This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 104.12 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 191,596 (6.0%) Reads that were too short: 13,578 (0.4%) Reads written (passing filters): 178,018 (5.6%) Total basepairs processed: 958,672,960 bp Total written (filtered): 37,567,670 bp (3.9%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 191596 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 109 777.6 0 109 7 2919 777.6 0 2919 8 229 777.6 0 229 9 6954 777.6 0 6954 10 35 777.6 0 35 11 19 777.6 0 19 12 18 777.6 0 18 13 10 777.6 0 10 14 17 777.6 0 17 15 11 777.6 0 11 16 4 777.6 0 4 17 5 777.6 0 5 18 7 777.6 0 7 19 10 777.6 0 10 20 10 777.6 0 10 21 16 777.6 0 16 22 10 777.6 0 10 23 11 777.6 0 11 24 12 777.6 0 12 25 9 777.6 0 9 26 14 777.6 0 14 27 12 777.6 0 12 28 9 777.6 0 9 29 10 777.6 0 10 30 6 777.6 0 6 31 7 777.6 0 7 32 18 777.6 0 18 33 8 777.6 0 8 34 26 777.6 0 26 35 43 777.6 0 43 36 38 777.6 0 38 37 13 777.6 0 13 38 10 777.6 0 10 39 10 777.6 0 10 40 12 777.6 0 12 41 9 777.6 0 9 42 9 777.6 0 9 43 8 777.6 0 8 44 15 777.6 0 15 45 7 777.6 0 7 46 13 777.6 0 13 47 11 777.6 0 11 48 45 777.6 0 45 49 13 777.6 0 13 50 23 777.6 0 23 51 26 777.6 0 26 52 15 777.6 0 15 53 28 777.6 0 28 54 101 777.6 0 101 55 196 777.6 0 196 56 17 777.6 0 17 57 16 777.6 0 16 58 10 777.6 0 10 59 10 777.6 0 10 60 14 777.6 0 14 61 33 777.6 0 33 62 18 777.6 0 18 63 48 777.6 0 48 64 110 777.6 0 110 65 108 777.6 0 108 66 62 777.6 0 62 67 44 777.6 0 44 68 59 777.6 0 59 69 84 777.6 0 84 70 69 777.6 0 69 71 53 777.6 0 53 72 27 777.6 0 27 73 43 777.6 0 43 74 56 777.6 0 56 75 69 777.6 0 69 76 201 777.6 0 201 77 283 777.6 0 283 78 662 777.6 0 662 79 1471 777.6 0 1471 80 2160 777.6 0 2160 81 85840 777.6 0 85840 82 8423 777.6 0 8423 83 17354 777.6 0 17354 84 158 777.6 0 158 85 101 777.6 0 101 86 169 777.6 0 169 87 233 777.6 0 233 88 41 777.6 0 41 89 57 777.6 0 57 90 18 777.6 0 18 91 21 777.6 0 21 92 83 777.6 0 83 93 71 777.6 0 71 94 38 777.6 0 38 95 28 777.6 0 28 96 40 777.6 0 40 97 40 777.6 0 40 98 84 777.6 0 84 99 133 777.6 0 133 100 36 777.6 0 36 101 21 777.6 0 21 102 112 777.6 0 112 103 70 777.6 0 70 104 101 777.6 0 101 105 85 777.6 0 85 106 170 777.6 0 170 107 143 777.6 0 143 108 224 777.6 0 224 109 511 777.6 0 511 110 93 777.6 0 93 111 141 777.6 0 141 112 271 777.6 0 271 113 128 777.6 0 128 114 147 777.6 0 147 115 247 777.6 0 247 116 161 777.6 0 161 117 324 777.6 0 324 118 256 777.6 0 256 119 339 777.6 0 339 120 567 777.6 0 567 121 614 777.6 0 614 122 13105 777.6 0 13105 123 10146 777.6 0 10146 124 11606 777.6 0 11606 125 113 777.6 0 113 126 101 777.6 0 101 127 36 777.6 0 36 128 33 777.6 0 33 129 81 777.6 0 81 130 81 777.6 0 81 131 88 777.6 0 88 132 30 777.6 0 30 133 66 777.6 0 66 134 45 777.6 0 45 135 67 777.6 0 67 136 42 777.6 0 42 137 47 777.6 0 47 138 63 777.6 0 63 139 56 777.6 0 56 140 44 777.6 0 44 141 41 777.6 0 41 142 45 777.6 0 45 143 127 777.6 0 127 144 658 777.6 0 658 145 427 777.6 0 427 146 393 777.6 0 393 147 52 777.6 0 52 148 38 777.6 0 38 149 30 777.6 0 30 150 35 777.6 0 35 151 128 777.6 0 128 152 136 777.6 0 136 153 66 777.6 0 66 154 66 777.6 0 66 155 84 777.6 0 84 156 61 777.6 0 61 157 68 777.6 0 68 158 58 777.6 0 58 159 61 777.6 0 61 160 40 777.6 0 40 161 46 777.6 0 46 162 40 777.6 0 40 163 56 777.6 0 56 164 58 777.6 0 58 165 51 777.6 0 51 166 49 777.6 0 49 167 42 777.6 0 42 168 57 777.6 0 57 169 61 777.6 0 61 170 71 777.6 0 71 171 215 777.6 0 215 172 77 777.6 0 77 173 55 777.6 0 55 174 25 777.6 0 25 175 18 777.6 0 18 176 34 777.6 0 34 177 81 777.6 0 81 178 82 777.6 0 82 179 89 777.6 0 89 180 56 777.6 0 56 181 74 777.6 0 74 182 1023 777.6 0 1023 183 464 777.6 0 464 184 1120 777.6 0 1120 185 59 777.6 