This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 176.78 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 121,990 (2.3%) Reads that were too short: 54,155 (1.0%) Reads written (passing filters): 67,835 (1.3%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 13,695,561 bp (0.9%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 121990 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 288 1302.2 0 288 7 20390 1302.2 0 20390 8 80 1302.2 0 80 9 7 1302.2 0 7 10 6 1302.2 0 6 11 4 1302.2 0 4 12 7 1302.2 0 7 13 4 1302.2 0 4 14 6 1302.2 0 6 15 3 1302.2 0 3 16 7 1302.2 0 7 17 11 1302.2 0 11 18 21 1302.2 0 21 19 8 1302.2 0 8 20 12 1302.2 0 12 21 1 1302.2 0 1 22 4 1302.2 0 4 23 3 1302.2 0 3 24 3 1302.2 0 3 25 6 1302.2 0 6 26 4 1302.2 0 4 27 4 1302.2 0 4 28 5 1302.2 0 5 29 7 1302.2 0 7 30 5 1302.2 0 5 31 6 1302.2 0 6 32 8 1302.2 0 8 33 9 1302.2 0 9 34 6 1302.2 0 6 35 8 1302.2 0 8 36 349 1302.2 0 349 37 52 1302.2 0 52 38 1613 1302.2 0 1613 39 221 1302.2 0 221 40 8 1302.2 0 8 41 12 1302.2 0 12 42 11 1302.2 0 11 43 19 1302.2 0 19 44 144 1302.2 0 144 45 64 1302.2 0 64 46 6 1302.2 0 6 47 10 1302.2 0 10 48 6 1302.2 0 6 49 10 1302.2 0 10 50 6 1302.2 0 6 51 5 1302.2 0 5 52 7 1302.2 0 7 53 12 1302.2 0 12 54 11 1302.2 0 11 55 13 1302.2 0 13 56 9 1302.2 0 9 57 7 1302.2 0 7 58 9 1302.2 0 9 59 9 1302.2 0 9 60 11 1302.2 0 11 61 13 1302.2 0 13 62 5 1302.2 0 5 63 6 1302.2 0 6 64 3 1302.2 0 3 65 2 1302.2 0 2 66 1 1302.2 0 1 67 1 1302.2 0 1 68 11 1302.2 0 11 69 7 1302.2 0 7 70 17 1302.2 0 17 71 7 1302.2 0 7 72 2 1302.2 0 2 73 5 1302.2 0 5 74 16 1302.2 0 16 75 4 1302.2 0 4 76 19 1302.2 0 19 77 27 1302.2 0 27 78 74 1302.2 0 74 79 156 1302.2 0 156 80 99 1302.2 0 99 81 41 1302.2 0 41 82 87 1302.2 0 87 83 67 1302.2 0 67 84 84 1302.2 0 84 85 24 1302.2 0 24 86 4 1302.2 0 4 87 63 1302.2 0 63 88 112 1302.2 0 112 89 49 1302.2 0 49 90 24 1302.2 0 24 91 225 1302.2 0 225 92 128 1302.2 0 128 93 411 1302.2 0 411 94 896 1302.2 0 896 95 382 1302.2 0 382 96 139 1302.2 0 139 97 262 1302.2 0 262 98 292 1302.2 0 292 99 929 1302.2 0 929 100 109 1302.2 0 109 101 121 1302.2 0 121 102 37 1302.2 0 37 103 40 1302.2 0 40 104 97 1302.2 0 97 105 51 1302.2 0 51 106 55 1302.2 0 55 107 72 1302.2 0 72 108 175 1302.2 0 175 109 157 1302.2 0 157 110 77 1302.2 0 77 111 81 1302.2 0 81 112 62 1302.2 0 62 113 152 1302.2 0 152 114 115 1302.2 0 115 115 159 1302.2 0 159 116 220 1302.2 0 220 117 379 1302.2 0 379 118 119 1302.2 0 119 119 160 1302.2 0 160 120 567 1302.2 0 567 121 353 1302.2 0 353 122 423 1302.2 0 423 123 248 1302.2 0 248 124 204 1302.2 0 204 125 250 1302.2 0 250 126 206 1302.2 0 206 127 177 1302.2 0 177 128 246 1302.2 0 246 129 563 1302.2 0 563 130 595 1302.2 0 595 131 329 1302.2 0 329 132 416 1302.2 0 416 133 464 1302.2 0 464 134 701 1302.2 0 701 135 4068 1302.2 0 4068 136 2828 1302.2 0 2828 137 3175 1302.2 0 3175 138 439 1302.2 0 439 139 310 1302.2 0 310 140 353 1302.2 0 353 141 257 1302.2 0 257 142 349 1302.2 0 349 143 433 1302.2 0 433 144 466 1302.2 0 466 145 404 1302.2 0 404 146 301 1302.2 0 301 147 178 1302.2 0 178 148 180 1302.2 0 180 149 169 1302.2 0 169 150 191 1302.2 0 191 151 157 1302.2 0 157 152 128 1302.2 0 128 153 161 1302.2 0 161 154 191 1302.2 0 191 155 364 1302.2 0 364 156 471 1302.2 0 471 157 299 1302.2 0 299 158 312 1302.2 0 312 159 277 1302.2 0 277 160 191 1302.2 0 191 161 249 1302.2 0 249 162 190 1302.2 0 190 163 389 1302.2 0 389 164 309 1302.2 0 309 165 495 1302.2 0 495 166 508 1302.2 0 508 167 595 1302.2 0 595 168 543 1302.2 0 543 169 171 1302.2 0 171 170 159 1302.2 0 159 171 124 1302.2 0 124 172 285 1302.2 0 285 173 264 1302.2 0 264 174 335 1302.2 0 335 175 169 1302.2 0 169 176 108 1302.2 0 108 177 128 1302.2 0 128 178 222 1302.2 0 222 179 371 