This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 105.37 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 252,654 (7.9%) Reads that were too short: 115,059 (3.6%) Reads written (passing filters): 137,595 (4.3%) Total basepairs processed: 958,672,960 bp Total written (filtered): 22,098,682 bp (2.3%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 252654 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 54 777.6 0 54 7 1799 777.6 0 1799 8 13 777.6 0 13 9 14 777.6 0 14 10 17 777.6 0 17 11 15 777.6 0 15 12 13 777.6 0 13 13 13 777.6 0 13 14 20 777.6 0 20 15 10 777.6 0 10 16 13 777.6 0 13 17 15 777.6 0 15 18 10 777.6 0 10 19 13 777.6 0 13 20 8 777.6 0 8 21 14 777.6 0 14 22 14 777.6 0 14 23 13 777.6 0 13 24 15 777.6 0 15 25 27 777.6 0 27 26 36 777.6 0 36 27 10 777.6 0 10 28 14 777.6 0 14 29 9 777.6 0 9 30 8 777.6 0 8 31 11 777.6 0 11 32 11 777.6 0 11 33 12 777.6 0 12 34 15 777.6 0 15 35 11 777.6 0 11 36 14 777.6 0 14 37 7 777.6 0 7 38 13 777.6 0 13 39 7 777.6 0 7 40 8 777.6 0 8 41 10 777.6 0 10 42 13 777.6 0 13 43 21 777.6 0 21 44 15 777.6 0 15 45 18 777.6 0 18 46 17 777.6 0 17 47 16 777.6 0 16 48 12 777.6 0 12 49 11 777.6 0 11 50 14 777.6 0 14 51 27 777.6 0 27 52 10 777.6 0 10 53 7 777.6 0 7 54 9 777.6 0 9 55 9 777.6 0 9 56 53 777.6 0 53 57 175 777.6 0 175 58 19 777.6 0 19 59 15 777.6 0 15 60 59 777.6 0 59 61 13 777.6 0 13 62 17 777.6 0 17 63 12 777.6 0 12 64 8 777.6 0 8 65 5 777.6 0 5 66 26 777.6 0 26 67 29 777.6 0 29 68 36 777.6 0 36 69 13 777.6 0 13 70 15 777.6 0 15 71 16 777.6 0 16 72 161 777.6 0 161 73 132 777.6 0 132 74 386 777.6 0 386 75 289 777.6 0 289 76 487 777.6 0 487 77 523 777.6 0 523 78 1177 777.6 0 1177 79 197 777.6 0 197 80 269 777.6 0 269 81 400 777.6 0 400 82 360 777.6 0 360 83 401 777.6 0 401 84 314 777.6 0 314 85 424 777.6 0 424 86 165 777.6 0 165 87 702 777.6 0 702 88 613 777.6 0 613 89 202 777.6 0 202 90 756 777.6 0 756 91 681 777.6 0 681 92 89 777.6 0 89 93 1256 777.6 0 1256 94 1102 777.6 0 1102 95 1294 777.6 0 1294 96 462 777.6 0 462 97 165 777.6 0 165 98 415 777.6 0 415 99 242 777.6 0 242 100 170 777.6 0 170 101 392 777.6 0 392 102 427 777.6 0 427 103 236 777.6 0 236 104 718 777.6 0 718 105 711 777.6 0 711 106 340 777.6 0 340 107 3064 777.6 0 3064 108 718 777.6 0 718 109 395 777.6 0 395 110 306 777.6 0 306 111 517 777.6 0 517 112 388 777.6 0 388 113 569 777.6 0 569 114 560 777.6 0 560 115 223 777.6 0 223 116 399 777.6 0 399 117 224 777.6 0 224 118 305 777.6 0 305 119 550 777.6 0 550 120 888 777.6 0 888 121 934 777.6 0 934 122 818 777.6 0 818 123 737 777.6 0 737 124 531 777.6 0 531 125 677 777.6 0 677 126 907 777.6 0 907 127 991 777.6 0 991 128 1011 777.6 0 1011 129 1005 777.6 0 1005 130 497 777.6 0 497 131 541 777.6 0 541 132 566 777.6 0 566 133 2317 777.6 0 2317 134 9239 777.6 0 9239 135 7496 777.6 0 7496 136 9052 777.6 0 9052 137 1024 777.6 0 1024 138 505 777.6 0 505 139 680 777.6 0 680 140 1041 777.6 0 1041 141 724 777.6 0 724 142 748 777.6 0 748 143 342 777.6 0 342 144 437 777.6 0 437 145 1098 777.6 0 1098 146 1013 777.6 0 1013 147 1333 777.6 0 1333 148 2218 777.6 0 2218 149 735 777.6 0 735 150 645 777.6 0 645 151 481 777.6 0 481 152 1093 777.6 0 1093 153 751 777.6 0 751 154 1476 777.6 0 1476 155 1788 777.6 0 1788 156 2415 777.6 0 2415 157 4659 777.6 0 4659 158 12458 777.6 0 12458 159 2534 777.6 0 2534 160 1002 777.6 0 1002 161 814 777.6 0 814 162 721 777.6 0 721 163 536 777.6 0 536 164 624 777.6 0 624 165 805 777.6 0 805 166 1015 777.6 0 1015 167 676 777.6 0 676 168 430 777.6 0 430 169 524 777.6 0 524 170 367 777.6 0 367 171 595 777.6 0 595 172 485 777.6 0 485 173 1459 777.6 0 1459 174 1696 777.6 0 1696 175 1323 777.6 0 1323 176 580 777.6 0 580 177 264 777.6 0 264 178 295 777.6 0 295 179 357 777.6 0 357 180 827 777.6 0 827 181 1134 777.6 0 1134 182 895 777.6 0 895 183 591 777.6 0 591 