This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 179.53 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 2,978,740 (55.8%) Reads that were too short: 1,039,970 (19.5%) Reads written (passing filters): 1,938,770 (36.3%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 323,093,858 bp (20.1%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 2978740 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 208 1302.2 0 208 7 667 1302.2 0 667 8 343 1302.2 0 343 9 14209 1302.2 0 14209 10 55 1302.2 0 55 11 8 1302.2 0 8 12 2 1302.2 0 2 13 9 1302.2 0 9 14 10 1302.2 0 10 15 4 1302.2 0 4 16 10 1302.2 0 10 17 7 1302.2 0 7 18 8 1302.2 0 8 19 4 1302.2 0 4 20 3 1302.2 0 3 21 3 1302.2 0 3 22 12 1302.2 0 12 23 17 1302.2 0 17 24 10 1302.2 0 10 25 19 1302.2 0 19 26 6 1302.2 0 6 27 11 1302.2 0 11 28 12 1302.2 0 12 29 7 1302.2 0 7 30 14 1302.2 0 14 31 8 1302.2 0 8 32 8 1302.2 0 8 33 5 1302.2 0 5 34 6 1302.2 0 6 35 15 1302.2 0 15 36 8 1302.2 0 8 37 10 1302.2 0 10 38 5 1302.2 0 5 39 20 1302.2 0 20 40 20 1302.2 0 20 41 65 1302.2 0 65 42 102 1302.2 0 102 43 162 1302.2 0 162 44 17 1302.2 0 17 45 8 1302.2 0 8 46 5 1302.2 0 5 47 12 1302.2 0 12 48 9 1302.2 0 9 49 17 1302.2 0 17 50 20 1302.2 0 20 51 29 1302.2 0 29 52 64 1302.2 0 64 53 12 1302.2 0 12 54 7 1302.2 0 7 55 8 1302.2 0 8 56 13 1302.2 0 13 57 18 1302.2 0 18 58 16 1302.2 0 16 59 10 1302.2 0 10 60 16 1302.2 0 16 61 14 1302.2 0 14 62 230 1302.2 0 230 63 108 1302.2 0 108 64 146 1302.2 0 146 65 84 1302.2 0 84 66 364 1302.2 0 364 67 131 1302.2 0 131 68 337 1302.2 0 337 69 164 1302.2 0 164 70 343 1302.2 0 343 71 2038 1302.2 0 2038 72 8901 1302.2 0 8901 73 33224 1302.2 0 33224 74 9968 1302.2 0 9968 75 14788 1302.2 0 14788 76 7455 1302.2 0 7455 77 8650 1302.2 0 8650 78 14740 1302.2 0 14740 79 10273 1302.2 0 10273 80 15050 1302.2 0 15050 81 9009 1302.2 0 9009 82 8350 1302.2 0 8350 83 5821 1302.2 0 5821 84 5724 1302.2 0 5724 85 8314 1302.2 0 8314 86 9826 1302.2 0 9826 87 4204 1302.2 0 4204 88 5665 1302.2 0 5665 89 19600 1302.2 0 19600 90 9670 1302.2 0 9670 91 29778 1302.2 0 29778 92 9324 1302.2 0 9324 93 12510 1302.2 0 12510 94 12037 1302.2 0 12037 95 30828 1302.2 0 30828 96 13243 1302.2 0 13243 97 8685 1302.2 0 8685 98 4371 1302.2 0 4371 99 7419 1302.2 0 7419 100 7598 1302.2 0 7598 101 17182 1302.2 0 17182 102 16760 1302.2 0 16760 103 20272 1302.2 0 20272 104 13645 1302.2 0 13645 105 47112 1302.2 0 47112 106 42763 1302.2 0 42763 107 28810 1302.2 0 28810 108 14095 1302.2 0 14095 