This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 170.20 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 144,037 (2.7%) Reads that were too short: 53,294 (1.0%) Reads written (passing filters): 90,743 (1.7%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 19,909,553 bp (1.2%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 144037 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 175 1302.2 0 175 7 308 1302.2 0 308 8 520 1302.2 0 520 9 27653 1302.2 0 27653 10 65 1302.2 0 65 11 30 1302.2 0 30 12 28 1302.2 0 28 13 32 1302.2 0 32 14 28 1302.2 0 28 15 37 1302.2 0 37 16 42 1302.2 0 42 17 28 1302.2 0 28 18 28 1302.2 0 28 19 23 1302.2 0 23 20 25 1302.2 0 25 21 42 1302.2 0 42 22 34 1302.2 0 34 23 35 1302.2 0 35 24 33 1302.2 0 33 25 45 1302.2 0 45 26 59 1302.2 0 59 27 30 1302.2 0 30 28 33 1302.2 0 33 29 56 1302.2 0 56 30 42 1302.2 0 42 31 50 1302.2 0 50 32 94 1302.2 0 94 33 54 1302.2 0 54 34 41 1302.2 0 41 35 257 1302.2 0 257 36 185 1302.2 0 185 37 467 1302.2 0 467 38 66 1302.2 0 66 39 58 1302.2 0 58 40 68 1302.2 0 68 41 121 1302.2 0 121 42 241 1302.2 0 241 43 6611 1302.2 0 6611 44 66 1302.2 0 66 45 30 1302.2 0 30 46 24 1302.2 0 24 47 25 1302.2 0 25 48 19 1302.2 0 19 49 26 1302.2 0 26 50 31 1302.2 0 31 51 30 1302.2 0 30 52 53 1302.2 0 53 53 36 1302.2 0 36 54 46 1302.2 0 46 55 22 1302.2 0 22 56 28 1302.2 0 28 57 21 1302.2 0 21 58 26 1302.2 0 26 59 32 1302.2 0 32 60 37 1302.2 0 37 61 29 1302.2 0 29 62 36 1302.2 0 36 63 28 1302.2 0 28 64 42 1302.2 0 42 65 36 1302.2 0 36 66 26 1302.2 0 26 67 48 1302.2 0 48 68 62 1302.2 0 62 69 270 1302.2 0 270 70 113 1302.2 0 113 71 243 1302.2 0 243 72 33 1302.2 0 33 73 23 1302.2 0 23 74 30 1302.2 0 30 75 27 1302.2 0 27 76 18 1302.2 0 18 77 36 1302.2 0 36 78 32 1302.2 0 32 79 53 1302.2 0 53 80 100 1302.2 0 100 81 169 1302.2 0 169 82 236 1302.2 0 236 83 95 1302.2 0 95 84 99 1302.2 0 99 85 57 1302.2 0 57 86 50 1302.2 0 50 87 110 1302.2 0 110 88 283 1302.2 0 283 89 156 1302.2 0 156 90 981 1302.2 0 981 91 69 1302.2 0 69 92 425 1302.2 0 425 93 148 1302.2 0 148 94 212 1302.2 0 212 95 112 1302.2 0 112 96 316 1302.2 0 316 97 1402 1302.2 0 1402 98 3272 1302.2 0 3272 99 7017 1302.2 0 7017 100 2907 1302.2 0 2907 101 1215 1302.2 0 1215 102 250 1302.2 0 250 103 211 1302.2 0 211 104 885 1302.2 0 885 105 270 1302.2 0 270 106 203 1302.2 0 203 107 326 1302.2 0 326 108 196 1302.2 0 196 109 359 1302.2 0 359 110 577 1302.2 0 577 111 722 1302.2 0 722 112 1353 1302.2 0 1353 113 274 1302.2 0 274 114 130 1302.2 0 130 115 200 1302.2 0 200 116 169 1302.2 0 169 117 81 1302.2 0 81 118 254 1302.2 0 254 119 161 1302.2 0 161 120 210 1302.2 0 210 121 410 1302.2 0 410 122 359 1302.2 0 359 123 273 1302.2 0 273 124 290 1302.2 0 290 125 237 1302.2 0 237 126 148 1302.2 0 148 127 272 1302.2 0 272 128 287 1302.2 0 287 129 412 1302.2 0 412 130 316 1302.2 0 316 131 480 1302.2 0 480 132 518 1302.2 0 518 133 644 1302.2 0 644 134 412 1302.2 0 412 135 291 1302.2 0 291 136 241 1302.2 0 241 137 249 1302.2 0 249 138 222 1302.2 0 222 139 596 1302.2 0 596 140 340 1302.2 0 340 141 382 1302.2 0 382 142 328 1302.2 0 328 143 322 1302.2 0 322 144 252 1302.2 0 252 145 151 1302.2 0 151 146 191 1302.2 0 191 147 258 1302.2 0 258 148 345 1302.2 0 345 149 256 1302.2 0 256 150 83 1302.2 0 83 151 114 1302.2 0 114 152 118 1302.2 0 118 153 134 1302.2 0 134 154 218 1302.2 0 218 155 478 1302.2 0 478 156 239 1302.2 0 239 157 334 1302.2 0 334 158 234 1302.2 0 234 159 533 1302.2 0 533 160 271 1302.2 0 271 161 295 1302.2 0 295 162 247 1302.2 0 247 163 218 1302.2 0 218 164 355 1302.2 0 355 165 248 1302.2 0 248 166 279 1302.2 0 279 167 385 1302.2 0 385 168 697 1302.2 0 697 169 229 1302.2 0 229 170 286 1302.2 0 286 171 291 1302.2 0 291 172 277 1302.2 0 277 173 337 1302.2 0 337 174 200 1302.2 0 200 175 185 1302.2 0 185 176 155 1302.2 0 155 177 242 1302.2 