This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 105.84 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 421,199 (13.2%) Reads that were too short: 19,088 (0.6%) Reads written (passing filters): 402,111 (12.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 69,539,199 bp (7.3%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 421199 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 402 777.6 0 402 7 32236 777.6 0 32236 8 9808 777.6 0 9808 9 10 777.6 0 10 10 10 777.6 0 10 11 94 777.6 0 94 12 28 777.6 0 28 13 14 777.6 0 14 14 16 777.6 0 16 15 4 777.6 0 4 16 8 777.6 0 8 17 14 777.6 0 14 18 9 777.6 0 9 19 9 777.6 0 9 20 8 777.6 0 8 21 7 777.6 0 7 22 10 777.6 0 10 23 7 777.6 0 7 24 13 777.6 0 13 25 16 777.6 0 16 26 13 777.6 0 13 27 17 777.6 0 17 28 11 777.6 0 11 29 4 777.6 0 4 30 2 777.6 0 2 31 15 777.6 0 15 32 19 777.6 0 19 33 9 777.6 0 9 34 22 777.6 0 22 35 6 777.6 0 6 36 12 777.6 0 12 37 12 777.6 0 12 38 93 777.6 0 93 39 64 777.6 0 64 40 15 777.6 0 15 41 8 777.6 0 8 42 17 777.6 0 17 43 50 777.6 0 50 44 18 777.6 0 18 45 9 777.6 0 9 46 15 777.6 0 15 47 14 777.6 0 14 48 6 777.6 0 6 49 5 777.6 0 5 50 10 777.6 0 10 51 10 777.6 0 10 52 6 777.6 0 6 53 10 777.6 0 10 54 11 777.6 0 11 55 10 777.6 0 10 56 9 777.6 0 9 57 13 777.6 0 13 58 9 777.6 0 9 59 15 777.6 0 15 60 8 777.6 0 8 61 6 777.6 0 6 62 9 777.6 0 9 63 9 777.6 0 9 64 90 777.6 0 90 65 75 777.6 0 75 66 96 777.6 0 96 67 9 777.6 0 9 68 11 777.6 0 11 69 48 777.6 0 48 70 18 777.6 0 18 71 35 777.6 0 35 72 30 777.6 0 30 73 22 777.6 0 22 74 18 777.6 0 18 75 28 777.6 0 28 76 29 777.6 0 29 77 63 777.6 0 63 78 117 777.6 0 117 79 54 777.6 0 54 80 142 777.6 0 142 81 134 777.6 0 134 82 141 777.6 0 141 83 175 777.6 0 175 84 142 777.6 0 142 85 247 777.6 0 247 86 57 777.6 0 57 87 43 777.6 0 43 88 146 777.6 0 146 89 293 777.6 0 293 90 297 777.6 0 297 91 231 777.6 0 231 92 67 777.6 0 67 93 657 777.6 0 657 94 91 777.6 0 91 95 223 777.6 0 223 96 141 777.6 0 141 97 70 777.6 0 70 98 116 777.6 0 116 99 218 777.6 0 218 100 224 777.6 0 224 101 679 777.6 0 679 102 831 777.6 0 831 103 147 777.6 0 147 104 183 777.6 0 183 105 228 777.6 0 228 106 180 777.6 0 180 107 148 777.6 0 148 108 51 777.6 0 51 109 82 777.6 0 82 110 278 777.6 0 278 111 668 777.6 0 668 112 341 777.6 0 341 113 231 777.6 0 231 114 259 777.6 0 259 115 600 777.6 0 600 116 147 777.6 0 147 117 79 777.6 0 79 118 137 777.6 0 137 119 150 777.6 0 150 120 92 777.6 0 92 121 116 777.6 0 116 122 305 777.6 0 305 123 211 777.6 0 211 124 431 777.6 0 431 125 731 777.6 0 731 126 192 777.6 0 192 127 381 777.6 0 381 128 231 777.6 0 231 129 257 777.6 0 257 130 356 777.6 0 356 131 353 777.6 0 353 132 434 777.6 0 434 133 183 777.6 0 183 134 198 777.6 0 198 135 378 777.6 0 378 136 446 777.6 0 446 137 776 777.6 0 776 138 1363 777.6 0 1363 139 1846 777.6 0 1846 140 3939 777.6 0 3939 141 4535 777.6 0 4535 142 123289 777.6 0 123289 143 147864 777.6 0 147864 144 39158 777.6 0 39158 145 399 777.6 0 399 146 227 777.6 0 227 147 371 777.6 0 371 148 247 777.6 0 247 149 317 777.6 0 317 150 131 777.6 0 131 151 106 777.6 0 106 152 131 777.6 0 131 153 116 777.6 0 116 154 94 777.6 0 94 155 403 777.6 0 403 156 192 777.6 0 192 157 367 777.6 0 367 158 316 777.6 0 316 159 177 777.6 0 177 160 251 777.6 0 251 161 325 777.6 0 325 162 6101 777.6 0 6101 163 1351 777.6 0 1351 164 2776 777.6 0 2776 165 126 777.6 0 126 166 126 777.6 0 126 167 308 777.6 0 308 168 243 777.6 0 243 169 359 777.6 0 359 170 104 777.6 0 104 171 85 777.6 0 85 172 44 777.6 0 44 173 90 777.6 0 90 174 97 777.6 0 97 175 179 777.6 0 179 176 118 777.6 0 118 177 182 777.6 0 182 178 223 777.6 0 223 179 180 777.6 0 180 180 172 777.6 0 172 181 94 777.6 0 94 182 110 777.6 0 110 183 137 777.6 0 137 184 259 777.6 0 259 185 252 777.6 0 252 186 163 