109 10700 1302.2 0 10700 110 9428 1302.2 0 9428 111 21217 1302.2 0 21217 112 18144 1302.2 0 18144 113 22034 1302.2 0 22034 114 20933 1302.2 0 20933 115 21348 1302.2 0 21348 116 8745 1302.2 0 8745 117 12135 1302.2 0 12135 118 12163 1302.2 0 12163 119 17371 1302.2 0 17371 120 16486 1302.2 0 16486 121 25751 1302.2 0 25751 122 13073 1302.2 0 13073 123 22533 1302.2 0 22533 124 18674 1302.2 0 18674 125 19273 1302.2 0 19273 126 23585 1302.2 0 23585 127 21091 1302.2 0 21091 128 15693 1302.2 0 15693 129 10547 1302.2 0 10547 130 14165 1302.2 0 14165 131 9744 1302.2 0 9744 132 16725 1302.2 0 16725 133 20010 1302.2 0 20010 134 20679 1302.2 0 20679 135 17317 1302.2 0 17317 136 13700 1302.2 0 13700 137 11329 1302.2 0 11329 138 10256 1302.2 0 10256 139 10577 1302.2 0 10577 140 12916 1302.2 0 12916 141 15913 1302.2 0 15913 142 10995 1302.2 0 10995 143 12879 1302.2 0 12879 144 11026 1302.2 0 11026 145 17047 1302.2 0 17047 146 17405 1302.2 0 17405 147 13466 1302.2 0 13466 148 20607 1302.2 0 20607 149 16635 1302.2 0 16635 150 19388 1302.2 0 19388 151 11182 1302.2 0 11182 152 15241 1302.2 0 15241 153 19936 1302.2 0 19936 154 18594 1302.2 0 18594 155 14910 1302.2 0 14910 156 12088 1302.2 0 12088 157 10784 1302.2 0 10784 158 13449 1302.2 0 13449 159 12198 1302.2 0 12198 160 13152 1302.2 0 13152 161 15335 1302.2 0 15335 162 18545 1302.2 0 18545 163 18471 1302.2 0 18471 164 17765 1302.2 0 17765 165 14463 1302.2 0 14463 166 12121 1302.2 0 12121 167 10212 1302.2 0 10212 168 10264 1302.2 0 10264 169 13113 1302.2 0 13113 170 13127 1302.2 0 13127 171 10297 1302.2 0 10297 172 16090 1302.2 0 16090 173 14478 1302.2 0 14478 174 27623 1302.2 0 27623 175 14179 1302.2 0 14179 176 11903 1302.2 0 11903 177 6993 1302.2 0 6993 178 11937 1302.2 0 11937 179 12005 1302.2 0 12005 180 14975 1302.2 0 14975 181 13021 1302.2 0 13021 182 10095 1302.2 0 10095 183 11613 1302.2 0 11613 184 11536 1302.2 0 11536 185 14488 1302.2 0 14488 186 18023 1302.2 0 18023 187 14396 1302.2 0 14396 188 12994 1302.2 0 12994 189 10670 1302.2 0 10670 190 13505 1302.2 0 13505 191 15986 1302.2 0 15986 192 16642 1302.2 0 16642 193 16543 1302.2 0 16543 194 14453 1302.2 0 14453 195 6606 1302.2 0 6606 196 7285 1302.2 0 7285 197 7975 1302.2 0 7975 198 15171 1302.2 0 15171 199 22810 1302.2 0 22810 200 11980 1302.2 0 11980 201 7849 1302.2 0 7849 202 7919 1302.2 0 7919 203 8418 1302.2 0 8418 204 9634 1302.2 0 9634 205 10900 1302.2 0 10900 206 12598 1302.2 0 12598 207 9776 1302.2 0 9776 208 