This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.18 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 89,857 (2.8%) Reads that were too short: 26,933 (0.8%) Reads written (passing filters): 62,924 (2.0%) Total basepairs processed: 958,672,960 bp Total written (filtered): 13,671,114 bp (1.4%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 89857 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4807 777.6 0 4807 7 4787 777.6 0 4787 8 209 777.6 0 209 9 17746 777.6 0 17746 10 20 777.6 0 20 11 8 777.6 0 8 12 5 777.6 0 5 13 3 777.6 0 3 14 4 777.6 0 4 15 7 777.6 0 7 16 2 777.6 0 2 17 4 777.6 0 4 18 3 777.6 0 3 19 6 777.6 0 6 20 3 777.6 0 3 21 6 777.6 0 6 22 3 777.6 0 3 23 4 777.6 0 4 24 6 777.6 0 6 25 3 777.6 0 3 26 2 777.6 0 2 27 7 777.6 0 7 28 1 777.6 0 1 29 4 777.6 0 4 30 7 777.6 0 7 31 4 777.6 0 4 32 10 777.6 0 10 33 5 777.6 0 5 34 4 777.6 0 4 35 4 777.6 0 4 36 1 777.6 0 1 37 8 777.6 0 8 38 4 777.6 0 4 39 2 777.6 0 2 40 8 777.6 0 8 41 8 777.6 0 8 42 7 777.6 0 7 43 5 777.6 0 5 44 2 777.6 0 2 45 4 777.6 0 4 46 9 777.6 0 9 47 5 777.6 0 5 48 1 777.6 0 1 49 9 777.6 0 9 50 1009 777.6 0 1009 51 9 777.6 0 9 52 191 777.6 0 191 53 14 777.6 0 14 54 6 777.6 0 6 55 30 777.6 0 30 56 414 777.6 0 414 57 5 777.6 0 5 58 10 777.6 0 10 59 3 777.6 0 3 60 1 777.6 0 1 62 3 777.6 0 3 63 2 777.6 0 2 64 5 777.6 0 5 65 6 777.6 0 6 66 1 777.6 0 1 67 3 777.6 0 3 68 5 777.6 0 5 69 7 777.6 0 7 70 5 777.6 0 5 71 11 777.6 0 11 72 6 777.6 0 6 73 142 777.6 0 142 74 22 777.6 0 22 75 124 777.6 0 124 76 3 777.6 0 3 77 2 777.6 0 2 78 3 777.6 0 3 79 4 777.6 0 4 80 24 777.6 0 24 81 39 777.6 0 39 82 68 777.6 0 68 83 50 777.6 0 50 84 119 777.6 0 119 85 24 777.6 0 24 86 26 777.6 0 26 87 18 777.6 0 18 88 33 777.6 0 33 89 10 777.6 0 10 90 87 777.6 0 87 91 38 777.6 0 38 92 317 777.6 0 317 93 49 777.6 0 49 94 31 777.6 0 31 95 23 777.6 0 23 96 53 777.6 0 53 97 74 777.6 0 74 98 167 777.6 0 167 99 148 777.6 0 148 100 243 777.6 0 243 101 631 777.6 0 631 102 585 777.6 0 585 103 888 777.6 0 888 104 439 777.6 0 439 105 56 777.6 0 56 106 117 777.6 0 117 107 41 777.6 0 41 108 39 777.6 0 39 109 123 777.6 0 123 110 99 777.6 0 99 111 144 777.6 0 144 112 159 777.6 0 159 113 274 777.6 0 274 114 90 777.6 0 90 115 138 777.6 0 138 116 317 777.6 0 317 117 190 777.6 0 190 118 180 777.6 0 180 119 79 777.6 0 79 120 99 777.6 0 99 121 131 777.6 0 131 122 147 777.6 0 147 123 446 777.6 0 446 124 388 777.6 0 388 125 330 777.6 0 330 126 120 777.6 0 120 127 46 777.6 0 46 128 51 777.6 0 51 129 95 777.6 0 95 130 534 777.6 0 534 131 482 777.6 0 482 132 601 777.6 0 601 133 514 777.6 0 514 134 202 777.6 0 202 135 153 777.6 0 153 136 228 777.6 0 228 137 403 777.6 0 403 138 398 777.6 0 398 139 273 777.6 0 273 140 87 777.6 0 87 141 161 777.6 0 161 142 471 777.6 0 471 143 501 777.6 0 501 144 903 777.6 0 903 145 1042 777.6 0 1042 146 213 777.6 0 213 147 126 777.6 0 126 148 184 777.6 0 184 149 688 777.6 0 688 150 326 777.6 0 326 151 507 777.6 0 507 152 206 777.6 0 206 153 174 777.6 0 174 154 448 777.6 0 448 155 409 777.6 0 409 156 447 777.6 0 447 157 900 777.6 0 900 158 645 777.6 0 645 159 280 777.6 0 280 160 207 777.6 0 207 161 146 777.6 0 146 162 248 777.6 0 248 163 179 777.6 0 179 164 327 777.6 0 327 165 164 777.6 0 164 166 219 777.6 0 219 167 210 777.6 0 210 168 242 777.6 0 242 169 617 777.6 0 617 170 992 777.6 0 992 171 1280 777.6 0 1280 172 426 777.6 0 426 173 111 777.6 0 111 174 42 777.6 0 42 175 76 777.6 0 76 176 230 777.6 0 230 177 831 777.6 0 831 178 659 777.6 0 659 179 588 777.6 0 588 180 291 777.6 0 