 0 17 58 162 777.6 0 162 59 47 777.6 0 47 60 97 777.6 0 97 61 10 777.6 0 10 62 16 777.6 0 16 63 37 777.6 0 37 64 7 777.6 0 7 65 2 777.6 0 2 66 6 777.6 0 6 67 5 777.6 0 5 68 3 777.6 0 3 69 8 777.6 0 8 70 27 777.6 0 27 71 11 777.6 0 11 72 9 777.6 0 9 73 10 777.6 0 10 74 9 777.6 0 9 75 19 777.6 0 19 76 470 777.6 0 470 77 194 777.6 0 194 78 217 777.6 0 217 79 16 777.6 0 16 80 331 777.6 0 331 81 81 777.6 0 81 82 75 777.6 0 75 83 18 777.6 0 18 84 11 777.6 0 11 85 10 777.6 0 10 86 11 777.6 0 11 87 79 777.6 0 79 88 22 777.6 0 22 89 8 777.6 0 8 90 25 777.6 0 25 91 34 777.6 0 34 92 11 777.6 0 11 93 5 777.6 0 5 94 12 777.6 0 12 95 18 777.6 0 18 96 31 777.6 0 31 97 76 777.6 0 76 98 109 777.6 0 109 99 26 777.6 0 26 100 107 777.6 0 107 101 218 777.6 0 218 102 254 777.6 0 254 103 12 777.6 0 12 104 38 777.6 0 38 105 16 777.6 0 16 106 10 777.6 0 10 107 12 777.6 0 12 108 17 777.6 0 17 109 14 777.6 0 14 110 164 777.6 0 164 111 111 777.6 0 111 112 408 777.6 0 408 113 13 777.6 0 13 114 3 777.6 0 3 116 17 777.6 0 17 117 16 777.6 0 16 118 32 777.6 0 32 119 17 777.6 0 17 120 14 777.6 0 14 121 15 777.6 0 15 122 20 777.6 0 20 123 9 777.6 0 9 124 10 777.6 0 10 125 7 777.6 0 7 126 6 777.6 0 6 127 18 777.6 0 18 128 13 777.6 0 13 129 18 777.6 0 18 130 12 777.6 0 12 131 16 777.6 0 16 132 22 777.6 0 22 133 19 777.6 0 19 134 17 777.6 0 17 135 19 777.6 0 19 136 17 777.6 0 17 137 19 777.6 0 19 138 22 777.6 0 22 139 19 777.6 0 19 140 17 777.6 0 17 141 14 777.6 0 14 142 34 777.6 0 34 143 20 777.6 0 20 144 19 777.6 0 19 145 7 777.6 0 7 146 12 777.6 0 12 147 26 777.6 0 26 148 14 777.6 0 14 149 16 777.6 0 16 150 10 777.6 0 10 151 21 777.6 0 21 152 24 777.6 0 24 153 8 777.6 0 8 154 14 777.6 0 14 155 19 777.6 0 19 156 16 777.6 0 16 157 78 777.6 0 78 158 18 777.6 0 18 159 28 777.6 0 28 160 61 777.6 0 61 161 30 777.6 0 30 162 42 777.6 0 42 163 37 777.6 0 37 164 22 777.6 0 22 165 30 777.6 0 30 166 32 777.6 0 32 167 43 777.6 0 43 168 221 777.6 0 221 169 67 777.6 0 67 170 33 777.6 0 33 171 31 777.6 0 31 172 9 777.6 0 9 173 5 777.6 0 5 174 7 777.6 0 7 175 18 777.6 0 18 176 17 777.6 0 17 177 23 777.6 0 23 178 21 777.6 0 21 179 13 777.6 0 13 180 15 777.6 0 15 181 21 777.6 0 21 182 45 777.6 0 45 183 34 777.6 0 34 184 44 777.6 0 44 185 27 777.6 0 27 186 29 777.6 0 29 187 35 777.6 0 35 188 30 777.6 0 30 189 33 777.6 0 33 190 13 777.6 0 13 191 13 777.6 0 13 192 18 777.6 0 18 193 28 777.6 0 28 194 27 777.6 0 27 195 42 777.6 0 42 196 39 777.6 0 39 197 25 777.6 0 25 198 36 777.6 0 36 199 86 777.6 0 86 200 136 777.6 0 136 201 92 777.6 0 92 202 38 777.6 0 38 203 44 777.6 0 44 204 159 777.6 0 159 205 55 777.6 0 55 206 52 777.6 0 52 207 102 777.6 0 102 208 36 777.6 0 36 209 25 777.6 0 25 210 12 777.6 0 12 211 20 777.6 0 20 212 36 777.6 0 36 213 42 777.6 0 42 214 74 777.6 0 74 215 69 777.6 0 69 216 57 777.6 0 57 217 44 777.6 0 44 218 61 777.6 0 61 219 52 777.6 0 52 220 43 777.6 0 43 221 98 777.6 0 98 222 98 777.6 0 98 223 104 777.6 0 104 224 137 777.6 0 137 225 63 777.6 0 63 226 51 777.6 0 51 227 42 777.6 