This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 181.70 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 872,994 (16.4%) Reads that were too short: 398,255 (7.5%) Reads written (passing filters): 474,739 (8.9%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 75,322,505 bp (4.7%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 872994 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 68 1302.2 0 68 7 2663 1302.2 0 2663 8 22 1302.2 0 22 9 36 1302.2 0 36 10 42 1302.2 0 42 11 31 1302.2 0 31 12 33 1302.2 0 33 13 36 1302.2 0 36 14 33 1302.2 0 33 15 38 1302.2 0 38 16 32 1302.2 0 32 17 28 1302.2 0 28 18 28 1302.2 0 28 19 33 1302.2 0 33 20 35 1302.2 0 35 21 30 1302.2 0 30 22 33 1302.2 0 33 23 31 1302.2 0 31 24 23 1302.2 0 23 25 105 1302.2 0 105 26 139 1302.2 0 139 27 155 1302.2 0 155 28 50 1302.2 0 50 29 27 1302.2 0 27 30 32 1302.2 0 32 31 30 1302.2 0 30 32 28 1302.2 0 28 33 29 1302.2 0 29 34 40 1302.2 0 40 35 35 1302.2 0 35 36 36 1302.2 0 36 37 30 1302.2 0 30 38 32 1302.2 0 32 39 43 1302.2 0 43 40 28 1302.2 0 28 41 32 1302.2 0 32 42 39 1302.2 0 39 43 41 1302.2 0 41 44 27 1302.2 0 27 45 44 1302.2 0 44 46 29 1302.2 0 29 47 49 1302.2 0 49 48 32 1302.2 0 32 49 29 1302.2 0 29 50 30 1302.2 0 30 51 28 1302.2 0 28 52 28 1302.2 0 28 53 37 1302.2 0 37 54 34 1302.2 0 34 55 29 1302.2 0 29 56 36 1302.2 0 36 57 41 1302.2 0 41 58 30 1302.2 0 30 59 42 1302.2 0 42 60 44 1302.2 0 44 61 29 1302.2 0 29 62 19 1302.2 0 19 63 34 1302.2 0 34 64 31 1302.2 0 31 65 22 1302.2 0 22 66 31 1302.2 0 31 67 35 1302.2 0 35 68 35 1302.2 0 35 69 32 1302.2 0 32 70 28 1302.2 0 28 71 23 1302.2 0 23 72 31 1302.2 0 31 73 33 1302.2 0 33 74 46 1302.2 0 46 75 87 1302.2 0 87 76 266 1302.2 0 266 77 490 1302.2 0 490 78 2546 1302.2 0 2546 79 956 1302.2 0 956 80 1080 1302.2 0 1080 81 1911 1302.2 0 1911 82 2547 1302.2 0 2547 83 2587 1302.2 0 2587 84 2278 1302.2 0 2278 85 1648 1302.2 0 1648 86 1379 1302.2 0 1379 87 3615 1302.2 0 3615 88 5654 1302.2 0 5654 89 1144 1302.2 0 1144 90 4068 1302.2 0 4068 91 3140 1302.2 0 3140 92 716 1302.2 0 716 93 3346 1302.2 0 3346 94 6778 1302.2 0 6778 95 12011 1302.2 0 12011 96 2259 1302.2 0 2259 97 992 1302.2 0 992 98 1769 1302.2 0 1769 99 1094 1302.2 0 1094 100 785 1302.2 0 785 101 1753 1302.2 0 1753 102 1677 1302.2 0 1677 103 1154 1302.2 0 1154 104 3756 1302.2 0 3756 105 2935 1302.2 0 2935 106 1613 1302.2 0 1613 107 18166 1302.2 0 18166 108 4370 1302.2 0 4370 109 2466 1302.2 0 2466 110 1564 1302.2 0 1564 111 2170 1302.2 0 2170 112 1706 1302.2 0 1706 113 2023 1302.2 0 2023 114 2450 1302.2 0 2450 115 921 1302.2 0 921 116 1710 1302.2 0 1710 117 1215 1302.2 0 1215 118 866 1302.2 0 866 119 2599 1302.2 0 2599 120 4820 1302.2 0 4820 121 3554 1302.2 0 3554 122 3410 1302.2 0 3410 123 2627 1302.2 0 2627 124 