0 59 186 22 777.6 0 22 187 21 777.6 0 21 188 53 777.6 0 53 189 123 777.6 0 123 190 245 777.6 0 245 191 45 777.6 0 45 192 19 777.6 0 19 193 28 777.6 0 28 194 40 777.6 0 40 195 44 777.6 0 44 196 91 777.6 0 91 197 89 777.6 0 89 198 48 777.6 0 48 199 27 777.6 0 27 200 28 777.6 0 28 201 38 777.6 0 38 202 66 777.6 0 66 203 51 777.6 0 51 204 29 777.6 0 29 205 66 777.6 0 66 206 34 777.6 0 34 207 72 777.6 0 72 208 58 777.6 0 58 209 51 777.6 0 51 210 65 777.6 0 65 211 49 777.6 0 49 212 34 777.6 0 34 213 39 777.6 0 39 214 51 777.6 0 51 215 46 777.6 0 46 216 58 777.6 0 58 217 62 777.6 0 62 218 39 777.6 0 39 219 28 777.6 0 28 220 35 777.6 0 35 221 55 777.6 0 55 222 128 777.6 0 128 223 89 777.6 0 89 224 41 777.6 0 41 225 48 777.6 0 48 226 48 777.6 0 48 227 42 777.6 0 42 228 49 777.6 0 49 229 17 777.6 0 17 230 23 777.6 0 23 231 29 777.6 0 29 232 42 777.6 0 42 233 68 777.6 0 68 234 73 777.6 0 73 235 32 777.6 0 32 236 37 777.6 0 37 237 36 777.6 0 36 238 60 777.6 0 60 239 74 777.6 0 74 240 1713 777.6 0 1713 241 428 777.6 0 428 242 658 777.6 0 658 243 88 777.6 0 88 244 40 777.6 0 40 245 55 777.6 0 55 246 38 777.6 0 38 247 19 777.6 0 19 248 25 777.6 0 25 249 47 777.6 0 47 250 63 777.6 0 63 251 134 777.6 0 134 252 115 777.6 0 115 253 120 777.6 0 120 254 52 777.6 0 52 255 45 777.6 0 45 256 28 777.6 0 28 257 21 777.6 0 21 258 15 777.6 0 15 259 83 777.6 0 83 260 76 777.6 0 76 261 437 777.6 0 437 262 390 777.6 0 390 263 395 777.6 0 395 264 263 777.6 0 263 265 175 777.6 0 175 266 105 777.6 0 105 267 60 777.6 0 60 268 156 777.6 0 156 269 55 777.6 0 55 270 254 777.6 0 254 271 65 777.6 0 65 272 69 777.6 0 69 273 55 777.6 0 55 274 58 777.6 0 58 275 165 777.6 0 165 276 197 777.6 0 197 277 104 777.6 0 104 278 77 777.6 0 77 279 53 777.6 0 53 280 82 777.6 0 82 281 99 777.6 0 99 282 122 777.6 0 122 283 283 777.6 0 283 284 39 777.6 0 39 285 60 777.6 0 60 286 49 777.6 0 49 287 64 777.6 0 64 288 160 777.6 0 160 289 114 777.6 0 114 290 292 777.6 0 292 291 893 777.6 0 893 292 569 777.6 0 569 293 155 777.6 0 155 294 167 777.6 0 167 295 283 777.6 0 283 296 619 777.6 0 619 297 261 777.6 0 261 298 130 777.6 0 130 299 87 777.6 0 87 300 64 777.6 0 64 301 66 777.6 0 66 Finished in 9.08 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 178,018 Reads with adapters: 10,031 (5.6%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 10,011 (5.6%) Total basepairs processed: 37,567,670 bp Total written (filtered): 2,723,122 bp (7.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10031 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 7 19 139 0.0 3 0 138 1 20 6950 0.0 4 6727 180 29 10 4 21 50 0.0 4 5 40 5 22 2856 0.0 4 2758 71 9 3 15 23 4 0.0 4 1 2 0 1 51 2 0.0 4 2 72 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 108 3 0.0 4 0 0 0 0 3 120 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 149 2 0.0 4 0 0 0 0 2 168 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 2 0.0 4 0 0 0 0 2 179 5 0.0 4 0 0 0 0 5 214 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.43 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 10,011 Reads with adapters: 896 (9.0%) Reads written (passing filters): 10,011 (100.0%) Total basepairs processed: 2,723,122 bp Total written (filtered): 2,698,376 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 896 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 2.9% G: 92.7% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 156.4 0 1 6 1 2.4 0 1 9 84 0.0 0 4 80 10 130 0.0 1 3 127 11 618 0.0 1 2 616 150 1 0.0 1 0 1 208 1 0.0 1 1 223 4 0.0 1 4 224 1 0.0 1 1 238 9 0.0 1 9 261 1 0.0 1 1 262 1 0.0 1 1 264 2 0.0 1 2 266 1 0.0 1 1 267 38 0.0 1 37 1 268 3 0.0 1 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.