This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 103.62 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 99,964 (3.1%) Reads that were too short: 34,960 (1.1%) Reads written (passing filters): 65,004 (2.0%) Total basepairs processed: 958,672,960 bp Total written (filtered): 13,709,151 bp (1.4%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 99964 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3681 777.6 0 3681 7 4035 777.6 0 4035 8 152 777.6 0 152 9 17398 777.6 0 17398 10 17 777.6 0 17 11 11 777.6 0 11 12 5 777.6 0 5 13 5 777.6 0 5 14 1 777.6 0 1 15 6 777.6 0 6 16 10 777.6 0 10 17 9 777.6 0 9 18 8 777.6 0 8 19 9 777.6 0 9 20 5 777.6 0 5 21 12 777.6 0 12 22 9 777.6 0 9 23 12 777.6 0 12 24 8 777.6 0 8 25 6 777.6 0 6 26 4 777.6 0 4 27 7 777.6 0 7 28 8 777.6 0 8 29 14 777.6 0 14 30 19 777.6 0 19 31 13 777.6 0 13 32 15 777.6 0 15 33 8 777.6 0 8 34 6 777.6 0 6 35 7 777.6 0 7 36 11 777.6 0 11 37 15 777.6 0 15 38 10 777.6 0 10 39 9 777.6 0 9 40 30 777.6 0 30 41 14 777.6 0 14 42 18 777.6 0 18 43 7 777.6 0 7 44 7 777.6 0 7 45 6 777.6 0 6 46 6 777.6 0 6 47 9 777.6 0 9 48 7 777.6 0 7 49 11 777.6 0 11 50 3 777.6 0 3 51 14 777.6 0 14 52 11 777.6 0 11 53 16 777.6 0 16 54 14 777.6 0 14 55 38 777.6 0 38 56 392 777.6 0 392 57 9 777.6 0 9 58 10 777.6 0 10 59 5 777.6 0 5 60 12 777.6 0 12 61 10 777.6 0 10 62 4 777.6 0 4 63 8 777.6 0 8 64 5 777.6 0 5 65 8 777.6 0 8 66 7 777.6 0 7 67 7 777.6 0 7 68 8 777.6 0 8 69 12 777.6 0 12 70 12 777.6 0 12 71 25 777.6 0 25 72 30 777.6 0 30 73 224 777.6 0 224 74 230 777.6 0 230 75 176 777.6 0 176 76 49 777.6 0 49 77 20 777.6 0 20 78 53 777.6 0 53 79 61 777.6 0 61 80 308 777.6 0 308 81 97 777.6 0 97 82 214 777.6 0 214 83 125 777.6 0 125 84 272 777.6 0 272 85 64 777.6 0 64 86 54 777.6 0 54 87 84 777.6 0 84 88 49 777.6 0 49 89 46 777.6 0 46 90 162 777.6 0 162 91 162 777.6 0 162 92 330 777.6 0 330 93 95 777.6 0 95 94 27 777.6 0 27 95 53 777.6 0 53 96 41 777.6 0 41 97 122 777.6 0 122 98 208 777.6 0 208 99 200 777.6 0 200 100 296 777.6 0 296 101 895 777.6 0 895 102 577 777.6 0 577 103 857 777.6 0 857 104 463 777.6 0 463 105 66 777.6 0 66 106 98 777.6 0 98 107 52 777.6 0 52 108 40 777.6 0 40 109 132 777.6 0 132 110 115 777.6 0 115 111 205 777.6 0 205 112 176 777.6 0 176 113 345 777.6 0 345 114 88 777.6 0 88 115 276 777.6 0 276 116 426 777.6 0 426 117 421 777.6 0 421 118 289 777.6 0 289 119 189 777.6 0 189 120 161 777.6 0 161 121 136 777.6 0 136 122 129 777.6 0 129 123 461 777.6 0 461 124 399 777.6 0 399 125 348 777.6 0 348 126 202 777.6 0 202 127 79 777.6 0 79 128 63 777.6 0 63 129 82 777.6 0 82 130 556 777.6 0 556 131 570 777.6 0 570 132 757 777.6 0 757 133 518 777.6 0 518 134 246 777.6 0 246 135 187 777.6 0 187 136 329 777.6 0 329 137 476 777.6 0 476 138 463 777.6 0 463 139 311 777.6 0 311 140 117 777.6 0 117 141 238 777.6 0 238 142 590 777.6 0 590 143 642 777.6 0 642 144 749 777.6 0 749 145 812 777.6 0 812 146 249 777.6 0 249 147 123 777.6 0 123 148 258 777.6 0 258 149 556 777.6 0 556 150 335 777.6 0 335 151 451 777.6 0 451 152 290 777.6 0 290 153 255 777.6 0 255 154 429 777.6 0 429 155 350 777.6 0 350 156 577 777.6 0 577 157 845 777.6 0 845 158 815 777.6 0 815 159 269 777.6 0 269 160 287 777.6 0 287 161 181 777.6 0 181 162 226 777.6 0 226 163 266 777.6 0 266 164 351 777.6 0 351 165 203 777.6 0 203 166 228 777.6 0 228 167 193 777.6 0 193 168 288 777.6 0 288 169 493 777.6 0 493 170 985 777.6 0 985 171 1080 777.6 0 1080 172 402 777.6 0 402 173 134 777.6 0 134 174 71 777.6 0 71 175 106 777.6 0 106 176 223 777.6 0 223 177 831 777.6 0 831 178 818 777.6 0 818 179 682 777.6 0 682 180 404 777.6 0 404 181 117 777.6 0 117 182 217 777.6 0 217 183 198 777.6 0 198 184 231 777.6 0 231 185 200 777.6 0 200 186 141 777.6 0 141 187 