This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 102.05 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,184,960 Reads with adapters: 107,972 (3.4%) Reads that were too short: 73,935 (2.3%) Reads written (passing filters): 34,037 (1.1%) Total basepairs processed: 958,672,960 bp Total written (filtered): 7,482,116 bp (0.8%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 107972 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 72 777.6 0 72 7 177 777.6 0 177 8 7161 777.6 0 7161 9 4014 777.6 0 4014 10 15 777.6 0 15 11 2 777.6 0 2 12 23 777.6 0 23 13 2 777.6 0 2 14 1 777.6 0 1 15 1 777.6 0 1 18 5 777.6 0 5 19 3 777.6 0 3 20 1 777.6 0 1 21 373 777.6 0 373 22 20 777.6 0 20 23 4 777.6 0 4 24 2 777.6 0 2 26 2 777.6 0 2 27 4 777.6 0 4 28 3 777.6 0 3 29 3 777.6 0 3 30 1 777.6 0 1 31 4 777.6 0 4 32 5 777.6 0 5 33 9 777.6 0 9 34 1 777.6 0 1 35 5 777.6 0 5 36 6 777.6 0 6 37 50 777.6 0 50 38 13 777.6 0 13 39 15 777.6 0 15 40 6 777.6 0 6 41 2 777.6 0 2 42 1 777.6 0 1 43 11 777.6 0 11 44 3 777.6 0 3 45 3 777.6 0 3 46 9 777.6 0 9 47 9 777.6 0 9 48 14 777.6 0 14 49 15 777.6 0 15 50 3 777.6 0 3 51 7 777.6 0 7 52 10 777.6 0 10 53 7 777.6 0 7 54 6 777.6 0 6 55 10 777.6 0 10 56 107 777.6 0 107 57 53 777.6 0 53 58 3256 777.6 0 3256 59 263 777.6 0 263 60 8 777.6 0 8 61 12 777.6 0 12 62 17 777.6 0 17 63 13 777.6 0 13 64 56 777.6 0 56 65 13 777.6 0 13 66 7 777.6 0 7 67 11 777.6 0 11 68 8 777.6 0 8 69 29 777.6 0 29 70 172 777.6 0 172 71 62 777.6 0 62 72 13 777.6 0 13 73 15 777.6 0 15 74 18 777.6 0 18 75 22 777.6 0 22 76 78 777.6 0 78 77 17 777.6 0 17 78 20 777.6 0 20 79 34 777.6 0 34 80 35 777.6 0 35 81 26 777.6 0 26 82 40 777.6 0 40 83 34 777.6 0 34 84 51 777.6 0 51 85 47 777.6 0 47 86 43 777.6 0 43 87 28 777.6 0 28 88 97 777.6 0 97 89 1912 777.6 0 1912 90 1347 777.6 0 1347 91 1629 777.6 0 1629 92 15 777.6 0 15 93 6 777.6 0 6 94 5 777.6 0 5 95 31 777.6 0 31 96 42 777.6 0 42 97 30 777.6 0 30 98 27 777.6 0 27 99 32 777.6 0 32 100 240 777.6 0 240 101 48 777.6 0 48 102 231 777.6 0 231 103 19 777.6 0 19 104 9 777.6 0 9 105 38 777.6 0 38 106 15 777.6 0 15 107 9 777.6 0 9 108 16 777.6 0 16 109 7 777.6 0 7 110 63 777.6 0 63 111 41 777.6 0 41 112 70 777.6 0 70 113 14 777.6 0 14 114 28 777.6 0 28 115 54 777.6 0 54 116 48 777.6 0 48 117 61 777.6 0 61 118 25 777.6 0 25 119 26 777.6 0 26 120 36 777.6 0 36 121 16 777.6 0 16 122 43 777.6 0 43 123 16 777.6 0 16 124 17 777.6 0 17 125 23 777.6 0 23 126 38 777.6 0 38 127 21 777.6 0 21 128 12 777.6 0 12 129 122 777.6 0 122 130 20 777.6 0 20 131 25 777.6 0 25 132 681 777.6 0 681 133 261 777.6 0 261 134 633 777.6 0 633 135 40 777.6 0 40 136 14 777.6 0 14 137 26 777.6 0 26 138 19 777.6 0 19 139 26 777.6 0 26 140 63 777.6 0 63 141 30 777.6 0 30 142 20 777.6 0 20 143 15 777.6 0 15 144 10 777.6 0 10 145 22 777.6 0 22 146 17 777.6 0 17 147 93 777.6 0 93 148 680 777.6 0 680 149 921 777.6 0 921 150 617 777.6 0 617 151 13 777.6 0 13 152 6 777.6 0 6 153 13 777.6 0 13 154 11 777.6 0 11 155 17 777.6 0 17 156 12 777.6 0 12 157 36 777.6 0 36 158 28 777.6 0 28 159 28 777.6 0 28 160 33 777.6 0 33 161 24 777.6 0 24 162 23 777.6 0 23 163 17 777.6 0 17 164 16 777.6 0 16 165 66 777.6 0 66 166 77 777.6 0 77 167 91 777.6 0 91 168 562 777.6 0 562 169 185 777.6 0 185 170 99 777.6 0 99 171 14 777.6 0 14 172 24 777.6 0 24 173 42 777.6 0 42 174 64 777.6 0 64 175 137 777.6 0 137 176 2536 777.6 0 2536 177 443 777.6 0 443 178 1332 777.6 0 1332 179 29 777.6 0 29 180 16 777.6 0 16 181 