1980 1302.2 0 1980 125 3036 1302.2 0 3036 126 4095 1302.2 0 4095 127 3779 1302.2 0 3779 128 3995 1302.2 0 3995 129 3513 1302.2 0 3513 130 1565 1302.2 0 1565 131 1882 1302.2 0 1882 132 2115 1302.2 0 2115 133 5090 1302.2 0 5090 134 8228 1302.2 0 8228 135 14245 1302.2 0 14245 136 12470 1302.2 0 12470 137 3979 1302.2 0 3979 138 2457 1302.2 0 2457 139 3214 1302.2 0 3214 140 6515 1302.2 0 6515 141 3566 1302.2 0 3566 142 3597 1302.2 0 3597 143 1711 1302.2 0 1711 144 2159 1302.2 0 2159 145 6635 1302.2 0 6635 146 5559 1302.2 0 5559 147 6958 1302.2 0 6958 148 4069 1302.2 0 4069 149 2896 1302.2 0 2896 150 2750 1302.2 0 2750 151 2297 1302.2 0 2297 152 5855 1302.2 0 5855 153 3416 1302.2 0 3416 154 4421 1302.2 0 4421 155 6144 1302.2 0 6144 156 5173 1302.2 0 5173 157 7094 1302.2 0 7094 158 6937 1302.2 0 6937 159 5771 1302.2 0 5771 160 6450 1302.2 0 6450 161 3704 1302.2 0 3704 162 3800 1302.2 0 3800 163 2481 1302.2 0 2481 164 3524 1302.2 0 3524 165 5227 1302.2 0 5227 166 5099 1302.2 0 5099 167 3990 1302.2 0 3990 168 2182 1302.2 0 2182 169 2775 1302.2 0 2775 170 1744 1302.2 0 1744 171 3043 1302.2 0 3043 172 2721 1302.2 0 2721 173 8462 1302.2 0 8462 174 9768 1302.2 0 9768 175 6277 1302.2 0 6277 176 2851 1302.2 0 2851 177 1231 1302.2 0 1231 178 1335 1302.2 0 1335 179 1761 1302.2 0 1761 180 5308 1302.2 0 5308 181 6136 1302.2 0 6136 182 4915 1302.2 0 4915 183 2987 1302.2 0 2987 184 2673 1302.2 0 2673 185 4589 1302.2 0 4589 186 7498 1302.2 0 7498 187 5470 1302.2 0 5470 188 3065 1302.2 0 3065 189 1789 1302.2 0 1789 190 3114 1302.2 0 3114 191 7136 1302.2 0 7136 192 6377 1302.2 0 6377 193 5387 1302.2 0 5387 194 2078 1302.2 0 2078 195 1896 1302.2 0 1896 196 1484 1302.2 0 1484 197 1521 1302.2 0 1521 198 3366 1302.2 0 3366 199 3132 1302.2 0 3132 200 2728 1302.2 0 2728 201 4584 1302.2 0 4584 202 3020 1302.2 0 3020 203 4706 1302.2 0 4706 204 4049 1302.2 0 4049 205 3903 1302.2 0 3903 206 2705 1302.2 0 2705 207 3825 1302.2 0 3825 208 3097 1302.2 0 3097 209 1970 1302.2 0 1970 210 2668 1302.2 0 2668 211 2111 1302.2 0 2111 212 3266 1302.2 0 3266 213 3975 1302.2 0 3975 214 3627 1302.2 0 3627 215 3820 1302.2 0 3820 216 3304 1302.2 0 3304 217 3194 1302.2 0 3194 218 4761 1302.2 0 4761 219 4757 1302.2 0 4757 220 4431 1302.2 0 4431 221 5828 1302.2 0 5828 222 2094 1302.2 0 2094 223 1355 1302.2 0 1355 224 3388 1302.2 0 3388 225 3286 1302.2 0 3286 226 3578 1302.2 0 3578 227 4084 1302.2 0 4084 228 4389 1302.2 0 4389 229 3891 1302.2 0 3891 230 3708 1302.2 0 3708 231 2778 1302.2 0 2778 232 4025 1302.2 0 4025 233 3142 1302.2 0 3142 234 2367 1302.2 0 2367 235 3501 1302.2 0 3501 236 3758 1302.2 0 3758 237 2650 1302.2 0 2650 238 2378 1302.2 0 2378 239 2762 1302.2 0 2762 240 3085 1302.2 0 3085 241 3183 1302.2 0 3183 242 2844 1302.2 0 2844 243 2355 