269 777.6 0 269 188 697 777.6 0 697 189 583 777.6 0 583 190 226 777.6 0 226 191 114 777.6 0 114 192 96 777.6 0 96 193 99 777.6 0 99 194 83 777.6 0 83 195 363 777.6 0 363 196 255 777.6 0 255 197 320 777.6 0 320 198 226 777.6 0 226 199 200 777.6 0 200 200 334 777.6 0 334 201 308 777.6 0 308 202 229 777.6 0 229 203 246 777.6 0 246 204 246 777.6 0 246 205 307 777.6 0 307 206 250 777.6 0 250 207 327 777.6 0 327 208 254 777.6 0 254 209 399 777.6 0 399 210 385 777.6 0 385 211 443 777.6 0 443 212 308 777.6 0 308 213 232 777.6 0 232 214 373 777.6 0 373 215 396 777.6 0 396 216 397 777.6 0 397 217 335 777.6 0 335 218 195 777.6 0 195 219 122 777.6 0 122 220 143 777.6 0 143 221 364 777.6 0 364 222 384 777.6 0 384 223 491 777.6 0 491 224 344 777.6 0 344 225 382 777.6 0 382 226 346 777.6 0 346 227 246 777.6 0 246 228 405 777.6 0 405 229 224 777.6 0 224 230 139 777.6 0 139 231 122 777.6 0 122 232 194 777.6 0 194 233 197 777.6 0 197 234 166 777.6 0 166 235 148 777.6 0 148 236 143 777.6 0 143 237 222 777.6 0 222 238 206 777.6 0 206 239 236 777.6 0 236 240 203 777.6 0 203 241 269 777.6 0 269 242 527 777.6 0 527 243 542 777.6 0 542 244 398 777.6 0 398 245 323 777.6 0 323 246 235 777.6 0 235 247 257 777.6 0 257 248 483 777.6 0 483 249 564 777.6 0 564 250 460 777.6 0 460 251 502 777.6 0 502 252 658 777.6 0 658 253 436 777.6 0 436 254 214 777.6 0 214 255 145 777.6 0 145 256 119 777.6 0 119 257 137 777.6 0 137 258 200 777.6 0 200 259 225 777.6 0 225 260 247 777.6 0 247 261 304 777.6 0 304 262 328 777.6 0 328 263 507 777.6 0 507 264 676 777.6 0 676 265 447 777.6 0 447 266 174 777.6 0 174 267 130 777.6 0 130 268 155 777.6 0 155 269 244 777.6 0 244 270 462 777.6 0 462 271 486 777.6 0 486 272 542 777.6 0 542 273 425 777.6 0 425 274 331 777.6 0 331 275 199 777.6 0 199 276 372 777.6 0 372 277 555 777.6 0 555 278 298 777.6 0 298 279 156 777.6 0 156 280 168 777.6 0 168 281 315 777.6 0 315 282 492 777.6 0 492 283 815 777.6 0 815 284 1891 777.6 0 1891 285 1026 777.6 0 1026 286 707 777.6 0 707 287 211 777.6 0 211 288 303 777.6 0 303 289 272 777.6 0 272 290 511 777.6 0 511 291 491 777.6 0 491 292 392 777.6 0 392 293 243 777.6 0 243 294 197 777.6 0 197 295 202 777.6 0 202 296 364 777.6 0 364 297 354 777.6 0 354 298 200 777.6 0 200 299 288 777.6 0 288 300 653 777.6 0 653 301 384 777.6 0 384 Finished in 3.31 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 65,004 Reads with adapters: 7,580 (11.7%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 7,570 (11.6%) Total basepairs processed: 13,709,151 bp Total written (filtered): 2,057,011 bp (15.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7580 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 0 1 19 10 0.0 3 0 9 1 20 3892 0.0 4 3820 56 9 4 3 21 9 0.0 4 1 8 22 14 0.0 4 2 10 1 1 23 3487 0.0 4 3384 82 16 3 2 24 5 0.0 4 2 3 25 1 0.0 4 1 34 1 0.0 4 0 0 0 1 38 1 0.0 4 0 0 0 0 1 40 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 0 1 44 77 0.0 4 1 0 0 0 76 47 1 0.0 4 0 1 48 1 0.0 4 0 0 0 0 1 77 1 0.0 4 1 78 1 0.0 4 1 89 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 61 0.0 4 0 0 0 0 61 141 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 7,570 Reads with adapters: 358 (4.7%) Reads written (passing filters): 7,570 (100.0%) Total basepairs processed: 2,057,011 bp Total written (filtered): 2,024,825 bp (98.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 358 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 33.0% C: 4.2% G: 60.9% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 118.3 0 3 4 1 29.6 0 1 9 33 0.0 0 2 31 10 56 0.0 1 3 53 11 139 0.0 1 0 139 12 9 0.0 1 0 9 15 1 0.0 1 0 1 150 1 0.0 1 1 152 4 0.0 1 2 2 244 1 0.0 1 1 257 9 0.0 1 8 1 260 22 0.0 1 21 1 261 77 0.0 1 72 5 262 1 0.0 1 1 270 1 0.0 1 1