This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 103.26 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 59,488 (1.9%) Reads that were too short: 9,741 (0.3%) Reads written (passing filters): 49,747 (1.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 10,809,664 bp (1.1%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 59488 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 467 777.6 0 467 7 25512 777.6 0 25512 8 19 777.6 0 19 9 30 777.6 0 30 10 16 777.6 0 16 11 12 777.6 0 12 12 12 777.6 0 12 13 18 777.6 0 18 14 24 777.6 0 24 15 16 777.6 0 16 16 25 777.6 0 25 17 60 777.6 0 60 18 44 777.6 0 44 19 22 777.6 0 22 20 17 777.6 0 17 21 17 777.6 0 17 22 11 777.6 0 11 23 23 777.6 0 23 24 14 777.6 0 14 25 17 777.6 0 17 26 13 777.6 0 13 27 15 777.6 0 15 28 21 777.6 0 21 29 21 777.6 0 21 30 19 777.6 0 19 31 14 777.6 0 14 32 17 777.6 0 17 33 16 777.6 0 16 34 13 777.6 0 13 35 22 777.6 0 22 36 15 777.6 0 15 37 15 777.6 0 15 38 11 777.6 0 11 39 15 777.6 0 15 40 16 777.6 0 16 41 31 777.6 0 31 42 146 777.6 0 146 43 54 777.6 0 54 44 82 777.6 0 82 45 12 777.6 0 12 46 12 777.6 0 12 47 11 777.6 0 11 48 14 777.6 0 14 49 10 777.6 0 10 50 171 777.6 0 171 51 20 777.6 0 20 52 16 777.6 0 16 53 4 777.6 0 4 54 14 777.6 0 14 55 15 777.6 0 15 56 16 777.6 0 16 57 6 777.6 0 6 58 14 777.6 0 14 59 11 777.6 0 11 60 10 777.6 0 10 61 9 777.6 0 9 62 17 777.6 0 17 63 13 777.6 0 13 64 10 777.6 0 10 65 24 777.6 0 24 66 17 777.6 0 17 67 13 777.6 0 13 68 9 777.6 0 9 69 8 777.6 0 8 70 9 777.6 0 9 71 22 777.6 0 22 72 8 777.6 0 8 73 37 777.6 0 37 74 42 777.6 0 42 75 51 777.6 0 51 76 13 777.6 0 13 77 20 777.6 0 20 78 6 777.6 0 6 79 76 777.6 0 76 80 52 777.6 0 52 81 31 777.6 0 31 82 10 777.6 0 10 83 13 777.6 0 13 84 16 777.6 0 16 85 10 777.6 0 10 86 86 777.6 0 86 87 30 777.6 0 30 88 12 777.6 0 12 89 19 777.6 0 19 90 20 777.6 0 20 91 19 777.6 0 19 92 384 777.6 0 384 93 390 777.6 0 390 94 328 777.6 0 328 95 43 777.6 0 43 96 38 777.6 0 38 97 19 777.6 0 19 98 11 777.6 0 11 99 13 777.6 0 13 100 6 777.6 0 6 101 9 777.6 0 9 102 13 777.6 0 13 103 12 777.6 0 12 104 7 777.6 0 7 105 10 777.6 0 10 106 17 777.6 0 17 107 15 777.6 0 15 108 11 777.6 0 11 109 29 777.6 0 29 110 29 777.6 0 29 111 18 777.6 0 18 112 18 777.6 0 18 113 28 777.6 0 28 114 98 777.6 0 98 115 17 777.6 0 17 116 14 777.6 0 14 117 24 777.6 0 24 118 28 777.6 0 28 119 24 777.6 0 24 120 44 777.6 0 44 121 34 777.6 0 34 122 59 777.6 0 59 123 967 777.6 0 967 124 630 777.6 0 630 125 643 777.6 0 643 126 18 777.6 0 18 127 24 777.6 0 24 128 24 777.6 0 24 129 22 777.6 0 22 130 28 777.6 0 28 131 185 777.6 0 185 132 40 777.6 0 40 133 9 777.6 0 9 134 17 777.6 0 17 135 15 777.6 0 15 136 19 777.6 0 19 137 12 777.6 0 12 138 16 777.6 0 16 139 26 777.6 0 26 140 23 777.6 0 23 141 37 777.6 0 37 142 11 777.6 0 11 143 11 777.6 0 11 144 32 777.6 0 32 145 13 777.6 0 13 146 26 777.6 0 26 147 74 777.6 0 74 148 49 777.6 0 49 149 7 777.6 0 7 150 12 777.6 0 12 151 11 777.6 0 11 152 1 777.6 0 1 153 9 777.6 0 9 154 11 777.6 0 11 155 17 777.6 0 17 156 20 777.6 0 20 157 53 777.6 0 53 158 16 777.6 0 16 159 28 777.6 0 28 160 27 777.6 0 27 161 24 777.6 0 24 162 18 777.6 0 18 163 29 777.6 0 29 164 19 777.6 0 19 165 16 777.6 0 16 166 34 777.6 0 34 167 59 777.6 0 59 168 27 777.6 0 27 169 33 777.6 0 33 170 46 777.6 0 46 171 13 777.6 0 13 172 43 777.6 0 43 173 52 777.6 0 52 174 303 777.6 0 303 175 343 777.6 0 343 176 135 777.6 0 135 177 53 777.6 0 53 178 84 777.6 0 84 179 121 777.6 0 121 180 130 777.6 0 130 181 85 777.6 0 85 182 68 777.6 0 68 183 38 777.6 0 38 184 117 777.6 0 117 185 62 777.6 