This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 174.75 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 114,685 (2.2%) Reads that were too short: 21,332 (0.4%) Reads written (passing filters): 93,353 (1.8%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 22,199,409 bp (1.4%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 114685 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1321 1302.2 0 1321 7 38713 1302.2 0 38713 8 114 1302.2 0 114 9 4426 1302.2 0 4426 10 20 1302.2 0 20 11 18 1302.2 0 18 12 18 1302.2 0 18 13 18 1302.2 0 18 14 17 1302.2 0 17 15 12 1302.2 0 12 16 53 1302.2 0 53 17 81 1302.2 0 81 18 73 1302.2 0 73 19 15 1302.2 0 15 20 28 1302.2 0 28 21 17 1302.2 0 17 22 26 1302.2 0 26 23 15 1302.2 0 15 24 87 1302.2 0 87 25 18 1302.2 0 18 26 12 1302.2 0 12 27 20 1302.2 0 20 28 17 1302.2 0 17 29 21 1302.2 0 21 30 21 1302.2 0 21 31 13 1302.2 0 13 32 16 1302.2 0 16 33 30 1302.2 0 30 34 13 1302.2 0 13 35 43 1302.2 0 43 36 18 1302.2 0 18 37 36 1302.2 0 36 38 21 1302.2 0 21 39 26 1302.2 0 26 40 15 1302.2 0 15 41 23 1302.2 0 23 42 30 1302.2 0 30 43 23 1302.2 0 23 44 16 1302.2 0 16 45 26 1302.2 0 26 46 33 1302.2 0 33 47 40 1302.2 0 40 48 1780 1302.2 0 1780 49 19 1302.2 0 19 50 16 1302.2 0 16 51 18 1302.2 0 18 52 49 1302.2 0 49 53 29 1302.2 0 29 54 19 1302.2 0 19 55 36 1302.2 0 36 56 351 1302.2 0 351 57 220 1302.2 0 220 58 356 1302.2 0 356 59 22 1302.2 0 22 60 10 1302.2 0 10 61 7 1302.2 0 7 62 15 1302.2 0 15 63 28 1302.2 0 28 64 7 1302.2 0 7 65 20 1302.2 0 20 66 21 1302.2 0 21 67 18 1302.2 0 18 68 26 1302.2 0 26 69 11 1302.2 0 11 70 22 1302.2 0 22 71 13 1302.2 0 13 72 21 1302.2 0 21 73 55 1302.2 0 55 74 49 1302.2 0 49 75 25 1302.2 0 25 76 47 1302.2 0 47 77 194 1302.2 0 194 78 327 1302.2 0 327 79 372 1302.2 0 372 80 417 1302.2 0 417 81 1961 1302.2 0 1961 82 1093 1302.2 0 1093 83 388 1302.2 0 388 84 808 1302.2 0 808 85 841 1302.2 0 841 86 751 1302.2 0 751 87 217 1302.2 0 217 88 148 1302.2 0 148 89 176 1302.2 0 176 90 165 1302.2 0 165 91 151 1302.2 0 151 92 221 1302.2 0 221 93 373 1302.2 0 373 94 3512 1302.2 0 3512 95 4410 1302.2 0 4410 96 6265 1302.2 0 6265 97 301 1302.2 0 301 98 208 1302.2 0 208 99 115 1302.2 0 115 100 121 1302.2 0 121 101 163 1302.2 0 163 102 44 1302.2 0 44 103 55 1302.2 0 55 104 39 1302.2 0 39 105 24 1302.2 0 24 106 37 1302.2 0 37 107 44 1302.2 0 44 108 38 1302.2 0 38 109 47 1302.2 0 47 110 361 1302.2 0 361 111 150 1302.2 0 150 112 321 1302.2 0 321 113 79 1302.2 0 79 114 73 1302.2 0 73 115 271 1302.2 0 271 116 199 1302.2 0 199 117 195 1302.2 0 195 118 214 1302.2 0 214 119 232 1302.2 0 232 120 167 1302.2 0 167 121 133 1302.2 0 133 122 109 1302.2 0 109 123 142 1302.2 0 142 124 175 1302.2 0 175 125 154 1302.2 0 154 126 223 1302.2 0 223 127 198 1302.2 0 198 