This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.00 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 67,281 (2.1%) Reads that were too short: 34,761 (1.1%) Reads written (passing filters): 32,520 (1.0%) Total basepairs processed: 958,672,960 bp Total written (filtered): 6,144,479 bp (0.6%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 67281 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 311 777.6 0 311 7 105 777.6 0 105 8 280 777.6 0 280 9 8639 777.6 0 8639 10 19 777.6 0 19 11 11 777.6 0 11 12 2 777.6 0 2 13 12 777.6 0 12 14 14 777.6 0 14 15 22 777.6 0 22 16 5 777.6 0 5 17 4 777.6 0 4 18 8 777.6 0 8 19 161 777.6 0 161 20 84 777.6 0 84 21 7 777.6 0 7 22 1 777.6 0 1 23 2 777.6 0 2 24 4 777.6 0 4 25 12 777.6 0 12 26 15 777.6 0 15 27 19 777.6 0 19 28 3 777.6 0 3 29 2 777.6 0 2 30 1 777.6 0 1 31 2 777.6 0 2 32 2 777.6 0 2 33 8 777.6 0 8 34 5 777.6 0 5 35 3 777.6 0 3 36 2 777.6 0 2 37 2 777.6 0 2 38 1 777.6 0 1 40 3 777.6 0 3 41 7 777.6 0 7 42 4 777.6 0 4 43 34 777.6 0 34 44 39 777.6 0 39 45 75 777.6 0 75 46 9 777.6 0 9 47 7 777.6 0 7 48 3 777.6 0 3 49 3 777.6 0 3 50 14 777.6 0 14 51 3 777.6 0 3 52 7 777.6 0 7 53 6 777.6 0 6 54 3 777.6 0 3 55 1 777.6 0 1 56 3 777.6 0 3 57 1 777.6 0 1 58 3 777.6 0 3 59 9 777.6 0 9 60 14 777.6 0 14 61 7 777.6 0 7 62 5 777.6 0 5 63 6 777.6 0 6 64 5 777.6 0 5 65 4 777.6 0 4 66 8 777.6 0 8 67 16 777.6 0 16 68 20 777.6 0 20 69 10 777.6 0 10 70 19 777.6 0 19 71 177 777.6 0 177 72 99 777.6 0 99 73 18 777.6 0 18 74 37 777.6 0 37 75 43 777.6 0 43 76 158 777.6 0 158 77 875 777.6 0 875 78 1788 777.6 0 1788 79 701 777.6 0 701 80 30 777.6 0 30 81 22 777.6 0 22 82 106 777.6 0 106 83 52 777.6 0 52 84 89 777.6 0 89 85 33 777.6 0 33 86 14 777.6 0 14 87 14 777.6 0 14 88 36 777.6 0 36 89 2 777.6 0 2 90 27 777.6 0 27 91 143 777.6 0 143 92 115 777.6 0 115 93 30 777.6 0 30 94 39 777.6 0 39 95 33 777.6 0 33 96 22 777.6 0 22 97 33 777.6 0 33 98 29 777.6 0 29 99 13 777.6 0 13 100 8 777.6 0 8 101 6 777.6 0 6 102 6 777.6 0 6 103 10 777.6 0 10 104 14 777.6 0 14 105 2 777.6 0 2 106 3 777.6 0 3 107 32 777.6 0 32 108 21 777.6 0 21 109 9 777.6 0 9 110 1 777.6 0 1 111 7 777.6 0 7 112 5 777.6 0 5 113 21 777.6 0 21 114 55 777.6 0 55 115 75 777.6 0 75 116 71 777.6 0 71 117 30 777.6 0 30 118 15 777.6 0 15 119 13 777.6 0 13 120 22 777.6 0 22 121 30 777.6 0 30 122 37 777.6 0 37 123 71 777.6 0 71 124 36 777.6 0 36 125 30 777.6 0 30 126 39 777.6 0 39 127 24 777.6 0 24 128 35 777.6 0 35 129 44 777.6 0 44 130 36 777.6 0 36 131 17 777.6 0 17 132 26 777.6 0 26 133 18 777.6 0 18 134 58 777.6 0 58 135 127 777.6 0 127 136 141 777.6 0 141 137 249 777.6 0 249 138 555 777.6 0 555 139 65 777.6 0 65 140 16 777.6 0 16 141 198 777.6 0 198 142 35 777.6 0 35 143 82 777.6 0 82 144 28 777.6 0 28 145 24 777.6 0 24 146 21 777.6 0 21 147 11 777.6 0 11 148 19 777.6 0 19 149 33 777.6 0 33 150 45 777.6 0 45 151 34 777.6 0 34 152 18 777.6 0 18 153 13 777.6 0 13 154 19 777.6 0 19 155 44 777.6 0 44 156 37 777.6 0 37 157 62 777.6 0 62 158 74 777.6 0 74 159 22 777.6 0 22 160 25 777.6 0 25 161 24 777.6 0 24 162 22 777.6 0 22 163 36 777.6 0 36 164 104 777.6 0 104 165 37 777.6 0 37 166 46 777.6 0 46 167 31 777.6 0 31 168 41 777.6 0 41 169 20 777.6 0 20 170 25 777.6 0 25 171 96 777.6 0 96 172 77 777.6 0 77 173 162 777.6 0 162 174 304 777.6 0 304 175 1601 777.6 0 1601 176 121 777.6 0 121 177 80 777.6 0 80 178 77 777.6 0 77 179 47 777.6 0 47 180 227 