This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 161.31 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 94,919 (1.8%) Reads that were too short: 19,269 (0.4%) Reads written (passing filters): 75,650 (1.4%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 14,938,985 bp (0.9%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 94919 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 40 1302.2 0 40 7 90 1302.2 0 90 8 3707 1302.2 0 3707 9 55 1302.2 0 55 10 29 1302.2 0 29 11 11 1302.2 0 11 12 13 1302.2 0 13 13 20 1302.2 0 20 14 19 1302.2 0 19 15 14 1302.2 0 14 16 35 1302.2 0 35 17 20 1302.2 0 20 18 13 1302.2 0 13 19 23 1302.2 0 23 20 16 1302.2 0 16 21 15 1302.2 0 15 22 11 1302.2 0 11 23 18 1302.2 0 18 24 16 1302.2 0 16 25 16 1302.2 0 16 26 16 1302.2 0 16 27 11 1302.2 0 11 28 17 1302.2 0 17 29 19 1302.2 0 19 30 39 1302.2 0 39 31 15 1302.2 0 15 32 16 1302.2 0 16 33 33 1302.2 0 33 34 16 1302.2 0 16 35 17 1302.2 0 17 36 28 1302.2 0 28 37 17 1302.2 0 17 38 7 1302.2 0 7 39 20 1302.2 0 20 40 71 1302.2 0 71 41 12 1302.2 0 12 42 34 1302.2 0 34 43 28 1302.2 0 28 44 13 1302.2 0 13 45 19 1302.2 0 19 46 21 1302.2 0 21 47 15 1302.2 0 15 48 19 1302.2 0 19 49 23 1302.2 0 23 50 17 1302.2 0 17 51 19 1302.2 0 19 52 12 1302.2 0 12 53 20 1302.2 0 20 54 67 1302.2 0 67 55 23 1302.2 0 23 56 19 1302.2 0 19 57 18 1302.2 0 18 58 14 1302.2 0 14 59 16 1302.2 0 16 60 27 1302.2 0 27 61 33 1302.2 0 33 62 27 1302.2 0 27 63 41 1302.2 0 41 64 125 1302.2 0 125 65 28 1302.2 0 28 66 56 1302.2 0 56 67 26 1302.2 0 26 68 14 1302.2 0 14 69 43 1302.2 0 43 70 40 1302.2 0 40 71 30 1302.2 0 30 72 43 1302.2 0 43 73 34 1302.2 0 34 74 58 1302.2 0 58 75 93 1302.2 0 93 76 127 1302.2 0 127 77 246 1302.2 0 246 78 247 1302.2 0 247 79 468 1302.2 0 468 80 1214 1302.2 0 1214 81 17406 1302.2 0 17406 82 5328 1302.2 0 5328 83 9062 1302.2 0 9062 84 106 1302.2 0 106 85 119 1302.2 0 119 86 89 1302.2 0 89 87 93 1302.2 0 93 88 13 1302.2 0 13 89 47 1302.2 0 47 90 13 1302.2 0 13 91 17 1302.2 0 17 92 87 1302.2 0 87 93 113 1302.2 0 113 94 27 1302.2 0 27 95 18 1302.2 0 18 96 32 1302.2 0 32 97 49 1302.2 0 49 98 68 1302.2 0 68 99 312 1302.2 0 312 100 71 1302.2 0 71 101 46 1302.2 0 46 102 84 1302.2 0 84 103 116 1302.2 0 116 104 101 1302.2 0 101 105 52 1302.2 0 52 106 51 1302.2 0 51 107 32 1302.2 0 32 108 69 1302.2 0 69 109 201 1302.2 0 201 110 57 1302.2 0 57 111 69 1302.2 0 69 112 107 1302.2 0 107 113 54 1302.2 0 54 114 64 1302.2 0 64 115 103 1302.2 0 103 116 107 1302.2 0 107 117 132 1302.2 0 132 118 270 1302.2 0 270 119 185 1302.2 0 185 120 330 1302.2 0 330 121 474 1302.2 0 474 122 2248 1302.2 0 2248 123 6756 1302.2 0 6756 124 9511 1302.2 0 9511 125 171 1302.2 0 171 126 223 1302.2 0 223 127 74 1302.2 0 74 128 62 1302.2 0 62 129 101 1302.2 0 101 130 199 1302.2 0 199 131 115 1302.2 0 115 132 83 1302.2 0 83 133 151 1302.2 0 151 134 111 1302.2 0 111 135 159 1302.2 0 159 136 139 1302.2 0 139 137 165 1302.2 0 165 138 171 1302.2 0 171 139 154 1302.2 0 154 140 59 1302.2 0 59 141 99 1302.2 0 99 142 101 1302.2 0 101 143 181 1302.2 0 181 144 491 1302.2 0 491 145 148 1302.2 0 148 146 532 1302.2 0 532 147 135 1302.2 0 135 148 73 1302.2 0 73 149 48 1302.2 0 48 150 49 1302.2 0 49 151 135 1302.2 0 135 152 120 1302.2 0 120 153 220 1302.2 0 220 154 125 1302.2 0 125 155 161 1302.2 0 161 156 172 1302.2 0 172 157 170 1302.2 0 170 158 139 1302.2 0 139 159 238 1302.2 0 238 160 134 1302.2 0 134 161 136 1302.2 0 136 162 131 1302.2 0 131 163 189 1302.2 0 189 164 150 1302.2 0 150 165 140 1302.2 0 140 166 114 1302.2 0 114 167 125 1302.2 0 125 168 164 1302.2 0 164 169 96 1302.2 0 96 170 125 1302.2 0 125 171 167 1302.2 0 167 172 293 1302.2 0 293 173 189 1302.2 0 189 174 86 1302.2 0 86 175 84 1302.2 0 84 176 94 1302.2 0 94 177 176 1302.2 0 176 178 256 1302.2 0 256 179 