This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 103.83 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 72,651 (2.3%) Reads that were too short: 22,490 (0.7%) Reads written (passing filters): 50,161 (1.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 12,640,937 bp (1.3%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 72651 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 240 777.6 0 240 7 672 777.6 0 672 8 32234 777.6 0 32234 9 23 777.6 0 23 10 2 777.6 0 2 11 4 777.6 0 4 12 6 777.6 0 6 13 3 777.6 0 3 14 6 777.6 0 6 15 8 777.6 0 8 16 6 777.6 0 6 17 7 777.6 0 7 18 61 777.6 0 61 19 29 777.6 0 29 20 15 777.6 0 15 21 5 777.6 0 5 22 3 777.6 0 3 23 6 777.6 0 6 24 2 777.6 0 2 25 3 777.6 0 3 26 4 777.6 0 4 27 16 777.6 0 16 28 13 777.6 0 13 29 3 777.6 0 3 30 12 777.6 0 12 31 17 777.6 0 17 32 5 777.6 0 5 33 17 777.6 0 17 34 43 777.6 0 43 35 10 777.6 0 10 36 81 777.6 0 81 37 193 777.6 0 193 38 132 777.6 0 132 39 17 777.6 0 17 40 13 777.6 0 13 41 11 777.6 0 11 42 30 777.6 0 30 43 832 777.6 0 832 44 284 777.6 0 284 45 235 777.6 0 235 46 14 777.6 0 14 47 1 777.6 0 1 48 1 777.6 0 1 49 3 777.6 0 3 50 2 777.6 0 2 51 4 777.6 0 4 52 4 777.6 0 4 53 3 777.6 0 3 54 5 777.6 0 5 55 7 777.6 0 7 56 6 777.6 0 6 57 18 777.6 0 18 58 3 777.6 0 3 59 3 777.6 0 3 60 2 777.6 0 2 61 5 777.6 0 5 62 2 777.6 0 2 63 6 777.6 0 6 64 4 777.6 0 4 65 1 777.6 0 1 66 2 777.6 0 2 67 4 777.6 0 4 68 3 777.6 0 3 69 3 777.6 0 3 70 6 777.6 0 6 71 18 777.6 0 18 72 7 777.6 0 7 73 11 777.6 0 11 74 12 777.6 0 12 75 141 777.6 0 141 76 45 777.6 0 45 77 25 777.6 0 25 78 56 777.6 0 56 79 79 777.6 0 79 80 66 777.6 0 66 81 94 777.6 0 94 82 115 777.6 0 115 83 79 777.6 0 79 84 113 777.6 0 113 85 43 777.6 0 43 86 19 777.6 0 19 87 45 777.6 0 45 88 99 777.6 0 99 89 52 777.6 0 52 90 42 777.6 0 42 91 315 777.6 0 315 92 64 777.6 0 64 93 119 777.6 0 119 94 247 777.6 0 247 95 93 777.6 0 93 96 58 777.6 0 58 97 62 777.6 0 62 98 47 777.6 0 47 99 165 777.6 0 165 100 30 777.6 0 30 101 33 777.6 0 33 102 13 777.6 0 13 103 10 777.6 0 10 104 11 777.6 0 11 105 17 777.6 0 17 106 11 777.6 0 11 107 25 777.6 0 25 108 51 777.6 0 51 109 49 777.6 0 49 110 20 777.6 0 20 111 31 777.6 0 31 112 30 777.6 0 30 113 56 777.6 0 56 114 48 777.6 0 48 115 43 777.6 0 43 116 87 777.6 0 87 117 75 777.6 0 75 118 30 777.6 0 30 119 37 777.6 0 37 120 128 777.6 0 128 121 119 777.6 0 119 122 113 777.6 0 113 123 105 777.6 0 105 124 55 777.6 0 55 125 69 777.6 0 69 126 79 777.6 0 79 127 115 777.6 0 115 128 127 777.6 0 127 129 221 777.6 0 221 130 184 777.6 0 184 131 141 777.6 0 141 132 335 777.6 0 335 133 170 777.6 0 170 134 127 777.6 0 127 135 790 777.6 0 790 136 504 777.6 0 504 137 944 777.6 0 944 138 107 777.6 0 107 139 137 777.6 0 137 140 191 777.6 0 191 141 127 777.6 0 127 142 352 777.6 0 352 143 132 777.6 0 132 144 134 777.6 0 134 145 129 777.6 0 129 146 103 777.6 0 103 147 80 777.6 0 80 148 144 777.6 0 144 149 266 777.6 0 266 150 123 777.6 0 123 151 82 777.6 0 82 152 58 777.6 0 58 153 47 777.6 0 47 154 76 777.6 0 76 155 143 777.6 0 143 156 143 777.6 0 143 157 92 777.6 0 92 158 78 777.6 0 78 159 96 777.6 0 96 160 86 777.6 0 86 161 83 777.6 0 83 162 72 777.6 0 72 163 79 777.6 0 79 164 90 777.6 0 90 165 113 777.6 0 113 166 135 777.6 0 135 167 135 777.6 0 135 168 137 777.6 0 137 169 59 777.6 0 59 170 35 777.6 0 35 171 43 777.6 0 43 172 80 777.6 0 80 173 60 777.6 0 60 174 93 777.6 0 93 175 52 777.6 0 52 176 61 777.6 0 61 177 50 777.6 0 50 178 86 777.6 0 86 179 96 777.6 0 96 180 162 777.6 0 162 181 144 777.6 0 144 182 144 777.6 0 144 183 102 777.6 0 102 184 97 777.6 0 97 185 89 777.6 0 89 186 84 777.6 0 84 187 105 777.6 0 105 188 