291 181 105 777.6 0 105 182 171 777.6 0 171 183 153 777.6 0 153 184 241 777.6 0 241 185 147 777.6 0 147 186 96 777.6 0 96 187 233 777.6 0 233 188 556 777.6 0 556 189 537 777.6 0 537 190 167 777.6 0 167 191 76 777.6 0 76 192 75 777.6 0 75 193 80 777.6 0 80 194 75 777.6 0 75 195 307 777.6 0 307 196 189 777.6 0 189 197 297 777.6 0 297 198 177 777.6 0 177 199 219 777.6 0 219 200 305 777.6 0 305 201 241 777.6 0 241 202 125 777.6 0 125 203 131 777.6 0 131 204 157 777.6 0 157 205 179 777.6 0 179 206 190 777.6 0 190 207 171 777.6 0 171 208 110 777.6 0 110 209 415 777.6 0 415 210 496 777.6 0 496 211 515 777.6 0 515 212 327 777.6 0 327 213 207 777.6 0 207 214 278 777.6 0 278 215 279 777.6 0 279 216 299 777.6 0 299 217 246 777.6 0 246 218 188 777.6 0 188 219 108 777.6 0 108 220 108 777.6 0 108 221 284 777.6 0 284 222 234 777.6 0 234 223 478 777.6 0 478 224 257 777.6 0 257 225 378 777.6 0 378 226 289 777.6 0 289 227 234 777.6 0 234 228 371 777.6 0 371 229 156 777.6 0 156 230 113 777.6 0 113 231 117 777.6 0 117 232 180 777.6 0 180 233 148 777.6 0 148 234 127 777.6 0 127 235 95 777.6 0 95 236 95 777.6 0 95 237 138 777.6 0 138 238 166 777.6 0 166 239 164 777.6 0 164 240 136 777.6 0 136 241 198 777.6 0 198 242 263 777.6 0 263 243 277 777.6 0 277 244 332 777.6 0 332 245 406 777.6 0 406 246 262 777.6 0 262 247 246 777.6 0 246 248 397 777.6 0 397 249 480 777.6 0 480 250 344 777.6 0 344 251 180 777.6 0 180 252 346 777.6 0 346 253 226 777.6 0 226 254 250 777.6 0 250 255 195 777.6 0 195 256 143 777.6 0 143 257 135 777.6 0 135 258 203 777.6 0 203 259 249 777.6 0 249 260 229 777.6 0 229 261 227 777.6 0 227 262 201 777.6 0 201 263 365 777.6 0 365 264 873 777.6 0 873 265 570 777.6 0 570 266 267 777.6 0 267 267 71 777.6 0 71 268 140 777.6 0 140 269 146 777.6 0 146 270 385 777.6 0 385 271 431 777.6 0 431 272 569 777.6 0 569 273 419 777.6 0 419 274 298 777.6 0 298 275 222 777.6 0 222 276 325 777.6 0 325 277 409 777.6 0 409 278 227 777.6 0 227 279 111 777.6 0 111 280 130 777.6 0 130 281 314 777.6 0 314 282 318 777.6 0 318 283 623 777.6 0 623 284 755 777.6 0 755 285 784 777.6 0 784 286 421 777.6 0 421 287 137 777.6 0 137 288 86 777.6 0 86 289 116 777.6 0 116 290 204 777.6 0 204 291 270 777.6 0 270 292 204 777.6 0 204 293 123 777.6 0 123 294 101 777.6 0 101 295 71 777.6 0 71 296 134 777.6 0 134 297 194 777.6 0 194 298 405 777.6 0 405 299 592 777.6 0 592 300 449 777.6 0 449 301 196 777.6 0 196 Finished in 2.80 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 62,924 Reads with adapters: 18,183 (28.9%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 18,171 (28.9%) Total basepairs processed: 13,671,114 bp Total written (filtered): 4,996,690 bp (36.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18183 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 75 0.0 3 0 73 2 17 18036 0.0 3 17622 401 12 1 18 26 0.0 3 5 20 1 19 12 0.0 3 0 0 9 3 20 12 0.0 3 0 0 0 12 21 1 0.0 3 0 0 0 1 28 1 0.0 3 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 47 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.84 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 18,171 Reads with adapters: 1,602 (8.8%) Reads written (passing filters): 18,171 (100.0%) Total basepairs processed: 4,996,690 bp Total written (filtered): 4,991,840 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1602 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 32.0% C: 15.5% G: 49.9% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1569 283.9 0 1569 4 26 71.0 0 26 5 4 17.7 0 4 6 2 4.4 0 2 7 1 1.1 0 1