777.6 0 163 187 145 777.6 0 145 188 201 777.6 0 201 189 300 777.6 0 300 190 471 777.6 0 471 191 185 777.6 0 185 192 109 777.6 0 109 193 177 777.6 0 177 194 112 777.6 0 112 195 84 777.6 0 84 196 87 777.6 0 87 197 140 777.6 0 140 198 356 777.6 0 356 199 207 777.6 0 207 200 55 777.6 0 55 201 37 777.6 0 37 202 57 777.6 0 57 203 226 777.6 0 226 204 470 777.6 0 470 205 330 777.6 0 330 206 198 777.6 0 198 207 144 777.6 0 144 208 96 777.6 0 96 209 191 777.6 0 191 210 258 777.6 0 258 211 188 777.6 0 188 212 189 777.6 0 189 213 165 777.6 0 165 214 318 777.6 0 318 215 353 777.6 0 353 216 251 777.6 0 251 217 279 777.6 0 279 218 252 777.6 0 252 219 167 777.6 0 167 220 68 777.6 0 68 221 42 777.6 0 42 222 87 777.6 0 87 223 305 777.6 0 305 224 600 777.6 0 600 225 170 777.6 0 170 226 162 777.6 0 162 227 244 777.6 0 244 228 135 777.6 0 135 229 159 777.6 0 159 230 161 777.6 0 161 231 80 777.6 0 80 232 73 777.6 0 73 233 122 777.6 0 122 234 353 777.6 0 353 235 265 777.6 0 265 236 166 777.6 0 166 237 159 777.6 0 159 238 96 777.6 0 96 239 104 777.6 0 104 240 82 777.6 0 82 241 59 777.6 0 59 242 76 777.6 0 76 243 157 777.6 0 157 244 280 777.6 0 280 245 344 777.6 0 344 246 211 777.6 0 211 247 131 777.6 0 131 248 83 777.6 0 83 249 57 777.6 0 57 250 69 777.6 0 69 251 80 777.6 0 80 252 119 777.6 0 119 253 266 777.6 0 266 254 234 777.6 0 234 255 186 777.6 0 186 256 150 777.6 0 150 257 143 777.6 0 143 258 276 777.6 0 276 259 110 777.6 0 110 260 109 777.6 0 109 261 145 777.6 0 145 262 135 777.6 0 135 263 171 777.6 0 171 264 189 777.6 0 189 265 236 777.6 0 236 266 405 777.6 0 405 267 205 777.6 0 205 268 734 777.6 0 734 269 218 777.6 0 218 270 126 777.6 0 126 271 91 777.6 0 91 272 136 777.6 0 136 273 157 777.6 0 157 274 136 777.6 0 136 275 95 777.6 0 95 276 100 777.6 0 100 277 87 777.6 0 87 278 133 777.6 0 133 279 330 777.6 0 330 280 184 777.6 0 184 281 124 777.6 0 124 282 92 777.6 0 92 283 100 777.6 0 100 284 173 777.6 0 173 285 167 777.6 0 167 286 183 777.6 0 183 287 113 777.6 0 113 288 118 777.6 0 118 289 206 777.6 0 206 290 139 777.6 0 139 291 151 777.6 0 151 292 461 777.6 0 461 293 265 777.6 0 265 294 227 777.6 0 227 295 238 777.6 0 238 296 153 777.6 0 153 297 142 777.6 0 142 298 294 777.6 0 294 299 174 777.6 0 174 300 354 777.6 0 354 301 296 777.6 0 296 Finished in 18.07 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 402,111 Reads with adapters: 10,209 (2.5%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 10,181 (2.5%) Total basepairs processed: 69,539,199 bp Total written (filtered): 2,755,804 bp (4.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10209 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 29 0.0 3 0 25 1 1 2 20 9908 0.0 4 9605 267 25 9 2 21 16 0.0 4 2 14 23 5 0.0 4 0 0 0 4 1 24 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 1 35 2 0.0 4 0 0 0 0 2 37 7 0.0 4 0 0 0 0 7 38 2 0.0 4 0 0 0 0 2 44 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 68 3 0.0 4 0 0 0 0 3 70 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 203 0.0 4 0 0 0 0 203 133 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 291 2 0.0 4 0 0 0 0 2 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.46 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 10,181 Reads with adapters: 1,633 (16.0%) Reads written (passing filters): 10,181 (100.0%) Total basepairs processed: 2,755,804 bp Total written (filtered): 2,737,268 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1633 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 1.8% G: 93.8% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 39.8 0 1 9 1 0.0 0 0 1 10 23 0.0 1 1 22 11 1598 0.0 1 3 1595 12 5 0.0 1 1 4 15 1 0.0 1 0 1 136 1 0.0 1 0 1 153 1 0.0 1 1 159 1 0.0 1 0 1 192 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.