This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 102.43 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 72,121 (2.3%) Reads that were too short: 1,766 (0.1%) Reads written (passing filters): 70,355 (2.2%) Total basepairs processed: 958,672,960 bp Total written (filtered): 20,079,135 bp (2.1%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 72121 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4494 777.6 0 4494 7 1042 777.6 0 1042 8 10482 777.6 0 10482 9 48266 777.6 0 48266 10 61 777.6 0 61 11 9 777.6 0 9 12 6 777.6 0 6 13 9 777.6 0 9 14 6 777.6 0 6 15 7 777.6 0 7 16 3 777.6 0 3 17 4 777.6 0 4 18 12 777.6 0 12 19 6 777.6 0 6 20 13 777.6 0 13 21 4 777.6 0 4 22 8 777.6 0 8 23 11 777.6 0 11 24 7 777.6 0 7 25 4 777.6 0 4 26 8 777.6 0 8 27 5 777.6 0 5 28 14 777.6 0 14 29 6 777.6 0 6 30 13 777.6 0 13 31 9 777.6 0 9 32 6 777.6 0 6 33 7 777.6 0 7 34 9 777.6 0 9 35 12 777.6 0 12 36 20 777.6 0 20 37 26 777.6 0 26 38 57 777.6 0 57 39 23 777.6 0 23 40 39 777.6 0 39 41 922 777.6 0 922 42 92 777.6 0 92 43 21 777.6 0 21 44 94 777.6 0 94 45 71 777.6 0 71 46 6 777.6 0 6 47 6 777.6 0 6 48 6 777.6 0 6 49 8 777.6 0 8 50 4 777.6 0 4 51 4 777.6 0 4 52 6 777.6 0 6 53 14 777.6 0 14 54 4 777.6 0 4 55 3 777.6 0 3 56 10 777.6 0 10 57 14 777.6 0 14 58 114 777.6 0 114 59 40 777.6 0 40 60 64 777.6 0 64 61 8 777.6 0 8 62 9 777.6 0 9 63 19 777.6 0 19 64 7 777.6 0 7 65 3 777.6 0 3 66 12 777.6 0 12 67 5 777.6 0 5 68 6 777.6 0 6 69 10 777.6 0 10 70 29 777.6 0 29 71 14 777.6 0 14 72 10 777.6 0 10 73 6 777.6 0 6 74 6 777.6 0 6 75 12 777.6 0 12 76 373 777.6 0 373 77 203 777.6 0 203 78 211 777.6 0 211 79 11 777.6 0 11 80 270 777.6 0 270 81 41 777.6 0 41 82 44 777.6 0 44 83 20 777.6 0 20 84 7 777.6 0 7 86 1 777.6 0 1 87 12 777.6 0 12 88 45 777.6 0 45 89 2 777.6 0 2 90 20 777.6 0 20 91 10 777.6 0 10 92 4 777.6 0 4 93 3 777.6 0 3 94 7 777.6 0 7 95 10 777.6 0 10 96 22 777.6 0 22 97 90 777.6 0 90 98 153 777.6 0 153 99 11 777.6 0 11 100 88 777.6 0 88 101 132 777.6 0 132 102 255 777.6 0 255 103 22 777.6 0 22 104 16 777.6 0 16 105 10 777.6 0 10 106 4 777.6 0 4 107 5 777.6 0 5 108 10 777.6 0 10 109 15 777.6 0 15 110 149 777.6 0 149 111 127 777.6 0 127 112 445 777.6 0 445 113 3 777.6 0 3 114 4 777.6 0 4 115 4 777.6 0 4 116 8 777.6 0 8 117 5 777.6 0 5 118 22 777.6 0 22 119 5 777.6 0 5 120 2 777.6 0 2 121 8 777.6 0 8 122 6 777.6 0 6 123 6 777.6 0 6 124 5 777.6 0 5 125 6 777.6 0 6 126 2 777.6 0 2 127 27 777.6 0 27 128 3 777.6 0 3 129 5 777.6 0 5 130 2 777.6 0 2 131 8 777.6 0 8 132 8 777.6 0 8 133 7 777.6 0 7 134 12 777.6 0 12 135 7 777.6 0 7 136 11 777.6 0 11 137 7 777.6 0 7 138 14 777.6 0 14 139 10 777.6 0 10 140 6 777.6 0 6 141 8 777.6 0 8 142 15 777.6 0 15 143 8 777.6 0 8 144 3 777.6 0 3 145 1 777.6 0 1 146 4 777.6 0 4 147 14 777.6 0 14 148 6 777.6 0 6 150 5 777.6 0 5 151 13 777.6 0 13 152 8 777.6 0 8 153 3 777.6 0 3 154 4 777.6 0 4 155 2 777.6 0 2 156 4 777.6 0 4 157 37 777.6 0 37 158 17 777.6 0 17 159 13 777.6 0 13 160 8 777.6 0 8 161 7 777.6 0 7 162 12 777.6 0 12 163 10 777.6 0 10 164 6 777.6 0 6 165 6 777.6 0 6 166 4 777.6 0 4 167 18 777.6 0 18 168 200 777.6 0 200 169 63 777.6 0 63 170 13 777.6 0 13 171 12 777.6 0 12 172 5 777.6 0 5 173 4 777.6 0 4 174 3 777.6 0 3 175 3 777.6 0 3 176 6 