This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 172.11 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 197,533 (3.7%) Reads that were too short: 61,830 (1.2%) Reads written (passing filters): 135,703 (2.5%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 32,648,037 bp (2.0%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 197533 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 852 1302.2 0 852 7 1123 1302.2 0 1123 8 29110 1302.2 0 29110 9 51413 1302.2 0 51413 10 242 1302.2 0 242 11 16 1302.2 0 16 12 11 1302.2 0 11 13 7 1302.2 0 7 14 7 1302.2 0 7 15 2 1302.2 0 2 16 8 1302.2 0 8 17 4 1302.2 0 4 18 11 1302.2 0 11 19 3 1302.2 0 3 20 4 1302.2 0 4 21 4 1302.2 0 4 22 4 1302.2 0 4 23 19 1302.2 0 19 24 7 1302.2 0 7 25 4 1302.2 0 4 26 10 1302.2 0 10 27 5 1302.2 0 5 28 126 1302.2 0 126 29 21 1302.2 0 21 30 22 1302.2 0 22 31 17 1302.2 0 17 32 33 1302.2 0 33 33 39 1302.2 0 39 34 22 1302.2 0 22 35 4 1302.2 0 4 36 9 1302.2 0 9 37 23 1302.2 0 23 38 36 1302.2 0 36 39 1 1302.2 0 1 40 10 1302.2 0 10 41 7 1302.2 0 7 42 47 1302.2 0 47 43 18 1302.2 0 18 44 12 1302.2 0 12 45 4 1302.2 0 4 46 8 1302.2 0 8 47 8 1302.2 0 8 48 5 1302.2 0 5 49 11 1302.2 0 11 50 18 1302.2 0 18 51 9 1302.2 0 9 52 22 1302.2 0 22 53 4 1302.2 0 4 54 10 1302.2 0 10 55 26 1302.2 0 26 56 24 1302.2 0 24 57 10 1302.2 0 10 58 21 1302.2 0 21 59 55 1302.2 0 55 60 455 1302.2 0 455 61 126 1302.2 0 126 62 217 1302.2 0 217 63 9 1302.2 0 9 64 8 1302.2 0 8 65 8 1302.2 0 8 66 37 1302.2 0 37 67 29 1302.2 0 29 68 55 1302.2 0 55 69 6 1302.2 0 6 70 19 1302.2 0 19 71 54 1302.2 0 54 72 15 1302.2 0 15 73 43 1302.2 0 43 74 19 1302.2 0 19 75 31 1302.2 0 31 76 40 1302.2 0 40 77 88 1302.2 0 88 78 49 1302.2 0 49 79 333 1302.2 0 333 80 258 1302.2 0 258 81 247 1302.2 0 247 82 1573 1302.2 0 1573 83 491 1302.2 0 491 84 435 1302.2 0 435 85 229 1302.2 0 229 86 377 1302.2 0 377 87 142 1302.2 0 142 88 51 1302.2 0 51 89 458 1302.2 0 458 90 48 1302.2 0 48 91 441 1302.2 0 441 92 794 1302.2 0 794 93 215 1302.2 0 215 94 351 1302.2 0 351 95 383 1302.2 0 383 96 108 1302.2 0 108 97 1024 1302.2 0 1024 98 153 1302.2 0 153 99 186 1302.2 0 186 100 32 1302.2 0 32 101 67 1302.2 0 67 102 50 1302.2 0 50 103 122 1302.2 0 122 104 81 1302.2 0 81 105 48 1302.2 0 48 106 76 1302.2 0 76 107 54 1302.2 0 54 108 119 1302.2 0 119 109 150 1302.2 0 150 110 32 1302.2 0 32 111 86 1302.2 0 86 112 298 1302.2 0 298 113 446 1302.2 0 446 114 586 1302.2 0 586 115 3053 1302.2 0 3053 116 1333 1302.2 0 1333 117 742 1302.2 0 742 118 156 1302.2 0 156 119 117 1302.2 0 117 120 230 1302.2 0 230 121 273 1302.2 0 273 122 263 1302.2 0 263 123 436 1302.2 0 436 124 289 1302.2 0 289 125 352 1302.2 0 352 126 250 1302.2 0 250 127 350 1302.2 0 350 128 293 1302.2 0 293 129 271 1302.2 0 271 130 356 1302.2 0 356 131 191 1302.2 0 191 132 237 1302.2 0 237 133 