11 777.6 0 11 182 20 777.6 0 20 183 22 777.6 0 22 184 41 777.6 0 41 185 46 777.6 0 46 186 36 777.6 0 36 187 16 777.6 0 16 188 6 777.6 0 6 189 13 777.6 0 13 190 18 777.6 0 18 191 14 777.6 0 14 192 19 777.6 0 19 193 69 777.6 0 69 194 30 777.6 0 30 195 15 777.6 0 15 196 13 777.6 0 13 197 16 777.6 0 16 198 35 777.6 0 35 199 40 777.6 0 40 200 13 777.6 0 13 201 17 777.6 0 17 202 16 777.6 0 16 203 37 777.6 0 37 204 38 777.6 0 38 205 51 777.6 0 51 206 56 777.6 0 56 207 310 777.6 0 310 208 247 777.6 0 247 209 126 777.6 0 126 210 74 777.6 0 74 211 34 777.6 0 34 212 44 777.6 0 44 213 71 777.6 0 71 214 39 777.6 0 39 215 99 777.6 0 99 216 135 777.6 0 135 217 114 777.6 0 114 218 196 777.6 0 196 219 408 777.6 0 408 220 5759 777.6 0 5759 221 2755 777.6 0 2755 222 3175 777.6 0 3175 223 31 777.6 0 31 224 27 777.6 0 27 225 96 777.6 0 96 226 213 777.6 0 213 227 99 777.6 0 99 228 50 777.6 0 50 229 44 777.6 0 44 230 51 777.6 0 51 231 36 777.6 0 36 232 17 777.6 0 17 233 26 777.6 0 26 234 21 777.6 0 21 235 29 777.6 0 29 236 52 777.6 0 52 237 64 777.6 0 64 238 62 777.6 0 62 239 93 777.6 0 93 240 75 777.6 0 75 241 123 777.6 0 123 242 33 777.6 0 33 243 19 777.6 0 19 244 40 777.6 0 40 245 55 777.6 0 55 246 71 777.6 0 71 247 49 777.6 0 49 248 33 777.6 0 33 249 20 777.6 0 20 250 24 777.6 0 24 251 15 777.6 0 15 252 26 777.6 0 26 253 82 777.6 0 82 254 78 777.6 0 78 255 207 777.6 0 207 256 219 777.6 0 219 257 154 777.6 0 154 258 43 777.6 0 43 259 39 777.6 0 39 260 38 777.6 0 38 261 74 777.6 0 74 262 33 777.6 0 33 263 72 777.6 0 72 264 75 777.6 0 75 265 96 777.6 0 96 266 183 777.6 0 183 267 102 777.6 0 102 268 178 777.6 0 178 269 278 777.6 0 278 270 277 777.6 0 277 271 160 777.6 0 160 272 116 777.6 0 116 273 110 777.6 0 110 274 1190 777.6 0 1190 275 744 777.6 0 744 276 671 777.6 0 671 277 52 777.6 0 52 278 98 777.6 0 98 279 194 777.6 0 194 280 385 777.6 0 385 281 1370 777.6 0 1370 282 1960 777.6 0 1960 283 29047 777.6 0 29047 284 7016 777.6 0 7016 285 11671 777.6 0 11671 286 765 777.6 0 765 287 44 777.6 0 44 288 78 777.6 0 78 289 64 777.6 0 64 290 160 777.6 0 160 291 42 777.6 0 42 292 28 777.6 0 28 293 96 777.6 0 96 294 77 777.6 0 77 295 53 777.6 0 53 296 72 777.6 0 72 297 10 777.6 0 10 298 12 777.6 0 12 299 9 777.6 0 9 300 6 777.6 0 6 301 29 777.6 0 29 Finished in 1.55 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 34,037 Reads with adapters: 7,304 (21.5%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 7,297 (21.4%) Total basepairs processed: 7,482,116 bp Total written (filtered): 2,005,166 bp (26.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7304 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 28 0.0 3 0 28 17 7154 0.0 3 7069 83 2 18 23 0.0 3 1 19 2 1 19 20 0.0 3 0 0 16 4 20 15 0.0 3 0 0 0 15 22 1 0.0 3 1 43 11 0.0 3 0 0 0 11 45 2 0.0 3 2 46 20 0.0 3 0 0 0 20 74 1 0.0 3 1 76 1 0.0 3 1 102 11 0.0 3 0 0 0 11 105 8 0.0 3 0 0 0 8 124 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 202 4 0.0 3 0 0 0 4 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.32 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 7,297 Reads with adapters: 103 (1.4%) Reads written (passing filters): 7,297 (100.0%) Total basepairs processed: 2,005,166 bp Total written (filtered): 2,004,846 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 103 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 57.3% C: 20.4% G: 13.6% T: 8.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 92 114.0 0 92 4 11 28.5 0 11