This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 179.22 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 5,333,634 Reads with adapters: 1,085,626 (20.4%) Reads that were too short: 517,770 (9.7%) Reads written (passing filters): 567,856 (10.6%) Total basepairs processed: 1,605,423,834 bp Total written (filtered): 92,231,100 bp (5.7%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 1085626 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 139 1302.2 0 139 7 1986 1302.2 0 1986 8 2126 1302.2 0 2126 9 135 1302.2 0 135 10 387 1302.2 0 387 11 14146 1302.2 0 14146 12 65 1302.2 0 65 13 23 1302.2 0 23 14 20 1302.2 0 20 15 21 1302.2 0 21 16 24 1302.2 0 24 17 10 1302.2 0 10 18 15 1302.2 0 15 19 13 1302.2 0 13 20 25 1302.2 0 25 21 11 1302.2 0 11 22 28 1302.2 0 28 23 21 1302.2 0 21 24 28 1302.2 0 28 25 14 1302.2 0 14 26 19 1302.2 0 19 27 16 1302.2 0 16 28 14 1302.2 0 14 29 15 1302.2 0 15 30 18 1302.2 0 18 31 18 1302.2 0 18 32 27 1302.2 0 27 33 18 1302.2 0 18 34 27 1302.2 0 27 35 25 1302.2 0 25 36 20 1302.2 0 20 37 19 1302.2 0 19 38 33 1302.2 0 33 39 34 1302.2 0 34 40 14 1302.2 0 14 41 21 1302.2 0 21 42 23 1302.2 0 23 43 37 1302.2 0 37 44 20 1302.2 0 20 45 18 1302.2 0 18 46 14 1302.2 0 14 47 16 1302.2 0 16 48 21 1302.2 0 21 49 22 1302.2 0 22 50 15 1302.2 0 15 51 15 1302.2 0 15 52 20 1302.2 0 20 53 40 1302.2 0 40 54 63 1302.2 0 63 55 26 1302.2 0 26 56 23 1302.2 0 23 57 24 1302.2 0 24 58 21 1302.2 0 21 59 21 1302.2 0 21 60 26 1302.2 0 26 61 20 1302.2 0 20 62 34 1302.2 0 34 63 37 1302.2 0 37 64 140 1302.2 0 140 65 67 1302.2 0 67 66 38 1302.2 0 38 67 47 1302.2 0 47 68 346 1302.2 0 346 69 200 1302.2 0 200 70 340 1302.2 0 340 71 408 1302.2 0 408 72 2743 1302.2 0 2743 73 2236 1302.2 0 2236 74 3534 1302.2 0 3534 75 3900 1302.2 0 3900 76 1551 1302.2 0 1551 77 3117 1302.2 0 3117 78 4972 1302.2 0 4972 79 2815 1302.2 0 2815 80 3418 1302.2 0 3418 81 2337 1302.2 0 2337 82 1969 1302.2 0 1969 83 1348 1302.2 0 1348 84 2663 1302.2 0 2663 85 1100 1302.2 0 1100 86 1841 1302.2 0 1841 87 3505 1302.2 0 3505 88 12096 1302.2 0 12096 89 802 1302.2 0 802 90 1447 1302.2 0 1447 91 9832 1302.2 0 9832 92 2629 1302.2 0 2629 93 5473 1302.2 0 5473 94 2269 1302.2 0 2269 95 1183 1302.2 0 1183 96 579 1302.2 0 579 97 1696 1302.2 0 1696 98 2250 1302.2 0 2250 99 1713 1302.2 0 1713 100 1314 1302.2 0 1314 101 3907 1302.2 0 3907 102 1803 1302.2 0 1803 103 3759 1302.2 0 3759 104 9740 1302.2 0 9740 105 3335 1302.2 0 3335 106 1169 1302.2 0 1169 107 3007 1302.2 0 3007 108 3876 1302.2 0 3876 109 2029 1302.2 0 2029 110 2027 1302.2 0 2027 111 2528 1302.2 0 2528 112 1551 1302.2 0 1551 113 2740 1302.2 0 2740 114 1620 1302.2 0 1620 115 1932 1302.2 0 