This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 176.09 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 131,316 (2.5%) Reads that were too short: 11,712 (0.2%) Reads written (passing filters): 119,604 (2.2%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 26,264,401 bp (1.6%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 131316 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 164 1302.2 0 164 7 263 1302.2 0 263 8 170 1302.2 0 170 9 26 1302.2 0 26 10 27 1302.2 0 27 11 17 1302.2 0 17 12 52 1302.2 0 52 13 20 1302.2 0 20 14 33 1302.2 0 33 15 30 1302.2 0 30 16 30 1302.2 0 30 17 24 1302.2 0 24 18 30 1302.2 0 30 19 38 1302.2 0 38 20 36 1302.2 0 36 21 36 1302.2 0 36 22 19 1302.2 0 19 23 27 1302.2 0 27 24 39 1302.2 0 39 25 24 1302.2 0 24 26 46 1302.2 0 46 27 29 1302.2 0 29 28 30 1302.2 0 30 29 97 1302.2 0 97 30 27 1302.2 0 27 31 22 1302.2 0 22 32 39 1302.2 0 39 33 26 1302.2 0 26 34 22 1302.2 0 22 35 32 1302.2 0 32 36 39 1302.2 0 39 37 41 1302.2 0 41 38 83 1302.2 0 83 39 203 1302.2 0 203 40 112 1302.2 0 112 41 208 1302.2 0 208 42 39 1302.2 0 39 43 41 1302.2 0 41 44 75 1302.2 0 75 45 42 1302.2 0 42 46 44 1302.2 0 44 47 46 1302.2 0 46 48 45 1302.2 0 45 49 125 1302.2 0 125 50 187 1302.2 0 187 51 307 1302.2 0 307 52 683 1302.2 0 683 53 632 1302.2 0 632 54 1087 1302.2 0 1087 55 3272 1302.2 0 3272 56 56688 1302.2 0 56688 57 3292 1302.2 0 3292 58 20181 1302.2 0 20181 59 100 1302.2 0 100 60 27 1302.2 0 27 61 14 1302.2 0 14 62 29 1302.2 0 29 63 28 1302.2 0 28 64 21 1302.2 0 21 65 18 1302.2 0 18 66 23 1302.2 0 23 67 29 1302.2 0 29 68 17 1302.2 0 17 69 17 1302.2 0 17 70 20 1302.2 0 20 71 17 1302.2 0 17 72 18 1302.2 0 18 73 18 1302.2 0 18 74 22 1302.2 0 22 75 30 1302.2 0 30 76 25 1302.2 0 25 77 23 1302.2 0 23 78 25 1302.2 0 25 79 38 1302.2 0 38 80 34 1302.2 0 34 81 56 1302.2 0 56 82 57 1302.2 0 57 83 264 1302.2 0 264 84 129 1302.2 0 129 85 147 1302.2 0 147 86 43 1302.2 0 43 87 158 1302.2 0 158 88 188 1302.2 0 188 89 202 1302.2 0 202 90 45 1302.2 0 45 91 23 1302.2 0 23 92 24 1302.2 0 24 93 49 1302.2 0 49 94 103 1302.2 0 103 95 76 1302.2 0 76 96 83 1302.2 0 83 97 13 1302.2 0 13 98 23 1302.2 0 23 99 25 1302.2 0 25 100 28 1302.2 0 28 101 95 1302.2 0 95 102 54 1302.2 0 54 103 97 1302.2 0 97 104 102 1302.2 0 102 105 104 1302.2 0 104 106 23 1302.2 0 23 107 44 1302.2 0 44 108 30 1302.2 0 30 109 25 1302.2 0 25 110 30 1302.2 0 30 111 64 1302.2 0 64 112 29 1302.2 0 29 113 30 1302.2 0 30 114 55 1302.2 0 55 115 37 1302.2 0 37 116 48 1302.2 0 48 117 58 1302.2 0 58 118 52 1302.2 0 52 119 282 1302.2 0 282 120 151 1302.2 0 151 121 107 1302.2 0 107 122 97 1302.2 0 97 123 113 1302.2 0 113 124 761 1302.2 0 761 125 969 1302.2 0 969 126 2996 1302.2 0 2996 127 109 1302.2 0 109 128 217 1302.2 0 217 129 38 1302.2 0 38 130 45 1302.2 0 45 131 60 1302.2 0 60 132 103 1302.2 0 103 133 72 1302.2 0 72 134 78 1302.2 0 78 135 57 1302.2 0 57 136 69 1302.2 0 69 137 66 1302.2 0 66 138 95 1302.2 0 95 139 105 1302.2 0 105 140 72 1302.2 0 72 141 78 1302.2 0 78 142 45 1302.2 0 45 143 62 1302.2 0 62 144 66 1302.2 0 66 145 127 1302.2 0 127 146 142 1302.2 0 142 147 563 1302.2 0 563 148 370 1302.2 0 370 149 949 1302.2 0 949 150 1226 1302.2 0 1226 151 239 1302.2 0 239 152 211 1302.2 0 211 153 69 1302.2 0 69 154 87 1302.2 0 87 155 53 1302.2 0 53 156 47 1302.2 0 47 157 72 1302.2 0 72 158 175 1302.2 0 175 159 148 1302.2 0 148 160 294 1302.2 0 294 161 110 1302.2 0 110 162 147 1302.2 0 147 163 88 1302.2 0 88 164 108 1302.2 0 108 165 218 1302.2 0 218 166 241 1302.2 0 241 167 729 1302.2 0 729 168 711 1302.2 0 711 169 879 1302.2 0 879 170 276 1302.2 0 276 171 448 1302.2 0 448 172 697 1302.2 0 697 173 251 1302.2 0 251 174 295 1302.2 0 295 175 199 1302.2 0 