8773 1302.2 0 8773 209 11265 1302.2 0 11265 210 8490 1302.2 0 8490 211 13354 1302.2 0 13354 212 8341 1302.2 0 8341 213 12429 1302.2 0 12429 214 9803 1302.2 0 9803 215 10849 1302.2 0 10849 216 9561 1302.2 0 9561 217 10016 1302.2 0 10016 218 13403 1302.2 0 13403 219 15104 1302.2 0 15104 220 11989 1302.2 0 11989 221 7805 1302.2 0 7805 222 8564 1302.2 0 8564 223 9197 1302.2 0 9197 224 11051 1302.2 0 11051 225 13551 1302.2 0 13551 226 11883 1302.2 0 11883 227 14946 1302.2 0 14946 228 11882 1302.2 0 11882 229 12775 1302.2 0 12775 230 14249 1302.2 0 14249 231 12241 1302.2 0 12241 232 8109 1302.2 0 8109 233 8152 1302.2 0 8152 234 12451 1302.2 0 12451 235 13562 1302.2 0 13562 236 15383 1302.2 0 15383 237 13117 1302.2 0 13117 238 12820 1302.2 0 12820 239 13320 1302.2 0 13320 240 10645 1302.2 0 10645 241 7564 1302.2 0 7564 242 8454 1302.2 0 8454 243 7731 1302.2 0 7731 244 8562 1302.2 0 8562 245 10391 1302.2 0 10391 246 12207 1302.2 0 12207 247 16922 1302.2 0 16922 248 15871 1302.2 0 15871 249 13752 1302.2 0 13752 250 10210 1302.2 0 10210 251 7969 1302.2 0 7969 252 6943 1302.2 0 6943 253 9355 1302.2 0 9355 254 10272 1302.2 0 10272 255 13677 1302.2 0 13677 256 14559 1302.2 0 14559 257 16663 1302.2 0 16663 258 11919 1302.2 0 11919 259 12640 1302.2 0 12640 260 9782 1302.2 0 9782 261 9643 1302.2 0 9643 262 7769 1302.2 0 7769 263 6424 1302.2 0 6424 264 7326 1302.2 0 7326 265 7514 1302.2 0 7514 266 10277 1302.2 0 10277 267 11008 1302.2 0 11008 268 9108 1302.2 0 9108 269 9184 1302.2 0 9184 270 8734 1302.2 0 8734 271 9556 1302.2 0 9556 272 10174 1302.2 0 10174 273 13963 1302.2 0 13963 274 12337 1302.2 0 12337 275 10457 1302.2 0 10457 276 7382 1302.2 0 7382 277 6850 1302.2 0 6850 278 8430 1302.2 0 8430 279 11919 1302.2 0 11919 280 13497 1302.2 0 13497 281 9708 1302.2 0 9708 282 12414 1302.2 0 12414 283 8246 1302.2 0 8246 284 12112 1302.2 0 12112 285 12853 1302.2 0 12853 286 11575 1302.2 0 11575 287 9920 1302.2 0 9920 288 7431 1302.2 0 7431 289 6993 1302.2 0 6993 290 7239 1302.2 0 7239 291 9053 1302.2 0 9053 292 8486 1302.2 0 8486 293 10060 1302.2 0 10060 294 9916 1302.2 0 9916 295 9389 1302.2 0 9389 296 7850 1302.2 0 7850 297 6205 1302.2 0 6205 298 4812 1302.2 0 4812 299 3736 1302.2 0 3736 300 6809 1302.2 0 6809 301 9843 1302.2 0 9843 Finished in 83.20 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 1,938,770 Reads with adapters: 1,574 (0.1%) Reads that were too short: 689 (0.0%) Reads written (passing filters): 885 (0.0%) Total