This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 168.11 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 155,248 (2.9%) Reads that were too short: 15,176 (0.3%) Reads written (passing filters): 140,072 (2.6%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 25,504,761 bp (1.6%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 155248 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 219 1302.2 0 219 7 923 1302.2 0 923 8 208 1302.2 0 208 9 6492 1302.2 0 6492 10 35 1302.2 0 35 11 28 1302.2 0 28 12 16 1302.2 0 16 13 11 1302.2 0 11 14 86 1302.2 0 86 15 40 1302.2 0 40 16 13 1302.2 0 13 17 6 1302.2 0 6 18 10 1302.2 0 10 19 15 1302.2 0 15 20 14 1302.2 0 14 21 15 1302.2 0 15 22 9 1302.2 0 9 23 16 1302.2 0 16 24 32 1302.2 0 32 25 11 1302.2 0 11 26 11 1302.2 0 11 27 14 1302.2 0 14 28 9 1302.2 0 9 29 18 1302.2 0 18 30 13 1302.2 0 13 31 12 1302.2 0 12 32 11 1302.2 0 11 33 26 1302.2 0 26 34 25 1302.2 0 25 35 13 1302.2 0 13 36 19 1302.2 0 19 37 71 1302.2 0 71 38 12 1302.2 0 12 39 26 1302.2 0 26 40 51 1302.2 0 51 41 108 1302.2 0 108 42 14 1302.2 0 14 43 58 1302.2 0 58 44 17 1302.2 0 17 45 39 1302.2 0 39 46 16 1302.2 0 16 47 12 1302.2 0 12 48 19 1302.2 0 19 49 15 1302.2 0 15 50 17 1302.2 0 17 51 15 1302.2 0 15 52 25 1302.2 0 25 53 13 1302.2 0 13 54 25 1302.2 0 25 55 13 1302.2 0 13 56 37 1302.2 0 37 57 28 1302.2 0 28 58 84 1302.2 0 84 59 53 1302.2 0 53 60 13 1302.2 0 13 61 34 1302.2 0 34 62 11 1302.2 0 11 63 21 1302.2 0 21 64 10 1302.2 0 10 65 16 1302.2 0 16 66 6 1302.2 0 6 67 9 1302.2 0 9 68 14 1302.2 0 14 69 10 1302.2 0 10 70 23 1302.2 0 23 71 12 1302.2 0 12 72 10 1302.2 0 10 73 8 1302.2 0 8 74 19 1302.2 0 19 75 20 1302.2 0 20 76 20 1302.2 0 20 77 132 1302.2 0 132 78 34 1302.2 0 34 79 46 1302.2 0 46 80 49 1302.2 0 49 81 124 1302.2 0 124 82 61 1302.2 0 61 83 519 1302.2 0 519 84 275 1302.2 0 275 85 246 1302.2 0 246 86 52 1302.2 0 52 87 165 1302.2 0 165 88 52 1302.2 0 52 89 91 1302.2 0 91 90 121 1302.2 0 121 91 187 1302.2 0 187 92 223 1302.2 0 223 93 198 1302.2 0 198 94 403 1302.2 0 403 95 58 1302.2 0 58 96 128 1302.2 0 128 97 437 1302.2 0 437 98 90 1302.2 0 90 99 149 1302.2 0 149 100 82 1302.2 0 82 101 50 1302.2 0 50 102 178 1302.2 0 178 103 97 1302.2 0 97 104 118 1302.2 0 118 105 97 1302.2 0 97 106 130 1302.2 0 130 107 318 1302.2 0 318 108 522 1302.2 0 522 109 426 1302.2 0 426 110 694 1302.2 0 694 111 733 1302.2 0 733 112 1178 1302.2 0 1178 113 2139 1302.2 0 2139 114 33909 1302.2 0 33909 115 5752 1302.2 0 5752 116 37044 1302.2 0 37044 117 310 1302.2 0 310 118 115 1302.2 0 115 119 59 1302.2 0 59 120 55 1302.2 0 55 121 46 1302.2 0 46 122 126 1302.2 0 126 123 82 1302.2 0 82 124 94 1302.2 0 94 125 72 1302.2 0 72 126 132 1302.2 0 132 127 64 1302.2 0 64 128 458 1302.2 0 458 129 333 1302.2 0 333 130 183 1302.2 0 183 131 217 1302.2 0 217 132 79 1302.2 0 79 133 114 1302.2 0 114 134 83 1302.2 0 83 135 182 1302.2 0 182 136 316 1302.2 0 316 137 261 1302.2 0 261 138 272 1302.2 0 272 139 146 1302.2 0 146 140 455 1302.2 0 455 141 1060 1302.2 0 1060 142 1290 1302.2 0 1290 143 339 1302.2 0 339 144 216 1302.2 0 216 145 363 1302.2 0 363 146 429 1302.2 0 429 147 7147 1302.2 0 7147 148 992 1302.2 0 992 149 5007 1302.2 0 5007 150 305 1302.2 0 305 151 281 1302.2 0 281 152 468 1302.2 0 468 153 6430 1302.2 0 6430 154 1359 1302.2 0 1359 155 4074 1302.2 0 4074 156 906 1302.2 0 906 157 792 1302.2 0 792 158 313 1302.2 0 313 159 93 1302.2 0 93 160 173 1302.2 0 173 161 194 1302.2 0 194 162 282 1302.2 0 282 163 216 1302.2 0 216 164 238 1302.2 0 238 165 276 1302.2 0 276 166 194 1302.2 0 194 167 258 1302.2 0 258 168 812 1302.2 0 812 169 522 1302.2 0 522 170 362 1302.2 0 362 171 154 1302.2 0 154 172 92 1302.2 0 92 173 55 1302.2 0 55 174 69 1302.2 0 69 175 131 1302.2 0 131 176 179 1302.2 0 179 177 60 1302.2 0 60 178 88 1302.2 0 88 179 92 1302.2 0 92 180 108 1302.2 0 108 181 300 1302.2 0 300 182 255 