1932 116 3255 1302.2 0 3255 117 7746 1302.2 0 7746 118 2507 1302.2 0 2507 119 5010 1302.2 0 5010 120 5797 1302.2 0 5797 121 4609 1302.2 0 4609 122 4588 1302.2 0 4588 123 7827 1302.2 0 7827 124 6390 1302.2 0 6390 125 5788 1302.2 0 5788 126 5623 1302.2 0 5623 127 2095 1302.2 0 2095 128 2325 1302.2 0 2325 129 3321 1302.2 0 3321 130 5302 1302.2 0 5302 131 3766 1302.2 0 3766 132 6716 1302.2 0 6716 133 4009 1302.2 0 4009 134 4452 1302.2 0 4452 135 4182 1302.2 0 4182 136 4226 1302.2 0 4226 137 4013 1302.2 0 4013 138 3303 1302.2 0 3303 139 3773 1302.2 0 3773 140 1845 1302.2 0 1845 141 2896 1302.2 0 2896 142 4565 1302.2 0 4565 143 7365 1302.2 0 7365 144 2994 1302.2 0 2994 145 4204 1302.2 0 4204 146 3705 1302.2 0 3705 147 3595 1302.2 0 3595 148 3892 1302.2 0 3892 149 3439 1302.2 0 3439 150 5589 1302.2 0 5589 151 5186 1302.2 0 5186 152 6005 1302.2 0 6005 153 3605 1302.2 0 3605 154 4093 1302.2 0 4093 155 6295 1302.2 0 6295 156 5038 1302.2 0 5038 157 5233 1302.2 0 5233 158 5949 1302.2 0 5949 159 3971 1302.2 0 3971 160 4554 1302.2 0 4554 161 4013 1302.2 0 4013 162 4753 1302.2 0 4753 163 5579 1302.2 0 5579 164 4719 1302.2 0 4719 165 3845 1302.2 0 3845 166 3904 1302.2 0 3904 167 4655 1302.2 0 4655 168 4574 1302.2 0 4574 169 4327 1302.2 0 4327 170 7692 1302.2 0 7692 171 13093 1302.2 0 13093 172 7932 1302.2 0 7932 173 3355 1302.2 0 3355 174 3630 1302.2 0 3630 175 3348 1302.2 0 3348 176 4859 1302.2 0 4859 177 4802 1302.2 0 4802 178 8185 1302.2 0 8185 179 6487 1302.2 0 6487 180 5894 1302.2 0 5894 181 3842 1302.2 0 3842 182 4340 1302.2 0 4340 183 5066 1302.2 0 5066 184 3744 1302.2 0 3744 185 3016 1302.2 0 3016 186 2409 1302.2 0 2409 187 4670 1302.2 0 4670 188 8547 1302.2 0 8547 189 8020 1302.2 0 8020 190 6245 1302.2 0 6245 191 4816 1302.2 0 4816 192 4369 1302.2 0 4369 193 3122 1302.2 0 3122 194 3464 1302.2 0 3464 195 9648 1302.2 0 9648 196 6240 1302.2 0 6240 197 2573 1302.2 0 2573 198 2844 1302.2 0 2844 199 2956 1302.2 0 2956 200 4415 1302.2 0 4415 201 6151 1302.2 0 6151 202 4389 1302.2 0 4389 203 3359 1302.2 0 3359 204 3900 1302.2 0 3900 205 3122 1302.2 0 3122 206 1898 1302.2 0 1898 207 3980 1302.2 0 3980 208 5487 1302.2 0 5487 209 2809 1302.2 0 2809 210 2365 1302.2 0 2365 211 3733 1302.2 0 3733 212 2620 1302.2 0 2620 213 3179 1302.2 0 3179 214 4771 1302.2 0 4771 215 6426 1302.2 0 6426 216 6909 1302.2 0 6909 217 5329 1302.2 0 5329 218 4112 1302.2 0 4112 219 2254 1302.2 0 2254 220 2631 1302.2 0 2631 221 8055 1302.2 0 8055 222 7148 1302.2 0 7148 223 4832 1302.2 0 4832 224 5224 1302.2 0 5224 225 6749 1302.2 0 6749 226 8033 1302.2 0 8033 227 10723 1302.2 0 10723 228 7330 1302.2 0 7330 229 8850 1302.2 0 8850 230 5405 1302.2 0 5405 