1302.2 0 2355 244 3133 1302.2 0 3133 245 4086 1302.2 0 4086 246 4523 1302.2 0 4523 247 3497 1302.2 0 3497 248 2664 1302.2 0 2664 249 1178 1302.2 0 1178 250 1621 1302.2 0 1621 251 2460 1302.2 0 2460 252 6276 1302.2 0 6276 253 6559 1302.2 0 6559 254 4814 1302.2 0 4814 255 4979 1302.2 0 4979 256 7204 1302.2 0 7204 257 5531 1302.2 0 5531 258 4661 1302.2 0 4661 259 2942 1302.2 0 2942 260 3384 1302.2 0 3384 261 3358 1302.2 0 3358 262 3017 1302.2 0 3017 263 2614 1302.2 0 2614 264 2913 1302.2 0 2913 265 2505 1302.2 0 2505 266 5653 1302.2 0 5653 267 4843 1302.2 0 4843 268 6823 1302.2 0 6823 269 3698 1302.2 0 3698 270 2520 1302.2 0 2520 271 3024 1302.2 0 3024 272 2902 1302.2 0 2902 273 3251 1302.2 0 3251 274 3549 1302.2 0 3549 275 5013 1302.2 0 5013 276 8922 1302.2 0 8922 277 7122 1302.2 0 7122 278 5388 1302.2 0 5388 279 7639 1302.2 0 7639 280 6226 1302.2 0 6226 281 4825 1302.2 0 4825 282 2777 1302.2 0 2777 283 2133 1302.2 0 2133 284 4127 1302.2 0 4127 285 4596 1302.2 0 4596 286 5700 1302.2 0 5700 287 5489 1302.2 0 5489 288 4299 1302.2 0 4299 289 2911 1302.2 0 2911 290 1918 1302.2 0 1918 291 1609 1302.2 0 1609 292 2903 1302.2 0 2903 293 3912 1302.2 0 3912 294 6508 1302.2 0 6508 295 8404 1302.2 0 8404 296 15397 1302.2 0 15397 297 7449 1302.2 0 7449 298 1606 1302.2 0 1606 299 1742 1302.2 0 1742 300 2246 1302.2 0 2246 301 5594 1302.2 0 5594 Finished in 17.11 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 474,739 Reads with adapters: 2,743 (0.6%) Reads that were too short: 49 (0.0%) Reads written (passing filters): 2,694 (0.6%) Total basepairs processed: 75,322,505 bp Total written (filtered): 736,950 bp (1.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2743 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 11 0.0 3 0 8 2 1 17 2607 0.0 3 2227 356 24 18 12 0.0 3 0 3 8 1 19 3 0.0 3 0 0 2 1 20 5 0.0 3 0 0 0 5 27 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 9 0.0 3 0 0 0 9 35 4 0.0 3 0 0 0 4 36 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 8 0.0 3 0 0 0 8 59 2 0.0 3 0 0 2 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 9 0.0 3 0 0 0 9 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 1 1 104 2 0.0 3 0 0 0 2 105 3 0.0 3 0 0 0 3 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 135 4 0.0 3 0 0 0 4 137 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 202 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 2,694 Reads with adapters: 177 (6.6%) Reads written (passing filters): 2,694 (100.0%) Total basepairs processed: 736,950 bp Total written (filtered): 736,419 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 177 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.0% C: 72.3% G: 10.7% T: 4.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 177 42.1 0 177