0 42 228 62 777.6 0 62 229 57 777.6 0 57 230 37 777.6 0 37 231 23 777.6 0 23 232 33 777.6 0 33 233 33 777.6 0 33 234 14 777.6 0 14 235 16 777.6 0 16 236 39 777.6 0 39 237 46 777.6 0 46 238 39 777.6 0 39 239 40 777.6 0 40 240 39 777.6 0 39 241 52 777.6 0 52 242 35 777.6 0 35 243 30 777.6 0 30 244 36 777.6 0 36 245 34 777.6 0 34 246 22 777.6 0 22 247 15 777.6 0 15 248 43 777.6 0 43 249 72 777.6 0 72 250 40 777.6 0 40 251 30 777.6 0 30 252 22 777.6 0 22 253 59 777.6 0 59 254 41 777.6 0 41 255 50 777.6 0 50 256 27 777.6 0 27 257 52 777.6 0 52 258 77 777.6 0 77 259 54 777.6 0 54 260 53 777.6 0 53 261 23 777.6 0 23 262 19 777.6 0 19 263 25 777.6 0 25 264 25 777.6 0 25 265 31 777.6 0 31 266 21 777.6 0 21 267 33 777.6 0 33 268 75 777.6 0 75 269 87 777.6 0 87 270 44 777.6 0 44 271 17 777.6 0 17 272 34 777.6 0 34 273 21 777.6 0 21 274 25 777.6 0 25 275 47 777.6 0 47 276 38 777.6 0 38 277 39 777.6 0 39 278 23 777.6 0 23 279 23 777.6 0 23 280 33 777.6 0 33 281 67 777.6 0 67 282 46 777.6 0 46 283 43 777.6 0 43 284 20 777.6 0 20 285 17 777.6 0 17 286 51 777.6 0 51 287 48 777.6 0 48 288 49 777.6 0 49 289 155 777.6 0 155 290 62 777.6 0 62 291 70 777.6 0 70 292 33 777.6 0 33 293 37 777.6 0 37 294 114 777.6 0 114 295 106 777.6 0 106 296 108 777.6 0 108 297 30 777.6 0 30 298 48 777.6 0 48 299 45 777.6 0 45 300 25 777.6 0 25 301 17 777.6 0 17 Finished in 3.57 s (55 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 64,478 Reads with adapters: 42,835 (66.4%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 42,821 (66.4%) Total basepairs processed: 18,145,828 bp Total written (filtered): 11,642,637 bp (64.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 42835 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 7 0.0 3 0 0 5 2 19 153 0.0 3 0 134 8 6 5 20 41805 0.0 4 40433 1148 151 47 26 21 732 0.0 4 608 111 4 9 22 12 0.0 4 0 2 6 1 3 23 40 0.0 4 7 6 1 20 6 24 2 0.0 4 0 0 0 1 1 25 3 0.0 4 2 0 1 31 1 0.0 4 1 93 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 31 0.0 4 0 0 0 0 31 128 27 0.0 4 0 0 0 0 27 131 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 177 2 0.0 4 0 0 0 0 2 179 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 212 2 0.0 4 0 0 0 0 2 219 1 0.0 4 0 0 0 1 221 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 1 283 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.85 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 42,821 Reads with adapters: 3,570 (8.3%) Reads written (passing filters): 42,821 (100.0%) Total basepairs processed: 11,642,637 bp Total written (filtered): 11,592,198 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3570 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 3.4% G: 91.1% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 5 167.3 0 5 5 1 41.8 0 1 6 2 10.5 0 2 9 950 0.2 0 47 903 10 681 0.0 1 19 662 11 1860 0.0 1 2 1858 12 7 0.0 1 0 7 13 6 0.0 1 0 6 150 3 0.0 1 1 2 158 1 0.0 1 0 1 177 1 0.0 1 1 208 8 0.0 1 5 3 266 44 0.0 1 42 2 267 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.