0 242 178 226 1302.2 0 226 179 233 1302.2 0 233 180 230 1302.2 0 230 181 185 1302.2 0 185 182 308 1302.2 0 308 183 323 1302.2 0 323 184 343 1302.2 0 343 185 267 1302.2 0 267 186 152 1302.2 0 152 187 182 1302.2 0 182 188 220 1302.2 0 220 189 341 1302.2 0 341 190 288 1302.2 0 288 191 357 1302.2 0 357 192 339 1302.2 0 339 193 452 1302.2 0 452 194 280 1302.2 0 280 195 888 1302.2 0 888 196 359 1302.2 0 359 197 450 1302.2 0 450 198 168 1302.2 0 168 199 146 1302.2 0 146 200 427 1302.2 0 427 201 672 1302.2 0 672 202 826 1302.2 0 826 203 656 1302.2 0 656 204 310 1302.2 0 310 205 371 1302.2 0 371 206 959 1302.2 0 959 207 1913 1302.2 0 1913 208 1384 1302.2 0 1384 209 994 1302.2 0 994 210 421 1302.2 0 421 211 506 1302.2 0 506 212 515 1302.2 0 515 213 372 1302.2 0 372 214 456 1302.2 0 456 215 455 1302.2 0 455 216 391 1302.2 0 391 217 329 1302.2 0 329 218 279 1302.2 0 279 219 228 1302.2 0 228 220 426 1302.2 0 426 221 684 1302.2 0 684 222 487 1302.2 0 487 223 654 1302.2 0 654 224 341 1302.2 0 341 225 408 1302.2 0 408 226 373 1302.2 0 373 227 378 1302.2 0 378 228 304 1302.2 0 304 229 431 1302.2 0 431 230 382 1302.2 0 382 231 433 1302.2 0 433 232 501 1302.2 0 501 233 489 1302.2 0 489 234 449 1302.2 0 449 235 956 1302.2 0 956 236 402 1302.2 0 402 237 894 1302.2 0 894 238 395 1302.2 0 395 239 544 1302.2 0 544 240 734 1302.2 0 734 241 2627 1302.2 0 2627 242 1452 1302.2 0 1452 243 873 1302.2 0 873 244 333 1302.2 0 333 245 277 1302.2 0 277 246 228 1302.2 0 228 247 132 1302.2 0 132 248 250 1302.2 0 250 249 588 1302.2 0 588 250 1014 1302.2 0 1014 251 356 1302.2 0 356 252 252 1302.2 0 252 253 159 1302.2 0 159 254 278 1302.2 0 278 255 309 1302.2 0 309 256 185 1302.2 0 185 257 291 1302.2 0 291 258 404 1302.2 0 404 259 679 1302.2 0 679 260 1988 1302.2 0 1988 261 2587 1302.2 0 2587 262 1181 1302.2 0 1181 263 464 1302.2 0 464 264 304 1302.2 0 304 265 372 1302.2 0 372 266 202 1302.2 0 202 267 124 1302.2 0 124 268 207 1302.2 0 207 269 362 1302.2 0 362 270 629 1302.2 0 629 271 391 1302.2 0 391 272 418 1302.2 0 418 273 520 1302.2 0 520 274 594 1302.2 0 594 275 846 1302.2 0 846 276 320 1302.2 0 320 277 272 1302.2 0 272 278 286 1302.2 0 286 279 201 1302.2 0 201 280 328 1302.2 0 328 281 516 1302.2 0 516 282 611 1302.2 0 611 283 617 1302.2 0 617 284 272 1302.2 0 272 285 220 1302.2 0 220 286 154 1302.2 0 154 287 149 1302.2 0 149 288 244 1302.2 0 244 289 630 1302.2 0 630 290 520 1302.2 0 520 291 355 1302.2 0 355 292 305 1302.2 0 305 293 146 1302.2 0 146 294 224 1302.2 0 224 295 274 1302.2 0 274 296 322 1302.2 0 322 297 361 1302.2 0 361 298 601 1302.2 0 601 299 427 1302.2 0 427 300 572 1302.2 0 572 301 461 1302.2 0 461 Finished in 4.00 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 90,743 Reads with adapters: 28,427 (31.3%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 28,416 (31.3%) Total basepairs processed: 19,909,553 bp Total written (filtered): 7,812,829 bp (39.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 28427 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 12 0.0 3 0 0 12 16 228 0.0 3 0 221 5 2 17 28029 0.0 3 27401 607 20 1 18 83 0.0 3 5 58 15 5 19 31 0.0 3 0 0 16 15 20 8 0.0 3 0 0 0 8 22 1 0.0 3 1 27 1 0.0 3 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 38 1 0.0 3 1 47 2 0.0 3 0 0 0 2 52 2 0.0 3 1 1 81 3 0.0 3 0 0 0 3 84 6 0.0 3 0 0 0 6 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.26 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 28,416 Reads with adapters: 780 (2.7%) Reads written (passing filters): 28,416 (100.0%) Total basepairs processed: 7,812,829 bp Total written (filtered): 7,810,479 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 780 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.9% C: 23.1% G: 65.1% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 770 444.0 0 770 4 10 111.0 0 10