This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 168.17 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 239,245 (4.5%) Reads that were too short: 50,496 (0.9%) Reads written (passing filters): 188,749 (3.5%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 45,941,707 bp (2.9%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 239245 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 722 1302.2 0 722 7 530 1302.2 0 530 8 1245 1302.2 0 1245 9 71325 1302.2 0 71325 10 139 1302.2 0 139 11 22 1302.2 0 22 12 18 1302.2 0 18 13 18 1302.2 0 18 14 21 1302.2 0 21 15 33 1302.2 0 33 16 26 1302.2 0 26 17 33 1302.2 0 33 18 37 1302.2 0 37 19 26 1302.2 0 26 20 34 1302.2 0 34 21 26 1302.2 0 26 22 29 1302.2 0 29 23 26 1302.2 0 26 24 39 1302.2 0 39 25 27 1302.2 0 27 26 49 1302.2 0 49 27 41 1302.2 0 41 28 63 1302.2 0 63 29 104 1302.2 0 104 30 93 1302.2 0 93 31 1143 1302.2 0 1143 32 99 1302.2 0 99 33 158 1302.2 0 158 34 48 1302.2 0 48 35 64 1302.2 0 64 36 67 1302.2 0 67 37 67 1302.2 0 67 38 141 1302.2 0 141 39 93 1302.2 0 93 40 94 1302.2 0 94 41 125 1302.2 0 125 42 2156 1302.2 0 2156 43 3013 1302.2 0 3013 44 3475 1302.2 0 3475 45 60 1302.2 0 60 46 35 1302.2 0 35 47 68 1302.2 0 68 48 183 1302.2 0 183 49 59 1302.2 0 59 50 98 1302.2 0 98 51 54 1302.2 0 54 52 40 1302.2 0 40 53 79 1302.2 0 79 54 68 1302.2 0 68 55 63 1302.2 0 63 56 43 1302.2 0 43 57 62 1302.2 0 62 58 124 1302.2 0 124 59 163 1302.2 0 163 60 236 1302.2 0 236 61 447 1302.2 0 447 62 463 1302.2 0 463 63 979 1302.2 0 979 64 2334 1302.2 0 2334 65 32599 1302.2 0 32599 66 9480 1302.2 0 9480 67 19129 1302.2 0 19129 68 113 1302.2 0 113 69 40 1302.2 0 40 70 20 1302.2 0 20 71 28 1302.2 0 28 72 31 1302.2 0 31 73 22 1302.2 0 22 74 25 1302.2 0 25 75 29 1302.2 0 29 76 24 1302.2 0 24 77 20 1302.2 0 20 78 25 1302.2 0 25 79 27 1302.2 0 27 80 74 1302.2 0 74 81 116 1302.2 0 116 82 208 1302.2 0 208 83 77 1302.2 0 77 84 71 1302.2 0 71 85 59 1302.2 0 59 86 97 1302.2 0 97 87 421 1302.2 0 421 88 76 1302.2 0 76 89 42 1302.2 0 42 90 117 1302.2 0 117 91 110 1302.2 0 110 92 98 1302.2 0 98 93 49 1302.2 0 49 94 50 1302.2 0 50 95 72 1302.2 0 72 96 214 1302.2 0 214 97 764 1302.2 0 764 98 198 1302.2 0 198 99 69 1302.2 0 69 100 75 1302.2 0 75 101 206 1302.2 0 206 102 780 1302.2 0 780 103 744 1302.2 0 744 104 382 1302.2 0 382 105 53 1302.2 0 53 106 101 1302.2 0 101 107 146 1302.2 0 146 108 278 1302.2 0 278 109 42 1302.2 0 42 110 55 1302.2 0 55 111 119 1302.2 0 119 112 322 1302.2 0 322 113 178 1302.2 0 178 114 127 1302.2 0 127 115 174 1302.2 0 174 116 92 1302.2 0 92 117 138 1302.2 0 138 118 132 1302.2 0 132 119 322 1302.2 0 322 120 299 1302.2 0 299 121 330 1302.2 0 330 122 294 1302.2 0 294 123 192 1302.2 0 192 124 207 1302.2 0 207 125 183 1302.2 0 183 126 192 1302.2 0 192 127 285 1302.2 0 285 128 414 1302.2 0 414 129 188 1302.2 0 188 130 98 1302.2 0 98 131 112 1302.2 0 112 132 262 1302.2 0 262 133 772 1302.2 0 772 134 777 1302.2 0 777 135 365 1302.2 0 365 136 414 1302.2 0 414 137 545 1302.2 0 545 138 1106 1302.2 0 1106 139 794 1302.2 0 794 140 584 1302.2 0 584 141 688 1302.2 0 688 142 384 1302.2 0 384 143 261 1302.2 0 261 144 181 1302.2 0 181 145 121 1302.2 0 121 146 226 1302.2 0 226 147 287 1302.2 0 287 148 258 1302.2 0 258 149 359 1302.2 0 359 150 353 1302.2 0 353 151 180 1302.2 0 180 152 80 1302.2 0 80 153 68 1302.2 0 68 154 144 1302.2 0 144 155 238 1302.2 0 238 156 206 1302.2 0 206 157 174 1302.2 0 174 158 295 1302.2 0 295 159 737 1302.2 0 737 160 447 1302.2 0 447 161 386 1302.2 0 386 162 310 1302.2 0 310 163 345 1302.2 0 345 164 368 1302.2 0 368 165 316 1302.2 0 316 166 325 1302.2 0 325 167 293 1302.2 0 293 168 405 1302.2 0 405 169 198 1302.2 0 198 170 427 1302.2 0 427 171 529 1302.2 0 529 172 507 1302.2 0 507 173 240 1302.2 0 240 174 303 1302.2 0 303 175 348 1302.2 0 348 176 303 