This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 168.23 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 117,911 (2.2%) Reads that were too short: 8,973 (0.2%) Reads written (passing filters): 108,938 (2.0%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 28,711,307 bp (1.8%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 117911 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 823 1302.2 0 823 7 1430 1302.2 0 1430 8 2441 1302.2 0 2441 9 78666 1302.2 0 78666 10 148 1302.2 0 148 11 10 1302.2 0 10 12 17 1302.2 0 17 13 10 1302.2 0 10 14 13 1302.2 0 13 15 14 1302.2 0 14 16 16 1302.2 0 16 17 11 1302.2 0 11 18 25 1302.2 0 25 19 11 1302.2 0 11 20 8 1302.2 0 8 21 9 1302.2 0 9 22 7 1302.2 0 7 23 13 1302.2 0 13 24 10 1302.2 0 10 25 8 1302.2 0 8 26 12 1302.2 0 12 27 12 1302.2 0 12 28 19 1302.2 0 19 29 42 1302.2 0 42 30 19 1302.2 0 19 31 32 1302.2 0 32 32 110 1302.2 0 110 33 85 1302.2 0 85 34 108 1302.2 0 108 35 37 1302.2 0 37 36 26 1302.2 0 26 37 110 1302.2 0 110 38 81 1302.2 0 81 39 27 1302.2 0 27 40 26 1302.2 0 26 41 305 1302.2 0 305 42 114 1302.2 0 114 43 6 1302.2 0 6 44 11 1302.2 0 11 45 10 1302.2 0 10 46 26 1302.2 0 26 47 15 1302.2 0 15 48 21 1302.2 0 21 49 35 1302.2 0 35 50 15 1302.2 0 15 51 30 1302.2 0 30 52 1804 1302.2 0 1804 53 20 1302.2 0 20 54 25 1302.2 0 25 55 13 1302.2 0 13 56 10 1302.2 0 10 57 12 1302.2 0 12 58 8 1302.2 0 8 59 9 1302.2 0 9 60 22 1302.2 0 22 61 24 1302.2 0 24 62 12 1302.2 0 12 63 7 1302.2 0 7 64 6 1302.2 0 6 65 19 1302.2 0 19 66 3 1302.2 0 3 67 17 1302.2 0 17 68 9 1302.2 0 9 69 3 1302.2 0 3 70 11 1302.2 0 11 71 15 1302.2 0 15 72 91 1302.2 0 91 73 126 1302.2 0 126 74 9 1302.2 0 9 75 9 1302.2 0 9 76 7 1302.2 0 7 77 9 1302.2 0 9 78 11 1302.2 0 11 79 25 1302.2 0 25 80 16 1302.2 0 16 81 115 1302.2 0 115 82 97 1302.2 0 97 83 51 1302.2 0 51 84 244 1302.2 0 244 85 120 1302.2 0 120 86 193 1302.2 0 193 87 29 1302.2 0 29 88 22 1302.2 0 22 89 30 1302.2 0 30 90 89 1302.2 0 89 91 107 1302.2 0 107 92 149 1302.2 0 149 93 199 1302.2 0 199 94 353 1302.2 0 353 95 309 1302.2 0 309 96 17 1302.2 0 17 97 34 1302.2 0 34 98 14 1302.2 0 14 99 12 1302.2 0 12 100 8 1302.2 0 8 101 13 1302.2 0 13 102 28 1302.2 0 28 103 20 1302.2 0 20 104 51 1302.2 0 51 105 23 1302.2 0 23 106 20 1302.2 0 20 107 14 1302.2 0 14 108 89 1302.2 0 89 109 52 1302.2 0 52 110 72 1302.2 0 72 111 28 1302.2 0 28 112 66 1302.2 0 66 113 48 1302.2 0 48 114 15 1302.2 0 15 115 45 1302.2 0 45 116 42 1302.2 0 42 117 124 1302.2 0 124 118 86 1302.2 0 86 119 64 1302.2 0 64 120 120 1302.2 0 120 121 72 1302.2 0 72 122 88 1302.2 0 88 123 81 1302.2 0 