195 1302.2 0 195 180 123 1302.2 0 123 181 136 1302.2 0 136 182 763 1302.2 0 763 183 266 1302.2 0 266 184 695 1302.2 0 695 185 152 1302.2 0 152 186 96 1302.2 0 96 187 57 1302.2 0 57 188 125 1302.2 0 125 189 282 1302.2 0 282 190 562 1302.2 0 562 191 114 1302.2 0 114 192 89 1302.2 0 89 193 67 1302.2 0 67 194 118 1302.2 0 118 195 175 1302.2 0 175 196 336 1302.2 0 336 197 386 1302.2 0 386 198 103 1302.2 0 103 199 73 1302.2 0 73 200 47 1302.2 0 47 201 126 1302.2 0 126 202 173 1302.2 0 173 203 133 1302.2 0 133 204 99 1302.2 0 99 205 195 1302.2 0 195 206 148 1302.2 0 148 207 141 1302.2 0 141 208 96 1302.2 0 96 209 140 1302.2 0 140 210 173 1302.2 0 173 211 215 1302.2 0 215 212 150 1302.2 0 150 213 146 1302.2 0 146 214 167 1302.2 0 167 215 193 1302.2 0 193 216 191 1302.2 0 191 217 168 1302.2 0 168 218 115 1302.2 0 115 219 78 1302.2 0 78 220 155 1302.2 0 155 221 224 1302.2 0 224 222 443 1302.2 0 443 223 238 1302.2 0 238 224 123 1302.2 0 123 225 182 1302.2 0 182 226 150 1302.2 0 150 227 133 1302.2 0 133 228 118 1302.2 0 118 229 85 1302.2 0 85 230 67 1302.2 0 67 231 62 1302.2 0 62 232 88 1302.2 0 88 233 215 1302.2 0 215 234 238 1302.2 0 238 235 91 1302.2 0 91 236 87 1302.2 0 87 237 88 1302.2 0 88 238 101 1302.2 0 101 239 86 1302.2 0 86 240 381 1302.2 0 381 241 178 1302.2 0 178 242 450 1302.2 0 450 243 172 1302.2 0 172 244 161 1302.2 0 161 245 144 1302.2 0 144 246 83 1302.2 0 83 247 50 1302.2 0 50 248 32 1302.2 0 32 249 99 1302.2 0 99 250 147 1302.2 0 147 251 244 1302.2 0 244 252 173 1302.2 0 173 253 167 1302.2 0 167 254 207 1302.2 0 207 255 150 1302.2 0 150 256 107 1302.2 0 107 257 83 1302.2 0 83 258 60 1302.2 0 60 259 134 1302.2 0 134 260 148 1302.2 0 148 261 246 1302.2 0 246 262 414 1302.2 0 414 263 323 1302.2 0 323 264 247 1302.2 0 247 265 203 1302.2 0 203 266 176 1302.2 0 176 267 119 1302.2 0 119 268 87 1302.2 0 87 269 126 1302.2 0 126 270 134 1302.2 0 134 271 141 1302.2 0 141 272 169 1302.2 0 169 273 136 1302.2 0 136 274 124 1302.2 0 124 275 127 1302.2 0 127 276 216 1302.2 0 216 277 230 1302.2 0 230 278 192 1302.2 0 192 279 83 1302.2 0 83 280 107 1302.2 0 107 281 87 1302.2 0 87 282 177 1302.2 0 177 283 263 1302.2 0 263 284 133 1302.2 0 133 285 123 1302.2 0 123 286 75 1302.2 0 75 287 55 1302.2 0 55 288 93 1302.2 0 93 289 197 1302.2 0 197 290 581 1302.2 0 581 291 1870 1302.2 0 1870 292 672 1302.2 0 672 293 221 1302.2 0 221 294 170 1302.2 0 170 295 224 1302.2 0 224 296 524 1302.2 0 524 297 239 1302.2 0 239 298 257 1302.2 0 257 299 311 1302.2 0 311 300 246 1302.2 0 246 301 86 1302.2 0 86 Finished in 3.13 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 75,650 Reads with adapters: 3,862 (5.1%) Reads that were too short: 75 (0.1%) Reads written (passing filters): 3,787 (5.0%) Total basepairs processed: 14,938,985 bp Total written (filtered): 1,038,723 bp (7.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3862 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 16 17 0.0 3 0 14 2 1 17 3630 0.0 3 3579 50 0 1 18 48 0.0 3 1 8 35 4 19 45 0.0 3 2 0 7 36 20 3 0.0 3 0 0 0 3 34 3 0.0 3 0 0 0 3 49 2 0.0 3 0 0 0 2 50 16 0.0 3 0 0 0 16 51 2 0.0 3 0 0 0 2 52 2 0.0 3 0 0 0 2 53 9 0.0 3 0 0 0 9 54 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 161 3 0.0 3 0 0 0 3 162 9 0.0 3 0 0 0 9 163 5 0.0 3 0 0 0 5 164 8 0.0 3 0 0 0 8 165 42 0.0 3 0 0 0 42 166 4 0.0 3 0 0 0 4 168 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 3,787 Reads with adapters: 96 (2.5%) Reads written (passing filters): 3,787 (100.0%) Total basepairs processed: 1,038,723 bp Total written (filtered): 1,038,434 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 96 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.4% C: 34.4% G: 20.8% T: 10.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 95 59.2 0 95 4 1 14.8 0 1