This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 104.35 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 75,076 (2.4%) Reads that were too short: 16,794 (0.5%) Reads written (passing filters): 58,282 (1.8%) Total basepairs processed: 958,672,960 bp Total written (filtered): 13,497,652 bp (1.4%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 75076 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 437 777.6 0 437 7 7602 777.6 0 7602 8 396 777.6 0 396 9 23191 777.6 0 23191 10 51 777.6 0 51 11 14 777.6 0 14 12 7 777.6 0 7 13 6 777.6 0 6 14 6 777.6 0 6 15 11 777.6 0 11 16 13 777.6 0 13 17 9 777.6 0 9 18 6 777.6 0 6 19 13 777.6 0 13 20 9 777.6 0 9 21 8 777.6 0 8 22 13 777.6 0 13 23 16 777.6 0 16 24 8 777.6 0 8 25 19 777.6 0 19 26 34 777.6 0 34 27 40 777.6 0 40 28 518 777.6 0 518 29 468 777.6 0 468 30 711 777.6 0 711 31 174 777.6 0 174 32 34 777.6 0 34 33 34 777.6 0 34 34 12 777.6 0 12 35 5 777.6 0 5 36 18 777.6 0 18 37 19 777.6 0 19 38 90 777.6 0 90 39 12 777.6 0 12 40 11 777.6 0 11 41 16 777.6 0 16 42 11 777.6 0 11 43 11 777.6 0 11 44 11 777.6 0 11 45 9 777.6 0 9 46 9 777.6 0 9 47 9 777.6 0 9 48 17 777.6 0 17 49 9 777.6 0 9 50 17 777.6 0 17 51 16 777.6 0 16 52 12 777.6 0 12 53 15 777.6 0 15 54 15 777.6 0 15 55 13 777.6 0 13 56 9 777.6 0 9 57 12 777.6 0 12 58 21 777.6 0 21 59 92 777.6 0 92 60 8 777.6 0 8 61 4 777.6 0 4 62 12 777.6 0 12 63 29 777.6 0 29 64 17 777.6 0 17 65 23 777.6 0 23 66 21 777.6 0 21 67 45 777.6 0 45 68 291 777.6 0 291 69 267 777.6 0 267 70 180 777.6 0 180 71 7 777.6 0 7 72 12 777.6 0 12 73 10 777.6 0 10 74 11 777.6 0 11 75 17 777.6 0 17 76 9 777.6 0 9 77 6 777.6 0 6 78 8 777.6 0 8 79 24 777.6 0 24 80 99 777.6 0 99 81 91 777.6 0 91 82 47 777.6 0 47 83 27 777.6 0 27 84 55 777.6 0 55 85 52 777.6 0 52 86 70 777.6 0 70 87 19 777.6 0 19 88 100 777.6 0 100 89 380 777.6 0 380 90 19 777.6 0 19 91 14 777.6 0 14 92 7 777.6 0 7 93 12 777.6 0 12 94 50 777.6 0 50 95 496 777.6 0 496 96 262 777.6 0 262 97 65 777.6 0 65 98 28 777.6 0 28 99 37 777.6 0 37 100 201 777.6 0 201 101 364 777.6 0 364 102 162 777.6 0 162 103 126 777.6 0 126 104 39 777.6 0 39 105 30 777.6 0 30 106 42 777.6 0 42 107 38 777.6 0 38 108 12 777.6 0 12 109 25 777.6 0 25 110 51 777.6 0 51 111 37 777.6 0 37 112 28 777.6 0 28 113 29 777.6 0 29 114 26 777.6 0 26 115 77 777.6 0 77 116 81 777.6 0 81 117 154 777.6 0 154 118 144 777.6 0 144 119 119 777.6 0 119 120 106 777.6 0 106 121 88 777.6 0 88 122 105 777.6 0 105 123 76 777.6 0 76 124 101 777.6 0 101 125 196 777.6 0 196 126 160 777.6 0 160 127 113 777.6 0 113 128 53 777.6 0 53 129 37 777.6 0 37 130 84 777.6 0 84 131 164 777.6 0 164 132 243 777.6 0 243 133 272 777.6 0 272 134 219 777.6 0 219 135 270 777.6 0 270 136 230 777.6 0 230 137 389 777.6 0 389 138 194 777.6 0 194 139 172 777.6 0 172 140 158 777.6 0 158 141 101 777.6 0 101 142 56 777.6 0 56 143 26 777.6 0 26 144 94 777.6 0 94 145 93 777.6 0 93 146 226 777.6 0 226 147 134 777.6 0 134 148 67 777.6 0 67 149 58 777.6 0 58 150 82 777.6 0 82 151 180 777.6 0 180 152 200 777.6 0 200 153 90 777.6 0 90 154 236 777.6 0 236 155 995 777.6 0 995 156 175 777.6 0 175 157 352 777.6 0 352 158 186 777.6 0 186 159 89 777.6 0 89 160 85 777.6 0 85 161 114 777.6 0 114 162 75 777.6 0 75 163 57 777.6 0 57 164 159 777.6 0 159 165 120 777.6 0 120 166 144 777.6 0 144 167 433 777.6 0 433 168 95 777.6 0 95 169 130 777.6 0 130 170 178 777.6 0 178 171 66 777.6 0 66 172 68 777.6 0 68 173 100 777.6 0 100 174 440 777.6 0 440 175 180 777.6 0 180 176 176 777.6 0 176 177 212 777.6 0 212 178 174 777.6 0 174 179 311 777.6 0 311 180 1554 777.6 0 1554 181 1201 777.6 0 1201 182 469 777.6 0 469 183 207 777.6 0 207 184 172 777.6 0 172 185 212 777.6 0 212 186 199 777.6 0 199 187 139 777.6 0 139 188 