1302.2 0 255 183 315 1302.2 0 315 184 245 1302.2 0 245 185 275 1302.2 0 275 186 172 1302.2 0 172 187 192 1302.2 0 192 188 52 1302.2 0 52 189 45 1302.2 0 45 190 57 1302.2 0 57 191 96 1302.2 0 96 192 132 1302.2 0 132 193 104 1302.2 0 104 194 83 1302.2 0 83 195 38 1302.2 0 38 196 56 1302.2 0 56 197 80 1302.2 0 80 198 52 1302.2 0 52 199 45 1302.2 0 45 200 44 1302.2 0 44 201 60 1302.2 0 60 202 447 1302.2 0 447 203 188 1302.2 0 188 204 468 1302.2 0 468 205 217 1302.2 0 217 206 110 1302.2 0 110 207 73 1302.2 0 73 208 46 1302.2 0 46 209 58 1302.2 0 58 210 123 1302.2 0 123 211 86 1302.2 0 86 212 161 1302.2 0 161 213 172 1302.2 0 172 214 149 1302.2 0 149 215 145 1302.2 0 145 216 343 1302.2 0 343 217 240 1302.2 0 240 218 257 1302.2 0 257 219 112 1302.2 0 112 220 44 1302.2 0 44 221 61 1302.2 0 61 222 89 1302.2 0 89 223 57 1302.2 0 57 224 180 1302.2 0 180 225 247 1302.2 0 247 226 394 1302.2 0 394 227 315 1302.2 0 315 228 307 1302.2 0 307 229 157 1302.2 0 157 230 74 1302.2 0 74 231 51 1302.2 0 51 232 80 1302.2 0 80 233 94 1302.2 0 94 234 56 1302.2 0 56 235 135 1302.2 0 135 236 127 1302.2 0 127 237 93 1302.2 0 93 238 47 1302.2 0 47 239 27 1302.2 0 27 240 33 1302.2 0 33 241 30 1302.2 0 30 242 48 1302.2 0 48 243 48 1302.2 0 48 244 115 1302.2 0 115 245 203 1302.2 0 203 246 146 1302.2 0 146 247 112 1302.2 0 112 248 62 1302.2 0 62 249 55 1302.2 0 55 250 54 1302.2 0 54 251 149 1302.2 0 149 252 154 1302.2 0 154 253 184 1302.2 0 184 254 363 1302.2 0 363 255 394 1302.2 0 394 256 288 1302.2 0 288 257 99 1302.2 0 99 258 59 1302.2 0 59 259 43 1302.2 0 43 260 20 1302.2 0 20 261 23 1302.2 0 23 262 24 1302.2 0 24 263 38 1302.2 0 38 264 78 1302.2 0 78 265 94 1302.2 0 94 266 224 1302.2 0 224 267 173 1302.2 0 173 268 120 1302.2 0 120 269 76 1302.2 0 76 270 93 1302.2 0 93 271 119 1302.2 0 119 272 93 1302.2 0 93 273 118 1302.2 0 118 274 202 1302.2 0 202 275 353 1302.2 0 353 276 116 1302.2 0 116 277 84 1302.2 0 84 278 104 1302.2 0 104 279 148 1302.2 0 148 280 248 1302.2 0 248 281 413 1302.2 0 413 282 477 1302.2 0 477 283 352 1302.2 0 352 284 80 1302.2 0 80 285 88 1302.2 0 88 286 41 1302.2 0 41 287 112 1302.2 0 112 288 45 1302.2 0 45 289 238 1302.2 0 238 290 445 1302.2 0 445 291 442 1302.2 0 442 292 322 1302.2 0 322 293 284 1302.2 0 284 294 156 1302.2 0 156 295 138 1302.2 0 138 296 142 1302.2 0 142 297 29 1302.2 0 29 298 30 1302.2 0 30 299 62 1302.2 0 62 300 31 1302.2 0 31 301 32 1302.2 0 32 Finished in 6.52 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 140,072 Reads with adapters: 6,683 (4.8%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 6,671 (4.8%) Total basepairs processed: 25,504,761 bp Total written (filtered): 1,812,874 bp (7.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6683 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 18 7 0.0 3 0 0 5 1 1 19 114 0.0 3 0 97 2 1 14 20 6431 0.0 4 6224 154 20 18 15 21 71 0.0 4 33 14 1 17 6 22 26 0.0 4 1 0 0 0 25 23 9 0.0 4 0 1 0 6 2 25 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 43 2 0.0 4 0 0 0 0 2 48 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 91 1 0.0 4 1 105 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 6,671 Reads with adapters: 1,533 (23.0%) Reads written (passing filters): 6,671 (100.0%) Total basepairs processed: 1,812,874 bp Total written (filtered): 1,467,279 bp (80.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1533 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 0.3% G: 15.3% T: 83.2% none/other: 0.1% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "T" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 8 1 0.1 0 1 9 42 0.0 0 1 41 10 99 0.0 1 1 98 11 104 0.0 1 0 104 13 1 0.0 1 0 1 83 2 0.0 1 1 1 257 5 0.0 1 4 1 262 1 0.0 1 1 266 9 0.0 1 7 2 267 1192 0.0 1 1132 60 268 42 0.0 1 42 269 19 0.0 1 19 270 13 0.0 1 13 271 2 0.0 1 2 272 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.