231 5624 1302.2 0 5624 232 8577 1302.2 0 8577 233 7650 1302.2 0 7650 234 3057 1302.2 0 3057 235 2473 1302.2 0 2473 236 3854 1302.2 0 3854 237 3654 1302.2 0 3654 238 3732 1302.2 0 3732 239 4251 1302.2 0 4251 240 4215 1302.2 0 4215 241 5090 1302.2 0 5090 242 7123 1302.2 0 7123 243 4946 1302.2 0 4946 244 4238 1302.2 0 4238 245 3143 1302.2 0 3143 246 2203 1302.2 0 2203 247 2700 1302.2 0 2700 248 5209 1302.2 0 5209 249 8251 1302.2 0 8251 250 10283 1302.2 0 10283 251 9241 1302.2 0 9241 252 9835 1302.2 0 9835 253 10115 1302.2 0 10115 254 5572 1302.2 0 5572 255 4216 1302.2 0 4216 256 2589 1302.2 0 2589 257 3551 1302.2 0 3551 258 3111 1302.2 0 3111 259 3988 1302.2 0 3988 260 3889 1302.2 0 3889 261 5892 1302.2 0 5892 262 6522 1302.2 0 6522 263 9281 1302.2 0 9281 264 10655 1302.2 0 10655 265 8054 1302.2 0 8054 266 4809 1302.2 0 4809 267 2646 1302.2 0 2646 268 3285 1302.2 0 3285 269 3941 1302.2 0 3941 270 3984 1302.2 0 3984 271 5959 1302.2 0 5959 272 5457 1302.2 0 5457 273 5320 1302.2 0 5320 274 6844 1302.2 0 6844 275 7314 1302.2 0 7314 276 8747 1302.2 0 8747 277 7725 1302.2 0 7725 278 5111 1302.2 0 5111 279 3666 1302.2 0 3666 280 5161 1302.2 0 5161 281 7418 1302.2 0 7418 282 7520 1302.2 0 7520 283 5984 1302.2 0 5984 284 7076 1302.2 0 7076 285 3950 1302.2 0 3950 286 3034 1302.2 0 3034 287 3565 1302.2 0 3565 288 2814 1302.2 0 2814 289 3959 1302.2 0 3959 290 4538 1302.2 0 4538 291 6580 1302.2 0 6580 292 9898 1302.2 0 9898 293 10759 1302.2 0 10759 294 2208 1302.2 0 2208 295 2394 1302.2 0 2394 296 3000 1302.2 0 3000 297 2942 1302.2 0 2942 298 1962 1302.2 0 1962 299 2130 1302.2 0 2130 300 2163 1302.2 0 2163 301 1501 1302.2 0 1501 Finished in 24.18 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 567,856 Reads with adapters: 2,347 (0.4%) Reads that were too short: 194 (0.0%) Reads written (passing filters): 2,153 (0.4%) Total basepairs processed: 92,231,100 bp Total written (filtered): 575,875 bp (0.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2347 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 32 0.0 3 0 25 1 3 3 20 2017 0.0 4 1944 63 4 3 3 21 9 0.0 4 2 4 0 2 1 22 11 0.0 4 0 0 0 1 10 23 5 0.0 4 0 0 0 2 3 25 3 0.0 4 0 0 0 0 3 26 1 0.0 4 0 0 0 0 1 27 2 0.0 4 0 0 0 0 2 28 2 0.0 4 0 0 0 0 2 29 3 0.0 4 0 0 0 0 3 30 3 0.0 4 0 0 0 0 3 32 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 35 2 0.0 4 0 0 0 0 2 36 5 0.0 4 0 0 0 0 5 37 3 0.0 4 0 0 0 0 3 38 3 0.0 4 0 0 0 0 3 39 4 0.0 4 0 0 0 0 4 40 2 0.0 4 0 0 0 0 2 41 1 0.0 4 0 0 0 0 1 42 2 0.0 4 0 0 0 0 2 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 2 0.0 4 0 0 0 0 2 46 2 0.0 4 0 0 0 0 2 47 4 0.0 4 0 0 0 0 4 48 1 0.0 4 0 0 0 0 1 49 4 0.0 4 0 0 0 1 3 50 