This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 167.52 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 208,819 (3.9%) Reads that were too short: 10,121 (0.2%) Reads written (passing filters): 198,698 (3.7%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 38,998,547 bp (2.4%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 208819 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 120 1302.2 0 120 7 871 1302.2 0 871 8 267 1302.2 0 267 9 6484 1302.2 0 6484 10 21 1302.2 0 21 11 14 1302.2 0 14 12 14 1302.2 0 14 13 10 1302.2 0 10 14 8 1302.2 0 8 15 13 1302.2 0 13 16 15 1302.2 0 15 17 8 1302.2 0 8 18 9 1302.2 0 9 19 9 1302.2 0 9 20 8 1302.2 0 8 21 17 1302.2 0 17 22 17 1302.2 0 17 23 5 1302.2 0 5 24 10 1302.2 0 10 25 22 1302.2 0 22 26 11 1302.2 0 11 27 8 1302.2 0 8 28 10 1302.2 0 10 29 7 1302.2 0 7 30 7 1302.2 0 7 31 18 1302.2 0 18 32 13 1302.2 0 13 33 16 1302.2 0 16 34 20 1302.2 0 20 35 9 1302.2 0 9 36 14 1302.2 0 14 37 46 1302.2 0 46 38 23 1302.2 0 23 39 13 1302.2 0 13 40 47 1302.2 0 47 41 10 1302.2 0 10 42 9 1302.2 0 9 43 8 1302.2 0 8 44 15 1302.2 0 15 45 10 1302.2 0 10 46 42 1302.2 0 42 47 10 1302.2 0 10 48 33 1302.2 0 33 49 38 1302.2 0 38 50 13 1302.2 0 13 51 16 1302.2 0 16 52 17 1302.2 0 17 53 9 1302.2 0 9 54 27 1302.2 0 27 55 61 1302.2 0 61 56 115 1302.2 0 115 57 25 1302.2 0 25 58 812 1302.2 0 812 59 39018 1302.2 0 39018 60 2396 1302.2 0 2396 61 243 1302.2 0 243 62 11 1302.2 0 11 63 15 1302.2 0 15 64 32 1302.2 0 32 65 10 1302.2 0 10 66 13 1302.2 0 13 67 15 1302.2 0 15 68 5 1302.2 0 5 69 9 1302.2 0 9 70 16 1302.2 0 16 71 11 1302.2 0 11 72 9 1302.2 0 9 73 13 1302.2 0 13 74 5 1302.2 0 5 75 19 1302.2 0 19 76 34 1302.2 0 34 77 137 1302.2 0 137 78 35 1302.2 0 35 79 26 1302.2 0 26 80 32 1302.2 0 32 81 24 1302.2 0 24 82 24 1302.2 0 24 83 121 1302.2 0 121 84 69 1302.2 0 69 85 137 1302.2 0 137 86 41 1302.2 0 41 87 169 1302.2 0 169 88 26 1302.2 0 26 89 17 1302.2 0 17 90 23 1302.2 0 23 91 27 1302.2 0 27 92 78 1302.2 0 78 93 86 1302.2 0 86 94 265 1302.2 0 265 95 49 1302.2 0 49 96 168 1302.2 0 168 97 608 1302.2 0 608 98 95 1302.2 0 95 99 168 1302.2 0 168 100 108 1302.2 0 108 101 75 1302.2 0 75 102 211 1302.2 0 211 103 97 1302.2 0 97 104 139 1302.2 0 139 105 112 1302.2 0 112 106 148 1302.2 0 148 107 302 1302.2 0 302 108 508 1302.2 0 508 109 442 1302.2 0 442 110 785 1302.2 0 785 111 812 1302.2 0 812 112 1237 1302.2 0 1237 113 3765 1302.2 0 3765 114 45689 1302.2 0 45689 115 6721 1302.2 0 6721 116 47183 1302.2 0 47183 117 264 1302.2 0 264 118 71 1302.2 0 71 119 46 1302.2 0 46 120 34 1302.2 0 34 121 38 1302.2 0 38 122 54 1302.2 0 54 123 43 1302.2 0 43 124 35 1302.2 0 35 125 34 1302.2 0 34 126 77 1302.2 0 77 127 40 1302.2 0 40 128 573 1302.2 0 573 129 140 1302.2 0 140 130 125 1302.2 0 125 131 75 1302.2 0 75 132 27 1302.2 0 27 133 85 1302.2 0 85 134 44 1302.2 0 44 135 103 1302.2 0 103 136 213 1302.2 0 213 137 180 1302.2 0 180 138 193 1302.2 0 193 139 84 1302.2 0 84 140 211 1302.2 0 211 141 527 1302.2 0 527 142 499 1302.2 0 499 143 302 1302.2 0 302 144 174 1302.2 0 174 145 294 1302.2 0 294 146 415 1302.2 0 415 147 7298 1302.2 0 7298 148 1042 1302.2 0 1042 149 4874 1302.2 0 4874 150 174 1302.2 0 174 151 244 1302.2 0 244 152 533 1302.2 0 533 153 7150 1302.2 0 7150 154 1280 1302.2 0 1280 155 3939 1302.2 0 3939 156 510 1302.2 0 510 157 640 1302.2 0 640 158 199 1302.2 0 199 159 39 1302.2 0 39 160 62 1302.2 0 62 161 79 1302.2 0 79 162 105 1302.2 0 105 163 89 1302.2 0 89 164 87 1302.2 0 87 165 114 1302.2 0 114 166 75 1302.2 0 75 167 121 1302.2 0 121 168 565 1302.2 0 565 169 313 1302.2 0 313 170 251 1302.2 0 251 171 104 1302.2 0 104 172 33 1302.2 0 33 173 15 1302.2 0 15 174 21 1302.2 0 21 175 50 1302.2 0 50 176 82 1302.2 0 82 177 27 1302.2 0 27 178 39 1302.2 0 39 179 27 1302.2 0 27 180 48 1302.2 0 48 181 143 1302.2 0 143 182 123 1302.2 0 123 183 181 1302.2 0 181 