This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 170.03 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 124,213 (2.3%) Reads that were too short: 17,895 (0.3%) Reads written (passing filters): 106,318 (2.0%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 28,184,256 bp (1.8%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 124213 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1590 1302.2 0 1590 7 69822 1302.2 0 69822 8 332 1302.2 0 332 9 7038 1302.2 0 7038 10 159 1302.2 0 159 11 10 1302.2 0 10 12 18 1302.2 0 18 13 10 1302.2 0 10 14 12 1302.2 0 12 15 8 1302.2 0 8 16 47 1302.2 0 47 17 1877 1302.2 0 1877 18 705 1302.2 0 705 19 19 1302.2 0 19 20 10 1302.2 0 10 21 39 1302.2 0 39 22 618 1302.2 0 618 23 347 1302.2 0 347 24 3 1302.2 0 3 25 8 1302.2 0 8 26 30 1302.2 0 30 27 25 1302.2 0 25 28 103 1302.2 0 103 29 53 1302.2 0 53 30 497 1302.2 0 497 31 264 1302.2 0 264 32 11 1302.2 0 11 33 30 1302.2 0 30 34 12 1302.2 0 12 35 93 1302.2 0 93 36 53 1302.2 0 53 37 21 1302.2 0 21 38 8 1302.2 0 8 39 13 1302.2 0 13 40 13 1302.2 0 13 41 10 1302.2 0 10 42 35 1302.2 0 35 43 6 1302.2 0 6 44 13 1302.2 0 13 45 71 1302.2 0 71 46 6 1302.2 0 6 47 5 1302.2 0 5 48 5 1302.2 0 5 49 18 1302.2 0 18 50 11 1302.2 0 11 51 29 1302.2 0 29 52 8 1302.2 0 8 53 24 1302.2 0 24 54 19 1302.2 0 19 55 16 1302.2 0 16 56 20 1302.2 0 20 57 16 1302.2 0 16 58 15 1302.2 0 15 59 12 1302.2 0 12 60 24 1302.2 0 24 61 20 1302.2 0 20 62 12 1302.2 0 12 63 4 1302.2 0 4 64 5 1302.2 0 5 65 10 1302.2 0 10 66 26 1302.2 0 26 67 32 1302.2 0 32 68 48 1302.2 0 48 69 138 1302.2 0 138 70 9 1302.2 0 9 71 40 1302.2 0 40 72 3 1302.2 0 3 73 18 1302.2 0 18 74 9 1302.2 0 9 75 41 1302.2 0 41 76 42 1302.2 0 42 77 97 1302.2 0 97 78 182 1302.2 0 182 79 216 1302.2 0 216 80 33 1302.2 0 33 81 194 1302.2 0 194 82 380 1302.2 0 380 83 97 1302.2 0 97 84 228 1302.2 0 228 85 259 1302.2 0 259 86 54 1302.2 0 54 87 53 1302.2 0 53 88 304 1302.2 0 304 89 34 1302.2 0 34 90 23 1302.2 0 23 91 252 1302.2 0 252 92 122 1302.2 0 122 93 83 1302.2 0 83 94 177 1302.2 0 177 95 50 1302.2 0 50 96 57 1302.2 0 57 97 118 1302.2 0 118 98 225 1302.2 0 225 99 255 1302.2 0 255 100 29 1302.2 0 29 101 24 1302.2 0 24 102 11 1302.2 0 11 103 14 1302.2 0 14 104 16 1302.2 0 16 105 18 1302.2 0 18 106 9 1302.2 0 9 107 11 1302.2 0 11 108 22 1302.2 0 22 109 17 1302.2 0 17 110 8 1302.2 0 8 111 32 1302.2 0 32 112 43 1302.2 0 43 113 38 1302.2 0 38 114 227 1302.2 0 227 115 216 1302.2 0 216 116 309 1302.2 0 309 117 64 1302.2 0 64 118 48 1302.2 0 48 119 35 1302.2 0 35 120 53 1302.2 0 53 121 57 1302.2 0 57 122 70 1302.2 0 70 123 56 1302.2 0 56 124 56 1302.2 0 56 125 69 1302.2 0 69 126 50 1302.2 0 50 127 50 1302.2 0 50 128 58 1302.2 0 58 129 130 1302.2 0 130 130 154 1302.2 0 154 131 89 1302.2 0 89 132 119 1302.2 0 119 133 148 1302.2 0 148 134 157 1302.2 0 157 135 173 1302.2 0 173 136 148 1302.2 0 148 137 149 1302.2 0 149 138 202 1302.2 0 202 139 133 1302.2 0 133 140 125 1302.2 0 125 141 162 1302.2 0 162 142 156 1302.2 0 156 143 803 1302.2 0 803 144 1044 1302.2 0 1044 145 3144 1302.2 0 3144 146 90 1302.2 0 90 147 118 1302.2 0 118 148 144 1302.2 0 144 149 800 1302.2 0 800 150 880 1302.2 0 880 151 292 1302.2 0 292 152 135 1302.2 0 135 153 70 1302.2 0 70 154 138 1302.2 0 138 155 126 1302.2 0 126 156 150 1302.2 0 150 157 148 1302.2 0 148 158 110 1302.2 0 110 159 121 1302.2 0 121 160 182 1302.2 0 182 161 201 1302.2 0 201 162 164 1302.2 0 164 163 160 1302.2 0 160 164 181 1302.2 0 181 165 149 1302.2 0 149 166 183 1302.2 0 183 167 155 1302.2 0 155 168 208 1302.2 0 208 169 110 1302.2 0 110 170 80 1302.2 0 80 171 96 1302.2 0 96 172 201 1302.2 0 201 173 80 1302.2 0 80 174 61 1302.2 0 61 175 53 1302.2 0 53 176 76 1302.2 0 76 177 87 1302.2 0 87 178 117 1302.2 0 117 179 103 1302.2 0 103 180 115 1302.2 0 115 181 159 1302.2 0 159 182 197 1302.2 0 197 183 276 1302.2 0 276 184 266 1302.2 0 266 185 168 1302.2 0 168 186 131 1302.2 0 131 187 57 1302.2 0 57 188 88 