214 777.6 0 214 189 223 777.6 0 223 190 189 777.6 0 189 191 148 777.6 0 148 192 159 777.6 0 159 193 80 777.6 0 80 194 70 777.6 0 70 195 82 777.6 0 82 196 324 777.6 0 324 197 141 777.6 0 141 198 73 777.6 0 73 199 44 777.6 0 44 200 50 777.6 0 50 201 173 777.6 0 173 202 484 777.6 0 484 203 328 777.6 0 328 204 282 777.6 0 282 205 71 777.6 0 71 206 42 777.6 0 42 207 121 777.6 0 121 208 242 777.6 0 242 209 333 777.6 0 333 210 216 777.6 0 216 211 315 777.6 0 315 212 266 777.6 0 266 213 220 777.6 0 220 214 174 777.6 0 174 215 435 777.6 0 435 216 235 777.6 0 235 217 176 777.6 0 176 218 91 777.6 0 91 219 44 777.6 0 44 220 80 777.6 0 80 221 178 777.6 0 178 222 342 777.6 0 342 223 155 777.6 0 155 224 179 777.6 0 179 225 254 777.6 0 254 226 142 777.6 0 142 227 88 777.6 0 88 228 148 777.6 0 148 229 83 777.6 0 83 230 78 777.6 0 78 231 51 777.6 0 51 232 135 777.6 0 135 233 167 777.6 0 167 234 178 777.6 0 178 235 297 777.6 0 297 236 200 777.6 0 200 237 84 777.6 0 84 238 68 777.6 0 68 239 48 777.6 0 48 240 55 777.6 0 55 241 124 777.6 0 124 242 190 777.6 0 190 243 285 777.6 0 285 244 205 777.6 0 205 245 116 777.6 0 116 246 71 777.6 0 71 247 51 777.6 0 51 248 50 777.6 0 50 249 52 777.6 0 52 250 104 777.6 0 104 251 174 777.6 0 174 252 136 777.6 0 136 253 93 777.6 0 93 254 83 777.6 0 83 255 108 777.6 0 108 256 112 777.6 0 112 257 70 777.6 0 70 258 44 777.6 0 44 259 80 777.6 0 80 260 69 777.6 0 69 261 61 777.6 0 61 262 96 777.6 0 96 263 132 777.6 0 132 264 245 777.6 0 245 265 214 777.6 0 214 266 295 777.6 0 295 267 107 777.6 0 107 268 62 777.6 0 62 269 351 777.6 0 351 270 531 777.6 0 531 271 488 777.6 0 488 272 221 777.6 0 221 273 183 777.6 0 183 274 206 777.6 0 206 275 182 777.6 0 182 276 155 777.6 0 155 277 142 777.6 0 142 278 178 777.6 0 178 279 825 777.6 0 825 280 260 777.6 0 260 281 192 777.6 0 192 282 162 777.6 0 162 283 160 777.6 0 160 284 323 777.6 0 323 285 146 777.6 0 146 286 203 777.6 0 203 287 472 777.6 0 472 288 212 777.6 0 212 289 136 777.6 0 136 290 184 777.6 0 184 291 310 777.6 0 310 292 300 777.6 0 300 293 151 777.6 0 151 294 346 777.6 0 346 295 1348 777.6 0 1348 296 785 777.6 0 785 297 405 777.6 0 405 298 718 777.6 0 718 299 523 777.6 0 523 300 1102 777.6 0 1102 301 76 777.6 0 76 Finished in 2.53 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 50,161 Reads with adapters: 33,017 (65.8%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 33,007 (65.8%) Total basepairs processed: 12,640,937 bp Total written (filtered): 9,010,751 bp (71.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 33017 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 14 0.0 3 0 0 11 3 19 458 0.0 3 0 412 33 9 4 20 32412 0.0 4 31237 1024 117 21 13 21 80 0.0 4 16 63 1 22 6 0.0 4 0 0 6 23 17 0.0 4 0 0 0 12 5 24 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 38 1 0.0 4 1 55 1 0.0 4 0 0 0 0 1 91 11 0.0 4 0 0 0 0 11 98 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 1 246 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.41 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 33,007 Reads with adapters: 4,704 (14.3%) Reads written (passing filters): 33,007 (100.0%) Total basepairs processed: 9,010,751 bp Total written (filtered): 8,931,920 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4704 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 4.5% G: 91.6% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 515.7 0 1 4 1 128.9 0 1 5 2 32.2 0 2 9 14 0.1 0 0 14 10 210 0.0 1 7 203 11 4328 0.0 1 12 4316 12 16 0.0 1 0 16 13 1 0.0 1 0 1 151 7 0.0 1 3 4 209 3 0.0 1 3 210 1 0.0 1 1 224 119 0.0 1 105 14 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.