184 102 1302.2 0 102 185 155 1302.2 0 155 186 101 1302.2 0 101 187 100 1302.2 0 100 188 24 1302.2 0 24 189 30 1302.2 0 30 190 20 1302.2 0 20 191 30 1302.2 0 30 192 43 1302.2 0 43 193 37 1302.2 0 37 194 41 1302.2 0 41 195 23 1302.2 0 23 196 22 1302.2 0 22 197 45 1302.2 0 45 198 32 1302.2 0 32 199 25 1302.2 0 25 200 24 1302.2 0 24 201 61 1302.2 0 61 202 471 1302.2 0 471 203 137 1302.2 0 137 204 317 1302.2 0 317 205 86 1302.2 0 86 206 44 1302.2 0 44 207 28 1302.2 0 28 208 15 1302.2 0 15 209 29 1302.2 0 29 210 30 1302.2 0 30 211 45 1302.2 0 45 212 58 1302.2 0 58 213 72 1302.2 0 72 214 61 1302.2 0 61 215 80 1302.2 0 80 216 213 1302.2 0 213 217 149 1302.2 0 149 218 156 1302.2 0 156 219 54 1302.2 0 54 220 27 1302.2 0 27 221 34 1302.2 0 34 222 55 1302.2 0 55 223 40 1302.2 0 40 224 53 1302.2 0 53 225 111 1302.2 0 111 226 244 1302.2 0 244 227 227 1302.2 0 227 228 266 1302.2 0 266 229 106 1302.2 0 106 230 47 1302.2 0 47 231 29 1302.2 0 29 232 24 1302.2 0 24 233 54 1302.2 0 54 234 41 1302.2 0 41 235 124 1302.2 0 124 236 108 1302.2 0 108 237 111 1302.2 0 111 238 63 1302.2 0 63 239 21 1302.2 0 21 240 39 1302.2 0 39 241 26 1302.2 0 26 242 24 1302.2 0 24 243 14 1302.2 0 14 244 49 1302.2 0 49 245 64 1302.2 0 64 246 80 1302.2 0 80 247 28 1302.2 0 28 248 23 1302.2 0 23 249 27 1302.2 0 27 250 17 1302.2 0 17 251 38 1302.2 0 38 252 46 1302.2 0 46 253 101 1302.2 0 101 254 184 1302.2 0 184 255 290 1302.2 0 290 256 132 1302.2 0 132 257 56 1302.2 0 56 258 40 1302.2 0 40 259 26 1302.2 0 26 260 18 1302.2 0 18 261 26 1302.2 0 26 262 51 1302.2 0 51 263 51 1302.2 0 51 264 100 1302.2 0 100 265 117 1302.2 0 117 266 101 1302.2 0 101 267 74 1302.2 0 74 268 25 1302.2 0 25 269 35 1302.2 0 35 270 61 1302.2 0 61 271 69 1302.2 0 69 272 95 1302.2 0 95 273 142 1302.2 0 142 274 241 1302.2 0 241 275 442 1302.2 0 442 276 131 1302.2 0 131 277 77 1302.2 0 77 278 88 1302.2 0 88 279 122 1302.2 0 122 280 156 1302.2 0 156 281 264 1302.2 0 264 282 278 1302.2 0 278 283 293 1302.2 0 293 284 86 1302.2 0 86 285 158 1302.2 0 158 286 72 1302.2 0 72 287 65 1302.2 0 65 288 39 1302.2 0 39 289 91 1302.2 0 91 290 287 1302.2 0 287 291 330 1302.2 0 330 292 358 1302.2 0 358 293 158 1302.2 0 158 294 85 1302.2 0 85 295 60 1302.2 0 60 296 21 1302.2 0 21 297 18 1302.2 0 18 298 31 1302.2 0 31 299 26 1302.2 0 26 300 21 1302.2 0 21 301 24 1302.2 0 24 Finished in 8.44 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 198,698 Reads with adapters: 820 (0.4%) Reads that were too short: 33 (0.0%) Reads written (passing filters): 787 (0.4%) Total basepairs processed: 38,998,547 bp Total written (filtered): 201,540 bp (0.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 820 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 7 0.0 3 0 7 17 652 0.0 3 636 15 0 1 18 14 0.0 3 0 3 10 1 19 14 0.0 3 0 0 1 13 20 1 0.0 3 1 36 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 75 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 48 0.0 3 0 0 0 48 86 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 114 4 0.0 3 0 0 0 4 117 3 0.0 3 0 0 0 3 119 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 8 0.0 3 0 0 0 8 134 8 0.0 3 0 0 0 8 135 1 0.0 3 0 0 0 1 136 5 0.0 3 0 0 0 5 137 4 0.0 3 0 0 0 4 140 1 0.0 3 0 0 0 1 141 3 0.0 3 0 0 0 3 160 1 0.0 3 0 0 0 1 170 3 0.0 3 0 0 0 3 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 10 0.0 3 0 0 0 10 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 235 3 0.0 3 0 0 0 3 242 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 787 Reads with adapters: 42 (5.3%) Reads written (passing filters): 787 (100.0%) Total basepairs processed: 201,540 bp Total written (filtered): 201,413 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 42 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 16.7% C: 69.0% G: 11.9% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 41 12.3 0 41 4 1 3.1 0 1