316 1302.2 0 316 134 340 1302.2 0 340 135 343 1302.2 0 343 136 338 1302.2 0 338 137 374 1302.2 0 374 138 388 1302.2 0 388 139 397 1302.2 0 397 140 268 1302.2 0 268 141 279 1302.2 0 279 142 369 1302.2 0 369 143 455 1302.2 0 455 144 386 1302.2 0 386 145 272 1302.2 0 272 146 339 1302.2 0 339 147 235 1302.2 0 235 148 335 1302.2 0 335 149 328 1302.2 0 328 150 333 1302.2 0 333 151 452 1302.2 0 452 152 236 1302.2 0 236 153 234 1302.2 0 234 154 315 1302.2 0 315 155 323 1302.2 0 323 156 483 1302.2 0 483 157 350 1302.2 0 350 158 283 1302.2 0 283 159 442 1302.2 0 442 160 444 1302.2 0 444 161 554 1302.2 0 554 162 467 1302.2 0 467 163 500 1302.2 0 500 164 542 1302.2 0 542 165 599 1302.2 0 599 166 966 1302.2 0 966 167 820 1302.2 0 820 168 548 1302.2 0 548 169 345 1302.2 0 345 170 642 1302.2 0 642 171 395 1302.2 0 395 172 676 1302.2 0 676 173 304 1302.2 0 304 174 245 1302.2 0 245 175 306 1302.2 0 306 176 576 1302.2 0 576 177 469 1302.2 0 469 178 322 1302.2 0 322 179 523 1302.2 0 523 180 277 1302.2 0 277 181 527 1302.2 0 527 182 610 1302.2 0 610 183 545 1302.2 0 545 184 602 1302.2 0 602 185 565 1302.2 0 565 186 1093 1302.2 0 1093 187 550 1302.2 0 550 188 582 1302.2 0 582 189 531 1302.2 0 531 190 605 1302.2 0 605 191 468 1302.2 0 468 192 493 1302.2 0 493 193 469 1302.2 0 469 194 557 1302.2 0 557 195 505 1302.2 0 505 196 551 1302.2 0 551 197 515 1302.2 0 515 198 289 1302.2 0 289 199 297 1302.2 0 297 200 569 1302.2 0 569 201 455 1302.2 0 455 202 436 1302.2 0 436 203 307 1302.2 0 307 204 368 1302.2 0 368 205 431 1302.2 0 431 206 535 1302.2 0 535 207 434 1302.2 0 434 208 596 1302.2 0 596 209 503 1302.2 0 503 210 318 1302.2 0 318 211 754 1302.2 0 754 212 562 1302.2 0 562 213 613 1302.2 0 613 214 530 1302.2 0 530 215 478 1302.2 0 478 216 569 1302.2 0 569 217 706 1302.2 0 706 218 540 1302.2 0 540 219 753 1302.2 0 753 220 911 1302.2 0 911 221 529 1302.2 0 529 222 342 1302.2 0 342 223 237 1302.2 0 237 224 254 1302.2 0 254 225 589 1302.2 0 589 226 590 1302.2 0 590 227 557 1302.2 0 557 228 682 1302.2 0 682 229 537 1302.2 0 537 230 500 1302.2 0 500 231 542 1302.2 0 542 232 600 1302.2 0 600 233 517 1302.2 0 517 234 428 1302.2 0 428 235 349 1302.2 0 349 236 1149 1302.2 0 1149 237 1037 1302.2 0 1037 238 826 1302.2 0 826 239 580 1302.2 0 580 240 425 1302.2 0 425 241 386 1302.2 0 386 242 281 1302.2 0 281 243 292 1302.2 0 292 244 277 1302.2 0 277 245 1228 1302.2 0 1228 246 2638 1302.2 0 2638 247 3314 1302.2 0 3314 248 718 1302.2 0 718 249 667 1302.2 0 667 250 466 1302.2 0 466 251 206 1302.2 0 206 252 175 1302.2 0 175 253 170 1302.2 0 170 254 326 1302.2 0 326 255 750 1302.2 0 750 256 1030 1302.2 0 1030 257 826 1302.2 0 826 258 598 1302.2 0 598 259 578 1302.2 0 578 260 730 1302.2 0 730 261 407 1302.2 0 407 262 556 1302.2 0 556 263 319 1302.2 0 319 264 457 1302.2 0 457 265 347 1302.2 