199 176 251 1302.2 0 251 177 364 1302.2 0 364 178 275 1302.2 0 275 179 128 1302.2 0 128 180 161 1302.2 0 161 181 233 1302.2 0 233 182 143 1302.2 0 143 183 111 1302.2 0 111 184 120 1302.2 0 120 185 133 1302.2 0 133 186 128 1302.2 0 128 187 80 1302.2 0 80 188 83 1302.2 0 83 189 126 1302.2 0 126 190 155 1302.2 0 155 191 240 1302.2 0 240 192 398 1302.2 0 398 193 232 1302.2 0 232 194 165 1302.2 0 165 195 340 1302.2 0 340 196 823 1302.2 0 823 197 1155 1302.2 0 1155 198 381 1302.2 0 381 199 427 1302.2 0 427 200 2632 1302.2 0 2632 201 985 1302.2 0 985 202 1610 1302.2 0 1610 203 86 1302.2 0 86 204 68 1302.2 0 68 205 103 1302.2 0 103 206 63 1302.2 0 63 207 90 1302.2 0 90 208 46 1302.2 0 46 209 45 1302.2 0 45 210 42 1302.2 0 42 211 55 1302.2 0 55 212 74 1302.2 0 74 213 121 1302.2 0 121 214 80 1302.2 0 80 215 114 1302.2 0 114 216 125 1302.2 0 125 217 107 1302.2 0 107 218 112 1302.2 0 112 219 222 1302.2 0 222 220 106 1302.2 0 106 221 144 1302.2 0 144 222 63 1302.2 0 63 223 79 1302.2 0 79 224 237 1302.2 0 237 225 176 1302.2 0 176 226 126 1302.2 0 126 227 127 1302.2 0 127 228 170 1302.2 0 170 229 399 1302.2 0 399 230 162 1302.2 0 162 231 190 1302.2 0 190 232 384 1302.2 0 384 233 235 1302.2 0 235 234 245 1302.2 0 245 235 88 1302.2 0 88 236 53 1302.2 0 53 237 31 1302.2 0 31 238 39 1302.2 0 39 239 34 1302.2 0 34 240 29 1302.2 0 29 241 54 1302.2 0 54 242 35 1302.2 0 35 243 51 1302.2 0 51 244 79 1302.2 0 79 245 96 1302.2 0 96 246 62 1302.2 0 62 247 37 1302.2 0 37 248 39 1302.2 0 39 249 32 1302.2 0 32 250 57 1302.2 0 57 251 123 1302.2 0 123 252 141 1302.2 0 141 253 188 1302.2 0 188 254 137 1302.2 0 137 255 190 1302.2 0 190 256 106 1302.2 0 106 257 55 1302.2 0 55 258 47 1302.2 0 47 259 27 1302.2 0 27 260 45 1302.2 0 45 261 38 1302.2 0 38 262 90 1302.2 0 90 263 114 1302.2 0 114 264 207 1302.2 0 207 265 131 1302.2 0 131 266 123 1302.2 0 123 267 43 1302.2 0 43 268 28 1302.2 0 28 269 49 1302.2 0 49 270 21 1302.2 0 21 271 65 1302.2 0 65 272 68 1302.2 0 68 273 176 1302.2 0 176 274 113 1302.2 0 113 275 101 1302.2 0 101 276 104 1302.2 0 104 277 77 1302.2 0 77 278 102 1302.2 0 102 279 139 1302.2 0 139 280 165 1302.2 0 165 281 93 1302.2 0 93 282 97 1302.2 0 97 283 217 1302.2 0 217 284 116 1302.2 0 116 285 69 1302.2 0 69 286 57 1302.2 0 57 287 46 1302.2 0 46 288 36 1302.2 0 36 289 48 1302.2 0 48 290 45 1302.2 0 45 291 105 1302.2 0 105 292 272 1302.2 0 272 293 182 1302.2 0 182 294 97 1302.2 0 97 295 28 1302.2 0 28 296 18 1302.2 0 18 297 15 1302.2 0 15 298 50 1302.2 0 50 299 79 1302.2 0 79 300 108 1302.2 0 108 301 99 1302.2 0 99 Finished in 5.47 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 119,604 Reads with adapters: 199 (0.2%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 177 (0.1%) Total basepairs processed: 26,264,401 bp Total written (filtered): 45,048 bp (0.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 199 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 1 0 1 17 132 0.0 3 131 1 18 10 0.0 3 0 0 10 19 8 0.0 3 1 0 4 3 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 53 14 0.0 3 0 0 0 14 56 2 0.0 3 0 0 0 2 80 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 145 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 165 6 0.0 3 0 0 0 6 168 2 0.0 3 0 0 0 2 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 2 0.0 3 0 0 0 2 239 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (56 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 177 Reads with adapters: 5 (2.8%) Reads written (passing filters): 177 (100.0%) Total basepairs processed: 45,048 bp Total written (filtered): 45,033 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 60.0% G: 20.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 2.8 0 5