basepairs processed: 323,093,858 bp Total written (filtered): 197,867 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1574 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 6 0.0 3 0 0 0 1 5 19 11 0.0 3 0 7 0 0 4 20 450 0.0 4 433 7 2 5 3 21 12 0.0 4 1 0 1 2 8 22 102 0.0 4 85 4 0 0 13 23 5 0.0 4 0 0 0 2 3 24 5 0.0 4 0 0 0 0 5 25 7 0.0 4 0 0 0 0 7 26 9 0.0 4 0 0 0 0 9 27 10 0.0 4 0 0 0 0 10 28 7 0.0 4 0 0 0 0 7 29 9 0.0 4 0 0 0 0 9 30 4 0.0 4 0 0 0 0 4 31 2 0.0 4 0 0 0 0 2 32 3 0.0 4 0 0 0 0 3 33 6 0.0 4 0 0 0 0 6 34 5 0.0 4 0 0 0 0 5 35 4 0.0 4 0 0 0 0 4 36 11 0.0 4 0 0 0 0 11 37 7 0.0 4 0 0 0 0 7 38 9 0.0 4 0 0 0 0 9 39 10 0.0 4 0 0 0 0 10 40 12 0.0 4 0 0 0 0 12 41 9 0.0 4 0 0 0 0 9 42 5 0.0 4 0 0 0 0 5 43 6 0.0 4 0 0 0 0 6 44 5 0.0 4 0 0 0 0 5 45 7 0.0 4 0 0 0 0 7 46 11 0.0 4 0 0 0 0 11 47 3 0.0 4 0 0 0 0 3 48 6 0.0 4 0 0 0 0 6 49 3 0.0 4 0 0 0 0 3 50 11 0.0 4 0 0 0 1 10 51 8 0.0 4 0 0 0 1 7 52 9 0.0 4 0 0 0 0 9 53 4 0.0 4 0 0 0 0 4 54 8 0.0 4 0 0 0 0 8 55 10 0.0 4 0 0 0 0 10 56 10 0.0 4 0 0 0 0 10 57 5 0.0 4 0 0 0 0 5 58 4 0.0 4 0 0 0 1 3 59 9 0.0 4 0 0 0 0 9 60 8 0.0 4 0 0 0 1 7 61 12 0.0 4 0 0 0 1 11 62 16 0.0 4 0 0 0 0 16 63 5 0.0 4 0 0 0 0 5 64 4 0.0 4 0 0 0 0 4 65 12 0.0 4 0 0 0 2 10 66 8 0.0 4 0 0 0 0 8 67 6 0.0 4 0 0 0 0 6 68 3 0.0 4 0 0 0 0 3 69 7 0.0 4 0 0 0 1 6 70 3 0.0 4 0 0 0 0 3 71 6 0.0 4 0 0 0 0 6 72 7 0.0 4 0 0 0 0 7 73 6 0.0 4 0 0 0 0 6 74 5 0.0 4 0 0 0 0 5 75 5 0.0 4 0 0 0 0 5 76 6 0.0 4 0 0 0 0 6 77 11 0.0 4 0 0 0 1 10 78 9 0.0 4 0 0 0 0 9 79 3 0.0 4 0 0 0 0 3 80 5 0.0 4 0 0 0 0 5 81 6 0.0 4 0 0 0 0 6 82 4 0.0 4 0 0 0 0 4 83 8 0.0 4 0 0 0 0 8 84 5 0.0 4 0 0 0 0 5 85 5 0.0 4 0 0 0 0 5 86 7 0.0 4 0 0 0 0 7 87 2 0.0 4 0 0 0 0 2 88 16 0.0 4 0 0 0 0 16 89 6 0.0 4 0 0 0 0 6 90 8 0.0 4 0 0 0 0 8 91 7 0.0 4 0 0 0 0 7 92 7 0.0 4 0 0 0 0 7 93 11 0.0 4 0 0 0 0 11 94 3 0.0 4 0 0 0 1 2 95 7 0.0 4 0 0 0 0 7 96 7 0.0 4 0 0 0 1 6 97 6 0.0 4 0 0 0 0 6 98 6 0.0 4 0 0 0 0 6 99 10 0.0 4 0 0 0 0 10 100 6 0.0 4 0 0 0 0 6 101 4 0.0 4 0 0 0 0 4 102 7 0.0 4 0 0 0 0 7 103 4 0.0 4 0 0 0 0 4 104 9 0.0 4 0 0 0 0 9 105 8 0.0 4 0 0 0 0 8 106 3 0.0 4 0 0 0 0 3 107 7 0.0 4 0 0 0 0 7 108 7 0.0 4 0 0 0 0 7 109 7 0.0 4 0 0 0 0 7 110 3 0.0 4 0 0 0 0 3 111 4 0.0 4 0 0 0 0 4 112 12 0.0 4 0 0 0 0 12 113 3 0.0 4 0 0 0 0 3 114 4 0.0 4 0 0 0 0 4 115 3 0.0 4 0 0 0 1 2 116 8 0.0 4 0 0 0 0 8 117 6 0.0 4 0 0 0 0 6 118 4 0.0 4 0 0 0 0 4 119 11 0.0 4 0 0 0 2 9 120 6 0.0 4 0 0 0 0 6 121 5 0.0 4 0 0 0 0 5 122 5 0.0 4 0 0 0 0 5 123 6 