184 510 777.6 0 510 185 896 777.6 0 896 186 1357 777.6 0 1357 187 980 777.6 0 980 188 584 777.6 0 584 189 369 777.6 0 369 190 619 777.6 0 619 191 1427 777.6 0 1427 192 985 777.6 0 985 193 1059 777.6 0 1059 194 525 777.6 0 525 195 358 777.6 0 358 196 310 777.6 0 310 197 303 777.6 0 303 198 726 777.6 0 726 199 835 777.6 0 835 200 635 777.6 0 635 201 805 777.6 0 805 202 541 777.6 0 541 203 761 777.6 0 761 204 727 777.6 0 727 205 773 777.6 0 773 206 613 777.6 0 613 207 770 777.6 0 770 208 587 777.6 0 587 209 354 777.6 0 354 210 817 777.6 0 817 211 665 777.6 0 665 212 560 777.6 0 560 213 600 777.6 0 600 214 626 777.6 0 626 215 708 777.6 0 708 216 560 777.6 0 560 217 598 777.6 0 598 218 901 777.6 0 901 219 1105 777.6 0 1105 220 987 777.6 0 987 221 851 777.6 0 851 222 390 777.6 0 390 223 282 777.6 0 282 224 730 777.6 0 730 225 780 777.6 0 780 226 747 777.6 0 747 227 940 777.6 0 940 228 966 777.6 0 966 229 755 777.6 0 755 230 648 777.6 0 648 231 670 777.6 0 670 232 959 777.6 0 959 233 749 777.6 0 749 234 510 777.6 0 510 235 741 777.6 0 741 236 752 777.6 0 752 237 634 777.6 0 634 238 535 777.6 0 535 239 652 777.6 0 652 240 701 777.6 0 701 241 652 777.6 0 652 242 550 777.6 0 550 243 482 777.6 0 482 244 643 777.6 0 643 245 1054 777.6 0 1054 246 1221 777.6 0 1221 247 1006 777.6 0 1006 248 637 777.6 0 637 249 382 777.6 0 382 250 391 777.6 0 391 251 465 777.6 0 465 252 895 777.6 0 895 253 880 777.6 0 880 254 1069 777.6 0 1069 255 1458 777.6 0 1458 256 1856 777.6 0 1856 257 1406 777.6 0 1406 258 1032 777.6 0 1032 259 655 777.6 0 655 260 726 777.6 0 726 261 582 777.6 0 582 262 456 777.6 0 456 263 503 777.6 0 503 264 474 777.6 0 474 265 761 777.6 0 761 266 1506 777.6 0 1506 267 1056 777.6 0 1056 268 1877 777.6 0 1877 269 1305 777.6 0 1305 270 994 777.6 0 994 271 1030 777.6 0 1030 272 1024 777.6 0 1024 273 957 777.6 0 957 274 1112 777.6 0 1112 275 1325 777.6 0 1325 276 5581 777.6 0 5581 277 5246 777.6 0 5246 278 2553 777.6 0 2553 279 1977 777.6 0 1977 280 1976 777.6 0 1976 281 1430 777.6 0 1430 282 1002 777.6 0 1002 283 596 777.6 0 596 284 936 777.6 0 936 285 1122 777.6 0 1122 286 1861 777.6 0 1861 287 1509 777.6 0 1509 288 1059 777.6 0 1059 289 689 777.6 0 689 290 829 777.6 0 829 291 565 777.6 0 565 292 1140 777.6 0 1140 293 1234 777.6 0 1234 294 2470 777.6 0 2470 295 8271 777.6 0 8271 296 4287 777.6 0 4287 297 5837 777.6 0 5837 298 964 777.6 0 964 299 1071 777.6 0 1071 300 697 777.6 0 697 301 490 777.6 0 490 Finished in 5.94 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 137,595 Reads with adapters: 1,837 (1.3%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 1,795 (1.3%) Total basepairs processed: 22,098,682 bp Total written (filtered): 490,150 bp (2.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1837 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 0 1 19 14 0.0 3 0 11 1 1 1 20 1767 0.0 4 1626 135 4 2 21 3 0.0 4 1 1 0 0 1 22 3 0.0 4 0 0 0 0 3 23 1 0.0 4 0 0 0 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 53 3 0.0 4 0 0 0 1 2 55 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 81 2 0.0 4 0 0 0 0 2 82 2 0.0 4 0 0 0 0 2 88 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 98 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 102 3 0.0 4 0 0 0 0 3 104 1 0.0 4 0 0 0 0 1 105 2 0.0 4 0 0 0 0 2 107 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 1,795 Reads with adapters: 24 (1.3%) Reads written (passing filters): 1,795 (100.0%) Total basepairs processed: 490,150 bp Total written (filtered): 489,743 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 24 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.0% C: 8.3% G: 87.5% T: 4.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 1 0.0 0 0 1 11 19 0.0 1 0 19 12 2 0.0 1 0 2 13 1 0.0 1 0 1 152 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.