4 0.0 4 0 0 0 0 4 51 2 0.0 4 0 0 0 0 2 52 4 0.0 4 0 0 0 0 4 53 3 0.0 4 0 0 0 0 3 55 2 0.0 4 0 0 0 0 2 56 3 0.0 4 0 0 0 0 3 57 1 0.0 4 0 0 0 0 1 59 2 0.0 4 0 0 0 0 2 60 1 0.0 4 0 0 0 0 1 61 3 0.0 4 0 0 0 0 3 62 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 64 2 0.0 4 0 0 0 0 2 65 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 0 0 2 67 2 0.0 4 0 0 0 1 1 68 2 0.0 4 0 0 0 0 2 70 4 0.0 4 0 0 0 0 4 71 2 0.0 4 0 0 0 0 2 72 1 0.0 4 0 0 0 0 1 73 4 0.0 4 0 0 0 0 4 74 2 0.0 4 0 0 0 0 2 75 2 0.0 4 0 0 0 0 2 76 2 0.0 4 0 0 0 0 2 78 4 0.0 4 0 0 0 0 4 79 2 0.0 4 0 0 0 0 2 80 3 0.0 4 0 0 0 0 3 81 3 0.0 4 0 0 0 0 3 82 3 0.0 4 0 0 0 0 3 83 4 0.0 4 0 0 0 0 4 84 1 0.0 4 0 0 0 0 1 85 2 0.0 4 0 0 0 1 1 86 1 0.0 4 0 0 0 0 1 87 2 0.0 4 0 0 0 0 2 88 2 0.0 4 0 0 0 0 2 89 1 0.0 4 0 0 0 0 1 90 2 0.0 4 0 0 0 0 2 91 2 0.0 4 0 0 0 0 2 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 0 2 95 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 0 0 1 97 3 0.0 4 0 0 0 0 3 98 2 0.0 4 0 0 0 0 2 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 102 2 0.0 4 0 0 0 0 2 103 4 0.0 4 0 0 0 1 3 107 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 0 1 112 4 0.0 4 0 0 0 0 4 113 1 0.0 4 0 0 0 0 1 114 2 0.0 4 0 0 0 0 2 116 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 118 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 123 2 0.0 4 0 0 0 0 2 124 3 0.0 4 0 0 0 0 3 125 7 0.0 4 0 0 0 0 7 127 1 0.0 4 0 0 0 0 1 128 2 0.0 4 0 0 0 0 2 130 3 0.0 4 0 0 0 0 3 131 2 0.0 4 0 0 0 0 2 133 4 0.0 4 0 0 0 0 4 134 1 0.0 4 0 0 0 0 1 137 2 0.0 4 0 0 0 0 2 138 3 0.0 4 0 0 0 0 3 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 1 141 1 0.0 4 0 0 0 0 1 143 3 0.0 4 0 0 0 0 3 144 1 0.0 4 0 0 0 0 1 145 2 0.0 4 0 0 0 0 2 149 3 0.0 4 0 0 0 0 3 150 1 0.0 4 0 0 0 0 1 151 2 0.0 4 0 0 0 0 2 153 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 157 3 0.0 4 0 0 0 0 3 158 1 0.0 4 0 0 0 0 1 159 3 0.0 4 0 0 0 0 3 161 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 2 0.0 4 0 0 0 1 1 168 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 179 2 0.0 4 0 0 0 0 2 180 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 226 3 0.0 4 0 0 0 0 3 228 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 2,153 Reads with adapters: 452 (21.0%) Reads written (passing filters): 2,153 (100.0%) Total basepairs processed: 575,875 bp Total written (filtered): 570,124 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 452 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 1.1% G: 93.6% T: 3.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 1 0.0 0 0 1 10 2 0.0 1 0 2 11 446 0.0 1 3 443 272 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.