0.0 4 0 0 0 0 6 124 3 0.0 4 0 0 0 0 3 125 7 0.0 4 0 0 0 0 7 126 10 0.0 4 0 0 0 0 10 127 15 0.0 4 0 0 0 0 15 128 7 0.0 4 0 0 0 0 7 129 7 0.0 4 0 0 0 1 6 130 4 0.0 4 0 0 0 0 4 131 3 0.0 4 0 0 0 0 3 132 6 0.0 4 0 0 0 0 6 133 3 0.0 4 0 0 0 0 3 134 2 0.0 4 0 0 0 0 2 135 7 0.0 4 0 0 0 0 7 136 5 0.0 4 0 0 0 0 5 137 3 0.0 4 0 0 0 0 3 138 5 0.0 4 0 0 0 0 5 139 9 0.0 4 0 0 0 1 8 140 7 0.0 4 0 0 0 0 7 141 4 0.0 4 0 0 0 1 3 142 8 0.0 4 0 0 0 0 8 143 6 0.0 4 0 0 0 0 6 144 5 0.0 4 0 0 0 0 5 145 3 0.0 4 0 0 0 0 3 146 5 0.0 4 0 0 0 0 5 147 6 0.0 4 0 0 0 0 6 148 3 0.0 4 0 0 0 0 3 149 4 0.0 4 0 0 0 0 4 150 3 0.0 4 0 0 0 0 3 151 4 0.0 4 0 0 0 0 4 152 2 0.0 4 0 0 0 0 2 153 5 0.0 4 0 0 0 1 4 154 3 0.0 4 0 0 0 0 3 155 4 0.0 4 0 0 0 0 4 156 2 0.0 4 0 0 0 1 1 157 5 0.0 4 0 0 0 0 5 158 3 0.0 4 0 0 0 0 3 159 5 0.0 4 0 0 0 0 5 160 2 0.0 4 0 0 0 1 1 161 2 0.0 4 0 0 0 0 2 162 3 0.0 4 0 0 0 0 3 163 4 0.0 4 0 0 0 0 4 164 1 0.0 4 0 0 0 0 1 165 5 0.0 4 0 0 0 1 4 166 3 0.0 4 0 0 0 0 3 167 4 0.0 4 0 0 0 0 4 168 3 0.0 4 0 0 0 0 3 169 5 0.0 4 0 0 0 0 5 170 3 0.0 4 0 0 0 0 3 171 5 0.0 4 0 0 0 0 5 172 2 0.0 4 0 0 0 0 2 173 7 0.0 4 0 0 0 0 7 174 3 0.0 4 0 0 0 0 3 175 4 0.0 4 0 0 0 0 4 176 3 0.0 4 0 0 0 0 3 177 4 0.0 4 0 0 0 0 4 178 4 0.0 4 0 0 0 0 4 179 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 181 2 0.0 4 0 0 0 0 2 182 5 0.0 4 0 0 0 0 5 183 2 0.0 4 0 0 0 0 2 184 3 0.0 4 0 0 0 0 3 185 1 0.0 4 0 0 0 0 1 186 3 0.0 4 0 0 0 0 3 188 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 191 2 0.0 4 0 0 0 0 2 192 3 0.0 4 0 0 0 0 3 193 2 0.0 4 0 0 0 0 2 195 2 0.0 4 0 0 0 0 2 197 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 200 4 0.0 4 0 0 0 0 4 202 2 0.0 4 0 0 0 0 2 204 2 0.0 4 0 0 0 0 2 205 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 212 3 0.0 4 0 0 0 0 3 213 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 220 4 0.0 4 0 0 0 0 4 223 2 0.0 4 0 0 0 0 2 224 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 885 Reads with adapters: 110 (12.4%) Reads written (passing filters): 885 (100.0%) Total basepairs processed: 197,867 bp Total written (filtered): 175,598 bp (88.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 110 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 71.8% C: 0.9% G: 20.9% T: 4.5% none/other: 1.8% Overview of removed sequences length count expect max.err error counts 3 2 13.8 0 2 11 23 0.0 1 0 23 12 1 0.0 1 0 1 261 76 0.0 1 71 5 262 1 0.0 1 1 264 1 0.0 1 1 269 1 0.0 1 0 1 273 3 0.0 1 3 274 2 0.0 1 2