777.6 0 227 181 175 777.6 0 175 182 297 777.6 0 297 183 69 777.6 0 69 184 57 777.6 0 57 185 36 777.6 0 36 186 37 777.6 0 37 187 84 777.6 0 84 188 116 777.6 0 116 189 307 777.6 0 307 190 554 777.6 0 554 191 2397 777.6 0 2397 192 4625 777.6 0 4625 193 2077 777.6 0 2077 194 268 777.6 0 268 195 40 777.6 0 40 196 68 777.6 0 68 197 47 777.6 0 47 198 16 777.6 0 16 199 52 777.6 0 52 200 61 777.6 0 61 201 141 777.6 0 141 202 1301 777.6 0 1301 203 200 777.6 0 200 204 186 777.6 0 186 205 224 777.6 0 224 206 27 777.6 0 27 207 28 777.6 0 28 208 199 777.6 0 199 209 101 777.6 0 101 210 119 777.6 0 119 211 66 777.6 0 66 212 24 777.6 0 24 213 17 777.6 0 17 214 5 777.6 0 5 215 23 777.6 0 23 216 30 777.6 0 30 217 22 777.6 0 22 218 24 777.6 0 24 219 9 777.6 0 9 220 23 777.6 0 23 221 28 777.6 0 28 222 16 777.6 0 16 223 6 777.6 0 6 224 5 777.6 0 5 225 31 777.6 0 31 226 43 777.6 0 43 227 36 777.6 0 36 228 21 777.6 0 21 229 28 777.6 0 28 230 14 777.6 0 14 231 11 777.6 0 11 232 32 777.6 0 32 233 45 777.6 0 45 234 33 777.6 0 33 235 16 777.6 0 16 236 35 777.6 0 35 237 26 777.6 0 26 238 16 777.6 0 16 239 17 777.6 0 17 240 14 777.6 0 14 241 21 777.6 0 21 242 18 777.6 0 18 243 24 777.6 0 24 244 21 777.6 0 21 245 18 777.6 0 18 246 41 777.6 0 41 247 86 777.6 0 86 248 106 777.6 0 106 249 91 777.6 0 91 250 111 777.6 0 111 251 81 777.6 0 81 252 95 777.6 0 95 253 54 777.6 0 54 254 92 777.6 0 92 255 84 777.6 0 84 256 39 777.6 0 39 257 123 777.6 0 123 258 229 777.6 0 229 259 1379 777.6 0 1379 260 4133 777.6 0 4133 261 2765 777.6 0 2765 262 1591 777.6 0 1591 263 950 777.6 0 950 264 393 777.6 0 393 265 301 777.6 0 301 266 173 777.6 0 173 267 27 777.6 0 27 268 83 777.6 0 83 269 892 777.6 0 892 270 464 777.6 0 464 271 355 777.6 0 355 272 110 777.6 0 110 273 89 777.6 0 89 274 100 777.6 0 100 275 266 777.6 0 266 276 506 777.6 0 506 277 395 777.6 0 395 278 59 777.6 0 59 279 69 777.6 0 69 280 102 777.6 0 102 281 218 777.6 0 218 282 277 777.6 0 277 283 553 777.6 0 553 284 2953 777.6 0 2953 285 2208 777.6 0 2208 286 3870 777.6 0 3870 287 132 777.6 0 132 288 258 777.6 0 258 289 1170 777.6 0 1170 290 1098 777.6 0 1098 291 355 777.6 0 355 292 31 777.6 0 31 293 12 777.6 0 12 294 56 777.6 0 56 295 421 777.6 0 421 296 375 777.6 0 375 297 905 777.6 0 905 298 31 777.6 0 31 299 17 777.6 0 17 300 13 777.6 0 13 301 171 777.6 0 171 Finished in 1.53 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 32,520 Reads with adapters: 8,882 (27.3%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 8,875 (27.3%) Total basepairs processed: 6,144,479 bp Total written (filtered): 2,414,494 bp (39.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8882 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 185 0.0 3 0 181 2 2 20 8655 0.0 4 8406 213 26 4 6 21 24 0.0 4 9 13 1 0 1 22 2 0.0 4 0 1 1 23 3 0.0 4 0 0 0 3 31 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 1 258 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 8,875 Reads with adapters: 546 (6.2%) Reads written (passing filters): 8,875 (100.0%) Total basepairs processed: 2,414,494 bp Total written (filtered): 2,408,950 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 546 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.8% C: 1.6% G: 92.9% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 8 1 0.1 0 1 9 138 0.0 0 2 136 10 187 0.0 1 6 181 11 218 0.0 1 1 217 13 2 0.0 1 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.