0 347 266 986 1302.2 0 986 267 1619 1302.2 0 1619 268 756 1302.2 0 756 269 1241 1302.2 0 1241 270 1020 1302.2 0 1020 271 636 1302.2 0 636 272 645 1302.2 0 645 273 456 1302.2 0 456 274 458 1302.2 0 458 275 615 1302.2 0 615 276 636 1302.2 0 636 277 409 1302.2 0 409 278 344 1302.2 0 344 279 364 1302.2 0 364 280 609 1302.2 0 609 281 643 1302.2 0 643 282 692 1302.2 0 692 283 532 1302.2 0 532 284 567 1302.2 0 567 285 559 1302.2 0 559 286 519 1302.2 0 519 287 562 1302.2 0 562 288 728 1302.2 0 728 289 468 1302.2 0 468 290 711 1302.2 0 711 291 439 1302.2 0 439 292 502 1302.2 0 502 293 578 1302.2 0 578 294 817 1302.2 0 817 295 1148 1302.2 0 1148 296 848 1302.2 0 848 297 499 1302.2 0 499 298 260 1302.2 0 260 299 259 1302.2 0 259 300 290 1302.2 0 290 301 59 1302.2 0 59 Finished in 7.07 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 135,703 Reads with adapters: 53,674 (39.6%) Reads that were too short: 45 (0.0%) Reads written (passing filters): 53,629 (39.5%) Total basepairs processed: 32,648,037 bp Total written (filtered): 14,583,962 bp (44.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 53674 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 28 0.0 3 0 0 19 6 3 19 746 0.0 3 0 696 23 9 18 20 52456 0.0 4 50607 1513 231 67 38 21 133 0.0 4 27 97 5 3 1 22 154 0.0 4 148 3 2 0 1 23 42 0.0 4 0 2 0 33 7 24 5 0.0 4 1 0 0 0 4 28 1 0.0 4 0 0 0 0 1 30 2 0.0 4 1 0 0 0 1 31 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 1 35 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 2 0.0 4 0 0 0 0 2 52 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 0 0 0 2 70 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 81 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 1 101 2 0.0 4 0 0 0 0 2 107 1 0.0 4 0 0 0 0 1 114 2 0.0 4 0 0 0 0 2 118 2 0.0 4 0 0 0 0 2 123 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 49 0.0 4 0 0 0 0 49 131 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 151 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 289 2 0.0 4 0 0 0 0 2 290 1 0.0 4 0 0 0 0 1 291 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.28 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 53,629 Reads with adapters: 4,752 (8.9%) Reads written (passing filters): 53,629 (100.0%) Total basepairs processed: 14,583,962 bp Total written (filtered): 13,922,525 bp (95.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4752 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 53.7% C: 0.9% G: 44.3% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 838.0 0 1 4 1 209.5 0 1 8 2 0.8 0 2 9 477 0.2 0 24 453 10 1096 0.1 1 44 1052 11 682 0.0 1 3 679 12 2 0.0 1 0 2 13 4 0.0 1 0 4 150 1 0.0 1 1 152 1 0.0 1 1 208 9 0.0 1 7 2 209 2 0.0 1 2 256 8 0.0 1 8 257 2349 0.0 1 2203 146 258 78 0.0 1 71 7 259 17 0.0 1 16 1 260 19 0.0 1 19 261 3 0.0 1 3