This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 159.78 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 192,481 (3.6%) Reads that were too short: 181,412 (3.4%) Reads written (passing filters): 11,069 (0.2%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 2,474,022 bp (0.2%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 192481 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 54 1302.2 0 54 7 99 1302.2 0 99 8 3331 1302.2 0 3331 9 938 1302.2 0 938 10 23 1302.2 0 23 11 18 1302.2 0 18 12 12 1302.2 0 12 13 18 1302.2 0 18 14 13 1302.2 0 13 15 19 1302.2 0 19 16 19 1302.2 0 19 17 25 1302.2 0 25 18 99 1302.2 0 99 19 59 1302.2 0 59 20 27 1302.2 0 27 21 19 1302.2 0 19 22 37 1302.2 0 37 23 16 1302.2 0 16 24 21 1302.2 0 21 25 16 1302.2 0 16 26 21 1302.2 0 21 27 18 1302.2 0 18 28 13 1302.2 0 13 29 17 1302.2 0 17 30 19 1302.2 0 19 31 31 1302.2 0 31 32 12 1302.2 0 12 33 113 1302.2 0 113 34 15 1302.2 0 15 35 16 1302.2 0 16 36 10 1302.2 0 10 37 18 1302.2 0 18 38 25 1302.2 0 25 39 16 1302.2 0 16 40 20 1302.2 0 20 41 15 1302.2 0 15 42 54 1302.2 0 54 43 22 1302.2 0 22 44 27 1302.2 0 27 45 206 1302.2 0 206 46 25 1302.2 0 25 47 18 1302.2 0 18 48 22 1302.2 0 22 49 20 1302.2 0 20 50 22 1302.2 0 22 51 46 1302.2 0 46 52 174 1302.2 0 174 53 48 1302.2 0 48 54 19 1302.2 0 19 55 22 1302.2 0 22 56 14 1302.2 0 14 57 26 1302.2 0 26 58 28 1302.2 0 28 59 10 1302.2 0 10 60 10 1302.2 0 10 61 18 1302.2 0 18 62 18 1302.2 0 18 63 47 1302.2 0 47 64 53 1302.2 0 53 65 31 1302.2 0 31 66 14 1302.2 0 14 67 22 1302.2 0 22 68 16 1302.2 0 16 69 23 1302.2 0 23 70 11 1302.2 0 11 71 6 1302.2 0 6 72 3 1302.2 0 3 73 14 1302.2 0 14 74 15 1302.2 0 15 75 20 1302.2 0 20 76 16 1302.2 0 16 77 26 1302.2 0 26 78 18 1302.2 0 18 79 12 1302.2 0 12 80 10 1302.2 0 10 81 21 1302.2 0 21 82 40 1302.2 0 40 83 13 1302.2 0 13 84 39 1302.2 0 39 85 57 1302.2 0 57 86 26 1302.2 0 26 87 24 1302.2 0 24 88 22 1302.2 0 22 89 30 1302.2 0 30 90 9 1302.2 0 9 91 29 1302.2 0 29 92 33 1302.2 0 33 93 36 1302.2 0 36 94 66 1302.2 0 66 95 60 1302.2 0 60 96 25 1302.2 0 25 97 26 1302.2 0 26 98 17 1302.2 0 17 99 23 1302.2 0 23 100 9 1302.2 0 9 101 17 1302.2 0 17 102 12 1302.2 0 12 103 16 1302.2 0 16 104 11 1302.2 0 11 105 13 1302.2 0 13 106 13 1302.2 0 13 107 7 1302.2 0 7 108 5 1302.2 0 5 109 21 1302.2 0 21 110 8 1302.2 0 8 111 11 1302.2 0 11 112 6 1302.2 0 6 113 13 1302.2 0 13 114 7 1302.2 0 7 115 15 1302.2 0 15 116 11 1302.2 0 11 117 10 1302.2 0 10 118 9 1302.2 0 9 119 9 1302.2 0 9 120 23 1302.2 0 23 121 13 1302.2 0 13 122 19 1302.2 0 19 123 31 1302.2 0 31 124 29 1302.2 0 29 125 24 1302.2 0 24 126 70 1302.2 0 70 127 31 1302.2 0 31 128 16 1302.2 0 16 129 26 1302.2 0 26 130 22 1302.2 0 22 131 53 1302.2 0 53 132 359 1302.2 0 359 133 44 1302.2 0 44 134 27 1302.2 0 27 135 20 1302.2 0 20 136 24 1302.2 0 24 137 8 1302.2 0 8 138 16 1302.2 0 16 139 27 1302.2 0 27 140 15 1302.2 0 15 141 26 1302.2 0 26 142 9 1302.2 0 9 143 18 1302.2 0 18 144 17 1302.2 0 17 145 14 1302.2 0 14 146 20 1302.2 0 20 147 16 1302.2 0 16 148 21 1302.2 0 21 149 15 1302.2 0 15 150 5 1302.2 0 5 151 11 1302.2 0 11 152 34 1302.2 0 34 153 7 1302.2 0 7 154 12 1302.2 0 12 155 19 1302.2 0 19 156 18 1302.2 0 18 157 12 1302.2 0 12 158 11 1302.2 0 11 159 14 1302.2 0 14 160 10 1302.2 0 10 161 10 1302.2 0 10 162 9 1302.2 0 9 163 11 1302.2 0 11 164 16 1302.2 0 16 165 23 1302.2 0 23 166 28 1302.2 0 28 167 17 1302.2 0 17 168 12 1302.2 0 12 169 8 1302.2 0 8 170 10 1302.2 0 10 171 20 1302.2 0 20 172 21 1302.2 0 21 173 35 1302.2 0 35 174 29 1302.2 0 29 175 18 1302.2 0 18 176 30 1302.2 0 30 177 19 1302.2 0 19 178 