128 185 1302.2 0 185 129 125 1302.2 0 125 130 193 1302.2 0 193 131 372 1302.2 0 372 132 545 1302.2 0 545 133 497 1302.2 0 497 134 187 1302.2 0 187 135 132 1302.2 0 132 136 240 1302.2 0 240 137 503 1302.2 0 503 138 462 1302.2 0 462 139 430 1302.2 0 430 140 324 1302.2 0 324 141 249 1302.2 0 249 142 137 1302.2 0 137 143 148 1302.2 0 148 144 171 1302.2 0 171 145 478 1302.2 0 478 146 373 1302.2 0 373 147 148 1302.2 0 148 148 125 1302.2 0 125 149 91 1302.2 0 91 150 108 1302.2 0 108 151 96 1302.2 0 96 152 120 1302.2 0 120 153 82 1302.2 0 82 154 166 1302.2 0 166 155 171 1302.2 0 171 156 155 1302.2 0 155 157 267 1302.2 0 267 158 263 1302.2 0 263 159 261 1302.2 0 261 160 201 1302.2 0 201 161 201 1302.2 0 201 162 150 1302.2 0 150 163 177 1302.2 0 177 164 185 1302.2 0 185 165 261 1302.2 0 261 166 278 1302.2 0 278 167 238 1302.2 0 238 168 214 1302.2 0 214 169 170 1302.2 0 170 170 263 1302.2 0 263 171 138 1302.2 0 138 172 111 1302.2 0 111 173 84 1302.2 0 84 174 204 1302.2 0 204 175 161 1302.2 0 161 176 187 1302.2 0 187 177 107 1302.2 0 107 178 85 1302.2 0 85 179 154 1302.2 0 154 180 314 1302.2 0 314 181 386 1302.2 0 386 182 256 1302.2 0 256 183 196 1302.2 0 196 184 224 1302.2 0 224 185 192 1302.2 0 192 186 230 1302.2 0 230 187 291 1302.2 0 291 188 133 1302.2 0 133 189 52 1302.2 0 52 190 133 1302.2 0 133 191 266 1302.2 0 266 192 536 1302.2 0 536 193 347 1302.2 0 347 194 1046 1302.2 0 1046 195 191 1302.2 0 191 196 109 1302.2 0 109 197 108 1302.2 0 108 198 181 1302.2 0 181 199 219 1302.2 0 219 200 179 1302.2 0 179 201 56 1302.2 0 56 202 57 1302.2 0 57 203 200 1302.2 0 200 204 446 1302.2 0 446 205 549 1302.2 0 549 206 446 1302.2 0 446 207 324 1302.2 0 324 208 134 1302.2 0 134 209 119 1302.2 0 119 210 183 1302.2 0 183 211 338 1302.2 0 338 212 324 1302.2 0 324 213 348 1302.2 0 348 214 289 1302.2 0 289 215 234 1302.2 0 234 216 215 1302.2 0 215 217 238 1302.2 0 238 218 375 1302.2 0 375 219 314 1302.2 0 314 220 243 1302.2 0 243 221 116 1302.2 0 116 222 84 1302.2 0 84 223 195 1302.2 0 195 224 267 1302.2 0 267 225 238 1302.2 0 238 226 241 1302.2 0 241 227 298 1302.2 0 298 228 294 1302.2 0 294 229 246 1302.2 0 246 230 147 1302.2 0 147 231 160 1302.2 0 160 232 152 1302.2 0 152 233 91 1302.2 0 91 234 173 1302.2 0 173 235 265 1302.2 0 265 236 280 1302.2 0 280 237 164 1302.2 0 164 238 224 1302.2 0 224 239 239 1302.2 0 239 240 192 1302.2 0 192 241 82 1302.2 0 82 242 87 1302.2 0 87 243 143 1302.2 0 143 244 243 1302.2 0 243 245 233 1302.2 0 233 246 154 1302.2 0 154 247 163 1302.2 0 163 248 104 1302.2 0 104 249 108 1302.2 0 108 250 91 1302.2 0 91 251 93 1302.2 0 93 252 130 1302.2 0 130 253 177 1302.2 0 177 254 158 1302.2 0 158 255 136 1302.2 0 136 256 144 1302.2 0 144 257 181 1302.2 0 181 258 131 1302.2 0 131 259 131 1302.2 