This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.25 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 95,541 (3.0%) Reads that were too short: 64,221 (2.0%) Reads written (passing filters): 31,320 (1.0%) Total basepairs processed: 958,672,960 bp Total written (filtered): 6,823,696 bp (0.7%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 95541 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 93 777.6 0 93 7 198 777.6 0 198 8 7201 777.6 0 7201 9 4216 777.6 0 4216 10 5 777.6 0 5 11 3 777.6 0 3 12 33 777.6 0 33 13 5 777.6 0 5 14 6 777.6 0 6 15 4 777.6 0 4 16 3 777.6 0 3 17 8 777.6 0 8 18 4 777.6 0 4 19 3 777.6 0 3 20 8 777.6 0 8 21 6 777.6 0 6 22 4 777.6 0 4 23 3 777.6 0 3 24 1 777.6 0 1 25 2 777.6 0 2 26 6 777.6 0 6 27 4 777.6 0 4 28 10 777.6 0 10 29 6 777.6 0 6 30 5 777.6 0 5 31 14 777.6 0 14 32 5 777.6 0 5 33 12 777.6 0 12 34 11 777.6 0 11 35 7 777.6 0 7 36 12 777.6 0 12 37 50 777.6 0 50 38 14 777.6 0 14 39 19 777.6 0 19 40 15 777.6 0 15 41 4 777.6 0 4 42 7 777.6 0 7 43 9 777.6 0 9 44 7 777.6 0 7 45 7 777.6 0 7 46 8 777.6 0 8 47 11 777.6 0 11 48 8 777.6 0 8 49 9 777.6 0 9 50 14 777.6 0 14 51 41 777.6 0 41 52 35 777.6 0 35 53 4 777.6 0 4 54 16 777.6 0 16 55 9 777.6 0 9 56 200 777.6 0 200 57 124 777.6 0 124 58 80 777.6 0 80 59 97 777.6 0 97 60 3 777.6 0 3 61 17 777.6 0 17 62 19 777.6 0 19 63 16 777.6 0 16 64 37 777.6 0 37 65 14 777.6 0 14 66 12 777.6 0 12 67 43 777.6 0 43 68 12 777.6 0 12 69 55 777.6 0 55 70 103 777.6 0 103 71 53 777.6 0 53 72 13 777.6 0 13 73 16 777.6 0 16 74 16 777.6 0 16 75 40 777.6 0 40 76 57 777.6 0 57 77 28 777.6 0 28 78 32 777.6 0 32 79 58 777.6 0 58 80 56 777.6 0 56 81 36 777.6 0 36 82 55 777.6 0 55 83 65 777.6 0 65 84 56 777.6 0 56 85 66 777.6 0 66 86 72 777.6 0 72 87 69 777.6 0 69 88 139 777.6 0 139 89 1548 777.6 0 1548 90 1287 777.6 0 1287 91 1888 777.6 0 1888 92 24 777.6 0 24 93 28 777.6 0 28 94 50 777.6 0 50 95 58 777.6 0 58 96 127 777.6 0 127 97 42 777.6 0 42 98 67 777.6 0 67 99 104 777.6 0 104 100 367 777.6 0 367 101 80 777.6 0 80 102 178 777.6 0 178 103 17 777.6 0 17 104 13 777.6 0 13 105 28 777.6 0 28 106 17 777.6 0 17 107 12 777.6 0 12 108 26 777.6 0 26 109 15 777.6 0 15 110 72 777.6 0 72 111 16 777.6 0 16 112 75 777.6 0 75 113 17 777.6 0 17 114 33 777.6 0 33 115 57 777.6 0 57 116 44 777.6 0 44 117 46 777.6 0 46 118 19 777.6 0 19 119 23 777.6 0 23 120 59 777.6 0 59 121 29 777.6 0 29 122 48 777.6 0 48 123 13 777.6 0 13 124 11 777.6 0 11 125 31 777.6 0 31 126 24 777.6 0 24 127 27 777.6 0 27 128 28 777.6 0 28 129 143 777.6 0 143 130 26 777.6 0 26 131 38 777.6 0 38 132 617 777.6 0 617 133 248 777.6 0 248 134 570 777.6 0 570 135 61 777.6 0 61 136 18 777.6 0 18 137 41 777.6 0 41 138 29 777.6 0 29 139 38 777.6 0 38 140 69 777.6 0 69 141 28 777.6 0 28 142 22 777.6 0 22 143 23 777.6 0 23 144 24 777.6 0 24 145 29 777.6 0 29 146 30 777.6 0 30 147 82 777.6 0 82 148 796 777.6 0 796 149 1044 777.6 0 1044 150 494 777.6 0 494 151 18 777.6 0 18 152 12 777.6 0 12 153 20 777.6 0 20 154 16 777.6 0 16 155 33 777.6 0 33 156 31 777.6 0 31 157 73 777.6 0 73 158 38 777.6 0 38 159 30 777.6 0 30 160 54 777.6 0 54 161 20 777.6 0 20 162 32 777.6 0 32 163 19 777.6 0 19 164 36 777.6 0 36 165 80 777.6 0 80 166 87 777.6 0 87 167 92 777.6 0 92 168 599 777.6 0 599 169 188 777.6 0 188 170 98 777.6 0 98 171 20 777.6 0 20 172 25 777.6 0 25 173 50 777.6 0 50 174 61 777.6 0 61 175 117 777.6 0 117 176 2127 777.6 0 2127 177 364 777.6 0 364 178 1068 777.6 0 1068 179 33 777.6 0 33 180 36 777.6 0 36 181 31 777.6 0 31 182 22 777.6 0 22 183 30 777.6 0 30 184 67 777.6 0 67 185 69 777.6 0 69 186 68 777.6 