55 777.6 0 55 189 29 777.6 0 29 190 102 777.6 0 102 191 201 777.6 0 201 192 241 777.6 0 241 193 239 777.6 0 239 194 943 777.6 0 943 195 1043 777.6 0 1043 196 171 777.6 0 171 197 263 777.6 0 263 198 405 777.6 0 405 199 399 777.6 0 399 200 472 777.6 0 472 201 324 777.6 0 324 202 2385 777.6 0 2385 203 1415 777.6 0 1415 204 83 777.6 0 83 205 173 777.6 0 173 206 163 777.6 0 163 207 84 777.6 0 84 208 59 777.6 0 59 209 47 777.6 0 47 210 60 777.6 0 60 211 73 777.6 0 73 212 127 777.6 0 127 213 182 777.6 0 182 214 171 777.6 0 171 215 95 777.6 0 95 216 74 777.6 0 74 217 98 777.6 0 98 218 150 777.6 0 150 219 163 777.6 0 163 220 91 777.6 0 91 221 57 777.6 0 57 222 59 777.6 0 59 223 117 777.6 0 117 224 142 777.6 0 142 225 83 777.6 0 83 226 86 777.6 0 86 227 182 777.6 0 182 228 124 777.6 0 124 229 139 777.6 0 139 230 104 777.6 0 104 231 54 777.6 0 54 232 40 777.6 0 40 233 30 777.6 0 30 234 57 777.6 0 57 235 123 777.6 0 123 236 127 777.6 0 127 237 94 777.6 0 94 238 168 777.6 0 168 239 142 777.6 0 142 240 110 777.6 0 110 241 51 777.6 0 51 242 40 777.6 0 40 243 62 777.6 0 62 244 55 777.6 0 55 245 98 777.6 0 98 246 114 777.6 0 114 247 154 777.6 0 154 248 130 777.6 0 130 249 468 777.6 0 468 250 77 777.6 0 77 251 56 777.6 0 56 252 77 777.6 0 77 253 87 777.6 0 87 254 72 777.6 0 72 255 77 777.6 0 77 256 126 777.6 0 126 257 345 777.6 0 345 258 255 777.6 0 255 259 256 777.6 0 256 260 205 777.6 0 205 261 36 777.6 0 36 262 78 777.6 0 78 263 202 777.6 0 202 264 208 777.6 0 208 265 106 777.6 0 106 266 151 777.6 0 151 267 173 777.6 0 173 268 166 777.6 0 166 269 113 777.6 0 113 270 27 777.6 0 27 271 47 777.6 0 47 272 53 777.6 0 53 273 88 777.6 0 88 274 221 777.6 0 221 275 139 777.6 0 139 276 189 777.6 0 189 277 111 777.6 0 111 278 145 777.6 0 145 279 95 777.6 0 95 280 123 777.6 0 123 281 69 777.6 0 69 282 38 777.6 0 38 283 32 777.6 0 32 284 42 777.6 0 42 285 71 777.6 0 71 286 116 777.6 0 116 287 149 777.6 0 149 288 118 777.6 0 118 289 38 777.6 0 38 290 43 777.6 0 43 291 154 777.6 0 154 292 946 777.6 0 946 293 298 777.6 0 298 294 172 777.6 0 172 295 122 777.6 0 122 296 79 777.6 0 79 297 86 777.6 0 86 298 95 777.6 0 95 299 236 777.6 0 236 300 168 777.6 0 168 301 715 777.6 0 715 Finished in 2.66 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 58,282 Reads with adapters: 26,552 (45.6%) Reads that were too short: 37 (0.1%) Reads written (passing filters): 26,515 (45.5%) Total basepairs processed: 13,497,652 bp Total written (filtered): 7,287,742 bp (54.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26552 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 2 0.0 3 0 0 1 1 16 122 0.0 3 0 116 6 17 23767 0.0 3 23251 494 18 4 18 2529 0.0 3 2425 90 13 1 19 24 0.0 3 0 3 15 6 20 17 0.0 3 0 0 6 11 21 6 0.0 3 3 0 0 3 30 1 0.0 3 0 0 0 1 36 4 0.0 3 0 0 1 3 37 1 0.0 3 0 0 1 39 18 0.0 3 0 0 1 17 40 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 93 3 0.0 3 0 0 0 3 103 4 0.0 3 0 0 1 3 105 1 0.0 3 0 0 0 1 106 4 0.0 3 0 0 0 4 127 7 0.0 3 0 0 2 5 133 1 0.0 3 0 0 0 1 137 4 0.0 3 0 0 0 4 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 160 4 0.0 3 0 0 0 4 161 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 178 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 217 4 0.0 3 0 0 0 4 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.19 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 26,515 Reads with adapters: 839 (3.2%) Reads written (passing filters): 26,515 (100.0%) Total basepairs processed: 7,287,742 bp Total written (filtered): 7,285,212 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 839 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 51.0% C: 24.3% G: 22.9% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 826 414.3 0 826 4 13 103.6 0 13