0 62 186 139 777.6 0 139 187 109 777.6 0 109 188 84 777.6 0 84 189 12 777.6 0 12 190 14 777.6 0 14 191 21 777.6 0 21 192 61 777.6 0 61 193 82 777.6 0 82 194 297 777.6 0 297 195 204 777.6 0 204 196 27 777.6 0 27 197 31 777.6 0 31 198 873 777.6 0 873 199 3501 777.6 0 3501 200 9170 777.6 0 9170 201 187 777.6 0 187 202 29 777.6 0 29 203 19 777.6 0 19 204 29 777.6 0 29 205 64 777.6 0 64 206 72 777.6 0 72 207 31 777.6 0 31 208 11 777.6 0 11 209 15 777.6 0 15 210 17 777.6 0 17 211 20 777.6 0 20 212 30 777.6 0 30 213 24 777.6 0 24 214 226 777.6 0 226 215 22 777.6 0 22 216 19 777.6 0 19 217 17 777.6 0 17 218 35 777.6 0 35 219 319 777.6 0 319 220 338 777.6 0 338 221 159 777.6 0 159 222 163 777.6 0 163 223 115 777.6 0 115 224 52 777.6 0 52 225 27 777.6 0 27 226 45 777.6 0 45 227 48 777.6 0 48 228 68 777.6 0 68 229 20 777.6 0 20 230 20 777.6 0 20 231 11 777.6 0 11 232 12 777.6 0 12 233 12 777.6 0 12 234 16 777.6 0 16 235 34 777.6 0 34 236 67 777.6 0 67 237 84 777.6 0 84 238 180 777.6 0 180 239 335 777.6 0 335 240 1855 777.6 0 1855 241 1454 777.6 0 1454 242 125 777.6 0 125 243 15 777.6 0 15 244 16 777.6 0 16 245 41 777.6 0 41 246 140 777.6 0 140 247 120 777.6 0 120 248 217 777.6 0 217 249 68 777.6 0 68 250 39 777.6 0 39 251 6 777.6 0 6 252 26 777.6 0 26 253 15 777.6 0 15 254 34 777.6 0 34 255 62 777.6 0 62 256 177 777.6 0 177 257 257 777.6 0 257 258 258 777.6 0 258 259 26 777.6 0 26 260 17 777.6 0 17 261 470 777.6 0 470 262 22 777.6 0 22 263 24 777.6 0 24 264 12 777.6 0 12 265 15 777.6 0 15 266 19 777.6 0 19 267 41 777.6 0 41 268 48 777.6 0 48 269 22 777.6 0 22 270 23 777.6 0 23 271 8 777.6 0 8 272 12 777.6 0 12 273 10 777.6 0 10 274 21 777.6 0 21 275 30 777.6 0 30 276 10 777.6 0 10 277 8 777.6 0 8 278 11 777.6 0 11 279 35 777.6 0 35 280 43 777.6 0 43 281 34 777.6 0 34 282 14 777.6 0 14 283 15 777.6 0 15 284 16 777.6 0 16 285 100 777.6 0 100 286 223 777.6 0 223 287 208 777.6 0 208 288 130 777.6 0 130 289 32 777.6 0 32 290 60 777.6 0 60 291 44 777.6 0 44 292 56 777.6 0 56 293 38 777.6 0 38 294 18 777.6 0 18 295 14 777.6 0 14 296 16 777.6 0 16 297 29 777.6 0 29 298 9 777.6 0 9 299 16 777.6 0 16 300 5 777.6 0 5 301 7 777.6 0 7 Finished in 2.34 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 49,747 Reads with adapters: 25,823 (51.9%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 25,804 (51.9%) Total basepairs processed: 10,809,664 bp Total written (filtered): 7,070,123 bp (65.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 25823 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 20 0.0 3 0 0 16 2 2 19 289 0.0 3 0 273 9 7 20 25432 0.0 4 24803 553 61 13 2 21 33 0.0 4 3 27 2 0 1 22 4 0.0 4 0 1 2 0 1 23 18 0.0 4 0 0 0 16 2 28 1 0.0 4 0 1 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 1 53 2 0.0 4 0 0 0 0 2 58 2 0.0 4 0 0 0 1 1 62 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 86 4 0.0 4 0 0 0 0 4 91 5 0.0 4 0 0 0 0 5 92 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.10 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 25,804 Reads with adapters: 678 (2.6%) Reads written (passing filters): 25,804 (100.0%) Total basepairs processed: 7,070,123 bp Total written (filtered): 7,048,626 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 678 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.8% C: 13.3% G: 77.0% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 35 403.2 0 35 4 5 100.8 0 5 9 1 0.1 0 0 1 10 1 0.0 1 0 1 11 493 0.0 1 3 490 12 69 0.0 1 5 64 13 1 0.0 1 0 1 15 3 0.0 1 0 3 152 10 0.0 1 8 2 210 1 0.0 1 1 225 55 0.0 1 52 3 226 3 0.0 1 3 261 1 0.0 1 1