1302.2 0 371 180 560 1302.2 0 560 181 644 1302.2 0 644 182 466 1302.2 0 466 183 404 1302.2 0 404 184 419 1302.2 0 419 185 320 1302.2 0 320 186 210 1302.2 0 210 187 94 1302.2 0 94 188 144 1302.2 0 144 189 283 1302.2 0 283 190 668 1302.2 0 668 191 647 1302.2 0 647 192 551 1302.2 0 551 193 288 1302.2 0 288 194 296 1302.2 0 296 195 316 1302.2 0 316 196 1206 1302.2 0 1206 197 662 1302.2 0 662 198 157 1302.2 0 157 199 52 1302.2 0 52 200 90 1302.2 0 90 201 661 1302.2 0 661 202 2241 1302.2 0 2241 203 2282 1302.2 0 2282 204 1172 1302.2 0 1172 205 306 1302.2 0 306 206 186 1302.2 0 186 207 305 1302.2 0 305 208 769 1302.2 0 769 209 961 1302.2 0 961 210 924 1302.2 0 924 211 844 1302.2 0 844 212 944 1302.2 0 944 213 796 1302.2 0 796 214 470 1302.2 0 470 215 781 1302.2 0 781 216 953 1302.2 0 953 217 594 1302.2 0 594 218 347 1302.2 0 347 219 210 1302.2 0 210 220 376 1302.2 0 376 221 585 1302.2 0 585 222 1288 1302.2 0 1288 223 499 1302.2 0 499 224 635 1302.2 0 635 225 778 1302.2 0 778 226 629 1302.2 0 629 227 447 1302.2 0 447 228 447 1302.2 0 447 229 237 1302.2 0 237 230 169 1302.2 0 169 231 140 1302.2 0 140 232 282 1302.2 0 282 233 421 1302.2 0 421 234 535 1302.2 0 535 235 830 1302.2 0 830 236 762 1302.2 0 762 237 354 1302.2 0 354 238 163 1302.2 0 163 239 114 1302.2 0 114 240 122 1302.2 0 122 241 278 1302.2 0 278 242 498 1302.2 0 498 243 963 1302.2 0 963 244 808 1302.2 0 808 245 508 1302.2 0 508 246 210 1302.2 0 210 247 98 1302.2 0 98 248 75 1302.2 0 75 249 168 1302.2 0 168 250 318 1302.2 0 318 251 752 1302.2 0 752 252 566 1302.2 0 566 253 629 1302.2 0 629 254 414 1302.2 0 414 255 528 1302.2 0 528 256 483 1302.2 0 483 257 226 1302.2 0 226 258 181 1302.2 0 181 259 240 1302.2 0 240 260 377 1302.2 0 377 261 646 1302.2 0 646 262 634 1302.2 0 634 263 531 1302.2 0 531 264 653 1302.2 0 653 265 581 1302.2 0 581 266 512 1302.2 0 512 267 206 1302.2 0 206 268 89 1302.2 0 89 269 350 1302.2 0 350 270 545 1302.2 0 545 271 1157 1302.2 0 1157 272 758 1302.2 0 758 273 643 1302.2 0 643 274 392 1302.2 0 392 275 474 1302.2 0 474 276 342 1302.2 0 342 277 506 1302.2 0 506 278 547 1302.2 0 547 279 452 1302.2 0 452 280 440 1302.2 0 440 281 513 1302.2 0 513 282 607 1302.2 0 607 283 515 1302.2 0 515 284 817 1302.2 0 817 285 629 1302.2 0 629 286 441 1302.2 0 441 287 203 1302.2 0 203 288 135 1302.2 0 135 289 227 1302.2 0 227 290 406 1302.2 0 406 291 643 1302.2 0 643 292 422 1302.2 0 422 293 218 1302.2 0 218 294 179 1302.2 0 179 295 354 1302.2 0 354 296 553 1302.2 0 553 297 672 1302.2 0 672 298 977 1302.2 0 977 299 970 1302.2 0 970 300 783 1302.2 0 783 301 215 1302.2 0 215 Finished in 2.91 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 67,835 Reads with adapters: 20,637 (30.4%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 20,627 (30.4%) Total basepairs processed: 13,695,561 bp Total written (filtered): 5,712,254 bp (41.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 20637 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 8 0.0 3 0 0 8 16 191 0.0 3 0 189 2 17 20281 0.0 3 19910 360 11 18 68 0.0 3 7 30 28 3 19 40 0.0 3 0 0 21 19 20 21 0.0 3 0 0 0 21 25 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 64 4 0.0 3 0 0 0 4 66 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.94 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 20,627 Reads with adapters: 1,671 (8.1%) Reads written (passing filters): 20,627 (100.0%) Total basepairs processed: 5,712,254 bp Total written (filtered): 5,707,239 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1671 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.0% C: 87.8% G: 5.0% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1670 322.3 0 1670 5 1 20.1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.