12 1302.2 0 12 179 22 1302.2 0 22 180 38 1302.2 0 38 181 73 1302.2 0 73 182 33 1302.2 0 33 183 27 1302.2 0 27 184 18 1302.2 0 18 185 53 1302.2 0 53 186 39 1302.2 0 39 187 56 1302.2 0 56 188 27 1302.2 0 27 189 22 1302.2 0 22 190 21 1302.2 0 21 191 42 1302.2 0 42 192 99 1302.2 0 99 193 559 1302.2 0 559 194 452 1302.2 0 452 195 407 1302.2 0 407 196 53 1302.2 0 53 197 22 1302.2 0 22 198 15 1302.2 0 15 199 17 1302.2 0 17 200 77 1302.2 0 77 201 96 1302.2 0 96 202 28 1302.2 0 28 203 18 1302.2 0 18 204 14 1302.2 0 14 205 17 1302.2 0 17 206 32 1302.2 0 32 207 32 1302.2 0 32 208 22 1302.2 0 22 209 15 1302.2 0 15 210 30 1302.2 0 30 211 67 1302.2 0 67 212 72 1302.2 0 72 213 47 1302.2 0 47 214 51 1302.2 0 51 215 42 1302.2 0 42 216 32 1302.2 0 32 217 46 1302.2 0 46 218 40 1302.2 0 40 219 70 1302.2 0 70 220 67 1302.2 0 67 221 135 1302.2 0 135 222 115 1302.2 0 115 223 146 1302.2 0 146 224 506 1302.2 0 506 225 248 1302.2 0 248 226 244 1302.2 0 244 227 466 1302.2 0 466 228 275 1302.2 0 275 229 231 1302.2 0 231 230 301 1302.2 0 301 231 412 1302.2 0 412 232 1392 1302.2 0 1392 233 2319 1302.2 0 2319 234 3657 1302.2 0 3657 235 939 1302.2 0 939 236 582 1302.2 0 582 237 5474 1302.2 0 5474 238 10988 1302.2 0 10988 239 26797 1302.2 0 26797 240 3093 1302.2 0 3093 241 4281 1302.2 0 4281 242 615 1302.2 0 615 243 341 1302.2 0 341 244 739 1302.2 0 739 245 286 1302.2 0 286 246 327 1302.2 0 327 247 587 1302.2 0 587 248 362 1302.2 0 362 249 455 1302.2 0 455 250 747 1302.2 0 747 251 1111 1302.2 0 1111 252 3011 1302.2 0 3011 253 2650 1302.2 0 2650 254 4390 1302.2 0 4390 255 1026 1302.2 0 1026 256 1022 1302.2 0 1022 257 7913 1302.2 0 7913 258 40195 1302.2 0 40195 259 44682 1302.2 0 44682 260 5209 1302.2 0 5209 261 35 1302.2 0 35 262 19 1302.2 0 19 263 4 1302.2 0 4 264 12 1302.2 0 12 265 39 1302.2 0 39 266 9 1302.2 0 9 267 14 1302.2 0 14 268 11 1302.2 0 11 269 13 1302.2 0 13 270 8 1302.2 0 8 271 5 1302.2 0 5 272 9 1302.2 0 9 273 6 1302.2 0 6 274 8 1302.2 0 8 275 7 1302.2 0 7 276 11 1302.2 0 11 277 7 1302.2 0 7 278 7 1302.2 0 7 279 8 1302.2 0 8 280 8 1302.2 0 8 281 11 1302.2 0 11 282 48 1302.2 0 48 283 38 1302.2 0 38 284 27 1302.2 0 27 285 90 1302.2 0 90 286 357 1302.2 0 357 287 603 1302.2 0 603 288 18 1302.2 0 18 289 14 1302.2 0 14 290 14 1302.2 0 14 291 88 1302.2 0 88 292 69 1302.2 0 69 293 60 1302.2 0 60 294 13 1302.2 0 13 295 6 1302.2 0 6 296 4 1302.2 0 4 297 74 1302.2 0 74 298 105 1302.2 0 105 299 26 1302.2 0 26 300 17 1302.2 0 17 301 479 1302.2 0 479 Finished in 0.60 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 11,069 Reads with adapters: 929 (8.4%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 924 (8.3%) Total basepairs processed: 2,474,022 bp Total written (filtered): 248,851 bp (10.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 929 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 0 1 19 29 0.0 3 0 20 0 1 8 20 836 0.0 4 797 24 0 7 8 21 19 0.0 4 0 3 0 15 1 22 12 0.0 4 1 0 0 0 11 23 3 0.0 4 0 0 0 2 1 41 1 0.0 4 0 0 0 0 1 71 1 0.0 4 1 127 22 0.0 4 0 0 0 0 22 161 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 1 212 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 924 Reads with adapters: 76 (8.2%) Reads written (passing filters): 924 (100.0%) Total basepairs processed: 248,851 bp Total written (filtered): 247,812 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 76 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 3.9% G: 89.5% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 13 0.0 0 0 13 10 17 0.0 1 0 17 11 45 0.0 1 0 45 257 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.