0 131 260 83 1302.2 0 83 261 113 1302.2 0 113 262 221 1302.2 0 221 263 157 1302.2 0 157 264 150 1302.2 0 150 265 230 1302.2 0 230 266 264 1302.2 0 264 267 313 1302.2 0 313 268 332 1302.2 0 332 269 115 1302.2 0 115 270 67 1302.2 0 67 271 187 1302.2 0 187 272 533 1302.2 0 533 273 281 1302.2 0 281 274 199 1302.2 0 199 275 157 1302.2 0 157 276 151 1302.2 0 151 277 91 1302.2 0 91 278 161 1302.2 0 161 279 296 1302.2 0 296 280 194 1302.2 0 194 281 106 1302.2 0 106 282 96 1302.2 0 96 283 105 1302.2 0 105 284 142 1302.2 0 142 285 176 1302.2 0 176 286 236 1302.2 0 236 287 257 1302.2 0 257 288 329 1302.2 0 329 289 260 1302.2 0 260 290 251 1302.2 0 251 291 308 1302.2 0 308 292 338 1302.2 0 338 293 268 1302.2 0 268 294 197 1302.2 0 197 295 158 1302.2 0 158 296 109 1302.2 0 109 297 337 1302.2 0 337 298 564 1302.2 0 564 299 252 1302.2 0 252 300 110 1302.2 0 110 301 307 1302.2 0 307 Finished in 4.77 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 93,353 Reads with adapters: 39,417 (42.2%) Reads that were too short: 43 (0.0%) Reads written (passing filters): 39,374 (42.2%) Total basepairs processed: 22,199,409 bp Total written (filtered): 10,786,189 bp (48.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 39417 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 1 1 18 11 0.0 3 0 0 10 1 19 386 0.0 3 0 359 17 8 2 20 38823 0.0 4 37833 862 90 28 10 21 84 0.0 4 4 73 7 22 5 0.0 4 1 0 4 23 34 0.0 4 0 0 0 29 5 24 2 0.0 4 0 0 0 0 2 25 1 0.0 4 0 1 35 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 48 5 0.0 4 0 0 0 0 5 49 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 72 1 0.0 4 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 1 88 1 0.0 4 0 0 0 0 1 93 5 0.0 4 0 0 0 0 5 98 1 0.0 4 0 0 0 0 1 102 2 0.0 4 0 0 0 0 2 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 130 5 0.0 4 0 0 0 0 5 131 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 141 25 0.0 4 0 0 0 1 24 142 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 163 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 1 216 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.67 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 39,374 Reads with adapters: 1,810 (4.6%) Reads written (passing filters): 39,374 (100.0%) Total basepairs processed: 10,786,189 bp Total written (filtered): 10,764,529 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1810 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 3.4% G: 92.1% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 615.2 0 11 4 5 153.8 0 5 9 2 0.2 0 0 2 10 3 0.0 1 0 3 11 1688 0.0 1 7 1681 12 86 0.0 1 5 81 13 4 0.0 1 0 4 14 1 0.0 1 0 1 15 1 0.0 1 0 1 152 2 0.0 1 1 1 209 1 0.0 1 0 1 210 4 0.0 1 4 259 1 0.0 1 1 266 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.