1302.2 0 88 189 186 1302.2 0 186 190 168 1302.2 0 168 191 325 1302.2 0 325 192 177 1302.2 0 177 193 121 1302.2 0 121 194 94 1302.2 0 94 195 67 1302.2 0 67 196 265 1302.2 0 265 197 172 1302.2 0 172 198 50 1302.2 0 50 199 29 1302.2 0 29 200 83 1302.2 0 83 201 198 1302.2 0 198 202 139 1302.2 0 139 203 105 1302.2 0 105 204 129 1302.2 0 129 205 167 1302.2 0 167 206 134 1302.2 0 134 207 103 1302.2 0 103 208 163 1302.2 0 163 209 213 1302.2 0 213 210 195 1302.2 0 195 211 314 1302.2 0 314 212 197 1302.2 0 197 213 250 1302.2 0 250 214 257 1302.2 0 257 215 305 1302.2 0 305 216 290 1302.2 0 290 217 251 1302.2 0 251 218 165 1302.2 0 165 219 106 1302.2 0 106 220 114 1302.2 0 114 221 249 1302.2 0 249 222 286 1302.2 0 286 223 151 1302.2 0 151 224 154 1302.2 0 154 225 260 1302.2 0 260 226 214 1302.2 0 214 227 192 1302.2 0 192 228 176 1302.2 0 176 229 144 1302.2 0 144 230 115 1302.2 0 115 231 81 1302.2 0 81 232 166 1302.2 0 166 233 295 1302.2 0 295 234 210 1302.2 0 210 235 121 1302.2 0 121 236 173 1302.2 0 173 237 179 1302.2 0 179 238 100 1302.2 0 100 239 87 1302.2 0 87 240 140 1302.2 0 140 241 160 1302.2 0 160 242 169 1302.2 0 169 243 159 1302.2 0 159 244 167 1302.2 0 167 245 208 1302.2 0 208 246 205 1302.2 0 205 247 114 1302.2 0 114 248 103 1302.2 0 103 249 345 1302.2 0 345 250 322 1302.2 0 322 251 265 1302.2 0 265 252 110 1302.2 0 110 253 192 1302.2 0 192 254 304 1302.2 0 304 255 418 1302.2 0 418 256 113 1302.2 0 113 257 58 1302.2 0 58 258 66 1302.2 0 66 259 87 1302.2 0 87 260 72 1302.2 0 72 261 85 1302.2 0 85 262 94 1302.2 0 94 263 92 1302.2 0 92 264 112 1302.2 0 112 265 178 1302.2 0 178 266 133 1302.2 0 133 267 102 1302.2 0 102 268 153 1302.2 0 153 269 749 1302.2 0 749 270 984 1302.2 0 984 271 224 1302.2 0 224 272 132 1302.2 0 132 273 145 1302.2 0 145 274 169 1302.2 0 169 275 439 1302.2 0 439 276 163 1302.2 0 163 277 145 1302.2 0 145 278 122 1302.2 0 122 279 104 1302.2 0 104 280 109 1302.2 0 109 281 138 1302.2 0 138 282 122 1302.2 0 122 283 122 1302.2 0 122 284 99 1302.2 0 99 285 95 1302.2 0 95 286 91 1302.2 0 91 287 79 1302.2 0 79 288 130 1302.2 0 130 289 172 1302.2 0 172 290 185 1302.2 0 185 291 183 1302.2 0 183 292 167 1302.2 0 167 293 103 1302.2 0 103 294 110 1302.2 0 110 295 131 1302.2 0 131 296 108 1302.2 0 108 297 53 1302.2 0 53 298 265 1302.2 0 265 299 207 1302.2 0 207 300 100 1302.2 0 100 301 74 1302.2 0 74 Finished in 5.50 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 106,318 Reads with adapters: 71,414 (67.2%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 71,395 (67.2%) Total basepairs processed: 28,184,256 bp Total written (filtered): 19,557,737 bp (69.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 71414 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 18 26 0.0 3 0 0 23 3 19 923 0.0 3 0 875 33 12 3 20 70168 0.0 4 68281 1652 174 54 7 21 151 0.0 4 37 110 1 1 2 22 24 0.0 4 10 3 7 3 1 23 43 0.0 4 3 0 0 35 5 24 1 0.0 4 0 0 0 1 25 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 82 2 0.0 4 0 0 0 0 2 83 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 117 2 0.0 4 0 0 0 1 1 121 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 44 0.0 4 0 0 0 0 44 131 3 0.0 4 0 0 0 0 3 145 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.01 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 71,395 Reads with adapters: 1,335 (1.9%) Reads written (passing filters): 71,395 (100.0%) Total basepairs processed: 19,557,737 bp Total written (filtered): 19,540,669 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1335 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 3.6% G: 89.8% T: 2.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 22 1115.5 0 22 4 6 278.9 0 6 5 1 69.7 0 1 9 3 0.3 0 0 3 10 6 0.1 1 0 6 11 1152 0.0 1 5 1147 12 124 0.0 1 2 122 15 7 0.0 1 0 7 16 1 0.0 1 0 1 127 1 0.0 1 1 140 1 0.0 1 1 181 1 0.0 1 1 183 4 0.0 1 4 225 4 0.0 1 4 258 1 0.0 1 1 267 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.