777.6 0 6 177 9 777.6 0 9 178 8 777.6 0 8 179 6 777.6 0 6 180 6 777.6 0 6 181 8 777.6 0 8 182 19 777.6 0 19 183 14 777.6 0 14 184 25 777.6 0 25 185 8 777.6 0 8 186 5 777.6 0 5 187 11 777.6 0 11 188 14 777.6 0 14 189 10 777.6 0 10 190 4 777.6 0 4 191 5 777.6 0 5 192 8 777.6 0 8 193 13 777.6 0 13 194 17 777.6 0 17 195 28 777.6 0 28 196 12 777.6 0 12 197 5 777.6 0 5 198 16 777.6 0 16 199 20 777.6 0 20 200 64 777.6 0 64 201 47 777.6 0 47 202 4 777.6 0 4 203 12 777.6 0 12 204 53 777.6 0 53 205 44 777.6 0 44 206 42 777.6 0 42 207 19 777.6 0 19 208 14 777.6 0 14 209 4 777.6 0 4 210 8 777.6 0 8 211 9 777.6 0 9 212 17 777.6 0 17 213 26 777.6 0 26 214 41 777.6 0 41 215 30 777.6 0 30 216 30 777.6 0 30 217 32 777.6 0 32 218 29 777.6 0 29 219 24 777.6 0 24 220 21 777.6 0 21 221 23 777.6 0 23 222 33 777.6 0 33 223 29 777.6 0 29 224 46 777.6 0 46 225 28 777.6 0 28 226 13 777.6 0 13 227 18 777.6 0 18 228 20 777.6 0 20 229 26 777.6 0 26 230 24 777.6 0 24 231 8 777.6 0 8 232 7 777.6 0 7 233 11 777.6 0 11 234 8 777.6 0 8 235 9 777.6 0 9 236 13 777.6 0 13 237 18 777.6 0 18 238 20 777.6 0 20 239 14 777.6 0 14 240 18 777.6 0 18 241 29 777.6 0 29 242 13 777.6 0 13 243 10 777.6 0 10 244 12 777.6 0 12 245 12 777.6 0 12 246 7 777.6 0 7 247 10 777.6 0 10 248 13 777.6 0 13 249 27 777.6 0 27 250 15 777.6 0 15 251 8 777.6 0 8 252 6 777.6 0 6 253 20 777.6 0 20 254 21 777.6 0 21 255 37 777.6 0 37 256 21 777.6 0 21 257 26 777.6 0 26 258 19 777.6 0 19 259 18 777.6 0 18 260 17 777.6 0 17 261 3 777.6 0 3 262 10 777.6 0 10 263 23 777.6 0 23 264 32 777.6 0 32 265 26 777.6 0 26 266 14 777.6 0 14 267 10 777.6 0 10 268 18 777.6 0 18 269 16 777.6 0 16 270 4 777.6 0 4 271 3 777.6 0 3 272 9 777.6 0 9 273 16 777.6 0 16 274 18 777.6 0 18 275 10 777.6 0 10 276 21 777.6 0 21 277 19 777.6 0 19 278 13 777.6 0 13 279 17 777.6 0 17 280 15 777.6 0 15 281 30 777.6 0 30 282 17 777.6 0 17 283 11 777.6 0 11 284 9 777.6 0 9 285 16 777.6 0 16 286 11 777.6 0 11 287 17 777.6 0 17 288 32 777.6 0 32 289 21 777.6 0 21 290 11 777.6 0 11 291 5 777.6 0 5 292 7 777.6 0 7 293 6 777.6 0 6 294 18 777.6 0 18 295 19 777.6 0 19 296 13 777.6 0 13 297 9 777.6 0 9 298 7 777.6 0 7 299 9 777.6 0 9 300 8 777.6 0 8 301 7 777.6 0 7 Finished in 3.88 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 70,355 Reads with adapters: 13,105 (18.6%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 13,101 (18.6%) Total basepairs processed: 20,079,135 bp Total written (filtered): 3,619,010 bp (18.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13105 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 42 0.0 3 0 42 17 8848 0.0 3 8690 155 0 3 18 4155 0.0 3 4052 100 3 19 35 0.0 3 1 9 25 20 6 0.0 3 0 0 0 6 21 2 0.0 3 0 0 0 2 23 1 0.0 3 1 24 1 0.0 3 0 0 0 1 29 1 0.0 3 1 42 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.58 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 13,101 Reads with adapters: 458 (3.5%) Reads written (passing filters): 13,101 (100.0%) Total basepairs processed: 3,619,010 bp Total written (filtered): 3,617,633 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 458 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.6% C: 58.1% G: 23.6% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 455 204.7 0 455 4 3 51.2 0 3