81 124 108 1302.2 0 108 125 111 1302.2 0 111 126 98 1302.2 0 98 127 415 1302.2 0 415 128 116 1302.2 0 116 129 174 1302.2 0 174 130 53 1302.2 0 53 131 92 1302.2 0 92 132 121 1302.2 0 121 133 142 1302.2 0 142 134 104 1302.2 0 104 135 79 1302.2 0 79 136 57 1302.2 0 57 137 49 1302.2 0 49 138 73 1302.2 0 73 139 119 1302.2 0 119 140 123 1302.2 0 123 141 143 1302.2 0 143 142 195 1302.2 0 195 143 288 1302.2 0 288 144 4415 1302.2 0 4415 145 1436 1302.2 0 1436 146 2287 1302.2 0 2287 147 49 1302.2 0 49 148 71 1302.2 0 71 149 36 1302.2 0 36 150 37 1302.2 0 37 151 348 1302.2 0 348 152 210 1302.2 0 210 153 198 1302.2 0 198 154 119 1302.2 0 119 155 119 1302.2 0 119 156 182 1302.2 0 182 157 267 1302.2 0 267 158 122 1302.2 0 122 159 135 1302.2 0 135 160 74 1302.2 0 74 161 108 1302.2 0 108 162 62 1302.2 0 62 163 66 1302.2 0 66 164 81 1302.2 0 81 165 145 1302.2 0 145 166 149 1302.2 0 149 167 96 1302.2 0 96 168 69 1302.2 0 69 169 47 1302.2 0 47 170 131 1302.2 0 131 171 164 1302.2 0 164 172 330 1302.2 0 330 173 277 1302.2 0 277 174 100 1302.2 0 100 175 66 1302.2 0 66 176 78 1302.2 0 78 177 124 1302.2 0 124 178 118 1302.2 0 118 179 239 1302.2 0 239 180 130 1302.2 0 130 181 159 1302.2 0 159 182 100 1302.2 0 100 183 57 1302.2 0 57 184 87 1302.2 0 87 185 159 1302.2 0 159 186 88 1302.2 0 88 187 34 1302.2 0 34 188 33 1302.2 0 33 189 51 1302.2 0 51 190 389 1302.2 0 389 191 139 1302.2 0 139 192 251 1302.2 0 251 193 78 1302.2 0 78 194 84 1302.2 0 84 195 54 1302.2 0 54 196 153 1302.2 0 153 197 280 1302.2 0 280 198 368 1302.2 0 368 199 106 1302.2 0 106 200 52 1302.2 0 52 201 42 1302.2 0 42 202 31 1302.2 0 31 203 46 1302.2 0 46 204 48 1302.2 0 48 205 94 1302.2 0 94 206 87 1302.2 0 87 207 200 1302.2 0 200 208 86 1302.2 0 86 209 28 1302.2 0 28 210 35 1302.2 0 35 211 61 1302.2 0 61 212 81 1302.2 0 81 213 78 1302.2 0 78 214 73 1302.2 0 73 215 40 1302.2 0 40 216 69 1302.2 0 69 217 287 1302.2 0 287 218 100 1302.2 0 100 219 402 1302.2 0 402 220 47 1302.2 0 47 221 36 1302.2 0 36 222 33 1302.2 0 33 223 22 1302.2 0 22 224 46 1302.2 0 46 225 131 1302.2 0 131 226 177 1302.2 0 177 227 93 1302.2 0 93 228 87 1302.2 0 87 229 70 1302.2 0 70 230 51 1302.2 0 51 231 35 1302.2 0 35 232 38 1302.2 0 38 233 34 1302.2 0 34 234 20 1302.2 0 20 235 39 1302.2 0 39 236 194 1302.2 0 194 237 89 1302.2 0 89 238 70 1302.2 0 70 239 11 1302.2 0 11 240 71 1302.2 0 71 241 51 1302.2 0 51 242 40 1302.2 0 40 243 41 1302.2 0 41 244 37 1302.2 0 37 245 118 1302.2 0 118 246 148 1302.2 0 148 247 124 1302.2 0 124 248 93 1302.2 0 93 249 40 1302.2 0 40 250 33 1302.2 0 33 251 46 1302.2 0 46 252 113 1302.2 