0 68 187 20 777.6 0 20 188 9 777.6 0 9 189 11 777.6 0 11 190 15 777.6 0 15 191 20 777.6 0 20 192 28 777.6 0 28 193 94 777.6 0 94 194 64 777.6 0 64 195 29 777.6 0 29 196 33 777.6 0 33 197 24 777.6 0 24 198 45 777.6 0 45 199 49 777.6 0 49 200 34 777.6 0 34 201 19 777.6 0 19 202 35 777.6 0 35 203 59 777.6 0 59 204 53 777.6 0 53 205 86 777.6 0 86 206 68 777.6 0 68 207 248 777.6 0 248 208 292 777.6 0 292 209 103 777.6 0 103 210 94 777.6 0 94 211 49 777.6 0 49 212 55 777.6 0 55 213 70 777.6 0 70 214 68 777.6 0 68 215 99 777.6 0 99 216 154 777.6 0 154 217 131 777.6 0 131 218 198 777.6 0 198 219 330 777.6 0 330 220 5011 777.6 0 5011 221 2528 777.6 0 2528 222 2748 777.6 0 2748 223 28 777.6 0 28 224 53 777.6 0 53 225 107 777.6 0 107 226 201 777.6 0 201 227 97 777.6 0 97 228 58 777.6 0 58 229 56 777.6 0 56 230 61 777.6 0 61 231 50 777.6 0 50 232 37 777.6 0 37 233 30 777.6 0 30 234 27 777.6 0 27 235 48 777.6 0 48 236 47 777.6 0 47 237 55 777.6 0 55 238 25 777.6 0 25 239 101 777.6 0 101 240 88 777.6 0 88 241 162 777.6 0 162 242 38 777.6 0 38 243 32 777.6 0 32 244 85 777.6 0 85 245 94 777.6 0 94 246 103 777.6 0 103 247 48 777.6 0 48 248 41 777.6 0 41 249 71 777.6 0 71 250 37 777.6 0 37 251 38 777.6 0 38 252 28 777.6 0 28 253 86 777.6 0 86 254 93 777.6 0 93 255 260 777.6 0 260 256 374 777.6 0 374 257 187 777.6 0 187 258 73 777.6 0 73 259 69 777.6 0 69 260 60 777.6 0 60 261 69 777.6 0 69 262 54 777.6 0 54 263 63 777.6 0 63 264 70 777.6 0 70 265 115 777.6 0 115 266 253 777.6 0 253 267 129 777.6 0 129 268 883 777.6 0 883 269 1064 777.6 0 1064 270 870 777.6 0 870 271 423 777.6 0 423 272 216 777.6 0 216 273 157 777.6 0 157 274 860 777.6 0 860 275 523 777.6 0 523 276 368 777.6 0 368 277 59 777.6 0 59 278 115 777.6 0 115 279 265 777.6 0 265 280 490 777.6 0 490 281 1272 777.6 0 1272 282 1164 777.6 0 1164 283 18885 777.6 0 18885 284 5839 777.6 0 5839 285 9696 777.6 0 9696 286 1177 777.6 0 1177 287 52 777.6 0 52 288 146 777.6 0 146 289 662 777.6 0 662 290 1510 777.6 0 1510 291 145 777.6 0 145 292 46 777.6 0 46 293 168 777.6 0 168 294 119 777.6 0 119 295 78 777.6 0 78 296 209 777.6 0 209 297 38 777.6 0 38 298 106 777.6 0 106 299 128 777.6 0 128 300 64 777.6 0 64 301 44 777.6 0 44 Finished in 1.68 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 31,320 Reads with adapters: 4,268 (13.6%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 4,254 (13.6%) Total basepairs processed: 6,823,696 bp Total written (filtered): 1,150,589 bp (16.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4268 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 19 14 0.0 3 0 13 1 20 4167 0.0 4 4074 73 15 4 1 21 8 0.0 4 1 6 0 0 1 23 8 0.0 4 0 0 1 6 1 41 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 71 2 0.0 4 0 0 0 0 2 77 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 20 0.0 4 0 0 0 0 20 131 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 27 0.0 4 0 0 0 0 27 149 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 4,254 Reads with adapters: 3,420 (80.4%) Reads written (passing filters): 4,254 (100.0%) Total basepairs processed: 1,150,589 bp Total written (filtered): 242,994 bp (21.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3420 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.1% G: 0.6% T: 99.2% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "T" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 8 0.0 0 0 8 10 9 0.0 1 0 9 11 5 0.0 1 0 5 264 1 0.0 1 1 265 1 0.0 1 1 266 7 0.0 1 6 1 267 3308 0.0 1 3142 166 268 47 0.0 1 45 2 269 25 0.0 1 24 1 270 9 0.0 1 9 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.