1302.2 0 303 177 241 1302.2 0 241 178 150 1302.2 0 150 179 153 1302.2 0 153 180 225 1302.2 0 225 181 350 1302.2 0 350 182 471 1302.2 0 471 183 413 1302.2 0 413 184 501 1302.2 0 501 185 521 1302.2 0 521 186 638 1302.2 0 638 187 465 1302.2 0 465 188 436 1302.2 0 436 189 282 1302.2 0 282 190 157 1302.2 0 157 191 162 1302.2 0 162 192 239 1302.2 0 239 193 208 1302.2 0 208 194 431 1302.2 0 431 195 481 1302.2 0 481 196 237 1302.2 0 237 197 108 1302.2 0 108 198 120 1302.2 0 120 199 288 1302.2 0 288 200 945 1302.2 0 945 201 637 1302.2 0 637 202 157 1302.2 0 157 203 124 1302.2 0 124 204 201 1302.2 0 201 205 269 1302.2 0 269 206 728 1302.2 0 728 207 1264 1302.2 0 1264 208 719 1302.2 0 719 209 391 1302.2 0 391 210 284 1302.2 0 284 211 427 1302.2 0 427 212 362 1302.2 0 362 213 302 1302.2 0 302 214 456 1302.2 0 456 215 670 1302.2 0 670 216 774 1302.2 0 774 217 924 1302.2 0 924 218 506 1302.2 0 506 219 734 1302.2 0 734 220 1006 1302.2 0 1006 221 580 1302.2 0 580 222 241 1302.2 0 241 223 187 1302.2 0 187 224 588 1302.2 0 588 225 806 1302.2 0 806 226 548 1302.2 0 548 227 459 1302.2 0 459 228 549 1302.2 0 549 229 522 1302.2 0 522 230 655 1302.2 0 655 231 860 1302.2 0 860 232 472 1302.2 0 472 233 225 1302.2 0 225 234 128 1302.2 0 128 235 229 1302.2 0 229 236 501 1302.2 0 501 237 512 1302.2 0 512 238 468 1302.2 0 468 239 431 1302.2 0 431 240 585 1302.2 0 585 241 383 1302.2 0 383 242 255 1302.2 0 255 243 299 1302.2 0 299 244 588 1302.2 0 588 245 527 1302.2 0 527 246 564 1302.2 0 564 247 504 1302.2 0 504 248 551 1302.2 0 551 249 821 1302.2 0 821 250 280 1302.2 0 280 251 92 1302.2 0 92 252 106 1302.2 0 106 253 242 1302.2 0 242 254 674 1302.2 0 674 255 1348 1302.2 0 1348 256 1617 1302.2 0 1617 257 1713 1302.2 0 1713 258 1431 1302.2 0 1431 259 939 1302.2 0 939 260 481 1302.2 0 481 261 293 1302.2 0 293 262 254 1302.2 0 254 263 176 1302.2 0 176 264 361 1302.2 0 361 265 580 1302.2 0 580 266 684 1302.2 0 684 267 895 1302.2 0 895 268 938 1302.2 0 938 269 850 1302.2 0 850 270 351 1302.2 0 351 271 182 1302.2 0 182 272 106 1302.2 0 106 273 125 1302.2 0 125 274 395 1302.2 0 395 275 594 1302.2 0 594 276 657 1302.2 0 657 277 310 1302.2 0 310 278 259 1302.2 0 259 279 210 1302.2 0 210 280 608 1302.2 0 608 281 762 1302.2 0 762 282 492 1302.2 0 492 283 297 1302.2 0 297 284 219 1302.2 0 219 285 322 1302.2 0 322 286 384 1302.2 0 384 287 514 1302.2 0 514 288 773 1302.2 0 773 289 615 1302.2 0 615 290 348 1302.2 0 348 291 134 1302.2 0 134 292 105 1302.2 0 105 293 350 1302.2 0 350 294 848 1302.2 0 848 295 724 1302.2 0 724 296 291 1302.2 0 291 297 199 1302.2 0 199 298 138 1302.2 0 138 299 150 1302.2 0 150 300 237 1302.2 0 237 301 507 1302.2 0 507 Finished in 8.87 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 188,749 Reads with adapters: 73,202 (38.8%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 73,195 (38.8%) Total basepairs processed: 45,941,707 bp Total written (filtered): 20,129,732 bp (43.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 73202 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 24 0.0 3 0 0 21 3 16 547 0.0 3 0 528 17 2 17 72234 0.0 3 70651 1549 30 4 18 178 0.0 3 6 133 36 3 19 66 0.0 3 0 0 32 34 20 127 0.0 3 0 0 0 127 36 1 0.0 3 1 42 1 0.0 3 0 0 1 48 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 116 1 0.0 3 0 1 125 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.35 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 73,195 Reads with adapters: 2,574 (3.5%) Reads written (passing filters): 73,195 (100.0%) Total basepairs processed: 20,129,732 bp Total written (filtered): 20,122,004 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2574 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.5% C: 7.0% G: 32.9% T: 0.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2568 1143.7 0 2568 4 6 285.9 0 6