0 113 253 145 1302.2 0 145 254 317 1302.2 0 317 255 237 1302.2 0 237 256 225 1302.2 0 225 257 46 1302.2 0 46 258 27 1302.2 0 27 259 23 1302.2 0 23 260 61 1302.2 0 61 261 102 1302.2 0 102 262 147 1302.2 0 147 263 175 1302.2 0 175 264 87 1302.2 0 87 265 85 1302.2 0 85 266 63 1302.2 0 63 267 116 1302.2 0 116 268 58 1302.2 0 58 269 42 1302.2 0 42 270 65 1302.2 0 65 271 105 1302.2 0 105 272 102 1302.2 0 102 273 24 1302.2 0 24 274 24 1302.2 0 24 275 18 1302.2 0 18 276 20 1302.2 0 20 277 64 1302.2 0 64 278 73 1302.2 0 73 279 44 1302.2 0 44 280 52 1302.2 0 52 281 33 1302.2 0 33 282 46 1302.2 0 46 283 213 1302.2 0 213 284 112 1302.2 0 112 285 628 1302.2 0 628 286 51 1302.2 0 51 287 18 1302.2 0 18 288 91 1302.2 0 91 289 20 1302.2 0 20 290 96 1302.2 0 96 291 367 1302.2 0 367 292 219 1302.2 0 219 293 163 1302.2 0 163 294 102 1302.2 0 102 295 45 1302.2 0 45 296 30 1302.2 0 30 297 27 1302.2 0 27 298 12 1302.2 0 12 299 9 1302.2 0 9 300 10 1302.2 0 10 301 10 1302.2 0 10 Finished in 5.47 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 108,938 Reads with adapters: 82,931 (76.1%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 82,926 (76.1%) Total basepairs processed: 28,711,307 bp Total written (filtered): 22,562,952 bp (78.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 82931 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 3 2 18 45 0.0 3 0 0 37 4 4 19 1182 0.0 3 0 1101 38 18 25 20 81386 0.0 4 78641 2202 348 106 89 21 188 0.0 4 22 144 5 16 1 22 48 0.0 4 3 1 20 3 21 23 60 0.0 4 6 0 1 39 14 24 5 0.0 4 0 0 0 2 3 26 1 0.0 4 1 28 1 0.0 4 0 0 0 1 36 1 0.0 4 0 0 0 0 1 37 1 0.0 4 1 43 1 0.0 4 1 46 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 1 104 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.56 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 82,926 Reads with adapters: 13,992 (16.9%) Reads written (passing filters): 82,926 (100.0%) Total basepairs processed: 22,562,952 bp Total written (filtered): 20,837,715 bp (92.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 13992 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 47.4% C: 1.4% G: 50.1% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 1295.7 0 4 4 7 323.9 0 7 5 5 81.0 0 5 6 6 20.2 0 6 8 8 1.3 0 8 9 1281 0.3 0 103 1178 10 1099 0.1 1 41 1058 11 5153 0.0 1 16 5137 12 15 0.0 1 0 15 13 10 0.0 1 0 10 14 1 0.0 1 0 1 150 5 0.0 1 5 189 1 0.0 1 0 1 223 1 0.0 1 0 1 233 1 0.0 1 0 1 255 4 0.0 1 4 256 24 0.0 1 24 257 5975 0.0 1 5614 361 258 226 0.0 1 206 20 259 97 0.0 1 95 2 260 64 0.0 1 61 3 261 1 0.0 1 1 265 1 0.0 1 1 271 2 0.0 1 2 273 1 0.0 1 1